2rfz - Revision history

OCA at 20:54, 20 October 2021

OCA — Wed, 20 Oct 2021 20:54:16 GMT

←Older revision		Revision as of 20:54, 20 October 2021
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	<StructureSection load='2rfz' size='340' side='right'caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2rfz' size='340' side='right'caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_16460 Atcc 16460]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_16460 Atcc 16460]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~CTR~~:~~CELLOTRIOSE~~'>~~CTR~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [~~http~~://pdbe.org/2rfz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rfz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rfz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [https://pdbe.org/2rfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [https://www.ebi.ac.uk/pdbsum/2rfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rfz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 09:44, 25 December 2019

OCA — Wed, 25 Dec 2019 09:44:12 GMT

←Older revision		Revision as of 09:44, 25 December 2019
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	==Crystal structure of cellobiohydrolase from Melanocarpus albomyces complexed with cellotriose==		==Crystal structure of cellobiohydrolase from Melanocarpus albomyces complexed with cellotriose==
-	<StructureSection load='2rfz' size='340' side='right' caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='2rfz' size='340' side='right'caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16460 Atcc 16460]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16460 Atcc 16460]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 16460]]		[[Category: Atcc 16460]]
		+	[[Category: Large Structures]]
	[[Category: Koivula, A]]		[[Category: Koivula, A]]
	[[Category: Parkkinen, T]]		[[Category: Parkkinen, T]]

OCA at 16:27, 15 August 2018

OCA — Wed, 15 Aug 2018 16:27:10 GMT

←Older revision		Revision as of 16:27, 15 August 2018
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	<StructureSection load='2rfz' size='340' side='right' caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2rfz' size='340' side='right' caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Melanocarpus_albomyces Melanocarpus albomyces~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16460 Atcc 16460]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Melanocarpus albomyces~~]]	+	[[Category: Atcc 16460]]
	[[Category: Koivula, A]]		[[Category: Koivula, A]]
	[[Category: Parkkinen, T]]		[[Category: Parkkinen, T]]

OCA at 18:57, 1 August 2018

OCA — Wed, 01 Aug 2018 18:57:55 GMT

←Older revision		Revision as of 18:57, 1 August 2018
Line 1:		Line 1:
		+
	==Crystal structure of cellobiohydrolase from Melanocarpus albomyces complexed with cellotriose==		==Crystal structure of cellobiohydrolase from Melanocarpus albomyces complexed with cellotriose==
	<StructureSection load='2rfz' size='340' side='right' caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2rfz' size='340' side='right' caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [http://pdbe.org/2rfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [http://www.ebi.ac.uk/pdbsum/2rfz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [http://pdbe.org/2rfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [http://www.ebi.ac.uk/pdbsum/2rfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rfz ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/2rfz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/2rfz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 12:38, 8 February 2016

OCA — Mon, 08 Feb 2016 12:38:02 GMT

←Older revision		Revision as of 12:38, 8 February 2016
Line 6:		Line 6:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-~~cellobiosidase~~ Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(non-reducing_end) Cellulose 1,4-beta-cellobiosidase (non-reducing end)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [http://www.ebi.ac.uk/pdbsum/2rfz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [http://pdbe.org/2rfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [http://www.ebi.ac.uk/pdbsum/2rfz PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rfz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2rfz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cellulose 1,4-beta-cellobiosidase]]
	[[Category: Melanocarpus albomyces]]		[[Category: Melanocarpus albomyces]]
	[[Category: Koivula, A]]		[[Category: Koivula, A]]

OCA at 16:18, 19 January 2015

OCA — Mon, 19 Jan 2015 16:18:42 GMT

←Older revision		Revision as of 16:18, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [http://www.ebi.ac.uk/pdbsum/2rfz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [http://www.ebi.ac.uk/pdbsum/2rfz PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Cellulose 1,4-beta-cellobiosidase]]		[[Category: Cellulose 1,4-beta-cellobiosidase]]
	[[Category: Melanocarpus albomyces]]		[[Category: Melanocarpus albomyces]]
-	[[Category: Koivula, A.]]	+	[[Category: Koivula, A]]
-	[[Category: Parkkinen, T.]]	+	[[Category: Parkkinen, T]]
-	[[Category: Rouvinen, J.]]	+	[[Category: Rouvinen, J]]
-	[[Category: Vehmaanper, J.]]	+	[[Category: Vehmaanper, J]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 19:14, 30 September 2014

OCA — Tue, 30 Sep 2014 19:14:07 GMT

←Older revision		Revision as of 19:14, 30 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2rfz.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of cellobiohydrolase from Melanocarpus albomyces complexed with cellotriose==
		+	<StructureSection load='2rfz' size='340' side='right' caption='[[2rfz]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2rfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RFZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTR:CELLOTRIOSE'>CTR</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rfw\|2rfw]], [[2rfy\|2rfy]], [[2rg0\|2rg0]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase Cellulose 1,4-beta-cellobiosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.91 3.2.1.91] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rfz RCSB], [http://www.ebi.ac.uk/pdbsum/2rfz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/2rfz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cellobiohydrolase from Melanocarpus albomyces (Cel7B) is a thermostable, single-module, cellulose-degrading enzyme. It has relatively low catalytic activity under normal temperatures, which allows structural studies of the binding of unmodified substrates to the native enzyme. In this study, we have determined the crystal structure of native Ma Cel7B free and in complex with three different cello-oligomers: cellobiose (Glc(2)), cellotriose (Glc(3)), and cellotetraose (Glc(4)), at high resolution (1.6-2.1 A). In each case, four molecules were found in the asymmetric unit, which provided 12 different complex structures. The overall fold of the enzyme is characteristic of a glycoside hydrolase family 7 cellobiohydrolase, where the loops extending from the core beta-sandwich structure form a long tunnel composed of multiple subsites for the binding of the glycosyl units of a cellulose chain. The catalytic residues at the reducing end of the tunnel are conserved, and the mechanism is expected to be retaining similarly to the other family 7 members. The oligosaccharides in different complex structures occupied different subsite sets, which partly overlapped and ranged from -5 to +2. In four cellotriose and one cellotetraose complex structures, the cello-oligosaccharide also spanned over the cleavage site (-1/+1). There were surprisingly large variations in the amino acid side chain conformations and in the positions of glycosyl units in the different cello-oligomer complexes, particularly at subsites near the catalytic site. However, in each complex structure, all glycosyl residues were in the chair (4C(1)) conformation. Implications in relation to the complex structures with respect to the reaction mechanism are discussed.

-	~~{{STRUCTURE_2rfz\| PDB=2rfz \| SCENE= }}~~	+	Crystal structures of Melanocarpus albomyces cellobiohydrolase Cel7B in complex with cello-oligomers show high flexibility in the substrate binding.,Parkkinen T, Koivula A, Vehmaanpera J, Rouvinen J Protein Sci. 2008 Aug;17(8):1383-94. Epub 2008 May 21. PMID:18499583<ref>PMID:18499583</ref>

-	~~===Crystal structure~~ of ~~cellobiohydrolase from Melanocarpus albomyces complexed with cellotriose===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18499583}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2rfz]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018499583<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Cellulose 1,4-beta-cellobiosidase]]		[[Category: Cellulose 1,4-beta-cellobiosidase]]
	[[Category: Melanocarpus albomyces]]		[[Category: Melanocarpus albomyces]]

OCA: Protected "2rfz" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 19:15:51 GMT

Protected "2rfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:15, 7 January 2013

OCA at 19:15, 7 January 2013

OCA — Mon, 07 Jan 2013 19:15:50 GMT

←Older revision		Revision as of 19:15, 7 January 2013
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-	{{Seed}}
	[[Image:2rfz.png\|left\|200px]]		[[Image:2rfz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2rfz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2rfz\| PDB=2rfz \| SCENE= }}		{{STRUCTURE_2rfz\| PDB=2rfz \| SCENE= }}

	===Crystal structure of cellobiohydrolase from Melanocarpus albomyces complexed with cellotriose===		===Crystal structure of cellobiohydrolase from Melanocarpus albomyces complexed with cellotriose===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18499583}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18499583 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18499583}}		{{ABSTRACT_PUBMED_18499583}}

	==About this Structure==		==About this Structure==
-	~~2RFZ~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA].	+	[[2rfz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18499583~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018499583</ref><references group="xtra"/>
	[[Category: Cellulose 1,4-beta-cellobiosidase]]		[[Category: Cellulose 1,4-beta-cellobiosidase]]
	[[Category: Melanocarpus albomyces]]		[[Category: Melanocarpus albomyces]]
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	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:03:51 2009''

OCA at 20:03, 16 February 2009

OCA — Mon, 16 Feb 2009 20:03:51 GMT

←Older revision		Revision as of 20:03, 16 February 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:2rfz.~~jpg~~\|left\|200px]]	+	[[Image:2rfz.png\|left\|200px]]

	<!--		<!--
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2RFZ is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA].	+	2RFZ is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Melanocarpus_albomyces Melanocarpus albomyces]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFZ OCA].

	==Reference==		==Reference==
-	Crystal structures of Melanocarpus albomyces cellobiohydrolase Cel7B in complex with cello-oligomers show high flexibility in the substrate binding., Parkkinen T, Koivula A, Vehmaanpera J, Rouvinen J, Protein Sci. 2008 Aug;17(8):1383-94. Epub 2008 May 21. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18499583 18499583]~~	+	<ref group="xtra">PMID:18499583</ref><references group="xtra"/>
	[[Category: Cellulose 1,4-beta-cellobiosidase]]		[[Category: Cellulose 1,4-beta-cellobiosidase]]
	[[Category: Melanocarpus albomyces]]		[[Category: Melanocarpus albomyces]]
-	[[Category: Single protein]]
	[[Category: Koivula, A.]]		[[Category: Koivula, A.]]
	[[Category: Parkkinen, T.]]		[[Category: Parkkinen, T.]]
Line 34:		Line 33:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Sep 17 13~~:42:~~01 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 22:03:51 2009''