2rqw - Revision history

OCA at 07:06, 1 May 2024

OCA — Wed, 01 May 2024 07:06:05 GMT

←Older revision		Revision as of 07:06, 1 May 2024
Line 1:		Line 1:

	==Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide==		==Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide==
-	<StructureSection load='2rqw' size='340' side='right'caption='[[2rqw~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='2rqw' size='340' side='right'caption='[[2rqw]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQW FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[2rqv\|2rqv]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [https://pdbe.org/2rqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [https://www.ebi.ac.uk/pdbsum/2rqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [https://pdbe.org/2rqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [https://www.ebi.ac.uk/pdbsum/2rqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BEM1_YEAST BEM1_YEAST]] Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.<ref>PMID:17460121</ref> [[https://www.uniprot.org/uniprot/STE20_YEAST STE20_YEAST]] MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H(2)O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling.<ref>PMID:1464311</ref> <ref>PMID:8421676</ref> <ref>PMID:7608157</ref> <ref>PMID:9003780</ref> <ref>PMID:8722766</ref> <ref>PMID:8643578</ref> <ref>PMID:9009270</ref> <ref>PMID:9388196</ref> <ref>PMID:9742399</ref> <ref>PMID:9428767</ref> <ref>PMID:10562285</ref> <ref>PMID:10837245</ref> <ref>PMID:10970855</ref> <ref>PMID:11003652</ref> <ref>PMID:11940652</ref> <ref>PMID:12686605</ref> <ref>PMID:12588977</ref> <ref>PMID:15652479</ref> <ref>PMID:15970663</ref> <ref>PMID:15743816</ref>	+	[https://www.uniprot.org/uniprot/BEM1_YEAST BEM1_YEAST] Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.<ref>PMID:17460121</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rqw ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rqw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bem1 is a scaffold protein essential for the establishment of cell polarity in Saccharomyces cerevisiae. This work reports the solution structure of a Cdc42 binding module of Bem1 comprising the second SH3 domain (SH3b) and its C-terminal flanking region termed Cdc42 interacting (CI). First, the structure of Bem1 SH3b-CI was determined by NMR spectroscopy, which shows that Bem1 SH3b-CI is a structurally and functionally related domain that binds Cdc42. Next, the solution structure of Bem1 SH3b-CI in complex with the proline-rich region of p21-activated kinase Ste20 (Ste20 PRR) was determined. Finally, the interaction surface of Bem1 SH3b-CI with Cdc42 was identified based on chemical shift perturbation studies which reveals that Bem1 SH3b-CI interacts simultaneously with both Ste20 PRR and Cdc42 using the opposite surfaces. Thus, Bem1 can tether Cdc42 and Ste20 in close proximity so that Cdc42 can efficiently interact with Ste20 Cdc42 and Rac interactive binding (CRIB). Based on the present results together with the previous biochemical studies (Lamson, R. E., Winters, M. J., and Pryciak, P. M. (2002) Mol. Cell. Biol. 22, 2939-2951 and Winters, M. J., and Pryciak, P. M. (2005) Mol. Cell. Biol. 25, 2177-2190), a model was suggested that the autoinhibition of Ste20 kinase activity by CRIB is released through the Cdc42-CRIB interaction, which is mediated by Bem1, and Ste20 is subsequently activated, an initial step for the establishment of the cell polarity.
-
-	Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42.,Takaku T, Ogura K, Kumeta H, Yoshida N, Inagaki F J Biol Chem. 2010 Jun 18;285(25):19346-53. Epub 2010 Apr 21. PMID:20410294<ref>PMID:20410294</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2rqw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Inagaki, F~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Ogura, K~~]]	+	[[Category: Inagaki F]]
-	[[Category: ~~Takaku, T~~]]	+	[[Category: Ogura K]]
-	[[Category: ~~Bem1p]]~~	+	[[Category: Takaku T]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Cytoskeleton]]~~	+
-	~~[[Category: Pheromone response]]~~	+
-	~~[[Category: Prr]]~~	+
-	~~[[Category: Serine/threonine-protein kinase]]~~	+
-	~~[[Category: Sh3 domain]]~~	+
-	~~[[Category: Sh3ci]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Ste20p~~]]	+

OCA at 12:25, 23 March 2022

OCA — Wed, 23 Mar 2022 12:25:29 GMT

←Older revision		Revision as of 12:25, 23 March 2022
Line 3:		Line 3:
	<StructureSection load='2rqw' size='340' side='right'caption='[[2rqw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rqw' size='340' side='right'caption='[[2rqw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2RQW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RQW FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rqv\|2rqv]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2rqv\|2rqv]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [~~http~~://pdbe.org/2rqw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2rqw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [https://pdbe.org/2rqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [https://www.ebi.ac.uk/pdbsum/2rqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BEM1_YEAST BEM1_YEAST]] Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.<ref>PMID:17460121</ref> [[~~http~~://www.uniprot.org/uniprot/STE20_YEAST STE20_YEAST]] MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H(2)O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling.<ref>PMID:1464311</ref> <ref>PMID:8421676</ref> <ref>PMID:7608157</ref> <ref>PMID:9003780</ref> <ref>PMID:8722766</ref> <ref>PMID:8643578</ref> <ref>PMID:9009270</ref> <ref>PMID:9388196</ref> <ref>PMID:9742399</ref> <ref>PMID:9428767</ref> <ref>PMID:10562285</ref> <ref>PMID:10837245</ref> <ref>PMID:10970855</ref> <ref>PMID:11003652</ref> <ref>PMID:11940652</ref> <ref>PMID:12686605</ref> <ref>PMID:12588977</ref> <ref>PMID:15652479</ref> <ref>PMID:15970663</ref> <ref>PMID:15743816</ref>	+	[[https://www.uniprot.org/uniprot/BEM1_YEAST BEM1_YEAST]] Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.<ref>PMID:17460121</ref> [[https://www.uniprot.org/uniprot/STE20_YEAST STE20_YEAST]] MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H(2)O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling.<ref>PMID:1464311</ref> <ref>PMID:8421676</ref> <ref>PMID:7608157</ref> <ref>PMID:9003780</ref> <ref>PMID:8722766</ref> <ref>PMID:8643578</ref> <ref>PMID:9009270</ref> <ref>PMID:9388196</ref> <ref>PMID:9742399</ref> <ref>PMID:9428767</ref> <ref>PMID:10562285</ref> <ref>PMID:10837245</ref> <ref>PMID:10970855</ref> <ref>PMID:11003652</ref> <ref>PMID:11940652</ref> <ref>PMID:12686605</ref> <ref>PMID:12588977</ref> <ref>PMID:15652479</ref> <ref>PMID:15970663</ref> <ref>PMID:15743816</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:04, 17 June 2020

OCA — Wed, 17 Jun 2020 11:04:26 GMT

←Older revision		Revision as of 11:04, 17 June 2020
Line 1:		Line 1:

	==Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide==		==Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide==
-	<StructureSection load='2rqw' size='340' side='right' caption='[[2rqw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='2rqw' size='340' side='right'caption='[[2rqw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2RQW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2RQW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rqv\|2rqv]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rqv\|2rqv]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [http://pdbe.org/2rqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [http://www.ebi.ac.uk/pdbsum/2rqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [http://pdbe.org/2rqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [http://www.ebi.ac.uk/pdbsum/2rqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Inagaki, F]]		[[Category: Inagaki, F]]
	[[Category: Ogura, K]]		[[Category: Ogura, K]]

OCA at 08:10, 12 September 2018

OCA — Wed, 12 Sep 2018 08:10:57 GMT

←Older revision		Revision as of 08:10, 12 September 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rq/2rqw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rq/2rqw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 38:		Line 38:
	[[Category: Bem1p]]		[[Category: Bem1p]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
		+	[[Category: Cytoplasm]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Pheromone response]]		[[Category: Pheromone response]]

OCA at 16:56, 5 August 2016

OCA — Fri, 05 Aug 2016 16:56:01 GMT

←Older revision		Revision as of 16:56, 5 August 2016
Line 1:		Line 1:
		+
	==Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide==		==Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide==
	<StructureSection load='2rqw' size='340' side='right' caption='[[2rqw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='2rqw' size='340' side='right' caption='[[2rqw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RQW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RQW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rqv\|2rqv]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rqv\|2rqv]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [http://www.ebi.ac.uk/pdbsum/2rqw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [http://pdbe.org/2rqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [http://www.ebi.ac.uk/pdbsum/2rqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2rqw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rqw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2rqw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Inagaki, F]]		[[Category: Inagaki, F]]
	[[Category: Ogura, K]]		[[Category: Ogura, K]]

OCA at 20:28, 25 December 2014

OCA — Thu, 25 Dec 2014 20:28:18 GMT

←Older revision		Revision as of 20:28, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [http://www.ebi.ac.uk/pdbsum/2rqw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [http://www.ebi.ac.uk/pdbsum/2rqw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BEM1_YEAST BEM1_YEAST]] Necessary for cell polarization during vegetative growth. May link the cytoskeleton to morphogenic determinants on the cell surface.<ref>PMID:17460121</ref> [[http://www.uniprot.org/uniprot/STE20_YEAST STE20_YEAST]] MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H(2)O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling.<ref>PMID:1464311</ref> <ref>PMID:8421676</ref> <ref>PMID:7608157</ref> <ref>PMID:9003780</ref> <ref>PMID:8722766</ref> <ref>PMID:8643578</ref> <ref>PMID:9009270</ref> <ref>PMID:9388196</ref> <ref>PMID:9742399</ref> <ref>PMID:9428767</ref> <ref>PMID:10562285</ref> <ref>PMID:10837245</ref> <ref>PMID:10970855</ref> <ref>PMID:11003652</ref> <ref>PMID:11940652</ref> <ref>PMID:12686605</ref> <ref>PMID:12588977</ref> <ref>PMID:15652479</ref> <ref>PMID:15970663</ref> <ref>PMID:15743816</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Inagaki, F.]]	+	[[Category: Inagaki, F]]
-	[[Category: Ogura, K.]]	+	[[Category: Ogura, K]]
-	[[Category: Takaku, T.]]	+	[[Category: Takaku, T]]
	[[Category: Bem1p]]		[[Category: Bem1p]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]

OCA at 11:18, 20 October 2014

OCA — Mon, 20 Oct 2014 11:18:00 GMT

←Older revision		Revision as of 11:18, 20 October 2014
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-	[[~~Image:~~2rqw.~~png~~\|~~left~~\|200px]]	+	==Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide==
		+	<StructureSection load='2rqw' size='340' side='right' caption='[[2rqw]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2rqw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RQW FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2rqv\|2rqv]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rqw RCSB], [http://www.ebi.ac.uk/pdbsum/2rqw PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rq/2rqw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bem1 is a scaffold protein essential for the establishment of cell polarity in Saccharomyces cerevisiae. This work reports the solution structure of a Cdc42 binding module of Bem1 comprising the second SH3 domain (SH3b) and its C-terminal flanking region termed Cdc42 interacting (CI). First, the structure of Bem1 SH3b-CI was determined by NMR spectroscopy, which shows that Bem1 SH3b-CI is a structurally and functionally related domain that binds Cdc42. Next, the solution structure of Bem1 SH3b-CI in complex with the proline-rich region of p21-activated kinase Ste20 (Ste20 PRR) was determined. Finally, the interaction surface of Bem1 SH3b-CI with Cdc42 was identified based on chemical shift perturbation studies which reveals that Bem1 SH3b-CI interacts simultaneously with both Ste20 PRR and Cdc42 using the opposite surfaces. Thus, Bem1 can tether Cdc42 and Ste20 in close proximity so that Cdc42 can efficiently interact with Ste20 Cdc42 and Rac interactive binding (CRIB). Based on the present results together with the previous biochemical studies (Lamson, R. E., Winters, M. J., and Pryciak, P. M. (2002) Mol. Cell. Biol. 22, 2939-2951 and Winters, M. J., and Pryciak, P. M. (2005) Mol. Cell. Biol. 25, 2177-2190), a model was suggested that the autoinhibition of Ste20 kinase activity by CRIB is released through the Cdc42-CRIB interaction, which is mediated by Bem1, and Ste20 is subsequently activated, an initial step for the establishment of the cell polarity.

-	~~{{STRUCTURE_2rqw\| PDB=2rqw \| SCENE= }}~~	+	Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42.,Takaku T, Ogura K, Kumeta H, Yoshida N, Inagaki F J Biol Chem. 2010 Jun 18;285(25):19346-53. Epub 2010 Apr 21. PMID:20410294<ref>PMID:20410294</ref>

-	~~===Solution structure~~ of ~~Bem1p SH3CI domain complexed with Ste20p-PRR peptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20410294}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2rqw]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020410294<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Inagaki, F.]]		[[Category: Inagaki, F.]]

OCA at 19:53, 7 January 2013

OCA — Mon, 07 Jan 2013 19:53:33 GMT

←Older revision		Revision as of 19:53, 7 January 2013
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	[[Image:2rqw.png\|left\|200px]]		[[Image:2rqw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2rqw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2rqw\| PDB=2rqw \| SCENE= }}		{{STRUCTURE_2rqw\| PDB=2rqw \| SCENE= }}

	===Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide===		===Solution structure of Bem1p SH3CI domain complexed with Ste20p-PRR peptide===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20410294}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20410294 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20410294}}		{{ABSTRACT_PUBMED_20410294}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20410294~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020410294</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Inagaki, F.]]		[[Category: Inagaki, F.]]
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	[[Category: Bem1p]]		[[Category: Bem1p]]
	[[Category: Cell cycle]]		[[Category: Cell cycle]]
-	[[Category: Cytoplasm]]
	[[Category: Cytoskeleton]]		[[Category: Cytoskeleton]]
	[[Category: Pheromone response]]		[[Category: Pheromone response]]

OCA: Protected "2rqw" [edit=sysop:move=sysop]

OCA — Sun, 02 Jan 2011 03:15:40 GMT

Protected "2rqw" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:15, 2 January 2011

OCA at 03:15, 2 January 2011

OCA — Sun, 02 Jan 2011 03:15:38 GMT

←Older revision		Revision as of 03:15, 2 January 2011
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-	{{Seed}}
	[[Image:2rqw.png\|left\|200px]]		[[Image:2rqw.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~2RQW~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA].	+	[[2rqw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RQW OCA].

	==Reference==		==Reference==
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	[[Category: Signaling protein]]		[[Category: Signaling protein]]
	[[Category: Ste20p]]		[[Category: Ste20p]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 5 11:14:00 2010''