2qg9 - Revision history

OCA at 09:16, 21 February 2024

OCA — Wed, 21 Feb 2024 09:16:13 GMT

←Older revision		Revision as of 09:16, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='2qg9' size='340' side='right'caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2qg9' size='340' side='right'caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1nbe\|1nbe]]~~, [[~~1rah~~\|~~1rah]], [[6at1\|6at1]], [[2qgf\|2qgf~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>pyrB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895]), pyrI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [https://pdbe.org/2qg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [https://www.ebi.ac.uk/pdbsum/2qg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qg9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [https://pdbe.org/2qg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [https://www.ebi.ac.uk/pdbsum/2qg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qg9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~PYRI_ECOLI PYRI_ECOLI]] Involved in allosteric regulation of aspartate carbamoyltransferase.[HAMAP-Rule:MF_00002~~]	+	[https://www.uniprot.org/uniprot/PYRB_ECOLI PYRB_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qg9 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qg9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Asp19 and His20 of Escherichia coli aspartate transcarbamoylase (EC 2.1.3.2) function in the binding of the triphosphate and ribose moieties of ATP and CTP and thereby may mediate important heterotropic regulation. The roles of these residues were investigated by individually mutating each of them to alanine and determining both the kinetic parameters and the structures of the mutant enzymes. The structures were determined by X-ray crystallography at 2.15 and 2.75 A resolution for His20Ar and Asp19Ar, respectively. Analysis was carried out on the unliganded T-state form. The structures of the mutants did not show gross structural divergence from the canonical T-state, but showed small and systematic differences that were analyzed by global conformational analysis. Structural analysis and comparison with other regulatory-chain mutants confirmed that the Asp19Ar mutant represents the stabilized T-state, while structural analysis of the His20Ar form indicated that it represents an equilibrium shifted towards the R-state. Global analysis of the Asp19Ar and His20Ar enzymes suggested a possible role as molecular modulators of the heterotropic effects caused by the binding of nucleotides at the regulatory site. These studies highlighted the structural determinants of T- or R-state stabilization. Additionally, application of the ;consensus modeling' methodology combined with high-resolution data allowed the determination of unclear structural features contributing to nucleotide specificity and the role of the N-termini of the regulatory chains.
-
-	Comparison of two T-state structures of regulatory-chain mutants of Escherichia coli aspartate transcarbamoylase suggests that His20 and Asp19 modulate the response to heterotropic effectors.,Stec B, Williams MK, Stieglitz KA, Kantrowitz ER Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1243-53. Epub 2007, Nov 16. PMID:18084072<ref>PMID:18084072</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2qg9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Aspartate carbamoyltransferase 3D structures\|Aspartate carbamoyltransferase 3D structures]]		*[[Aspartate carbamoyltransferase 3D structures\|Aspartate carbamoyltransferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Aspartate carbamoyltransferase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kantrowitz~~, E R~~]]	+	[[Category: Kantrowitz ER]]
-	[[Category: Stec, B]]	+	[[Category: Stec B]]
-	[[Category: Stieglitz~~, K A~~]]	+	[[Category: Stieglitz KA]]
-	[[Category: Williams~~, M K]]~~	+	[[Category: Williams MK]]
-	~~[[Category: Allosteric enzyme]]~~	+
-	~~[[Category: Heterotropic regulation]]~~	+
-	~~[[Category: Regulatory chain mutant]]~~	+
-	~~[[Category: Transferase-transferase regulator complex~~]]	+

OCA at 08:19, 25 June 2021

OCA — Fri, 25 Jun 2021 08:19:07 GMT

←Older revision		Revision as of 08:19, 25 June 2021
Line 1:		Line 1:

	==Structure of a regulatory subunit mutant D19A of ATCase from E. coli==		==Structure of a regulatory subunit mutant D19A of ATCase from E. coli==
-	<StructureSection load='2qg9' size='340' side='right' caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='2qg9' size='340' side='right'caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nbe\|1nbe]], [[1rah\|1rah]], [[6at1\|6at1]], [[2qgf\|2qgf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1nbe\|1nbe]], [[1rah\|1rah]], [[6at1\|6at1]], [[2qgf\|2qgf]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), pyrI ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), pyrI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [~~http~~://pdbe.org/2qg9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2qg9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2qg9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [https://pdbe.org/2qg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [https://www.ebi.ac.uk/pdbsum/2qg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qg9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PYRI_ECOLI PYRI_ECOLI]] Involved in allosteric regulation of aspartate carbamoyltransferase.[HAMAP-Rule:MF_00002]	+	[[https://www.uniprot.org/uniprot/PYRI_ECOLI PYRI_ECOLI]] Involved in allosteric regulation of aspartate carbamoyltransferase.[HAMAP-Rule:MF_00002]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Aspartate carbamoyltransferase\|Aspartate carbamoyltransferase]]	+	*[[Aspartate carbamoyltransferase 3D structures\|Aspartate carbamoyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 40:		Line 40:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Aspartate carbamoyltransferase]]		[[Category: Aspartate carbamoyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Kantrowitz, E R]]		[[Category: Kantrowitz, E R]]
	[[Category: Stec, B]]		[[Category: Stec, B]]

OCA at 11:31, 5 September 2018

OCA — Wed, 05 Sep 2018 11:31:48 GMT

←Older revision		Revision as of 11:31, 5 September 2018
Line 1:		Line 1:
		+
	==Structure of a regulatory subunit mutant D19A of ATCase from E. coli==		==Structure of a regulatory subunit mutant D19A of ATCase from E. coli==
	<StructureSection load='2qg9' size='340' side='right' caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2qg9' size='340' side='right' caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [http://pdbe.org/2qg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [http://www.ebi.ac.uk/pdbsum/2qg9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [http://pdbe.org/2qg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [http://www.ebi.ac.uk/pdbsum/2qg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qg9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qg/2qg9_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qg/2qg9_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:35, 9 February 2016

OCA — Tue, 09 Feb 2016 03:35:57 GMT

←Older revision		Revision as of 03:35, 9 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qg9 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:13, 10 September 2015

OCA — Thu, 10 Sep 2015 18:13:33 GMT

←Older revision		Revision as of 18:13, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2qg9' size='340' side='right' caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2qg9' size='340' side='right' caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nbe\|1nbe]], [[1rah\|1rah]], [[6at1\|6at1]], [[2qgf\|2qgf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nbe\|1nbe]], [[1rah\|1rah]], [[6at1\|6at1]], [[2qgf\|2qgf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [http://www.ebi.ac.uk/pdbsum/2qg9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [http://pdbe.org/2qg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [http://www.ebi.ac.uk/pdbsum/2qg9 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2qg9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Aspartate carbamoyltransferase]]		[[Category: Aspartate carbamoyltransferase]]
-	[[Category: Escherichia coli]]
	[[Category: Kantrowitz, E R]]		[[Category: Kantrowitz, E R]]
	[[Category: Stec, B]]		[[Category: Stec, B]]

OCA at 04:08, 25 December 2014

OCA — Thu, 25 Dec 2014 04:08:02 GMT

←Older revision		Revision as of 04:08, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nbe\|1nbe]], [[1rah\|1rah]], [[6at1\|6at1]], [[2qgf\|2qgf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nbe\|1nbe]], [[1rah\|1rah]], [[6at1\|6at1]], [[2qgf\|2qgf]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [http://www.ebi.ac.uk/pdbsum/2qg9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [http://www.ebi.ac.uk/pdbsum/2qg9 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PYRI_ECOLI PYRI_ECOLI]] Involved in allosteric regulation of aspartate carbamoyltransferase.[HAMAP-Rule:MF_00002]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Aspartate carbamoyltransferase]]		[[Category: Aspartate carbamoyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Kantrowitz, E R.]]	+	[[Category: Kantrowitz, E R]]
-	[[Category: Stec, B.]]	+	[[Category: Stec, B]]
-	[[Category: Stieglitz, K A.]]	+	[[Category: Stieglitz, K A]]
-	[[Category: Williams, M K.]]	+	[[Category: Williams, M K]]
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]
	[[Category: Heterotropic regulation]]		[[Category: Heterotropic regulation]]
	[[Category: Regulatory chain mutant]]		[[Category: Regulatory chain mutant]]
	[[Category: Transferase-transferase regulator complex]]		[[Category: Transferase-transferase regulator complex]]

OCA at 19:43, 30 September 2014

OCA — Tue, 30 Sep 2014 19:43:02 GMT

←Older revision		Revision as of 19:43, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2qg9.~~png~~\|~~left~~\|200px]]	+	==Structure of a regulatory subunit mutant D19A of ATCase from E. coli==
		+	<StructureSection load='2qg9' size='340' side='right' caption='[[2qg9]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2qg9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QG9 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1nbe\|1nbe]], [[1rah\|1rah]], [[6at1\|6at1]], [[2qgf\|2qgf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qg9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qg9 RCSB], [http://www.ebi.ac.uk/pdbsum/2qg9 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qg/2qg9_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Asp19 and His20 of Escherichia coli aspartate transcarbamoylase (EC 2.1.3.2) function in the binding of the triphosphate and ribose moieties of ATP and CTP and thereby may mediate important heterotropic regulation. The roles of these residues were investigated by individually mutating each of them to alanine and determining both the kinetic parameters and the structures of the mutant enzymes. The structures were determined by X-ray crystallography at 2.15 and 2.75 A resolution for His20Ar and Asp19Ar, respectively. Analysis was carried out on the unliganded T-state form. The structures of the mutants did not show gross structural divergence from the canonical T-state, but showed small and systematic differences that were analyzed by global conformational analysis. Structural analysis and comparison with other regulatory-chain mutants confirmed that the Asp19Ar mutant represents the stabilized T-state, while structural analysis of the His20Ar form indicated that it represents an equilibrium shifted towards the R-state. Global analysis of the Asp19Ar and His20Ar enzymes suggested a possible role as molecular modulators of the heterotropic effects caused by the binding of nucleotides at the regulatory site. These studies highlighted the structural determinants of T- or R-state stabilization. Additionally, application of the ;consensus modeling' methodology combined with high-resolution data allowed the determination of unclear structural features contributing to nucleotide specificity and the role of the N-termini of the regulatory chains.

-	~~{{STRUCTURE_2qg9\| PDB=2qg9 \| SCENE= }}~~	+	Comparison of two T-state structures of regulatory-chain mutants of Escherichia coli aspartate transcarbamoylase suggests that His20 and Asp19 modulate the response to heterotropic effectors.,Stec B, Williams MK, Stieglitz KA, Kantrowitz ER Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1243-53. Epub 2007, Nov 16. PMID:18084072<ref>PMID:18084072</ref>

-	~~===Structure of~~ a ~~regulatory subunit mutant D19A~~ of ~~ATCase from E~~. ~~coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_18084072}}~~	+	==See Also==
-		+	*[[Aspartate carbamoyltransferase\|Aspartate carbamoyltransferase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~2qg9~~]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:018084072<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aspartate carbamoyltransferase]]		[[Category: Aspartate carbamoyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]

OCA: Protected "2qg9" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 19:48:07 GMT

Protected "2qg9" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:48, 7 January 2013

OCA at 19:48, 7 January 2013

OCA — Mon, 07 Jan 2013 19:48:04 GMT

←Older revision		Revision as of 19:48, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2qg9.png\|left\|200px]]		[[Image:2qg9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2qg9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2qg9\| PDB=2qg9 \| SCENE= }}		{{STRUCTURE_2qg9\| PDB=2qg9 \| SCENE= }}

	===Structure of a regulatory subunit mutant D19A of ATCase from E. coli===		===Structure of a regulatory subunit mutant D19A of ATCase from E. coli===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18084072}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18084072 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18084072}}		{{ABSTRACT_PUBMED_18084072}}

	==About this Structure==		==About this Structure==
-	~~2QG9~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA].	+	[[2qg9]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18084072~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018084072</ref><references group="xtra"/>
	[[Category: Aspartate carbamoyltransferase]]		[[Category: Aspartate carbamoyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
Line 33:		Line 21:
	[[Category: Heterotropic regulation]]		[[Category: Heterotropic regulation]]
	[[Category: Regulatory chain mutant]]		[[Category: Regulatory chain mutant]]
-	[[Category: Transferase/transferase regulator complex]]	+	[[Category: Transferase-transferase regulator complex]]
-		+
-	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:09:02 2009''~~	+

OCA at 04:09, 17 February 2009

OCA — Tue, 17 Feb 2009 04:09:02 GMT

←Older revision		Revision as of 04:09, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2QG9 is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA].	+	2QG9 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QG9 OCA].

	==Reference==		==Reference==
-	Comparison of two T-state structures of regulatory-chain mutants of Escherichia coli aspartate transcarbamoylase suggests that His20 and Asp19 modulate the response to heterotropic effectors., Stec B, Williams MK, Stieglitz KA, Kantrowitz ER, Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1243-53. Epub 2007, Nov 16. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18084072 18084072]~~	+	<ref group="xtra">PMID:18084072</ref><references group="xtra"/>
	[[Category: Aspartate carbamoyltransferase]]		[[Category: Aspartate carbamoyltransferase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Protein complex]]
	[[Category: Kantrowitz, E R.]]		[[Category: Kantrowitz, E R.]]
	[[Category: Stec, B.]]		[[Category: Stec, B.]]
Line 36:		Line 35:
	[[Category: Transferase/transferase regulator complex]]		[[Category: Transferase/transferase regulator complex]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 09~~:38:~~43 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:09:02 2009''