2vxr - Revision history

OCA at 15:36, 13 December 2023

OCA — Wed, 13 Dec 2023 15:36:30 GMT

←Older revision		Revision as of 15:36, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2vxr' size='340' side='right'caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2vxr' size='340' side='right'caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VXR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VXR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height~~: ~~3em;~~'>~~[[1zb7\|1zb7]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [https://pdbe.org/2vxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [https://www.ebi.ac.uk/pdbsum/2vxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vxr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [https://pdbe.org/2vxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [https://www.ebi.ac.uk/pdbsum/2vxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vxr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BXG_CLOBO BXG_CLOBO] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus botulinus van ermengem 1896~~]]	+	[[Category: Clostridium botulinum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dupuy, J]]	+	[[Category: Dupuy J]]
-	[[Category: Stenmark, P]]	+	[[Category: Stenmark P]]
-	[[Category: Stevens~~, R C]]~~	+	[[Category: Stevens RC]]
-	~~[[Category: Binding domain]]~~	+
-	~~[[Category: Botulinum]]~~	+
-	~~[[Category: Ganglioside]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metalloprotease]]~~	+
-	~~[[Category: Neurotoxin]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Receptor]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 11:48, 30 March 2022

OCA — Wed, 30 Mar 2022 11:48:42 GMT

←Older revision		Revision as of 11:48, 30 March 2022
Line 3:		Line 3:
	<StructureSection load='2vxr' size='340' side='right'caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2vxr' size='340' side='right'caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2VXR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VXR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zb7\|1zb7]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [~~http~~://pdbe.org/2vxr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2vxr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2vxr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [https://pdbe.org/2vxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [https://www.ebi.ac.uk/pdbsum/2vxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vxr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:40, 25 June 2020

OCA — Thu, 25 Jun 2020 08:40:31 GMT

←Older revision		Revision as of 08:40, 25 June 2020
Line 1:		Line 1:

	==Crystal Structure of the Botulinum Neurotoxin serotype G binding domain==		==Crystal Structure of the Botulinum Neurotoxin serotype G binding domain==
-	<StructureSection load='2vxr' size='340' side='right' caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='2vxr' size='340' side='right'caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2VXR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2VXR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://pdbe.org/2vxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vxr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://pdbe.org/2vxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vxr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]	+	*[[Botulinum neurotoxin 3D structures\|Botulinum neurotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus botulinus van ermengem 1896]]		[[Category: Bacillus botulinus van ermengem 1896]]
		+	[[Category: Large Structures]]
	[[Category: Dupuy, J]]		[[Category: Dupuy, J]]
	[[Category: Stenmark, P]]		[[Category: Stenmark, P]]

OCA at 21:02, 19 September 2018

OCA — Wed, 19 Sep 2018 21:02:19 GMT

←Older revision		Revision as of 21:02, 19 September 2018
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE~~ G ~~BINDING DOMAIN~~==	+
		+	==Crystal Structure of the Botulinum Neurotoxin serotype G binding domain==
	<StructureSection load='2vxr' size='340' side='right' caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2vxr' size='340' side='right' caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://pdbe.org/2vxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://pdbe.org/2vxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vxr ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vx/2vxr_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vx/2vxr_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 18:55, 7 February 2016

OCA — Sun, 07 Feb 2016 18:55:43 GMT

←Older revision		Revision as of 18:55, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='2vxr' size='340' side='right' caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2vxr' size='340' side='right' caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_botulinum Clostridium botulinum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VXR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VXR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://pdbe.org/2vxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vxr ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2vxr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Botulinum neurotoxin\|Botulinum neurotoxin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium botulinum~~]]	+	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Dupuy, J]]		[[Category: Dupuy, J]]
	[[Category: Stenmark, P]]		[[Category: Stenmark, P]]

OCA at 13:49, 19 January 2015

OCA — Mon, 19 Jan 2015 13:49:54 GMT

←Older revision		Revision as of 13:49, 19 January 2015
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
-	[[Category: Dupuy, J.]]	+	[[Category: Dupuy, J]]
-	[[Category: Stenmark, P.]]	+	[[Category: Stenmark, P]]
-	[[Category: Stevens, R C.]]	+	[[Category: Stevens, R C]]
	[[Category: Binding domain]]		[[Category: Binding domain]]
	[[Category: Botulinum]]		[[Category: Botulinum]]

OCA at 06:01, 10 October 2014

OCA — Fri, 10 Oct 2014 06:01:57 GMT

←Older revision		Revision as of 06:01, 10 October 2014
Line 2:		Line 2:
	<StructureSection load='2vxr' size='340' side='right' caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2vxr' size='340' side='right' caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. <br>	+	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VXR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 23:
	Crystal structure of the botulinum neurotoxin type G binding domain: insight into cell surface binding.,Stenmark P, Dong M, Dupuy J, Chapman ER, Stevens RC J Mol Biol. 2010 Apr 16;397(5):1287-97. Epub 2010 Feb 26. PMID:20219474<ref>PMID:20219474</ref>		Crystal structure of the botulinum neurotoxin type G binding domain: insight into cell surface binding.,Stenmark P, Dong M, Dupuy J, Chapman ER, Stevens RC J Mol Biol. 2010 Apr 16;397(5):1287-97. Epub 2010 Feb 26. PMID:20219474<ref>PMID:20219474</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 08:32, 7 May 2014

OCA — Wed, 07 May 2014 08:32:34 GMT

←Older revision		Revision as of 08:32, 7 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2vxr.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE G BINDING DOMAIN==
		+	<StructureSection load='2vxr' size='340' side='right' caption='[[2vxr]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2vxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zb7\|1zb7]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vxr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vxr RCSB], [http://www.ebi.ac.uk/pdbsum/2vxr PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vx/2vxr_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Botulinum neurotoxins (BoNTs) typically bind the neuronal cell surface via dual interactions with both protein receptors and gangliosides. We present here the 1.9-A X-ray structure of the BoNT serotype G (BoNT/G) receptor binding domain (residues 868-1297) and a detailed view of protein receptor and ganglioside binding regions. The ganglioside binding motif (SxWY) has a conserved structure compared to the corresponding regions in BoNT serotype A and BoNT serotype B (BoNT/B), but several features of interactions with the hydrophilic face of the ganglioside are absent at the opposite side of the motif in the BoNT/G ganglioside binding cleft. This may significantly reduce the affinity between BoNT/G and gangliosides. BoNT/G and BoNT/B share the protein receptor synaptotagmin (Syt) I/II. The Syt binding site has a conserved hydrophobic plateau located centrally in the proposed protein receptor binding interface (Tyr1189, Phe1202, Ala1204, Pro1205, and Phe1212). Interestingly, only 5 of 14 residues that are important for binding between Syt-II and BoNT/B are conserved in BoNT/G, suggesting that the means by which BoNT/G and BoNT/B bind Syt diverges more than previously appreciated. Indeed, substitution of Syt-II Phe47 and Phe55 with alanine residues had little effect on the binding of BoNT/G, but strongly reduced the binding of BoNT/B. Furthermore, an extended solvent-exposed hydrophobic loop, located between the Syt binding site and the ganglioside binding cleft, may serve as a third membrane association and binding element to contribute to high-affinity binding to the neuronal membrane. While BoNT/G and BoNT/B are homologous to each other and both utilize Syt-I/Syt-II as their protein receptor, the precise means by which these two toxin serotypes bind to Syt appears surprisingly divergent.

-	~~<!--~~	+	Crystal structure of the botulinum neurotoxin type G binding domain: insight into cell surface binding.,Stenmark P, Dong M, Dupuy J, Chapman ER, Stevens RC J Mol Biol. 2010 Apr 16;397(5):1287-97. Epub 2010 Feb 26. PMID:20219474<ref>PMID:20219474</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2vxr"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2vxr\| PDB=2vxr \| SCENE= }}~~	+

-	~~===CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE G BINDING DOMAIN===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_20219474}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 20219474 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_20219474}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2vxr]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020219474<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Dupuy, J.]]		[[Category: Dupuy, J.]]

OCA: Protected "2vxr" [edit=sysop:move=sysop]

OCA — Wed, 20 Jul 2011 05:26:01 GMT

Protected "2vxr" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:26, 20 July 2011

OCA at 05:25, 20 July 2011

OCA — Wed, 20 Jul 2011 05:25:59 GMT

←Older revision		Revision as of 05:25, 20 July 2011
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:2vxr.png\|left\|200px]]
-	[[Image:2vxr.~~jpg~~\|left\|200px]]	+

	<!--		<!--
Line 12:		Line 11:
	===CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE G BINDING DOMAIN===		===CRYSTAL STRUCTURE OF THE BOTULINUM NEUROTOXIN SEROTYPE G BINDING DOMAIN===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20219474}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20219474 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20219474}}

	==About this Structure==		==About this Structure==
-	~~2VXR~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA].	+	[[2vxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VXR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:020219474</ref><references group="xtra"/>
	[[Category: Clostridium botulinum]]		[[Category: Clostridium botulinum]]
	[[Category: Dupuy, J.]]		[[Category: Dupuy, J.]]
Line 29:		Line 37:
	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Toxin]]		[[Category: Toxin]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 8 12:37:20 2009''