2vua - Revision history

OCA at 15:32, 13 December 2023

OCA — Wed, 13 Dec 2023 15:32:03 GMT

←Older revision		Revision as of 15:32, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2vua' size='340' side='right'caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2vua' size='340' side='right'caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VUA FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[3bta\|3bta]]~~, [[~~1uee~~\|~~1uee~~]]~~, [[2vu9\|2vu9]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en~~.~~wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [https://pdbe.org/2vua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [https://www.ebi.ac.uk/pdbsum/2vua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vua ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [https://pdbe.org/2vua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [https://www.ebi.ac.uk/pdbsum/2vua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vua ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BXA1_CLOBH BXA1_CLOBH] Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-\|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus botulinus van ermengem 1896]]~~	+	[[Category: Clostridium botulinum]]
-	~~[[Category: Bontoxilysin~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dupuy, J]]	+	[[Category: Dupuy J]]
-	[[Category: Stenmark, P]]	+	[[Category: Stenmark P]]
-	[[Category: Stevens~~, R C]]~~	+	[[Category: Stevens RC]]
-	~~[[Category: Ganglioside]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Neurotoxin]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Receptor]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 11:46, 30 March 2022

OCA — Wed, 30 Mar 2022 11:46:23 GMT

←Older revision		Revision as of 11:46, 30 March 2022
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	<StructureSection load='2vua' size='340' side='right'caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2vua' size='340' side='right'caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2VUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VUA FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [~~http~~://pdbe.org/2vua PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2vua PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2vua ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [https://pdbe.org/2vua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [https://www.ebi.ac.uk/pdbsum/2vua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vua ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:32, 25 June 2020

OCA — Thu, 25 Jun 2020 08:32:08 GMT

←Older revision		Revision as of 08:32, 25 June 2020
Line 1:		Line 1:

	==Crystal Structure of the Botulinum Neurotoxin Serotype A binding domain==		==Crystal Structure of the Botulinum Neurotoxin Serotype A binding domain==
-	<StructureSection load='2vua' size='340' side='right' caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='2vua' size='340' side='right'caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2VUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VUA FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [http://pdbe.org/2vua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [http://www.ebi.ac.uk/pdbsum/2vua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vua ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [http://pdbe.org/2vua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [http://www.ebi.ac.uk/pdbsum/2vua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vua ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]	+	*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	[[Category: Bacillus botulinus van ermengem 1896]]		[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
		+	[[Category: Large Structures]]
	[[Category: Dupuy, J]]		[[Category: Dupuy, J]]
	[[Category: Stenmark, P]]		[[Category: Stenmark, P]]

OCA at 21:22, 19 September 2018

OCA — Wed, 19 Sep 2018 21:22:45 GMT

←Older revision		Revision as of 21:22, 19 September 2018
Line 1:		Line 1:

-	==~~CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE~~ A ~~BINDING DOMAIN~~==	+	==Crystal Structure of the Botulinum Neurotoxin Serotype A binding domain==
	<StructureSection load='2vua' size='340' side='right' caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2vua' size='340' side='right' caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/2vua_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/2vua_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 20:22, 11 August 2016

OCA — Thu, 11 Aug 2016 20:22:01 GMT

←Older revision		Revision as of 20:22, 11 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE A BINDING DOMAIN==		==CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE A BINDING DOMAIN==
	<StructureSection load='2vua' size='340' side='right' caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2vua' size='340' side='right' caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VUA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VUA FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [http://www.ebi.ac.uk/pdbsum/2vua PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [http://pdbe.org/2vua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [http://www.ebi.ac.uk/pdbsum/2vua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vua ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vua ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2vua" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
-	[[Category: Clostridium botulinum]]
	[[Category: Dupuy, J]]		[[Category: Dupuy, J]]
	[[Category: Stenmark, P]]		[[Category: Stenmark, P]]

OCA at 17:07, 19 January 2015

OCA — Mon, 19 Jan 2015 17:07:12 GMT

←Older revision		Revision as of 17:07, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VUA FirstGlance]. <br>		<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VUA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [http://www.ebi.ac.uk/pdbsum/2vua PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [http://www.ebi.ac.uk/pdbsum/2vua PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 34:
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
-	[[Category: Dupuy, J.]]	+	[[Category: Dupuy, J]]
-	[[Category: Stenmark, P.]]	+	[[Category: Stenmark, P]]
-	[[Category: Stevens, R C.]]	+	[[Category: Stevens, R C]]
	[[Category: Ganglioside]]		[[Category: Ganglioside]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 01:43, 1 October 2014

OCA — Wed, 01 Oct 2014 01:43:02 GMT

←Older revision		Revision as of 01:43, 1 October 2014
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-	[[~~Image:~~2vua.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE A BINDING DOMAIN==
		+	<StructureSection load='2vua' size='340' side='right' caption='[[2vua]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2vua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VUA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bta\|3bta]], [[1uee\|1uee]], [[2vu9\|2vu9]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vua OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vua RCSB], [http://www.ebi.ac.uk/pdbsum/2vua PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/2vua_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Botulinum neurotoxins have a very high affinity and specificity for their target cells requiring two different co-receptors located on the neuronal cell surface. Different toxin serotypes have different protein receptors; yet, most share a common ganglioside co-receptor, GT1b. We determined the crystal structure of the botulinum neurotoxin serotype A binding domain (residues 873-1297) alone and in complex with a GT1b analog at 1.7 A and 1.6 A, respectively. The ganglioside GT1b forms several key hydrogen bonds to conserved residues and binds in a shallow groove lined by Tryptophan 1266. GT1b binding does not induce any large structural changes in the toxin; therefore, it is unlikely that allosteric effects play a major role in the dual receptor recognition. Together with the previously published structures of botulinum neurotoxin serotype B in complex with its protein co-receptor, we can now generate a detailed model of botulinum neurotoxin's interaction with the neuronal cell surface. The two branches of the GT1b polysaccharide, together with the protein receptor site, impose strict geometric constraints on the mode of interaction with the membrane surface and strongly support a model where one end of the 100 A long translocation domain helix bundle swing into contact with the membrane, initiating the membrane anchoring event.

-	~~{{STRUCTURE_2vua\| PDB=2vua \| SCENE= }}~~	+	Crystal structure of botulinum neurotoxin type A in complex with the cell surface co-receptor GT1b-insight into the toxin-neuron interaction.,Stenmark P, Dupuy J, Imamura A, Kiso M, Stevens RC PLoS Pathog. 2008 Aug 15;4(8):e1000129. PMID:18704164<ref>PMID:18704164</ref>

-	~~===CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE A BINDING DOMAIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18704164}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2vua]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Clostridium_botulinum Clostridium botulinum]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018704164<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]

OCA: Protected "2vua" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 22:35:30 GMT

Protected "2vua" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:35, 7 January 2013

OCA at 22:35, 7 January 2013

OCA — Mon, 07 Jan 2013 22:35:26 GMT

←Older revision		Revision as of 22:35, 7 January 2013
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-	{{Seed}}
	[[Image:2vua.png\|left\|200px]]		[[Image:2vua.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2vua", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2vua\| PDB=2vua \| SCENE= }}		{{STRUCTURE_2vua\| PDB=2vua \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE A BINDING DOMAIN===		===CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE A BINDING DOMAIN===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18704164}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18704164 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18704164}}		{{ABSTRACT_PUBMED_18704164}}

	==About this Structure==		==About this Structure==
-	~~2VUA~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA].	+	[[2vua]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18704164~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018704164</ref><references group="xtra"/>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
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	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Toxin]]		[[Category: Toxin]]
-	[[Category: Zinc]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:33:38 2009''

OCA at 07:33, 18 February 2009

OCA — Wed, 18 Feb 2009 07:33:39 GMT

←Older revision		Revision as of 07:33, 18 February 2009
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	{{Seed}}		{{Seed}}
-	[[Image:2vua.~~jpg~~\|left\|200px]]	+	[[Image:2vua.png\|left\|200px]]

	<!--		<!--
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	==About this Structure==		==About this Structure==
-	2VUA is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA].	+	2VUA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VUA OCA].

	==Reference==		==Reference==
-	Crystal structure of botulinum neurotoxin type A in complex with the cell surface co-receptor GT1b-insight into the toxin-neuron interaction., Stenmark P, Dupuy J, Imamura A, Kiso M, Stevens RC, PLoS Pathog. 2008 Aug 15;4(8):e1000129. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18704164 18704164]~~	+	<ref group="xtra">PMID:18704164</ref><references group="xtra"/>
	[[Category: Bontoxilysin]]		[[Category: Bontoxilysin]]
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
-	[[Category: Single protein]]
	[[Category: Dupuy, J.]]		[[Category: Dupuy, J.]]
	[[Category: Stenmark, P.]]		[[Category: Stenmark, P.]]
Line 42:		Line 41:
	[[Category: Zinc]]		[[Category: Zinc]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Aug 27 11~~:13:~~03 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:33:38 2009''