2r2g - Revision history

OCA at 11:44, 30 August 2023

OCA — Wed, 30 Aug 2023 11:44:23 GMT

←Older revision		Revision as of 11:44, 30 August 2023
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	<StructureSection load='2r2g' size='340' side='right'caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2r2g' size='340' side='right'caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ociba Ociba~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R2G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ocimum_basilicum Ocimum basilicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R2G FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EMF:ETHYL+(1S,2S)~~-~~2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>~~, ~~<scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EMF:ETHYL+(1S,2S)-2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">EGS1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 OCIBA])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [https://pdbe.org/2r2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [https://www.ebi.ac.uk/pdbsum/2r2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r2g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [https://pdbe.org/2r2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [https://www.ebi.ac.uk/pdbsum/2r2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r2g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/EGS1_OCIBA EGS1_OCIBA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ociba~~]]	+	[[Category: Ocimum basilicum]]
-	[[Category: Bowman~~, M E~~]]	+	[[Category: Bowman ME]]
-	[[Category: Louie~~, G V~~]]	+	[[Category: Louie GV]]
-	[[Category: Noel~~, J P]]~~	+	[[Category: Noel JP]]
-	~~[[Category: Eugenol]]~~	+
-	~~[[Category: Phenylpropene]]~~	+
-	~~[[Category: Pip reductase]]~~	+
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Short-chain dehydrogenase/reductase~~]]	+

OCA at 04:12, 2 July 2021

OCA — Fri, 02 Jul 2021 04:12:05 GMT

←Older revision		Revision as of 04:12, 2 July 2021
Line 1:		Line 1:

	==Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF==		==Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF==
-	<StructureSection load='2r2g' size='340' side='right' caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='2r2g' size='340' side='right'caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ociba Ociba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2R2G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ociba Ociba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R2G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMF:ETHYL+(1S,2S)-2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EMF:ETHYL+(1S,2S)-2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 OCIBA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 OCIBA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [~~http~~://pdbe.org/2r2g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2r2g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2r2g ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [https://pdbe.org/2r2g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [https://www.ebi.ac.uk/pdbsum/2r2g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r2g ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/2r2g_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/2r2g_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Ociba]]		[[Category: Ociba]]
	[[Category: Bowman, M E]]		[[Category: Bowman, M E]]

OCA at 07:41, 25 October 2017

OCA — Wed, 25 Oct 2017 07:41:16 GMT

←Older revision		Revision as of 07:41, 25 October 2017
Line 1:		Line 1:
		+
	==Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF==		==Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF==
	<StructureSection load='2r2g' size='340' side='right' caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2r2g' size='340' side='right' caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 OCIBA])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 OCIBA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [http://pdbe.org/2r2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [http://www.ebi.ac.uk/pdbsum/2r2g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [http://pdbe.org/2r2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [http://www.ebi.ac.uk/pdbsum/2r2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2r2g ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r2g ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:36, 11 September 2015

OCA — Fri, 11 Sep 2015 10:36:00 GMT

←Older revision		Revision as of 10:36, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='2r2g' size='340' side='right' caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2r2g' size='340' side='right' caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Ocimum_basilicum Ocimum basilicum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2R2G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ociba Ociba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2R2G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMF:ETHYL+(1S,2S)-2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMF:ETHYL+(1S,2S)-2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 ~~Ocimum basilicum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 OCIBA])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [http://www.ebi.ac.uk/pdbsum/2r2g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [http://pdbe.org/2r2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [http://www.ebi.ac.uk/pdbsum/2r2g PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2r2g" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ocimum basilicum~~]]	+	[[Category: Ociba]]
	[[Category: Bowman, M E]]		[[Category: Bowman, M E]]
	[[Category: Louie, G V]]		[[Category: Louie, G V]]

OCA at 15:18, 25 December 2014

OCA — Thu, 25 Dec 2014 15:18:08 GMT

←Older revision		Revision as of 15:18, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ocimum_basilicum Ocimum basilicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2R2G FirstGlance]. <br>		<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ocimum_basilicum Ocimum basilicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2R2G FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMF:ETHYL+(1S,2S)-2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMF:ETHYL+(1S,2S)-2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 Ocimum basilicum])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 Ocimum basilicum])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [http://www.ebi.ac.uk/pdbsum/2r2g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [http://www.ebi.ac.uk/pdbsum/2r2g PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Ocimum basilicum]]		[[Category: Ocimum basilicum]]
-	[[Category: Bowman, M E.]]	+	[[Category: Bowman, M E]]
-	[[Category: Louie, G V.]]	+	[[Category: Louie, G V]]
-	[[Category: Noel, J P.]]	+	[[Category: Noel, J P]]
	[[Category: Eugenol]]		[[Category: Eugenol]]
	[[Category: Phenylpropene]]		[[Category: Phenylpropene]]

OCA at 18:08, 30 September 2014

OCA — Tue, 30 Sep 2014 18:08:49 GMT

←Older revision		Revision as of 18:08, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2r2g.~~png~~\|~~left~~\|200px]]	+	==Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF==
		+	<StructureSection load='2r2g' size='340' side='right' caption='[[2r2g]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2r2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ocimum_basilicum Ocimum basilicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2R2G FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EMF:ETHYL+(1S,2S)-2-(4-HYDROXY-3-METHOXYPHENYL)CYCLOPROPANECARBOXYLATE'>EMF</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2qw8\|2qw8]], [[2qx7\|2qx7]], [[2qys\|2qys]], [[2qz2\|2qz2]], [[2qzz\|2qzz]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EGS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39350 Ocimum basilicum])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r2g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2r2g RCSB], [http://www.ebi.ac.uk/pdbsum/2r2g PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/2r2g_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phenylpropenes, a large group of plant volatile compounds that serve in multiple roles in defense and pollinator attraction, contain a propenyl side chain. Eugenol synthase (EGS) catalyzes the reductive displacement of acetate from the propenyl side chain of the substrate coniferyl acetate to produce the allyl-phenylpropene eugenol. We report here the structure determination of EGS from basil (Ocimum basilicum) by protein x-ray crystallography. EGS is structurally related to the short-chain dehydrogenase/reductases (SDRs), and in particular, enzymes in the isoflavone-reductase-like subfamily. The structure of a ternary complex of EGS bound to the cofactor NADP(H) and a mixed competitive inhibitor EMDF ((7S,8S)-ethyl (7,8-methylene)-dihydroferulate) provides a detailed view of the binding interactions within the EGS active site and a starting point for mutagenic examination of the unusual reductive mechanism of EGS. The key interactions between EMDF and the EGS-holoenzyme include stacking of the phenyl ring of EMDF against the cofactor's nicotinamide ring and a water-mediated hydrogen-bonding interaction between the EMDF 4-hydroxy group and the side-chain amino moiety of a conserved lysine residue, Lys132. The C4 carbon of nicotinamide resides immediately adjacent to the site of hydride addition, the C7 carbon of cinnamyl acetate substrates. The inhibitor-bound EGS structure suggests a two-step reaction mechanism involving the formation of a quinone-methide prior to reduction. The formation of this intermediate is promoted by a hydrogen-bonding network that favors deprotonation of the substrate's 4-hydroxyl group and disfavors binding of the acetate moiety, akin to a push-pull catalytic mechanism. Notably, the catalytic involvement in EGS of the conserved Lys132 in preparing the phenolic substrate for quinone methide formation through the proton-relay network appears to be an adaptation of the analogous role in hydrogen bonding played by the equivalent lysine residue in other enzymes of the SDR family.

-	~~{{STRUCTURE_2r2g\| PDB=2r2g \| SCENE= }}~~	+	Structure and reaction mechanism of basil eugenol synthase.,Louie GV, Baiga TJ, Bowman ME, Koeduka T, Taylor JH, Spassova SM, Pichersky E, Noel JP PLoS ONE. 2007 Oct 3;2(10):e993. PMID:17912370<ref>PMID:17912370</ref>

-	~~===Structure~~ of ~~Eugenol Synthase from Ocimum basilicum complexed with EMDF===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17912370}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2r2g]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Ocimum_basilicum Ocimum basilicum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017912370<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Ocimum basilicum]]		[[Category: Ocimum basilicum]]
	[[Category: Bowman, M E.]]		[[Category: Bowman, M E.]]

OCA: Protected "2r2g" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 17:56:43 GMT

Protected "2r2g" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:56, 7 January 2013

OCA at 17:56, 7 January 2013

OCA — Mon, 07 Jan 2013 17:56:40 GMT

←Older revision		Revision as of 17:56, 7 January 2013
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-	{{Seed}}
	[[Image:2r2g.png\|left\|200px]]		[[Image:2r2g.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2r2g", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2r2g\| PDB=2r2g \| SCENE= }}		{{STRUCTURE_2r2g\| PDB=2r2g \| SCENE= }}

	===Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF===		===Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17912370}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17912370 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_17912370}}		{{ABSTRACT_PUBMED_17912370}}

	==About this Structure==		==About this Structure==
-	~~2R2G~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Ocimum_basilicum Ocimum basilicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA].	+	[[2r2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ocimum_basilicum Ocimum basilicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17912370~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017912370</ref><references group="xtra"/>
	[[Category: Ocimum basilicum]]		[[Category: Ocimum basilicum]]
	[[Category: Bowman, M E.]]		[[Category: Bowman, M E.]]
Line 33:		Line 21:
	[[Category: Plant protein]]		[[Category: Plant protein]]
	[[Category: Short-chain dehydrogenase/reductase]]		[[Category: Short-chain dehydrogenase/reductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:02:16 2009''

OCA at 18:02, 17 February 2009

OCA — Tue, 17 Feb 2009 18:02:17 GMT

←Older revision		Revision as of 18:02, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2R2G is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Ocimum_basilicum Ocimum basilicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA].	+	2R2G is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Ocimum_basilicum Ocimum basilicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R2G OCA].

	==Reference==		==Reference==
-	~~Structure and reaction mechanism of basil eugenol synthase., Louie GV, Baiga TJ, Bowman ME, Koeduka T, Taylor JH, Spassova SM, Pichersky E, Noel JP, PLoS ONE. 2007 Oct 3;2(10):e993.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/17912370 17912370]~~	+	<ref group="xtra">PMID:17912370</ref><references group="xtra"/>
	[[Category: Ocimum basilicum]]		[[Category: Ocimum basilicum]]
-	[[Category: Single protein]]
	[[Category: Bowman, M E.]]		[[Category: Bowman, M E.]]
	[[Category: Louie, G V.]]		[[Category: Louie, G V.]]
Line 35:		Line 34:
	[[Category: Short-chain dehydrogenase/reductase]]		[[Category: Short-chain dehydrogenase/reductase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 03~~:56:~~17 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:02:16 2009''

OCA at 00:56, 29 July 2008

OCA — Tue, 29 Jul 2008 00:56:21 GMT

←Older revision		Revision as of 00:56, 29 July 2008
Line 1:		Line 1:
-	[[Image:2r2g.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2r2g.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2r2g\| PDB=2r2g \| SCENE= }}		{{STRUCTURE_2r2g\| PDB=2r2g \| SCENE= }}

-	~~'''~~Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF~~'''~~	+	===Structure of Eugenol Synthase from Ocimum basilicum complexed with EMDF===


-	~~==Overview==~~	+	<!--
-	~~Phenylpropenes~~, ~~a large group of plant volatile compounds that serve in multiple roles in defense and pollinator attraction~~, ~~contain a propenyl side chain. Eugenol synthase (EGS) catalyzes the reductive displacement of acetate from the propenyl side chain of~~ the ~~substrate coniferyl acetate~~ to ~~produce~~ the ~~allyl-phenylpropene eugenol. We report here the structure determination of EGS from basil~~ (~~Ocimum basilicum) by protein x-ray crystallography~~. ~~EGS is structurally related to the short-chain dehydrogenase/reductases (SDRs), and in particular, enzymes in the isoflavone-reductase-like subfamily~~. ~~The structure of a ternary complex of EGS bound to the cofactor NADP(H~~) ~~and a mixed competitive inhibitor EMDF ((7S~~,~~8S)-ethyl (7,8-methylene)-dihydroferulate) provides a detailed view of~~ the ~~binding interactions within the EGS active site and a starting point for mutagenic examination of the unusual reductive mechanism of EGS~~. ~~The key interactions between EMDF and the EGS~~-~~holoenzyme include stacking of the phenyl ring of EMDF against the cofactor's nicotinamide ring and a water~~-mediated hydrogen-bonding interaction between the EMDF 4-hydroxy group and the side-chain amino moiety of a conserved lysine residue, Lys132. The C4 carbon of nicotinamide resides immediately adjacent to the site of hydride addition, the C7 carbon of cinnamyl acetate substrates. The inhibitor-bound EGS structure suggests a two-step reaction mechanism involving the formation of a quinone-methide prior to reduction. The formation of this intermediate is promoted by a hydrogen-bonding network that favors deprotonation of the substrate's 4-hydroxyl group and disfavors binding of the acetate moiety, akin to a push-pull catalytic mechanism. Notably, the catalytic involvement in EGS of the conserved Lys132 in preparing the phenolic substrate for quinone methide formation through the proton-relay network appears to be an adaptation of the analogous role in hydrogen bonding played by the equivalent lysine residue in other enzymes of the SDR family.	+	The line below this paragraph, {{ABSTRACT_PUBMED_17912370}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 17912370 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_17912370}}

	==About this Structure==		==About this Structure==
Line 30:		Line 34:
	[[Category: Plant protein]]		[[Category: Plant protein]]
	[[Category: Short-chain dehydrogenase/reductase]]		[[Category: Short-chain dehydrogenase/reductase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 16~~:07:28 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:56:17 2008''