2rnm - Revision history

OCA at 07:05, 1 May 2024

OCA — Wed, 01 May 2024 07:05:27 GMT

←Older revision		Revision as of 07:05, 1 May 2024
Line 1:		Line 1:

	==Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR==		==Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR==
-	<StructureSection load='2rnm' size='340' side='right'caption='[[2rnm~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2rnm' size='340' side='right'caption='[[2rnm]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Podas Podas~~]. The May 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RNM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Podospora_anserina Podospora anserina]. The May 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RNM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [https://pdbe.org/2rnm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [https://www.ebi.ac.uk/pdbsum/2rnm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rnm ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [https://pdbe.org/2rnm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [https://www.ebi.ac.uk/pdbsum/2rnm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rnm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>	+	[https://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Prion and nonprion forms of proteins are believed to differ solely in their three-dimensional structure, which is therefore of paramount importance for the prion function. However, no atomic-resolution structure of the fibrillar state that is likely infectious has been reported to date. We present a structural model based on solid-state nuclear magnetic resonance restraints for amyloid fibrils from the prion-forming domain (residues 218 to 289) of the HET-s protein from the filamentous fungus Podospora anserina. On the basis of 134 intra- and intermolecular experimental distance restraints, we find that HET-s(218-289) forms a left-handed beta solenoid, with each molecule forming two helical windings, a compact hydrophobic core, at least 23 hydrogen bonds, three salt bridges, and two asparagine ladders. The structure is likely to have broad implications for understanding the infectious amyloid state.	+
-		+
-	Amyloid fibrils of the HET-s(218-289) prion form a beta solenoid with a triangular hydrophobic core.,Wasmer C, Lange A, Van Melckebeke H, Siemer AB, Riek R, Meier BH Science. 2008 Mar 14;319(5869):1523-6. PMID:18339938<ref>PMID:18339938</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 2rnm" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 26:		Line 18:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Podas~~]]	+	[[Category: Podospora anserina]]
	[[Category: Prions]]		[[Category: Prions]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Lange, A]]	+	[[Category: Lange A]]
-	[[Category: Meier~~, B H]]~~	+	[[Category: Meier BH]]
-	~~[[Category: Melckebeke, H Van~~]]	+	[[Category: Riek R]]
-	[[Category: Riek, R]]	+	[[Category: Siemer A]]
-	[[Category: Siemer, A]]	+	[[Category: Van Melckebeke H]]
-	[[Category: ~~Wasmer, C~~]]	+	[[Category: Wasmer C]]
-	[[Category: ~~Amyloid fibril]]~~	+
-	~~[[Category: Asparagine ladder]]~~	+
-	~~[[Category: Beta-helix]]~~	+
-	~~[[Category: Beta-solenoid]]~~	+
-	~~[[Category: Hydrophobic core]]~~	+
-	~~[[Category: Parallel beta-sheet]]~~	+
-	~~[[Category: Prion]]~~	+
-	~~[[Category: Protein fibril]]~~	+
-	~~[[Category: Salt bridge~~]]	+

OCA at 18:31, 27 July 2022

OCA — Wed, 27 Jul 2022 18:31:03 GMT

←Older revision		Revision as of 18:31, 27 July 2022
Line 3:		Line 3:
	<StructureSection load='2rnm' size='340' side='right'caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rnm' size='340' side='right'caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Podas Podas]. The May 2008 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2RNM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Podas Podas]. The May 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RNM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [~~http~~://pdbe.org/2rnm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rnm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rnm ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [https://pdbe.org/2rnm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [https://www.ebi.ac.uk/pdbsum/2rnm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rnm ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>	+	[[https://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:56, 21 October 2020

OCA — Wed, 21 Oct 2020 08:56:02 GMT

←Older revision		Revision as of 08:56, 21 October 2020
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR==		==Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR==
-	<StructureSection load='2rnm' size='340' side='right' caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2rnm' size='340' side='right'caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Podas Podas]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RNM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Podas Podas]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2RNM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://pdbe.org/2rnm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rnm ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://pdbe.org/2rnm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rnm ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>		[[http://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>
Line 20:		Line 19:

	==See Also==		==See Also==
-	*[[Prion\|Prion]]	+	*[[Prion 3D structures\|Prion 3D structures]]
	*[[Prion protein\|Prion protein]]		*[[Prion protein\|Prion protein]]
	== References ==		== References ==
Line 26:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Podas]]		[[Category: Podas]]
	[[Category: Prions]]		[[Category: Prions]]

OCA at 12:10, 12 April 2017

OCA — Wed, 12 Apr 2017 12:10:21 GMT

←Older revision		Revision as of 12:10, 12 April 2017
Line 1:		Line 1:
		+	{{Large structure}}
	==Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR==		==Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR==
	<StructureSection load='2rnm' size='340' side='right' caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rnm' size='340' side='right' caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Podas Podas]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RNM FirstGlance]. <br>		<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Podas Podas]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RNM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://pdbe.org/2rnm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rnm ProSAT]</span></td></tr>
	</table>		</table>
		+	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>		[[http://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>
Line 15:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2rnm" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 08:44, 17 June 2015

OCA — Wed, 17 Jun 2015 08:44:04 GMT

←Older revision		Revision as of 08:44, 17 June 2015
Line 2:		Line 2:
	<StructureSection load='2rnm' size='340' side='right' caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rnm' size='340' side='right' caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Podospora_anserina Podospora anserina~~]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RNM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Podas Podas]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RNM FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q03689_PODAN Q03689_PODAN~~]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>	+	[[http://www.uniprot.org/uniprot/HETS_PODAS HETS_PODAS]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Podospora anserina~~]]	+	[[Category: Podas]]
	[[Category: Prions]]		[[Category: Prions]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]

OCA at 10:33, 25 December 2014

OCA — Thu, 25 Dec 2014 10:33:20 GMT

←Older revision		Revision as of 10:33, 25 December 2014
Line 5:		Line 5:
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q03689_PODAN Q03689_PODAN]] Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.<ref>PMID:1886611</ref> <ref>PMID:8224826</ref> <ref>PMID:9275200</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 14:12, 17 December 2014

OCA — Wed, 17 Dec 2014 14:12:07 GMT

←Older revision		Revision as of 14:12, 17 December 2014
Line 1:		Line 1:
-	~~{{Large structure}}~~	+	==Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR==
-	~~{{STRUCTURE_2rnm\| PDB=2rnm \| SCENE= }}~~	+	<StructureSection load='2rnm' size='340' side='right' caption='[[2rnm]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	===Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR===	+	== Structural highlights ==
-	~~{{ABSTRACT_PUBMED_18339938}}~~	+	<table><tr><td colspan='2'>[[2rnm]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Podospora_anserina Podospora anserina]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RNM FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rnm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rnm RCSB], [http://www.ebi.ac.uk/pdbsum/2rnm PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Prion and nonprion forms of proteins are believed to differ solely in their three-dimensional structure, which is therefore of paramount importance for the prion function. However, no atomic-resolution structure of the fibrillar state that is likely infectious has been reported to date. We present a structural model based on solid-state nuclear magnetic resonance restraints for amyloid fibrils from the prion-forming domain (residues 218 to 289) of the HET-s protein from the filamentous fungus Podospora anserina. On the basis of 134 intra- and intermolecular experimental distance restraints, we find that HET-s(218-289) forms a left-handed beta solenoid, with each molecule forming two helical windings, a compact hydrophobic core, at least 23 hydrogen bonds, three salt bridges, and two asparagine ladders. The structure is likely to have broad implications for understanding the infectious amyloid state.

-	~~==About this Structure==~~	+	Amyloid fibrils of the HET-s(218-289) prion form a beta solenoid with a triangular hydrophobic core.,Wasmer C, Lange A, Van Melckebeke H, Siemer AB, Riek R, Meier BH Science. 2008 Mar 14;319(5869):1523-6. PMID:18339938<ref>PMID:18339938</ref>
-	~~[[2rnm]] is~~ a ~~5 chain structure~~ with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Podospora_anserina Podospora anserina]. The May~~ 2008 ~~RCSB PDB [http~~:~~//pdb~~.~~rcsb.org~~/~~pdb~~/~~static.do?p=education_discussion/molecule_of_the_month/index.html Molecule~~ of the ~~Month] feature on ''Prions'' by David~~ S. ~~Goodsell is [http://dx~~.~~doi.org~~/~~10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
-	*[[Human Prion Protein Dimer\|Human Prion Protein Dimer]]
	*[[Prion\|Prion]]		*[[Prion\|Prion]]
	*[[Prion protein\|Prion protein]]		*[[Prion protein\|Prion protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018339938<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Podospora anserina]]		[[Category: Podospora anserina]]
	[[Category: Prions]]		[[Category: Prions]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Lange, A.]]	+	[[Category: Lange, A]]
-	[[Category: Meier, B H.]]	+	[[Category: Meier, B H]]
-	[[Category: Melckebeke, H Van.]]	+	[[Category: Melckebeke, H Van]]
-	[[Category: Riek, R.]]	+	[[Category: Riek, R]]
-	[[Category: Siemer, A.]]	+	[[Category: Siemer, A]]
-	[[Category: Wasmer, C.]]	+	[[Category: Wasmer, C]]
	[[Category: Amyloid fibril]]		[[Category: Amyloid fibril]]
	[[Category: Asparagine ladder]]		[[Category: Asparagine ladder]]

OCA at 08:40, 11 March 2013

OCA — Mon, 11 Mar 2013 08:40:03 GMT

←Older revision		Revision as of 08:40, 11 March 2013
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-	~~[[Image:2rnm.png\|left\|200px]]~~	+	{{Large structure}}
-		+
	{{STRUCTURE_2rnm\| PDB=2rnm \| SCENE= }}		{{STRUCTURE_2rnm\| PDB=2rnm \| SCENE= }}
-
	===Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR===		===Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR===
-
	{{ABSTRACT_PUBMED_18339938}}		{{ABSTRACT_PUBMED_18339938}}

Line 11:		Line 8:

	==See Also==		==See Also==
		+	*[[Human Prion Protein Dimer\|Human Prion Protein Dimer]]
	*[[Prion\|Prion]]		*[[Prion\|Prion]]
	*[[Prion protein\|Prion protein]]		*[[Prion protein\|Prion protein]]

OCA at 15:41, 20 October 2012

OCA — Sat, 20 Oct 2012 15:41:05 GMT

←Older revision		Revision as of 15:41, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2rnm]] is a 5 chain structure ~~of [[Prion]] and [[Prion protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Podospora_anserina Podospora anserina]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA].	+	[[2rnm]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Podospora_anserina Podospora anserina]. The May 2008 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Prions'' by David S. Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2008_5 10.2210/rcsb_pdb/mom_2008_5]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RNM OCA].

	==See Also==		==See Also==

OCA at 19:09, 26 July 2012

OCA — Thu, 26 Jul 2012 19:09:42 GMT

←Older revision		Revision as of 19:09, 26 July 2012
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	[[Image:2rnm.png\|left\|200px]]		[[Image:2rnm.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2rnm\| PDB=2rnm \| SCENE= }}		{{STRUCTURE_2rnm\| PDB=2rnm \| SCENE= }}

	===Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR===		===Structure of The HET-s(218-289) prion in its amyloid form obtained by solid-state NMR===

-
-	<!--
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	{{ABSTRACT_PUBMED_18339938}}		{{ABSTRACT_PUBMED_18339938}}

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	==See Also==		==See Also==
-	*[[Prion]]	+	*[[Prion\|Prion]]
-	*[[Prion protein]]	+	*[[Prion protein\|Prion protein]]

	==Reference==		==Reference==