2rjt - Revision history

OCA at 09:22, 21 February 2024

OCA — Wed, 21 Feb 2024 09:22:54 GMT

←Older revision		Revision as of 09:22, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='2rjt' size='340' side='right'caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2rjt' size='340' side='right'caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus~~ pneumoniae~~" (klein 1884) weichselbaum 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RJT FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>fabF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1313~~ "~~Diplococcus pneumoniae~~" ~~(Klein 1884) Weichselbaum 1886])</td~~>~~</tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-ketoacyl~~-[~~acyl-carrier-protein]_synthase_I Beta-ketoacyl-~~[~~acyl-carrier-protein~~] ~~synthase I~~]~~, with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.~~1.~~41 2.3.1.41] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [https://pdbe.org/2rjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [https://www.ebi.ac.uk/pdbsum/2rjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rjt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [https://pdbe.org/2rjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [https://www.ebi.ac.uk/pdbsum/2rjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q9FBC2_STREE Q9FBC2_STREE]] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP.[PIRNR:PIRNR000447]	+	[https://www.uniprot.org/uniprot/Q9FBC2_STREE Q9FBC2_STREE] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP.[PIRNR:PIRNR000447]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rjt ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rjt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A series of experiments with beta-ketoacyl acyl carrier protein synthase II (FabF) from Streptococcus pneumonia (spFabF) were undertaken to evaluate the capability of surface-entropy reduction (SER) to manipulate protein crystallization. Previous work has shown that this protein crystallizes in two forms. The triclinic form contains four molecules in the asymmetric unit (a.u.) and diffracts to 2.1 A resolution, while the more desirable primitive orthorhombic form contains one molecule in the a.u. and diffracts to 1.3 A. The aim was to evaluate the effect of SER mutations that were specifically engineered to avoid perturbing the crystal-packing interfaces employed by the favorable primitive orthorhombic crystal form while potentially disrupting a surface of the protein employed by the less desirable triclinic crystal form. Two mutant proteins were engineered, each of which harbored five SER mutations. Extensive crystallization screening produced crystals of the two mutants, but only under conditions that differed from those used for the native protein. One of the mutant proteins yielded crystals that were of a new form (centered orthorhombic), despite the fact that the interfaces employed by the primitive orthorhombic form of the native protein were specifically unaltered. Structure determination at 1.75 A resolution reveals that one of the mutations, E383A, appears to play a key role in disfavouring the less desirable triclinic crystal form and in generating a new surface for a packing interaction that stabilizes the new crystal form.
-
-	Surface-entropy reduction approaches to manipulate crystal forms of beta-ketoacyl acyl carrier protein synthase II from Streptococcus pneumoniae.,Parthasarathy G, Cummings R, Becker JW, Soisson SM Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):141-8. Epub 2008 Jan 16. PMID:18219113<ref>PMID:18219113</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2rjt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]		*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Becker, J~~]]	+	[[Category: Streptococcus pneumoniae]]
-	[[Category: ~~Parthasarathy, G~~]]	+	[[Category: Becker J]]
-	[[Category: ~~Soisson, S M~~]]	+	[[Category: Parthasarathy G]]
-	[[Category: ~~Acyltransferase]]~~	+	[[Category: Soisson SM]]
-	~~[[Category: Beta-ketoacyl acyl carrier protein synthase]]~~	+
-	~~[[Category: Fabf]]~~	+
-	~~[[Category: Kasii]]~~	+
-	~~[[Category: Thiolase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 04:28, 2 July 2021

OCA — Fri, 02 Jul 2021 04:28:06 GMT

←Older revision		Revision as of 04:28, 2 July 2021
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	==Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF==		==Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF==
-	<StructureSection load='2rjt' size='340' side='right' caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='2rjt' size='340' side='right'caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RJT FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [~~http~~://pdbe.org/2rjt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rjt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rjt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [https://pdbe.org/2rjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [https://www.ebi.ac.uk/pdbsum/2rjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Q9FBC2_STREE Q9FBC2_STREE]] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP.[PIRNR:PIRNR000447]	+	[[https://www.uniprot.org/uniprot/Q9FBC2_STREE Q9FBC2_STREE]] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP.[PIRNR:PIRNR000447]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rj/2rjt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rj/2rjt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 2rjt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2rjt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acyl carrier protein synthase 3D structures\|Acyl carrier protein synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Becker, J]]		[[Category: Becker, J]]
	[[Category: Parthasarathy, G]]		[[Category: Parthasarathy, G]]

OCA at 07:53, 25 October 2017

OCA — Wed, 25 Oct 2017 07:53:06 GMT

←Older revision		Revision as of 07:53, 25 October 2017
Line 1:		Line 1:
		+
	==Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF==		==Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF==
	<StructureSection load='2rjt' size='340' side='right' caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2rjt' size='340' side='right' caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [http://pdbe.org/2rjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [http://www.ebi.ac.uk/pdbsum/2rjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [http://pdbe.org/2rjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [http://www.ebi.ac.uk/pdbsum/2rjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 2rjt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2rjt" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 03:10, 9 February 2016

OCA — Tue, 09 Feb 2016 03:10:31 GMT

←Older revision		Revision as of 03:10, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2rjt' size='340' side='right' caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2rjt' size='340' side='right' caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_pneumoniae Streptococcus~~ pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RJT FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 ~~Streptococcus~~ pneumoniae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 "Diplococcus pneumoniae" (Klein 1884) Weichselbaum 1886])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-~~protein_synthase_I~~ Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [http://www.ebi.ac.uk/pdbsum/2rjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [http://pdbe.org/2rjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [http://www.ebi.ac.uk/pdbsum/2rjt PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q9FBC2_STRPN Q9FBC2_STRPN~~]] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP ~~(By similarity)~~.[PIRNR:PIRNR000447]	+	[[http://www.uniprot.org/uniprot/Q9FBC2_STREE Q9FBC2_STREE]] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP.[PIRNR:PIRNR000447]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rjt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2rjt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
-	[[Category: Streptococcus pneumoniae]]
	[[Category: Becker, J]]		[[Category: Becker, J]]
	[[Category: Parthasarathy, G]]		[[Category: Parthasarathy, G]]

OCA at 03:59, 25 December 2014

OCA — Thu, 25 Dec 2014 03:59:59 GMT

←Older revision		Revision as of 03:59, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RJT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RJT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [http://www.ebi.ac.uk/pdbsum/2rjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [http://www.ebi.ac.uk/pdbsum/2rjt PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q9FBC2_STRPN Q9FBC2_STRPN]] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP (By similarity).[PIRNR:PIRNR000447]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]		[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
-	[[Category: Becker, J.]]	+	[[Category: Becker, J]]
-	[[Category: Parthasarathy, G.]]	+	[[Category: Parthasarathy, G]]
-	[[Category: Soisson, S M.]]	+	[[Category: Soisson, S M]]
	[[Category: Acyltransferase]]		[[Category: Acyltransferase]]
	[[Category: Beta-ketoacyl acyl carrier protein synthase]]		[[Category: Beta-ketoacyl acyl carrier protein synthase]]

OCA at 09:08, 29 September 2014

OCA — Mon, 29 Sep 2014 09:08:52 GMT

←Older revision		Revision as of 09:08, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:2rjt.~~png\|left~~\|200px]]	+	==Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF==
		+	<StructureSection load='2rjt' size='340' side='right' caption='[[2rjt]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2rjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RJT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fabF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rjt RCSB], [http://www.ebi.ac.uk/pdbsum/2rjt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rj/2rjt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A series of experiments with beta-ketoacyl acyl carrier protein synthase II (FabF) from Streptococcus pneumonia (spFabF) were undertaken to evaluate the capability of surface-entropy reduction (SER) to manipulate protein crystallization. Previous work has shown that this protein crystallizes in two forms. The triclinic form contains four molecules in the asymmetric unit (a.u.) and diffracts to 2.1 A resolution, while the more desirable primitive orthorhombic form contains one molecule in the a.u. and diffracts to 1.3 A. The aim was to evaluate the effect of SER mutations that were specifically engineered to avoid perturbing the crystal-packing interfaces employed by the favorable primitive orthorhombic crystal form while potentially disrupting a surface of the protein employed by the less desirable triclinic crystal form. Two mutant proteins were engineered, each of which harbored five SER mutations. Extensive crystallization screening produced crystals of the two mutants, but only under conditions that differed from those used for the native protein. One of the mutant proteins yielded crystals that were of a new form (centered orthorhombic), despite the fact that the interfaces employed by the primitive orthorhombic form of the native protein were specifically unaltered. Structure determination at 1.75 A resolution reveals that one of the mutations, E383A, appears to play a key role in disfavouring the less desirable triclinic crystal form and in generating a new surface for a packing interaction that stabilizes the new crystal form.

-	~~{{STRUCTURE_2rjt\| PDB=2rjt \| SCENE= }}~~	+	Surface-entropy reduction approaches to manipulate crystal forms of beta-ketoacyl acyl carrier protein synthase II from Streptococcus pneumoniae.,Parthasarathy G, Cummings R, Becker JW, Soisson SM Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):141-8. Epub 2008 Jan 16. PMID:18219113<ref>PMID:18219113</ref>

-	~~===Crystal Structure Analysis of~~ a ~~Surface Entropy Reduction Mutant~~ of S. ~~pneumoniae FabF===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18219113}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2rjt]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA].~~	+

	==See Also==		==See Also==
	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]		*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018219113<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]		[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]

OCA at 12:26, 20 October 2012

OCA — Sat, 20 Oct 2012 12:26:34 GMT

←Older revision		Revision as of 12:26, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2rjt]] is a 4 chain structure ~~of [[Acyl carrier protein synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA].	+	[[2rjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA].

	==See Also==		==See Also==

OCA at 08:43, 26 July 2012

OCA — Thu, 26 Jul 2012 08:43:18 GMT

←Older revision		Revision as of 08:43, 26 July 2012
Line 1:		Line 1:
	[[Image:2rjt.png\|left\|200px]]		[[Image:2rjt.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2rjt", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2rjt\| PDB=2rjt \| SCENE= }}		{{STRUCTURE_2rjt\| PDB=2rjt \| SCENE= }}

	===Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF===		===Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18219113}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18219113 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18219113}}		{{ABSTRACT_PUBMED_18219113}}

	==About this Structure==		==About this Structure==
-	[[2rjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA].	+	[[2rjt]] is a 4 chain structure of [[Acyl carrier protein synthase]] with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA].
		+
		+	==See Also==
		+	*[[Acyl carrier protein synthase\|Acyl carrier protein synthase]]

	==Reference==		==Reference==

OCA: Protected "2rjt" [edit=sysop:move=sysop]

OCA — Wed, 28 Mar 2012 06:36:56 GMT

Protected "2rjt" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:36, 28 March 2012

OCA at 06:36, 28 March 2012

OCA — Wed, 28 Mar 2012 06:36:54 GMT

←Older revision		Revision as of 06:36, 28 March 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2rjt.png\|left\|200px]]		[[Image:2rjt.png\|left\|200px]]

Line 12:		Line 11:
	===Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF===		===Crystal Structure Analysis of a Surface Entropy Reduction Mutant of S. pneumoniae FabF===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18219113}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18219113 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18219113}}

	==About this Structure==		==About this Structure==
-	~~2RJT~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA].	+	[[2rjt]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RJT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:018219113</ref><references group="xtra"/>
	[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]		[[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
Line 26:		Line 34:
	[[Category: Thiolase]]		[[Category: Thiolase]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:59:27 2009''