2vfk - Revision history

OCA at 07:07, 1 May 2024

OCA — Wed, 01 May 2024 07:07:18 GMT

←Older revision		Revision as of 07:07, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='2vfk' size='340' side='right'caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2vfk' size='340' side='right'caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VFK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VFK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2vfl\|2vfl]], [[2vfy\|2vfy]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [https://pdbe.org/2vfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [https://www.ebi.ac.uk/pdbsum/2vfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [https://pdbe.org/2vfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [https://www.ebi.ac.uk/pdbsum/2vfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfk ProSAT]</span></td></tr>
	</table>		</table>
Line 18:		Line 18:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vfk ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vfk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Protein kinase A anchoring proteins (AKAPs), defined by their capacity to target the cAMP-dependent protein kinase to distinct subcellular locations, function as molecular scaffolds mediating the assembly of multicomponent complexes to integrate and organise multiple signalling events. Despite their central importance in regulating cellular processes, little is known regarding their diverse structures and molecular mechanisms. Here, using bioinformatics and X-ray crystallography, we define a central domain of AKAP18 delta (AKAP18(CD)) as a member of the 2H phosphoesterase family. The domain features two conserved His-x-Thr motifs positioned at the base of a groove located between two lobes related by pseudo 2-fold symmetry. Nucleotide co-crystallisation screening revealed that this groove binds specifically to adenosine 5'-monophosphate (5'AMP) and cytosine 5'-monophosphate (5'CMP), with the affinity constant for AMP in the physiological concentration range. This is the first example of an AKAP capable of binding a small molecule. Our data generate two functional hypotheses for the AKAP18 central domain. It may act as a phosphoesterase, although we did not identify a substrate, or as an AMP sensor with the potential to couple intracellular AMP levels to PKA signalling events.
-
-	AKAP18 contains a phosphoesterase domain that binds AMP.,Gold MG, Smith FD, Scott JD, Barford D J Mol Biol. 2008 Feb 1;375(5):1329-43. Epub 2007 Nov 22. PMID:18082768<ref>PMID:18082768</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2vfk" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barford, D]]	+	[[Category: Barford D]]
-	[[Category: Gold~~, M G~~]]	+	[[Category: Gold MG]]
-	[[Category: Scott~~, J D~~]]	+	[[Category: Scott JD]]
-	[[Category: Smith~~, F D]]~~	+	[[Category: Smith FD]]
-	~~[[Category: Apo]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 11:36, 30 March 2022

OCA — Wed, 30 Mar 2022 11:36:05 GMT

←Older revision		Revision as of 11:36, 30 March 2022
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	<StructureSection load='2vfk' size='340' side='right'caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2vfk' size='340' side='right'caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2VFK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VFK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vfl\|2vfl]], [[2vfy\|2vfy]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vfl\|2vfl]], [[2vfy\|2vfy]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [~~http~~://pdbe.org/2vfk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2vfk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [https://pdbe.org/2vfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [https://www.ebi.ac.uk/pdbsum/2vfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:51, 25 June 2020

OCA — Thu, 25 Jun 2020 07:51:32 GMT

←Older revision		Revision as of 07:51, 25 June 2020
Line 1:		Line 1:

	==AKAP18 delta central domain - AMP==		==AKAP18 delta central domain - AMP==
-	<StructureSection load='2vfk' size='340' side='right' caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='2vfk' size='340' side='right'caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2VFK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VFK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vfl\|2vfl]], [[2vfy\|2vfy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vfl\|2vfl]], [[2vfy\|2vfy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [http://pdbe.org/2vfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [http://www.ebi.ac.uk/pdbsum/2vfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [http://pdbe.org/2vfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [http://www.ebi.ac.uk/pdbsum/2vfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Gold, M G]]		[[Category: Gold, M G]]

OCA at 20:57, 19 September 2018

OCA — Wed, 19 Sep 2018 20:57:19 GMT

←Older revision		Revision as of 20:57, 19 September 2018
Line 1:		Line 1:

-	==AKAP18 ~~DELTA CENTRAL DOMAIN~~ - AMP==	+	==AKAP18 delta central domain - AMP==
	<StructureSection load='2vfk' size='340' side='right' caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2vfk' size='340' side='right' caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vf/2vfk_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vf/2vfk_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:05, 11 August 2016

OCA — Thu, 11 Aug 2016 11:05:16 GMT

←Older revision		Revision as of 11:05, 11 August 2016
Line 1:		Line 1:
		+
	==AKAP18 DELTA CENTRAL DOMAIN - AMP==		==AKAP18 DELTA CENTRAL DOMAIN - AMP==
	<StructureSection load='2vfk' size='340' side='right' caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2vfk' size='340' side='right' caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VFK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VFK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vfl\|2vfl]], [[2vfy\|2vfy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vfl\|2vfl]], [[2vfy\|2vfy]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [http://www.ebi.ac.uk/pdbsum/2vfk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [http://pdbe.org/2vfk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [http://www.ebi.ac.uk/pdbsum/2vfk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vfk ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vfk ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2vfk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Gold, M G]]		[[Category: Gold, M G]]

OCA at 13:44, 19 January 2015

OCA — Mon, 19 Jan 2015 13:44:40 GMT

←Older revision		Revision as of 13:44, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VFK FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VFK FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vfl\|2vfl]], [[2vfy\|2vfy]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vfl\|2vfl]], [[2vfy\|2vfy]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [http://www.ebi.ac.uk/pdbsum/2vfk PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [http://www.ebi.ac.uk/pdbsum/2vfk PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Barford, D.]]	+	[[Category: Barford, D]]
-	[[Category: Gold, M G.]]	+	[[Category: Gold, M G]]
-	[[Category: Scott, J D.]]	+	[[Category: Scott, J D]]
-	[[Category: Smith, F D.]]	+	[[Category: Smith, F D]]
	[[Category: Apo]]		[[Category: Apo]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 01:29, 1 October 2014

OCA — Wed, 01 Oct 2014 01:29:32 GMT

←Older revision		Revision as of 01:29, 1 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2vfk.~~png~~\|~~left~~\|200px]]	+	==AKAP18 DELTA CENTRAL DOMAIN - AMP==
		+	<StructureSection load='2vfk' size='340' side='right' caption='[[2vfk]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2vfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VFK FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vfl\|2vfl]], [[2vfy\|2vfy]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vfk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vfk RCSB], [http://www.ebi.ac.uk/pdbsum/2vfk PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vf/2vfk_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein kinase A anchoring proteins (AKAPs), defined by their capacity to target the cAMP-dependent protein kinase to distinct subcellular locations, function as molecular scaffolds mediating the assembly of multicomponent complexes to integrate and organise multiple signalling events. Despite their central importance in regulating cellular processes, little is known regarding their diverse structures and molecular mechanisms. Here, using bioinformatics and X-ray crystallography, we define a central domain of AKAP18 delta (AKAP18(CD)) as a member of the 2H phosphoesterase family. The domain features two conserved His-x-Thr motifs positioned at the base of a groove located between two lobes related by pseudo 2-fold symmetry. Nucleotide co-crystallisation screening revealed that this groove binds specifically to adenosine 5'-monophosphate (5'AMP) and cytosine 5'-monophosphate (5'CMP), with the affinity constant for AMP in the physiological concentration range. This is the first example of an AKAP capable of binding a small molecule. Our data generate two functional hypotheses for the AKAP18 central domain. It may act as a phosphoesterase, although we did not identify a substrate, or as an AMP sensor with the potential to couple intracellular AMP levels to PKA signalling events.

-	~~{{STRUCTURE_2vfk\| PDB=2vfk \| SCENE= }}~~	+	AKAP18 contains a phosphoesterase domain that binds AMP.,Gold MG, Smith FD, Scott JD, Barford D J Mol Biol. 2008 Feb 1;375(5):1329-43. Epub 2007 Nov 22. PMID:18082768<ref>PMID:18082768</ref>

-	~~===AKAP18 DELTA CENTRAL DOMAIN - AMP===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18082768}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2vfk]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018082768<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]

OCA: Protected "2vfk" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 20:52:50 GMT

Protected "2vfk" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:52, 7 January 2013

OCA at 20:52, 7 January 2013

OCA — Mon, 07 Jan 2013 20:52:47 GMT

←Older revision		Revision as of 20:52, 7 January 2013
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-	{{Seed}}
	[[Image:2vfk.png\|left\|200px]]		[[Image:2vfk.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2vfk", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2vfk\| PDB=2vfk \| SCENE= }}		{{STRUCTURE_2vfk\| PDB=2vfk \| SCENE= }}

-	===AKAP18 DELTA CENTRAL DOMAIN- AMP===	+	===AKAP18 DELTA CENTRAL DOMAIN - AMP===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18082768}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18082768 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_18082768}}		{{ABSTRACT_PUBMED_18082768}}

	==About this Structure==		==About this Structure==
-	~~2VFK~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA].	+	[[2vfk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18082768~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018082768</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]
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	[[Category: Apo]]		[[Category: Apo]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 23:59:09 2009''

OCA at 21:59, 17 February 2009

OCA — Tue, 17 Feb 2009 21:59:11 GMT

←Older revision		Revision as of 21:59, 17 February 2009
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	==About this Structure==		==About this Structure==
-	2VFK is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA].	+	2VFK is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VFK OCA].

	==Reference==		==Reference==
-	~~AKAP18 contains a phosphoesterase domain that binds AMP., Gold MG, Smith FD, Scott JD, Barford D, J Mol Biol. 2008 Feb 1;375(5):1329-43. Epub 2007 Nov 22.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18082768 18082768]~~	+	<ref group="xtra">PMID:18082768</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]
	[[Category: Gold, M G.]]		[[Category: Gold, M G.]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 07~~:16:~~08 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 23:59:09 2009''