2rcj - Revision history

OCA at 09:21, 21 February 2024

OCA — Wed, 21 Feb 2024 09:21:28 GMT

←Older revision		Revision as of 09:21, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='2rcj' size='340' side='right'caption='[[2rcj]]' scene=''>		<StructureSection load='2rcj' size='340' side='right'caption='[[2rcj]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[1iga\|1iga]], [[2esg\|2esg]], [[2qtj\|2qtj]]~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray solution scattering</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [~~http~~://pdbe.org/2rcj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rcj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rcj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [https://pdbe.org/2rcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [https://www.ebi.ac.uk/pdbsum/2rcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rcj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6PYX1_HUMAN Q6PYX1_HUMAN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rcj ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rcj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The pentameric 71-domain structure of human and mouse immunoglobulin M (IgM) was investigated by synchrotron X-ray solution scattering and molecular graphics modelling. The radii of gyration RG of human IgM Quaife and its Fc5, IgM-S, Fab'2 and Fab fragments were determined as 12.2 nm, 6.1 nm, 6.1 nm, 4.9 nm and 2.9 nm in that order. The RG values were similar for mouse IgM P8 and its Fab'2 and Fab fragments, despite the presence of an additional carbohydrate site. The IgM scattering curves, to a nominal resolution of 5 nm, were compared with molecular graphics models based on published crystallographic alpha-carbon co-ordinates for the Fab and Fc structures of IgG. Good curve fits for Fab were obtained based on the crystal structure of Fab from IgG. A good curve fit was obtained for Fab'2, if the two Fab arms were positioned close together at their contact with the C mu 2 domains. The addition of the Fc fragment close to the C mu 2 domains of this Fab'2 model, to give a planar structure, accounted for the scattering curve of IgM-S. The Fc5 fragment was best modelled by a ring of five Fc monomers, constrained by packing considerations and disulphide bridge formation. A position for the J chain between two C mu 4 domains rather than at the centre of Fc5 was preferred. The intact IgM structure was best modelled using a planar arrangement of these Fab'2 and Fc5 models, with the side-to-side displacement of the Fab'2 arms in the plane of the IgM structure. All these models were consistent with hydrodynamic simulations of sedimentation data. The solution structure of IgM can therefore be reproduced quantitatively in terms of crystallographic structures for the fragments of IgG. Putative Clq binding sites have been identified on the C mu 3 domain. These would become accessible for interaction with Clq when the Fab'2 arms move out of the plane of the Fc5 disc in IgM, that is, a steric mechanism exposing pre-existing Clq sites. Comparison with a solution structure for Clq by neutron scattering shows that two or more of the six globular Clq heads in the hexameric head-and-stalk structure are readily able to make contacts with the putative Clq sites in the C mu 3 domains of free IgM if if the Clq arm-axis angle in solution is reduced from 40 degrees-45 degrees to 28 degrees. This could be the trigger for Cl activation.
-
-	Solution structure of human and mouse immunoglobulin M by synchrotron X-ray scattering and molecular graphics modelling. A possible mechanism for complement activation.,Perkins SJ, Nealis AS, Sutton BJ, Feinstein A J Mol Biol. 1991 Oct 20;221(4):1345-66. PMID:1942055<ref>PMID:1942055</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2rcj" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[IgA\|IgA]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Feinstein, A]]	+	[[Category: Feinstein A]]
-	[[Category: Nealis~~, A S~~]]	+	[[Category: Nealis AS]]
-	[[Category: Perkins~~, S J~~]]	+	[[Category: Perkins SJ]]
-	[[Category: Sutton~~, B J]]~~	+	[[Category: Sutton BJ]]
-	~~[[Category: Constrained modelling]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunology]]~~	+
-	~~[[Category: Polymeric antibody~~]]	+

OCA at 11:18, 23 September 2020

OCA — Wed, 23 Sep 2020 11:18:12 GMT

←Older revision		Revision as of 11:18, 23 September 2020
Line 3:		Line 3:
	<StructureSection load='2rcj' size='340' side='right'caption='[[2rcj]]' scene=''>		<StructureSection load='2rcj' size='340' side='right'caption='[[2rcj]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iga\|1iga]], [[2esg\|2esg]], [[2qtj\|2qtj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iga\|1iga]], [[2esg\|2esg]], [[2qtj\|2qtj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [http://pdbe.org/2rcj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [http://www.ebi.ac.uk/pdbsum/2rcj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rcj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [http://pdbe.org/2rcj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [http://www.ebi.ac.uk/pdbsum/2rcj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rcj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 41:		Line 41:
	[[Category: Constrained modelling]]		[[Category: Constrained modelling]]
	[[Category: Immune system]]		[[Category: Immune system]]
-	[[Category: Immunoglobulin m]]
	[[Category: Immunology]]		[[Category: Immunology]]
	[[Category: Polymeric antibody]]		[[Category: Polymeric antibody]]
-	[[Category: X-ray solution scattering]]

OCA at 07:52, 31 July 2019

OCA — Wed, 31 Jul 2019 07:52:02 GMT

←Older revision		Revision as of 07:52, 31 July 2019
Line 1:		Line 1:

	==Solution structure of human Immunoglobulin M==		==Solution structure of human Immunoglobulin M==
-	<StructureSection load='2rcj' size='340' side='right' caption='[[2rcj]]' scene=''>	+	<StructureSection load='2rcj' size='340' side='right'caption='[[2rcj]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/2rcj_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/2rcj_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
	[[Category: Feinstein, A]]		[[Category: Feinstein, A]]
	[[Category: Nealis, A S]]		[[Category: Nealis, A S]]

OCA at 17:26, 11 August 2016

OCA — Thu, 11 Aug 2016 17:26:00 GMT

←Older revision		Revision as of 17:26, 11 August 2016
Line 1:		Line 1:
		+
	==Solution structure of human Immunoglobulin M==		==Solution structure of human Immunoglobulin M==
	<StructureSection load='2rcj' size='340' side='right' caption='[[2rcj]]' scene=''>		<StructureSection load='2rcj' size='340' side='right' caption='[[2rcj]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iga\|1iga]], [[2esg\|2esg]], [[2qtj\|2qtj]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iga\|1iga]], [[2esg\|2esg]], [[2qtj\|2qtj]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [http://www.ebi.ac.uk/pdbsum/2rcj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [http://pdbe.org/2rcj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [http://www.ebi.ac.uk/pdbsum/2rcj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rcj ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rcj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2rcj" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 18:29, 19 January 2015

OCA — Mon, 19 Jan 2015 18:29:07 GMT

←Older revision		Revision as of 18:29, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iga\|1iga]], [[2esg\|2esg]], [[2qtj\|2qtj]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iga\|1iga]], [[2esg\|2esg]], [[2qtj\|2qtj]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [http://www.ebi.ac.uk/pdbsum/2rcj PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [http://www.ebi.ac.uk/pdbsum/2rcj PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Feinstein, A.]]	+	[[Category: Feinstein, A]]
-	[[Category: Nealis, A S.]]	+	[[Category: Nealis, A S]]
-	[[Category: Perkins, S J.]]	+	[[Category: Perkins, S J]]
-	[[Category: Sutton, B J.]]	+	[[Category: Sutton, B J]]
	[[Category: Constrained modelling]]		[[Category: Constrained modelling]]
	[[Category: Immune system]]		[[Category: Immune system]]

OCA at 20:15, 30 September 2014

OCA — Tue, 30 Sep 2014 20:15:17 GMT

←Older revision		Revision as of 20:15, 30 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2rcj\| PDB~~=2rcj \| ~~SCENE~~= }}	+	==Solution structure of human Immunoglobulin M==
-	===~~Solution~~ structure of ~~human Immunoglobulin M~~===	+	<StructureSection load='2rcj' size='340' side='right' caption='[[2rcj]]' scene=''>
-	~~{{ABSTRACT_PUBMED_1942055}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1iga\|1iga]], [[2esg\|2esg]], [[2qtj\|2qtj]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [http://www.ebi.ac.uk/pdbsum/2rcj PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/2rcj_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The pentameric 71-domain structure of human and mouse immunoglobulin M (IgM) was investigated by synchrotron X-ray solution scattering and molecular graphics modelling. The radii of gyration RG of human IgM Quaife and its Fc5, IgM-S, Fab'2 and Fab fragments were determined as 12.2 nm, 6.1 nm, 6.1 nm, 4.9 nm and 2.9 nm in that order. The RG values were similar for mouse IgM P8 and its Fab'2 and Fab fragments, despite the presence of an additional carbohydrate site. The IgM scattering curves, to a nominal resolution of 5 nm, were compared with molecular graphics models based on published crystallographic alpha-carbon co-ordinates for the Fab and Fc structures of IgG. Good curve fits for Fab were obtained based on the crystal structure of Fab from IgG. A good curve fit was obtained for Fab'2, if the two Fab arms were positioned close together at their contact with the C mu 2 domains. The addition of the Fc fragment close to the C mu 2 domains of this Fab'2 model, to give a planar structure, accounted for the scattering curve of IgM-S. The Fc5 fragment was best modelled by a ring of five Fc monomers, constrained by packing considerations and disulphide bridge formation. A position for the J chain between two C mu 4 domains rather than at the centre of Fc5 was preferred. The intact IgM structure was best modelled using a planar arrangement of these Fab'2 and Fc5 models, with the side-to-side displacement of the Fab'2 arms in the plane of the IgM structure. All these models were consistent with hydrodynamic simulations of sedimentation data. The solution structure of IgM can therefore be reproduced quantitatively in terms of crystallographic structures for the fragments of IgG. Putative Clq binding sites have been identified on the C mu 3 domain. These would become accessible for interaction with Clq when the Fab'2 arms move out of the plane of the Fc5 disc in IgM, that is, a steric mechanism exposing pre-existing Clq sites. Comparison with a solution structure for Clq by neutron scattering shows that two or more of the six globular Clq heads in the hexameric head-and-stalk structure are readily able to make contacts with the putative Clq sites in the C mu 3 domains of free IgM if if the Clq arm-axis angle in solution is reduced from 40 degrees-45 degrees to 28 degrees. This could be the trigger for Cl activation.

-	~~==About this Structure==~~	+	Solution structure of human and mouse immunoglobulin M by synchrotron X-ray scattering and molecular graphics modelling. A possible mechanism for complement activation.,Perkins SJ, Nealis AS, Sutton BJ, Feinstein A J Mol Biol. 1991 Oct 20;221(4):1345-66. PMID:1942055<ref>PMID:1942055</ref>
-	~~[[2rcj]] is a 21 chain~~ structure ~~with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2RCJ OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[IgA\|IgA]]		*[[IgA\|IgA]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:001942055<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Feinstein, A.]]		[[Category: Feinstein, A.]]

OCA at 11:08, 11 March 2013

OCA — Mon, 11 Mar 2013 11:08:51 GMT

←Older revision		Revision as of 11:08, 11 March 2013
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-	[[Image:2rcj.png\|left\|200px]]
-
	{{STRUCTURE_2rcj\| PDB=2rcj \| SCENE= }}		{{STRUCTURE_2rcj\| PDB=2rcj \| SCENE= }}
-
	===Solution structure of human Immunoglobulin M===		===Solution structure of human Immunoglobulin M===
-
	{{ABSTRACT_PUBMED_1942055}}		{{ABSTRACT_PUBMED_1942055}}

OCA at 13:10, 30 January 2013

OCA — Wed, 30 Jan 2013 13:10:44 GMT

←Older revision		Revision as of 13:10, 30 January 2013
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	[[Image:2rcj.png\|left\|200px]]		[[Image:2rcj.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2rcj", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2rcj\| PDB=2rcj \| SCENE= }}		{{STRUCTURE_2rcj\| PDB=2rcj \| SCENE= }}

	===Solution structure of human Immunoglobulin M===		===Solution structure of human Immunoglobulin M===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_1942055}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 1942055 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_1942055}}		{{ABSTRACT_PUBMED_1942055}}

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	==See Also==		==See Also==
-	*[[IgA]]	+	*[[IgA\|IgA]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~1942055</ref><ref group="xtra">PMID:18178841~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:001942055</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Feinstein, A.]]		[[Category: Feinstein, A.]]

OCA: Protected "2rcj" [edit=sysop:move=sysop]

OCA — Tue, 15 Mar 2011 00:21:46 GMT

Protected "2rcj" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:21, 15 March 2011

OCA at 00:21, 15 March 2011

OCA — Tue, 15 Mar 2011 00:21:45 GMT

←Older revision		Revision as of 00:21, 15 March 2011
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-	{{Seed}}
	[[Image:2rcj.png\|left\|200px]]		[[Image:2rcj.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~2RCJ~~ is a 21 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA].	+	[[2rcj]] is a 21 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA].
		+
		+	==See Also==
		+	*[[IgA]]

	==Reference==		==Reference==
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	[[Category: Polymeric antibody]]		[[Category: Polymeric antibody]]
	[[Category: X-ray solution scattering]]		[[Category: X-ray solution scattering]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 7 09:59:20 2010''