2reh - Revision history

OCA at 09:03, 25 October 2023

OCA — Wed, 25 Oct 2023 09:03:52 GMT

←Older revision		Revision as of 09:03, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2REH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2REH FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [https://pdbe.org/2reh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [https://www.ebi.ac.uk/pdbsum/2reh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2reh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [https://pdbe.org/2reh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [https://www.ebi.ac.uk/pdbsum/2reh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2reh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NAO_FUSOX NAO_FUSOX]] Catalyzes the oxidative denitrification of neutral nitroalkanes, including 3-nitro-2-pentanol, 1-nitropropane, 2-nitropropane, nitroethane and nitrocyclohexane, and may thereby protect the organism against toxic compounds. Has no detectable acyl-CoA dehydrogenase activity.<ref>PMID:11867731</ref> <ref>PMID:22538</ref> <ref>PMID:16430210</ref>	+	[https://www.uniprot.org/uniprot/NAO_FUSOX NAO_FUSOX] Catalyzes the oxidative denitrification of neutral nitroalkanes, including 3-nitro-2-pentanol, 1-nitropropane, 2-nitropropane, nitroethane and nitrocyclohexane, and may thereby protect the organism against toxic compounds. Has no detectable acyl-CoA dehydrogenase activity.<ref>PMID:11867731</ref> <ref>PMID:22538</ref> <ref>PMID:16430210</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 35:
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Nitroalkane oxidase]]~~	+	[[Category: Bozinovski DM]]
-	[[Category: Bozinovski~~, D M~~]]	+	[[Category: Fitzpatrick PF]]
-	[[Category: Fitzpatrick~~, P F~~]]	+	[[Category: Heroux A]]
-	[[Category: Heroux, A]]	+	[[Category: Orville AM]]
-	[[Category: Orville~~, A M~~]]	+	[[Category: Shaw PG]]
-	[[Category: Shaw~~, P G~~]]	+	[[Category: Valley MP]]
-	[[Category: Valley~~, M P]]~~	+
-	~~[[Category: Flavoenzyme]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Nitroalkanefad]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 07:09, 10 November 2021

OCA — Wed, 10 Nov 2021 07:09:07 GMT

←Older revision		Revision as of 07:09, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='2reh' size='340' side='right'caption='[[2reh]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2reh' size='340' side='right'caption='[[2reh]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2REH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2REH FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [~~http~~://pdbe.org/2reh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2reh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2reh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [https://pdbe.org/2reh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [https://www.ebi.ac.uk/pdbsum/2reh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2reh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NAO_FUSOX NAO_FUSOX]] Catalyzes the oxidative denitrification of neutral nitroalkanes, including 3-nitro-2-pentanol, 1-nitropropane, 2-nitropropane, nitroethane and nitrocyclohexane, and may thereby protect the organism against toxic compounds. Has no detectable acyl-CoA dehydrogenase activity.<ref>PMID:11867731</ref> <ref>PMID:22538</ref> <ref>PMID:16430210</ref>	+	[[https://www.uniprot.org/uniprot/NAO_FUSOX NAO_FUSOX]] Catalyzes the oxidative denitrification of neutral nitroalkanes, including 3-nitro-2-pentanol, 1-nitropropane, 2-nitropropane, nitroethane and nitrocyclohexane, and may thereby protect the organism against toxic compounds. Has no detectable acyl-CoA dehydrogenase activity.<ref>PMID:11867731</ref> <ref>PMID:22538</ref> <ref>PMID:16430210</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:11, 10 June 2020

OCA — Wed, 10 Jun 2020 07:11:39 GMT

←Older revision		Revision as of 07:11, 10 June 2020
Line 1:		Line 1:

	==Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase==		==Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase==
-	<StructureSection load='2reh' size='340' side='right' caption='[[2reh]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='2reh' size='340' side='right'caption='[[2reh]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2REH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2REH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://pdbe.org/2reh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2reh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://pdbe.org/2reh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2reh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
		+	[[Category: Large Structures]]
	[[Category: Nitroalkane oxidase]]		[[Category: Nitroalkane oxidase]]
	[[Category: Bozinovski, D M]]		[[Category: Bozinovski, D M]]

OCA at 11:32, 5 September 2018

OCA — Wed, 05 Sep 2018 11:32:58 GMT

←Older revision		Revision as of 11:32, 5 September 2018
Line 1:		Line 1:
		+
	==Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase==		==Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase==
	<StructureSection load='2reh' size='340' side='right' caption='[[2reh]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='2reh' size='340' side='right' caption='[[2reh]], [[Resolution\|resolution]] 2.40Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://pdbe.org/2reh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://pdbe.org/2reh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2reh ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/2reh_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/2reh_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 12:23, 9 February 2016

OCA — Tue, 09 Feb 2016 12:23:20 GMT

←Older revision		Revision as of 12:23, 9 February 2016
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://pdbe.org/2reh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NAO_FUSOX NAO_FUSOX]] Catalyzes the oxidative denitrification of neutral nitroalkanes, including 3-nitro-2-pentanol, 1-nitropropane, 2-nitropropane, nitroethane and nitrocyclohexane, and may thereby protect the organism against toxic compounds. Has no detectable acyl-CoA dehydrogenase activity.<ref>PMID:11867731</ref> <ref>PMID:22538</ref> <ref>PMID:16430210</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2reh ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2reh" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:41, 19 January 2015

OCA — Mon, 19 Jan 2015 18:41:32 GMT

←Older revision		Revision as of 18:41, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2REH FirstGlance]. <br>		<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2REH FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
	[[Category: Nitroalkane oxidase]]		[[Category: Nitroalkane oxidase]]
-	[[Category: Bozinovski, D M.]]	+	[[Category: Bozinovski, D M]]
-	[[Category: Fitzpatrick, P F.]]	+	[[Category: Fitzpatrick, P F]]
-	[[Category: Heroux, A.]]	+	[[Category: Heroux, A]]
-	[[Category: Orville, A M.]]	+	[[Category: Orville, A M]]
-	[[Category: Shaw, P G.]]	+	[[Category: Shaw, P G]]
-	[[Category: Valley, M P.]]	+	[[Category: Valley, M P]]
	[[Category: Flavoenzyme]]		[[Category: Flavoenzyme]]
	[[Category: Flavoprotein]]		[[Category: Flavoprotein]]
	[[Category: Nitroalkanefad]]		[[Category: Nitroalkanefad]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 20:21, 30 September 2014

OCA — Tue, 30 Sep 2014 20:21:35 GMT

←Older revision		Revision as of 20:21, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2reh.~~png~~\|~~left~~\|200px]]	+	==Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase==
		+	<StructureSection load='2reh' size='340' side='right' caption='[[2reh]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2reh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2REH FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2c0u\|2c0u]], [[2c12\|2c12]], [[2zaf\|2zaf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2reh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2reh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2reh RCSB], [http://www.ebi.ac.uk/pdbsum/2reh PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/2reh_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The flavoprotein nitroalkane oxidase (NAO) catalyzes the oxidation of primary and secondary nitroalkanes to the corresponding aldehydes and ketones. The enzyme is a homologue of acyl-CoA dehydrogenase. Asp402 in NAO has been proposed to be the active site base responsible for removing the substrate proton in the first catalytic step; structurally it corresponds to the glutamate which acts as the base in medium chain acyl-CoA dehydrogenase. In the active site of NAO, the carboxylate of Asp402 forms an ionic interaction with the side chain of Arg409. The R409K enzyme has now been characterized kinetically and structurally. The mutation results in a decrease in the rate constant for proton abstraction of 100-fold. Analysis of the three-dimensional structure of the R409K enzyme, determined by X-ray crystallography to a resolution of 2.65 A, shows that the critical structural change is an increase in the distance between the carboxylate of Asp402 and the positively charged nitrogen in the side chain of the residue at position 409. The D402E mutation results in a smaller decrease in the rate constant for proton abstraction of 18-fold. The structure of the D402E enzyme, determined at 2.4 A resolution, shows that there is a smaller increase in the distance between Arg409 and the carboxylate at position 402, and the interaction of this residue with Ser276 is perturbed. These results establish the critical importance of the interaction between Asp402 and Arg409 for proton abstraction by nitroalkane oxidase.

-	~~{{STRUCTURE_2reh\| PDB=2reh \| SCENE= }}~~	+	Mechanistic and structural analyses of the roles of Arg409 and Asp402 in the reaction of the flavoprotein nitroalkane oxidase.,Fitzpatrick PF, Bozinovski DM, Heroux A, Shaw PG, Valley MP, Orville AM Biochemistry. 2007 Dec 4;46(48):13800-8. Epub 2007 Nov 10. PMID:17994768<ref>PMID:17994768</ref>

-	~~===Mechanistic and Structural Analyses~~ of the ~~Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17994768}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2reh]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Fusarium_oxysporum Fusarium oxysporum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017994768<~~/~~ref~~><~~ref group="xtra">PMID:016430210</ref><references group="xtra"~~/>	+
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
	[[Category: Nitroalkane oxidase]]		[[Category: Nitroalkane oxidase]]

OCA at 13:21, 30 January 2013

OCA — Wed, 30 Jan 2013 13:21:15 GMT

←Older revision		Revision as of 13:21, 30 January 2013
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	[[Image:2reh.png\|left\|200px]]		[[Image:2reh.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2reh\| PDB=2reh \| SCENE= }}		{{STRUCTURE_2reh\| PDB=2reh \| SCENE= }}

	===Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase===		===Mechanistic and Structural Analyses of the Roles of Arg409 and Asp402 in the Reaction of the Flavoprotein Nitroalkane Oxidase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17994768}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17994768 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17994768}}		{{ABSTRACT_PUBMED_17994768}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17994768~~</ref><ref group="xtra">PMID:~~16430210~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017994768</ref><ref group="xtra">PMID:016430210</ref><references group="xtra"/>
	[[Category: Fusarium oxysporum]]		[[Category: Fusarium oxysporum]]
	[[Category: Nitroalkane oxidase]]		[[Category: Nitroalkane oxidase]]

OCA: Protected "2reh" [edit=sysop:move=sysop]

OCA — Tue, 15 Mar 2011 00:43:51 GMT

Protected "2reh" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:43, 15 March 2011

OCA at 00:43, 15 March 2011

OCA — Tue, 15 Mar 2011 00:43:49 GMT

←Older revision		Revision as of 00:43, 15 March 2011
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2reh.png\|left\|200px]]		[[Image:2reh.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~2REH~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA].	+	[[2reh]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REH OCA].

	==Reference==		==Reference==
Line 36:		Line 35:
	[[Category: Nitroalkanefad]]		[[Category: Nitroalkanefad]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:45:16 2009''