2w58 - Revision history

OCA at 10:08, 9 May 2024

OCA — Thu, 09 May 2024 10:08:15 GMT

←Older revision		Revision as of 10:08, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='2w58' size='340' side='right'caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2w58' size='340' side='right'caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Geoka Geoka~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W58 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W58 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [https://pdbe.org/2w58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [https://www.ebi.ac.uk/pdbsum/2w58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w58 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [https://pdbe.org/2w58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [https://www.ebi.ac.uk/pdbsum/2w58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w58 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5KWD0_GEOKA Q5KWD0_GEOKA]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Geoka~~]]	+	[[Category: Geobacillus kaustophilus HTA426]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hsiao~~, C D~~]]	+	[[Category: Hsiao CD]]
-	[[Category: Lo~~, Y H~~]]	+	[[Category: Lo YH]]
-	[[Category: Sun~~, Y J~~]]	+	[[Category: Sun YJ]]
-	[[Category: Tsai~~, K L]]~~	+	[[Category: Tsai KL]]
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Dnai]]~~	+
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Nucleotide-binding~~]]	+

OCA at 16:41, 3 November 2021

OCA — Wed, 03 Nov 2021 16:41:53 GMT

←Older revision		Revision as of 16:41, 3 November 2021
Line 3:		Line 3:
	<StructureSection load='2w58' size='340' side='right'caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2w58' size='340' side='right'caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Geoka Geoka]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2W58 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geoka Geoka]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W58 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [~~http~~://pdbe.org/2w58 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2w58 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2w58 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [https://pdbe.org/2w58 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [https://www.ebi.ac.uk/pdbsum/2w58 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w58 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 06:11, 16 October 2019

OCA — Wed, 16 Oct 2019 06:11:40 GMT

←Older revision		Revision as of 06:11, 16 October 2019
Line 1:		Line 1:

	==Crystal Structure of the DnaI==		==Crystal Structure of the DnaI==
-	<StructureSection load='2w58' size='340' side='right' caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='2w58' size='340' side='right'caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geoka Geoka]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W58 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geoka Geoka]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W58 FirstGlance]. <br>
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Geoka]]		[[Category: Geoka]]
		+	[[Category: Large Structures]]
	[[Category: Hsiao, C D]]		[[Category: Hsiao, C D]]
	[[Category: Lo, Y H]]		[[Category: Lo, Y H]]

OCA at 09:26, 26 September 2018

OCA — Wed, 26 Sep 2018 09:26:03 GMT

←Older revision		Revision as of 09:26, 26 September 2018
Line 11:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w58_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w58_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:24, 11 August 2016

OCA — Thu, 11 Aug 2016 16:24:58 GMT

←Older revision		Revision as of 16:24, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of the DnaI==		==Crystal Structure of the DnaI==
	<StructureSection load='2w58' size='340' side='right' caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='2w58' size='340' side='right' caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W58 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geoka Geoka]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W58 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [http://www.ebi.ac.uk/pdbsum/2w58 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [http://pdbe.org/2w58 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [http://www.ebi.ac.uk/pdbsum/2w58 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w58 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 14:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w58 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2w58" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Geobacillus kaustophilus hta426~~]]	+	[[Category: Geoka]]
	[[Category: Hsiao, C D]]		[[Category: Hsiao, C D]]
	[[Category: Lo, Y H]]		[[Category: Lo, Y H]]

OCA at 16:51, 19 January 2015

OCA — Mon, 19 Jan 2015 16:51:25 GMT

←Older revision		Revision as of 16:51, 19 January 2015
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Geobacillus kaustophilus hta426]]		[[Category: Geobacillus kaustophilus hta426]]
-	[[Category: Hsiao, C D.]]	+	[[Category: Hsiao, C D]]
-	[[Category: Lo, Y H.]]	+	[[Category: Lo, Y H]]
-	[[Category: Sun, Y J.]]	+	[[Category: Sun, Y J]]
-	[[Category: Tsai, K L.]]	+	[[Category: Tsai, K L]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Dnai]]		[[Category: Dnai]]

OCA at 06:03, 10 October 2014

OCA — Fri, 10 Oct 2014 06:03:51 GMT

←Older revision		Revision as of 06:03, 10 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W58 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W58 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [http://www.ebi.ac.uk/pdbsum/2w58 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [http://www.ebi.ac.uk/pdbsum/2w58 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:03, 9 June 2014

OCA — Mon, 09 Jun 2014 07:03:14 GMT

←Older revision		Revision as of 07:03, 9 June 2014
Line 1:		Line 1:
-	[[~~Image~~:2w58.~~png\|left~~\|200px]]	+	==Crystal Structure of the DnaI==
		+	<StructureSection load='2w58' size='340' side='right' caption='[[2w58]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2w58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W58 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w58 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w58 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w58 RCSB], [http://www.ebi.ac.uk/pdbsum/2w58 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w58_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Helicase loading factors are thought to transfer the hexameric ring-shaped helicases onto the replication fork during DNA replication. However, the mechanism of helicase transfer onto DNA remains unclear. In Bacillus subtilis, the protein DnaI, which belongs to the AAA+ family of ATPases, is responsible for delivering the hexameric helicase DnaC onto DNA. Here we investigated the interaction between DnaC and DnaI from Geobacillus kaustophilus HTA426 (GkDnaC and GkDnaI, respectively) and determined that GkDnaI forms a stable complex with GkDnaC with an apparent stoichiometry of GkDnaC(6)-GkDnaI(6) in the absence of ATP. Surface plasmon resonance analysis indicated that GkDnaI facilitates loading of GkDnaC onto single-stranded DNA (ssDNA) and supports complex formation with ssDNA in the presence of ATP. Additionally, the GkDnaI C-terminal AAA+ domain alone could bind ssDNA, and binding was modulated by nucleotides. We also determined the crystal structure of the C-terminal AAA+ domain of GkDnaI in complex with ADP at 2.5 A resolution. The structure not only delineates the binding of ADP in the expected Walker A and B motifs but also reveals a positively charged region that may be involved in ssDNA binding. These findings provide insight into the mechanism of replicative helicase loading onto ssDNA.

-	~~{{STRUCTURE_2w58\| PDB=2w58 \| SCENE= }}~~	+	Molecular interplay between the replicative helicase DnaC and its loader protein DnaI from Geobacillus kaustophilus.,Tsai KL, Lo YH, Sun YJ, Hsiao CD J Mol Biol. 2009 Nov 13;393(5):1056-69. Epub 2009 Sep 8. PMID:19744498<ref>PMID:19744498</ref>

-	~~===Crystal Structure~~ of the ~~DnaI===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19744498}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2w58]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019744498<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Geobacillus kaustophilus hta426]]		[[Category: Geobacillus kaustophilus hta426]]
	[[Category: Hsiao, C D.]]		[[Category: Hsiao, C D.]]

OCA: Protected "2w58" [edit=sysop:move=sysop]

OCA — Wed, 22 Aug 2012 05:11:35 GMT

Protected "2w58" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 22 August 2012

OCA at 05:11, 22 August 2012

OCA — Wed, 22 Aug 2012 05:11:33 GMT

←Older revision		Revision as of 05:11, 22 August 2012
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-	~~{{Seed}}~~	+	[[Image:2w58.png\|left\|200px]]
-	[[Image:2w58.~~jpg~~\|left\|200px]]	+

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	{{STRUCTURE_2w58\| PDB=2w58 \| SCENE= }}		{{STRUCTURE_2w58\| PDB=2w58 \| SCENE= }}

-	===~~CRYSTAL STRUCTURE OF THE DNAI~~===	+	===Crystal Structure of the DnaI===

		+	{{ABSTRACT_PUBMED_19744498}}

	==About this Structure==		==About this Structure==
-	~~2W58~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA].	+	[[2w58]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W58 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:019744498</ref><references group="xtra"/>
	[[Category: Geobacillus kaustophilus hta426]]		[[Category: Geobacillus kaustophilus hta426]]
	[[Category: Hsiao, C D.]]		[[Category: Hsiao, C D.]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Nucleotide-binding]]		[[Category: Nucleotide-binding]]
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-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 23 10:02:38 2009''