2v5q - Revision history

OCA at 15:06, 13 December 2023

OCA — Wed, 13 Dec 2023 15:06:32 GMT

←Older revision		Revision as of 15:06, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2v5q' size='340' side='right'caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2v5q' size='340' side='right'caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~] and [https://en.wikipedia.org/wiki/~~Miscellaneous_nucleic_acid Miscellaneous nucleic acid~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1q4k\|1q4k]]~~, [[~~1q4o~~\|~~1q4o~~]]~~, [[1umw\|1umw]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.~~7.11.21 2.7.11.21] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [https://pdbe.org/2v5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [https://www.ebi.ac.uk/pdbsum/2v5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [https://pdbe.org/2v5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [https://www.ebi.ac.uk/pdbsum/2v5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/PLK1_HUMAN PLK1_HUMAN] Note=Defects in PLK1 are associated with some cancers, such as gastric, thyroid or B-cell lymphomas. Expression is cancer increased in tumor tissues with a poor prognosis, suggesting a role in malignant transformations and carcinogenesis.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PLK1_HUMAN PLK1_HUMAN] Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, MLF1IP, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, PLK1S1/KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating PLK1S1/KIZ, NEDD1 and NINL. NEDD1 phosphorylation promotes subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. Phosphorylation of NINL component of the centrosome leads to NINL dissociation from other centrosomal proteins. Involved in mitosis exit and cytokinesis by phosphorylating CEP55, ECT2, KIF20A/MKLP2, MLF1IP, PRC1 and RACGAP1. Recruited at the central spindle by phosphorylating and docking PRC1 and KIF20A/MKLP2; creates its own docking sites on PRC1 and KIF20A/MKLP2 by mediating phosphorylation of sites subsequently recognized by the POLO box domains. Phosphorylates RACGAP1, thereby creating a docking site for the Rho GTP exchange factor ECT2 that is essential for the cleavage furrow formation. Promotes the central spindle recruitment of ECT2. Plays a central role in G2/M transition of mitotic cell cycle by phosphorylating CCNB1, CDC25C, FOXM1, MLF1IP, PKMYT1/MYT1, PPP1R12A/MYPT1 and WEE1. Part of a regulatory circuit that promotes the activation of CDK1 by phosphorylating the positive regulator CDC25C and inhibiting the negative regulators WEE1 and PKMYT1/MYT1. Also acts by mediating phosphorylation of cyclin-B1 (CCNB1) on centrosomes in prophase. Phosphorylates FOXM1, a key mitotic transcription regulator, leading to enhance FOXM1 transcriptional activity. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating BUB1B/BUBR1, FBXO5/EMI1 and STAG2/SA2. PLK1 is high on non-attached kinetochores suggesting a role of PLK1 in kinetochore attachment or in spindle assembly checkpoint (SAC) regulation. Required for kinetochore localization of BUB1B. Regulates the dissociation of cohesin from chromosomes by phosphorylating cohesin subunits such as STAG2/SA2. Phosphorylates SGOL1: required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Mediates phosphorylation of FBXO5/EMI1, a negative regulator of the APC/C complex during prophase, leading to FBXO5/EMI1 ubiquitination and degradation by the proteasome. Acts as a negative regulator of p53 family members: phosphorylates TOPORS, leading to inhibit the sumoylation of p53/TP53 and simultaneously enhance the ubiquitination and subsequent degradation of p53/TP53. Phosphorylates the transactivation domain of the transcription factor p73/TP73, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Also required for recovery after DNA damage checkpoint and entry into mitosis.<ref>PMID:8991084</ref> <ref>PMID:11202906</ref> <ref>PMID:12207013</ref> <ref>PMID:12447691</ref> <ref>PMID:12852856</ref> <ref>PMID:12738781</ref> <ref>PMID:12939256</ref> <ref>PMID:12524548</ref> <ref>PMID:14734534</ref> <ref>PMID:15469984</ref> <ref>PMID:15070733</ref> <ref>PMID:15148369</ref> <ref>PMID:16198290</ref> <ref>PMID:16980960</ref> <ref>PMID:16247472</ref> <ref>PMID:17081991</ref> <ref>PMID:17617734</ref> <ref>PMID:17376779</ref> <ref>PMID:17351640</ref> <ref>PMID:18418051</ref> <ref>PMID:18521620</ref> <ref>PMID:18331714</ref> <ref>PMID:18477460</ref> <ref>PMID:18174154</ref> <ref>PMID:19160488</ref> <ref>PMID:18615013</ref> <ref>PMID:19473992</ref> <ref>PMID:19509060</ref> <ref>PMID:19351716</ref> <ref>PMID:19468300</ref> <ref>PMID:19468302</ref> <ref>PMID:14532005</ref> <ref>PMID:19597481</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Miscellaneous nucleic acid]]~~	+	[[Category: Synthetic construct]]
-	~~[[Category: Polo kinase~~]]	+	[[Category: Amstutz P]]
-	[[Category: Amstutz, P]]	+	[[Category: Bandeiras TM]]
-	[[Category: Bandeiras~~, T M~~]]	+	[[Category: Binz HK]]
-	[[Category: Binz~~, H K~~]]	+	[[Category: Carrondo MA]]
-	[[Category: Carrondo~~, M A~~]]	+	[[Category: Crusius K]]
-	[[Category: Crusius, K]]	+	[[Category: Donner P]]
-	[[Category: Donner, P]]	+	[[Category: Eberspaecher U]]
-	[[Category: Eberspaecher, U]]	+	[[Category: Egner U]]
-	[[Category: Egner, U]]	+	[[Category: Fanghaenel J]]
-	[[Category: Fanghaenel, J]]	+	[[Category: Gulotti-Georgieva M]]
-	[[Category: Gulotti-Georgieva, M]]	+	[[Category: Hillig RC]]
-	[[Category: Hillig~~, R C~~]]	+	[[Category: Holz C]]
-	[[Category: Holz, C]]	+	[[Category: Lang C]]
-	[[Category: Lang, C]]	+	[[Category: Matias PM]]
-	[[Category: Matias~~, P M~~]]	+	[[Category: Miranda S]]
-	[[Category: Miranda, S]]	+	[[Category: Mueller-Tiemann B]]
-	[[Category: Mueller-Tiemann, B]]	+	[[Category: Puetter V]]
-	[[Category: Puetter, V]]	+	[[Category: Schmitz AAP]]
-	[[Category: Schmitz~~, A A.P~~]]	+	[[Category: Thomaz M]]
-	[[Category: Thomaz, M]]	+
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Design ankyrin repeat protein]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphorylation]]~~	+
-	~~[[Category: Serine/threonine protein kinase]]~~	+
-	~~[[Category: Serine/threonine-protein kinase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transferase complex~~]]	+

OCA at 10:42, 7 July 2021

OCA — Wed, 07 Jul 2021 10:42:05 GMT

←Older revision		Revision as of 10:42, 7 July 2021
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	==CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN==		==CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN==
-	<StructureSection load='2v5q' size='340' side='right' caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='2v5q' size='340' side='right'caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human] and [~~http~~://en.wikipedia.org/wiki/Miscellaneous_nucleic_acid Miscellaneous nucleic acid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human] and [https://en.wikipedia.org/wiki/Miscellaneous_nucleic_acid Miscellaneous nucleic acid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [~~http~~://pdbe.org/2v5q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2v5q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [https://pdbe.org/2v5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [https://www.ebi.ac.uk/pdbsum/2v5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5q ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]	+	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Miscellaneous nucleic acid]]		[[Category: Miscellaneous nucleic acid]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]

OCA at 09:02, 12 September 2018

OCA — Wed, 12 Sep 2018 09:02:01 GMT

←Older revision		Revision as of 09:02, 12 September 2018
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN==		==CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN==
	<StructureSection load='2v5q' size='340' side='right' caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2v5q' size='340' side='right' caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>
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	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [http://pdbe.org/2v5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [http://www.ebi.ac.uk/pdbsum/2v5q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [http://pdbe.org/2v5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [http://www.ebi.ac.uk/pdbsum/2v5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v5q ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v5/2v5q_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v5/2v5q_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 21:02, 8 February 2016

OCA — Mon, 08 Feb 2016 21:02:59 GMT

←Older revision		Revision as of 21:02, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='2v5q' size='340' side='right' caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2v5q' size='340' side='right' caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~] and [http://en.wikipedia.org/wiki/~~Unidentified Unidentified~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Miscellaneous_nucleic_acid Miscellaneous nucleic acid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [http://www.ebi.ac.uk/pdbsum/2v5q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [http://pdbe.org/2v5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [http://www.ebi.ac.uk/pdbsum/2v5q PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v5q ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2v5q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
		+	[[Category: Miscellaneous nucleic acid]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]
-	[[Category: Unidentified]]
	[[Category: Amstutz, P]]		[[Category: Amstutz, P]]
	[[Category: Bandeiras, T M]]		[[Category: Bandeiras, T M]]

OCA at 17:13, 19 January 2015

OCA — Mon, 19 Jan 2015 17:13:29 GMT

←Older revision		Revision as of 17:13, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [http://www.ebi.ac.uk/pdbsum/2v5q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [http://www.ebi.ac.uk/pdbsum/2v5q PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Polo kinase]]		[[Category: Polo kinase]]
	[[Category: Unidentified]]		[[Category: Unidentified]]
-	[[Category: Amstutz, P.]]	+	[[Category: Amstutz, P]]
-	[[Category: Bandeiras, T M.]]	+	[[Category: Bandeiras, T M]]
-	[[Category: Binz, H K.]]	+	[[Category: Binz, H K]]
-	[[Category: Carrondo, M A.]]	+	[[Category: Carrondo, M A]]
-	[[Category: Crusius, K.]]	+	[[Category: Crusius, K]]
-	[[Category: Donner, P.]]	+	[[Category: Donner, P]]
-	[[Category: Eberspaecher, U.]]	+	[[Category: Eberspaecher, U]]
-	[[Category: Egner, U.]]	+	[[Category: Egner, U]]
-	[[Category: Fanghaenel, J.]]	+	[[Category: Fanghaenel, J]]
-	[[Category: Gulotti-Georgieva, M.]]	+	[[Category: Gulotti-Georgieva, M]]
-	[[Category: Hillig, R C.]]	+	[[Category: Hillig, R C]]
-	[[Category: Holz, C.]]	+	[[Category: Holz, C]]
-	[[Category: Lang, C.]]	+	[[Category: Lang, C]]
-	[[Category: Matias, P M.]]	+	[[Category: Matias, P M]]
-	[[Category: Miranda, S.]]	+	[[Category: Miranda, S]]
-	[[Category: Mueller-Tiemann, B.]]	+	[[Category: Mueller-Tiemann, B]]
-	[[Category: Puetter, V.]]	+	[[Category: Puetter, V]]
-	[[Category: Schmitz, A A.P.]]	+	[[Category: Schmitz, A A.P]]
-	[[Category: Thomaz, M.]]	+	[[Category: Thomaz, M]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Design ankyrin repeat protein]]		[[Category: Design ankyrin repeat protein]]

OCA at 01:45, 1 October 2014

OCA — Wed, 01 Oct 2014 01:45:53 GMT

←Older revision		Revision as of 01:45, 1 October 2014
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-	~~{{STRUCTURE_2v5q\| PDB=2v5q \| SCENE= }}~~	+	==CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN==
-	===CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN===	+	<StructureSection load='2v5q' size='340' side='right' caption='[[2v5q]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~{{ABSTRACT_PUBMED_18391401}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2v5q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V5Q FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1q4k\|1q4k]], [[1q4o\|1q4o]], [[1umw\|1umw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polo_kinase Polo kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.21 2.7.11.21] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2v5q RCSB], [http://www.ebi.ac.uk/pdbsum/2v5q PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v5/2v5q_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	As a key regulator of mitosis, the Ser/Thr protein polo-like kinase-1 (Plk-1) is a well validated drug target in cancer therapy. In order to enable structure-guided drug design, determination of the crystal structure of the kinase domain of Plk-1 was attempted. Using a multi-parallel cloning and expression approach, a set of length variants were identified which could be expressed in large amounts from insect cells and which could be purified to high purity. However, all attempts to crystallize these constructs failed. Crystals were ultimately obtained by generating designed ankyrin-repeat proteins (DARPins) selective for Plk-1 and using them for cocrystallization. Here, the first crystal structure of the kinase domain of wild-type apo Plk-1, in complex with DARPin 3H10, is presented, underlining the power of selective DARPins as crystallization tools. The structure was refined to 2.3 A resolution and shows the active conformation of Plk-1. It broadens the basis for modelling and cocrystallization studies for drug design. The binding epitope of 3H10 is rich in arginine, glutamine and lysine residues, suggesting that the DARPin enabled crystallization by masking a surface patch which is unfavourable for crystal contact formation. Based on the packing observed in the crystal, a truncated DARPin variant was designed which showed improved binding characteristics.

-	~~==About this~~ Structure==	+	Structure of wild-type Plk-1 kinase domain in complex with a selective DARPin.,Bandeiras TM, Hillig RC, Matias PM, Eberspaecher U, Fanghanel J, Thomaz M, Miranda S, Crusius K, Putter V, Amstutz P, Gulotti-Georgieva M, Binz HK, Holz C, Schmitz AA, Lang C, Donner P, Egner U, Carrondo MA, Muller-Tiemann B Acta Crystallogr D Biol Crystallogr. 2008 Apr;64(Pt 4):339-53. Epub 2008, Mar 19. PMID:18391401<ref>PMID:18391401</ref>
-	~~[[2v5q]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens] and [http~~:~~//en~~.~~wikipedia~~.~~org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group~~=~~"xtra">PMID:018391401</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+
		+	==See Also==
		+	*[[Serine/threonine protein kinase\|Serine/threonine protein kinase]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]

OCA at 21:33, 24 March 2013

OCA — Sun, 24 Mar 2013 21:33:52 GMT

←Older revision		Revision as of 21:33, 24 March 2013
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-	[[Image:2v5q.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_2v5q", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2v5q\| PDB=2v5q \| SCENE= }}		{{STRUCTURE_2v5q\| PDB=2v5q \| SCENE= }}
-
	===CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN===		===CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18391401}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18391401 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18391401}}		{{ABSTRACT_PUBMED_18391401}}

Line 22:		Line 7:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:018391401</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018391401</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]

OCA: Protected "2v5q" [edit=sysop:move=sysop]

OCA — Wed, 24 Aug 2011 06:50:00 GMT

Protected "2v5q" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:50, 24 August 2011

OCA at 06:49, 24 August 2011

OCA — Wed, 24 Aug 2011 06:49:58 GMT

←Older revision		Revision as of 06:49, 24 August 2011
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-	{{Seed}}
	[[Image:2v5q.png\|left\|200px]]		[[Image:2v5q.png\|left\|200px]]

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	===CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN===		===CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18391401}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18391401 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18391401}}

	==About this Structure==		==About this Structure==
-	~~2V5Q~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA].	+	[[2v5q]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:018391401</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]
Line 47:		Line 55:
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Transferase complex]]		[[Category: Transferase complex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 03:17:03 2009''

OCA at 01:17, 17 February 2009

OCA — Tue, 17 Feb 2009 01:17:04 GMT

←Older revision		Revision as of 01:17, 17 February 2009
Line 1:		Line 1:
-	[[Image:2v5q.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2v5q.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2v5q\| PDB=2v5q \| SCENE= }}		{{STRUCTURE_2v5q\| PDB=2v5q \| SCENE= }}

-	~~'''~~CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN~~'''~~	+	===CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN===


	==About this Structure==		==About this Structure==
-	2V5Q is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA].	+	2V5Q is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V5Q OCA].
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Polo kinase]]		[[Category: Polo kinase]]
-	[[Category: Single protein]]
	[[Category: Unidentified]]		[[Category: Unidentified]]
	[[Category: Amstutz, P.]]		[[Category: Amstutz, P.]]
Line 47:		Line 47:
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Transferase complex]]		[[Category: Transferase complex]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 18:13~~:17 ~~2008~~''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 03:17:03 2009''