2qry - Revision history

OCA at 08:31, 25 June 2021

OCA — Fri, 25 Jun 2021 08:31:18 GMT

←Older revision		Revision as of 08:31, 25 June 2021
Line 1:		Line 1:

	==Periplasmic thiamin binding protein==		==Periplasmic thiamin binding protein==
-	<StructureSection load='2qry' size='340' side='right' caption='[[2qry]], [[Resolution\|resolution]] 2.25Å' scene=''>	+	<StructureSection load='2qry' size='340' side='right'caption='[[2qry]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qry]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2QRY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qry]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QRY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPS:THIAMIN+PHOSPHATE'>TPS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TPS:THIAMIN+PHOSPHATE'>TPS</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [~~http~~://pdbe.org/2qry PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2qry PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2qry ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [https://pdbe.org/2qry PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [https://www.ebi.ac.uk/pdbsum/2qry PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qry ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/THIB_ECOLI THIB_ECOLI]] Part of the ABC transporter complex ThiBPQ involved in thiamine import.	+	[[https://www.uniprot.org/uniprot/THIB_ECOLI THIB_ECOLI]] Part of the ABC transporter complex ThiBPQ involved in thiamine import.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]
	[[Category: Soriano, E V]]		[[Category: Soriano, E V]]

OCA at 11:01, 5 September 2018

OCA — Wed, 05 Sep 2018 11:01:38 GMT

←Older revision		Revision as of 11:01, 5 September 2018
Line 1:		Line 1:
		+
	==Periplasmic thiamin binding protein==		==Periplasmic thiamin binding protein==
	<StructureSection load='2qry' size='340' side='right' caption='[[2qry]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2qry' size='340' side='right' caption='[[2qry]], [[Resolution\|resolution]] 2.25Å' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [http://pdbe.org/2qry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [http://www.ebi.ac.uk/pdbsum/2qry PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [http://pdbe.org/2qry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [http://www.ebi.ac.uk/pdbsum/2qry PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qry ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qry_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qry_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:33, 7 February 2016

OCA — Sun, 07 Feb 2016 04:33:30 GMT

←Older revision		Revision as of 04:33, 7 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qry ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:53, 9 September 2015

OCA — Wed, 09 Sep 2015 21:53:11 GMT

←Older revision		Revision as of 21:53, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='2qry' size='340' side='right' caption='[[2qry]], [[Resolution\|resolution]] 2.25Å' scene=''>		<StructureSection load='2qry' size='340' side='right' caption='[[2qry]], [[Resolution\|resolution]] 2.25Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qry]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QRY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qry]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QRY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPS:THIAMIN+PHOSPHATE'>TPS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPS:THIAMIN+PHOSPHATE'>TPS</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [http://www.ebi.ac.uk/pdbsum/2qry PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [http://pdbe.org/2qry PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [http://www.ebi.ac.uk/pdbsum/2qry PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2qry" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]
	[[Category: Soriano, E V]]		[[Category: Soriano, E V]]

OCA at 07:53, 24 December 2014

OCA — Wed, 24 Dec 2014 07:53:20 GMT

←Older revision		Revision as of 07:53, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2qry]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QRY FirstGlance]. <br>		<table><tr><td colspan='2'>[[2qry]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QRY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPS:THIAMIN+PHOSPHATE'>TPS</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPS:THIAMIN+PHOSPHATE'>TPS</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [http://www.ebi.ac.uk/pdbsum/2qry PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [http://www.ebi.ac.uk/pdbsum/2qry PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/THIB_ECOLI THIB_ECOLI]] Part of the ABC transporter complex ThiBPQ involved in thiamine import.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: Soriano, E V.]]	+	[[Category: Soriano, E V]]
	[[Category: Abc transporter]]		[[Category: Abc transporter]]
	[[Category: Periplasmic]]		[[Category: Periplasmic]]
	[[Category: Thiamin binding protein]]		[[Category: Thiamin binding protein]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 18:44, 30 September 2014

OCA — Tue, 30 Sep 2014 18:44:21 GMT

←Older revision		Revision as of 18:44, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2qry.~~png~~\|~~left~~\|200px]]	+	==Periplasmic thiamin binding protein==
		+	<StructureSection load='2qry' size='340' side='right' caption='[[2qry]], [[Resolution\|resolution]] 2.25Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2qry]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QRY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TPS:THIAMIN+PHOSPHATE'>TPS</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">thiB, tbpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qry OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qry RCSB], [http://www.ebi.ac.uk/pdbsum/2qry PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qry_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ATP-binding cassette (ABC) transporters are responsible for the transport of a wide variety of water-soluble molecules and ions into prokaryotic cells. In Gram-negative bacteria, periplasmic-binding proteins deliver ions or molecules such as thiamin to the membrane-bound ABC transporter. The gene for the thiamin-binding protein tbpA has been identified in both Escherichia coli and Salmonella typhimurium. Here we report the crystal structure of TbpA from E. coli with bound thiamin monophosphate. The structure was determined at 2.25 A resolution using single-wavelength anomalous diffraction experiments, despite the presence of nonmerohedral twinning. The crystal structure shows that TbpA belongs to the group II periplasmic-binding protein family. Equilibrium binding measurements showed similar dissociation constants for thiamin, thiamin monophosphate, and thiamin pyrophosphate. Analysis of the binding site by molecular modeling demonstrated how TbpA binds all three forms of thiamin. A comparison of TbpA and thiaminase-I, a thiamin-degrading enzyme, revealed structural similarity between the two proteins, especially in domain 1, suggesting that the two proteins evolved from a common ancestor.

-	~~{{STRUCTURE_2qry\| PDB=2qry \| SCENE= }}~~	+	Structural Similarities between Thiamin-Binding Protein and Thiaminase-I Suggest a Common Ancestor(,).,Soriano EV, Rajashankar KR, Hanes JW, Bale S, Begley TP, Ealick SE Biochemistry. 2008 Feb 5;47(5):1346-57. Epub 2008 Jan 5. PMID:18177053<ref>PMID:18177053</ref>

-	~~===Periplasmic thiamin binding protein===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18177053}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2qry]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018177053<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ealick, S E.]]		[[Category: Ealick, S E.]]

OCA: Protected "2qry" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 18:40:20 GMT

Protected "2qry" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:40, 7 January 2013

OCA at 18:40, 7 January 2013

OCA — Mon, 07 Jan 2013 18:40:19 GMT

←Older revision		Revision as of 18:40, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2qry.png\|left\|200px]]		[[Image:2qry.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2qry", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2qry\| PDB=2qry \| SCENE= }}		{{STRUCTURE_2qry\| PDB=2qry \| SCENE= }}

	===Periplasmic thiamin binding protein===		===Periplasmic thiamin binding protein===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18177053}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18177053 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18177053}}		{{ABSTRACT_PUBMED_18177053}}

	==About this Structure==		==About this Structure==
-	~~2QRY~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA].	+	[[2qry]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18177053~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018177053</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ealick, S E.]]		[[Category: Ealick, S E.]]
Line 31:		Line 19:
	[[Category: Thiamin binding protein]]		[[Category: Thiamin binding protein]]
	[[Category: Transport protein]]		[[Category: Transport protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:21:18 2009''

OCA at 09:21, 16 February 2009

OCA — Mon, 16 Feb 2009 09:21:19 GMT

←Older revision		Revision as of 09:21, 16 February 2009
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	==About this Structure==		==About this Structure==
-	2QRY is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA].	+	2QRY is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRY OCA].

	==Reference==		==Reference==
-	Structural Similarities between Thiamin-Binding Protein and Thiaminase-I Suggest a Common Ancestor(,)., Soriano EV, Rajashankar KR, Hanes JW, Bale S, Begley TP, Ealick SE, Biochemistry. 2008 Feb 5;47(5):1346-57. Epub 2008 Jan 5. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18177053 18177053]~~	+	<ref group="xtra">PMID:18177053</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Ealick, S E.]]		[[Category: Ealick, S E.]]
	[[Category: Soriano, E V.]]		[[Category: Soriano, E V.]]
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	[[Category: Transport protein]]		[[Category: Transport protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 13~~:21:~~24 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:21:18 2009''

OCA at 10:21, 27 July 2008

OCA — Sun, 27 Jul 2008 10:21:28 GMT

←Older revision		Revision as of 10:21, 27 July 2008
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-	[[Image:2qry.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2qry.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2qry\| PDB=2qry \| SCENE= }}		{{STRUCTURE_2qry\| PDB=2qry \| SCENE= }}

-	~~'''~~Periplasmic thiamin binding protein~~'''~~	+	===Periplasmic thiamin binding protein===


-	~~==Overview==~~	+	<!--
-	~~ATP~~-~~binding cassette (ABC) transporters are responsible for the transport of a wide variety of water~~-~~soluble molecules and ions into prokaryotic cells. In Gram-negative bacteria~~, ~~periplasmic-binding proteins deliver ions or molecules such as thiamin~~ to the membrane-bound ABC transporter. The gene for the thiamin-binding protein tbpA has been identified in both Escherichia coli and Salmonella typhimurium. Here we report the crystal structure of TbpA from E. coli with bound thiamin monophosphate. The structure was determined at 2.~~25 A resolution using single-wavelength anomalous diffraction experiments, despite the presence of nonmerohedral twinning~~. ~~The crystal structure shows that TbpA belongs to the group II periplasmic-binding protein family. Equilibrium binding measurements showed similar dissociation constants for thiamin~~, ~~thiamin monophosphate, and thiamin pyrophosphate. Analysis of~~ the ~~binding site by molecular modeling demonstrated how TbpA binds all three forms of thiamin~~. ~~A comparison of TbpA and thiaminase~~-~~I, a thiamin~~-~~degrading enzyme, revealed structural similarity between the two proteins, especially in domain 1, suggesting that the two proteins evolved from a common ancestor.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_18177053}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18177053 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18177053}}

	==About this Structure==		==About this Structure==
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	[[Category: Thiamin binding protein]]		[[Category: Thiamin binding protein]]
	[[Category: Transport protein]]		[[Category: Transport protein]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun ~~May 4 15~~:33:33 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:21:24 2008''