2qyi - Revision history

OCA at 07:08, 10 November 2021

OCA — Wed, 10 Nov 2021 07:08:08 GMT

←Older revision		Revision as of 07:08, 10 November 2021
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	<StructureSection load='2qyi' size='340' side='right'caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2qyi' size='340' side='right'caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin] and [~~http~~://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2QYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin] and [https://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QYI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [~~http~~://pdbe.org/2qyi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2qyi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2qyi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [https://pdbe.org/2qyi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [https://www.ebi.ac.uk/pdbsum/2qyi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qyi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.	+	[[https://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:09, 10 June 2020

OCA — Wed, 10 Jun 2020 07:09:08 GMT

←Older revision		Revision as of 07:09, 10 June 2020
Line 1:		Line 1:

	==Crystal structure of a binary complex between an engineered trypsin inhibitor and Bovine trypsin==		==Crystal structure of a binary complex between an engineered trypsin inhibitor and Bovine trypsin==
-	<StructureSection load='2qyi' size='340' side='right' caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='2qyi' size='340' side='right'caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2QYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2QYI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://pdbe.org/2qyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qyi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://pdbe.org/2qyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qyi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Chymotrypsin ~~Inhibitor~~\|Chymotrypsin ~~Inhibitor~~]]	+	*[[Chymotrypsin inhibitor 3D structures\|Chymotrypsin inhibitor 3D structures]]
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	[[Category: Bovin]]		[[Category: Bovin]]
	[[Category: Dolichos tetragonolobus]]		[[Category: Dolichos tetragonolobus]]
		+	[[Category: Large Structures]]
	[[Category: Dasgupta, J]]		[[Category: Dasgupta, J]]
	[[Category: Dattagupta, J K]]		[[Category: Dattagupta, J K]]

OCA at 10:49, 5 September 2018

OCA — Wed, 05 Sep 2018 10:49:43 GMT

←Older revision		Revision as of 10:49, 5 September 2018
Line 1:		Line 1:
		+
	==Crystal structure of a binary complex between an engineered trypsin inhibitor and Bovine trypsin==		==Crystal structure of a binary complex between an engineered trypsin inhibitor and Bovine trypsin==
	<StructureSection load='2qyi' size='340' side='right' caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2qyi' size='340' side='right' caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://pdbe.org/2qyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://pdbe.org/2qyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qyi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qy/2qyi_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qy/2qyi_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 43:		Line 44:
	[[Category: Sen, U]]		[[Category: Sen, U]]
	[[Category: Bovine trypsin]]		[[Category: Bovine trypsin]]
		+	[[Category: Calcium]]
	[[Category: Complex]]		[[Category: Complex]]
	[[Category: Digestion]]		[[Category: Digestion]]

OCA at 02:02, 8 February 2016

OCA — Mon, 08 Feb 2016 02:02:23 GMT

←Older revision		Revision as of 02:02, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qyi ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:50, 10 September 2015

OCA — Thu, 10 Sep 2015 06:50:10 GMT

←Older revision		Revision as of 06:50, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='2qyi' size='340' side='right' caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2qyi' size='340' side='right' caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~] and [http://en.wikipedia.org/wiki/~~Psophocarpus_tetragonolobus Psophocarpus~~ tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QYI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Dolichos_tetragonolobus Dolichos tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QYI FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://pdbe.org/2qyi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2qyi" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Bovin]]
-	[[Category: ~~Psophocarpus~~ tetragonolobus]]	+	[[Category: Dolichos tetragonolobus]]
	[[Category: Dasgupta, J]]		[[Category: Dasgupta, J]]
	[[Category: Dattagupta, J K]]		[[Category: Dattagupta, J K]]

OCA at 19:45, 24 December 2014

OCA — Wed, 24 Dec 2014 19:45:10 GMT

←Older revision		Revision as of 19:45, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QYI FirstGlance]. <br>		<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QYI FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ICW3_PSOTE ICW3_PSOTE]] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Psophocarpus tetragonolobus]]		[[Category: Psophocarpus tetragonolobus]]
-	[[Category: Dasgupta, J.]]	+	[[Category: Dasgupta, J]]
-	[[Category: Dattagupta, J K.]]	+	[[Category: Dattagupta, J K]]
-	[[Category: Khamrui, S.]]	+	[[Category: Khamrui, S]]
-	[[Category: Sen, U.]]	+	[[Category: Sen, U]]
	[[Category: Bovine trypsin]]		[[Category: Bovine trypsin]]
	[[Category: Complex]]		[[Category: Complex]]

OCA at 06:21, 29 September 2014

OCA — Mon, 29 Sep 2014 06:21:00 GMT

←Older revision		Revision as of 06:21, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2qyi.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of a binary complex between an engineered trypsin inhibitor and Bovine trypsin==
		+	<StructureSection load='2qyi' size='340' side='right' caption='[[2qyi]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2qyi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QYI FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eyl\|1eyl]], [[1fmz\|1fmz]], [[1xg6\|1xg6]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qyi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qyi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2qyi RCSB], [http://www.ebi.ac.uk/pdbsum/2qyi PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qy/2qyi_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Change in specificity, caused by the mutations at P1 site, of the serine protease inhibitors of different families is reported in the literature, but Kunitz (STI) family inhibitors are almost unexplored in this regard. In this paper, we present the crystal structure of a P1 variant of winged bean chymotrypsin inhibitor (WCI) belonging to Kunitz (STI) family, supplemented by biochemical, phylogenetic and docking studies on the mutant. A single mutation (Leu-->Arg) at P1 converted WCI to a strong inhibitor of trypsin with an association constant of 4.8x10(10) M(-1) which is comparable to other potent trypsin inhibitors of the family. The crystal structure (2.15 A) of this mutant (L65R) shows that its reactive site loop conformation deviates from that of WCI and adopts a structure similar to that of Erythrina caffra trypsin inhibitor (ETI) belonging to the same family. Mutation induced structural changes have also been propagated in a concerted manner to the neighboring conserved scaffolding residue Asn14, such that the side chain of this residue took an orientation similar to that of ETI and optimized the hydrogen bonds with the loop residues. While docking studies provide information about the accommodation of non-specific residues in the active site groove of trypsin, the basis of the directional alteration of the reactive site loop conformation has been understood through sequence analysis and related phylogenetic studies.

-	~~{{STRUCTURE_2qyi\| PDB=2qyi \| SCENE= }}~~	+	Single mutation at P1 of a chymotrypsin inhibitor changes it to a trypsin inhibitor: X-ray structural (2.15 A) and biochemical basis.,Khamrui S, Dasgupta J, Dattagupta JK, Sen U Biochim Biophys Acta. 2005 Aug 31;1752(1):65-72. PMID:16081330<ref>PMID:16081330</ref>

-	~~===Crystal structure~~ of ~~a binary complex between an engineered trypsin inhibitor and Bovine trypsin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16081330}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2qyi]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus] and [http://en~~.~~wikipedia.org~~/~~wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA].~~	+

	==See Also==		==See Also==
	*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]		*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016081330<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Psophocarpus tetragonolobus]]		[[Category: Psophocarpus tetragonolobus]]

OCA at 08:31, 20 October 2012

OCA — Sat, 20 Oct 2012 08:31:59 GMT

←Older revision		Revision as of 08:31, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2qyi]] is a 4 chain structure ~~of [[Chymotrypsin Inhibitor]] and [[Trypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA].	+	[[2qyi]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA].

	==See Also==		==See Also==

OCA at 19:49, 25 July 2012

OCA — Wed, 25 Jul 2012 19:49:25 GMT

←Older revision		Revision as of 19:49, 25 July 2012
Line 1:		Line 1:
	[[Image:2qyi.png\|left\|200px]]		[[Image:2qyi.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2qyi", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2qyi\| PDB=2qyi \| SCENE= }}		{{STRUCTURE_2qyi\| PDB=2qyi \| SCENE= }}

	===Crystal structure of a binary complex between an engineered trypsin inhibitor and Bovine trypsin===		===Crystal structure of a binary complex between an engineered trypsin inhibitor and Bovine trypsin===

		+	{{ABSTRACT_PUBMED_16081330}}

	==About this Structure==		==About this Structure==
-	[[2qyi]] is a 4 chain structure of [[Chymotrypsin Inhibitor]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA].	+	[[2qyi]] is a 4 chain structure of [[Chymotrypsin Inhibitor]] and [[Trypsin]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QYI OCA].

	==See Also==		==See Also==
-	*[[Chymotrypsin Inhibitor]]	+	*[[Chymotrypsin Inhibitor\|Chymotrypsin Inhibitor]]
		+	*[[Trypsin\|Trypsin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16081330~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016081330</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Psophocarpus tetragonolobus]]		[[Category: Psophocarpus tetragonolobus]]
Line 27:		Line 23:
	[[Category: Sen, U.]]		[[Category: Sen, U.]]
	[[Category: Bovine trypsin]]		[[Category: Bovine trypsin]]
-	[[Category: Calcium]]
	[[Category: Complex]]		[[Category: Complex]]
	[[Category: Digestion]]		[[Category: Digestion]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-	[[Category: Hydrolase/hydrolase inhibitor complex]]	+	[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: P1 mutant]]		[[Category: P1 mutant]]

OCA: Protected "2qyi" [edit=sysop:move=sysop]

OCA — Sun, 26 Dec 2010 23:39:20 GMT

Protected "2qyi" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:39, 26 December 2010