2w5w - Revision history

OCA at 10:58, 6 April 2022

OCA — Wed, 06 Apr 2022 10:58:04 GMT

←Older revision		Revision as of 10:58, 6 April 2022
Line 3:		Line 3:
	<StructureSection load='2w5w' size='340' side='right'caption='[[2w5w]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='2w5w' size='340' side='right'caption='[[2w5w]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w5w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2W5W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w5w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W5W FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [~~http~~://pdbe.org/2w5w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2w5w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2w5w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [https://pdbe.org/2w5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [https://www.ebi.ac.uk/pdbsum/2w5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w5w ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	==See Also==		==See Also==
	*[[Alkaline phosphatase 3D structures\|Alkaline phosphatase 3D structures]]		*[[Alkaline phosphatase 3D structures\|Alkaline phosphatase 3D structures]]
-	*[[User:Andrzej Lyskowski\|User:Andrzej Lyskowski]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:14, 1 July 2020

OCA — Wed, 01 Jul 2020 08:14:13 GMT

←Older revision		Revision as of 08:14, 1 July 2020
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	==Structure of TAB5 alkaline phosphatase mutant His 135 Asp with Zn bound in the M3 site.==		==Structure of TAB5 alkaline phosphatase mutant His 135 Asp with Zn bound in the M3 site.==
-	<StructureSection load='2w5w' size='340' side='right' caption='[[2w5w]], [[Resolution\|resolution]] 1.79Å' scene=''>	+	<StructureSection load='2w5w' size='340' side='right'caption='[[2w5w]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2W5W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W5W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://pdbe.org/2w5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w5w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://pdbe.org/2w5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w5w ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Alkaline phosphatase\|Alkaline phosphatase]]	+	*[[Alkaline phosphatase 3D structures\|Alkaline phosphatase 3D structures]]
	*[[User:Andrzej Lyskowski\|User:Andrzej Lyskowski]]		*[[User:Andrzej Lyskowski\|User:Andrzej Lyskowski]]
	== References ==		== References ==
Line 39:		Line 39:
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
	[[Category: Antarctic bacterium tab5]]		[[Category: Antarctic bacterium tab5]]
		+	[[Category: Large Structures]]
	[[Category: Bouriotis, V]]		[[Category: Bouriotis, V]]
	[[Category: Feller, G]]		[[Category: Feller, G]]

OCA at 08:52, 26 September 2018

OCA — Wed, 26 Sep 2018 08:52:02 GMT

←Older revision		Revision as of 08:52, 26 September 2018
Line 1:		Line 1:
-	==~~STRUCTURE OF~~ TAB5 ~~ALKALINE PHOSPHATASE MUTANT HIS~~ 135 ~~ASP WITH ZN BOUND IN THE~~ M3 ~~SITE~~.==	+
		+	==Structure of TAB5 alkaline phosphatase mutant His 135 Asp with Zn bound in the M3 site.==
	<StructureSection load='2w5w' size='340' side='right' caption='[[2w5w]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='2w5w' size='340' side='right' caption='[[2w5w]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 7:		Line 8:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://pdbe.org/2w5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://pdbe.org/2w5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w5w ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w5w_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w5w_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:03, 10 February 2016

OCA — Wed, 10 Feb 2016 04:03:20 GMT

←Older revision		Revision as of 04:03, 10 February 2016
Line 7:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://pdbe.org/2w5w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w5w ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2w5w" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 14:44, 19 January 2015

OCA — Mon, 19 Jan 2015 14:44:21 GMT

←Older revision		Revision as of 14:44, 19 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2w5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W5W FirstGlance]. <br>		<table><tr><td colspan='2'>[[2w5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W5W FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 37:
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
	[[Category: Antarctic bacterium tab5]]		[[Category: Antarctic bacterium tab5]]
-	[[Category: Bouriotis, V.]]	+	[[Category: Bouriotis, V]]
-	[[Category: Feller, G.]]	+	[[Category: Feller, G]]
-	[[Category: Guthrie, E.]]	+	[[Category: Guthrie, E]]
-	[[Category: Heikinheimo, P.]]	+	[[Category: Heikinheimo, P]]
-	[[Category: Koutsioulis, D.]]	+	[[Category: Koutsioulis, D]]
-	[[Category: Lyskowski, A.]]	+	[[Category: Lyskowski, A]]
-	[[Category: Maki, S~~.]]~~	+	[[Category: Maki, S]]
-	~~[[Category: Alkaline phosphatase~~]]	+
	[[Category: Cold adaptation]]		[[Category: Cold adaptation]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Psychrophile]]		[[Category: Psychrophile]]

OCA at 01:47, 1 October 2014

OCA — Wed, 01 Oct 2014 01:47:50 GMT

←Older revision		Revision as of 01:47, 1 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2w5w\| PDB=2w5w \| SCENE= }}~~	+	==STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 ASP WITH ZN BOUND IN THE M3 SITE.==
-	===STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 ASP WITH ZN BOUND IN THE M3 SITE.===	+	<StructureSection load='2w5w' size='340' side='right' caption='[[2w5w]], [[Resolution\|resolution]] 1.79Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19916164}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2w5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W5W FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2iuc\|2iuc]], [[2w5v\|2w5v]], [[2w5x\|2w5x]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w5w RCSB], [http://www.ebi.ac.uk/pdbsum/2w5w PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w5w_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Alkaline phosphatases (APs) are commercially applied enzymes that catalyze the hydrolysis of phosphate monoesters by a reaction involving three active site metal ions. We have previously identified H135 as the key residue for controlling activity of the psychrophilic TAB5 AP (TAP). In this paper we describe three X-ray crystallographic structures on TAP variants H135E and H135D in complex with a variety of metal ions. The structural analysis is supported by thermodynamic and kinetic data. The AP catalysis essentially requires octahedral coordination in the M3 site, but stability is adjusted with the conformational freedom of the metal ion. Comparison to the mesophilic E. coli alkaline phosphatase shows differences in the charge transfer network in providing the chemically optimal metal combination for catalysis. Our results provide explanation why the TAB5 and E. coli APs respond in an opposite way to mutagenesis in their active sites. They provide a lesson on chemical fine tuning and the importance of the second coordination sphere in defining metal specificity in enzymes. Understanding the framework of alkaline phosphatase catalysis is essential in the efforts to design even more powerful tools for modern biotechnology.

-	~~==About this Structure==~~	+	Coordination sphere of the third metal site is essential to the activity and metal selectivity of alkaline phosphatases.,Koutsioulis D, Lyskowski A, Maki S, Guthrie E, Feller G, Bouriotis V, Heikinheimo P Protein Sci. 2009 Nov 13. PMID:19916164<ref>PMID:19916164</ref>
-	~~[[2w5w]]~~ is ~~a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=2W5W OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Alkaline phosphatase\|Alkaline phosphatase]]		*[[Alkaline phosphatase\|Alkaline phosphatase]]
	*[[User:Andrzej Lyskowski\|User:Andrzej Lyskowski]]		*[[User:Andrzej Lyskowski\|User:Andrzej Lyskowski]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019916164<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
	[[Category: Antarctic bacterium tab5]]		[[Category: Antarctic bacterium tab5]]

OCA at 09:40, 11 March 2013

OCA — Mon, 11 Mar 2013 09:40:38 GMT

←Older revision		Revision as of 09:40, 11 March 2013
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-	[[Image:2w5w.png\|left\|200px]]
-
	{{STRUCTURE_2w5w\| PDB=2w5w \| SCENE= }}		{{STRUCTURE_2w5w\| PDB=2w5w \| SCENE= }}
-
	===STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 ASP WITH ZN BOUND IN THE M3 SITE.===		===STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 ASP WITH ZN BOUND IN THE M3 SITE.===
-
	{{ABSTRACT_PUBMED_19916164}}		{{ABSTRACT_PUBMED_19916164}}

OCA at 16:16, 20 October 2012

OCA — Sat, 20 Oct 2012 16:16:16 GMT

←Older revision		Revision as of 16:16, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2w5w]] is a 2 chain structure ~~of [[Alkaline phosphatase]]~~ with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA].	+	[[2w5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA].

	==See Also==		==See Also==
	*[[Alkaline phosphatase\|Alkaline phosphatase]]		*[[Alkaline phosphatase\|Alkaline phosphatase]]
		+	*[[User:Andrzej Lyskowski\|User:Andrzej Lyskowski]]

	==Reference==		==Reference==

OCA: Protected "2w5w" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 21:08:18 GMT

Protected "2w5w" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:08, 26 July 2012

OCA at 21:08, 26 July 2012

OCA — Thu, 26 Jul 2012 21:08:17 GMT

←Older revision		Revision as of 21:08, 26 July 2012
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-	{{Seed}}
	[[Image:2w5w.png\|left\|200px]]		[[Image:2w5w.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2w5w", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2w5w\| PDB=2w5w \| SCENE= }}		{{STRUCTURE_2w5w\| PDB=2w5w \| SCENE= }}

	===STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 ASP WITH ZN BOUND IN THE M3 SITE.===		===STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 ASP WITH ZN BOUND IN THE M3 SITE.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19916164}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19916164 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19916164}}		{{ABSTRACT_PUBMED_19916164}}

	==About this Structure==		==About this Structure==
-	~~2W5W~~ is a 2 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA].	+	[[2w5w]] is a 2 chain structure of [[Alkaline phosphatase]] with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5W OCA].
		+
		+	==See Also==
		+	*[[Alkaline phosphatase\|Alkaline phosphatase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19916164~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019916164</ref><references group="xtra"/>
	[[Category: Alkaline phosphatase]]		[[Category: Alkaline phosphatase]]
	[[Category: Antarctic bacterium tab5]]		[[Category: Antarctic bacterium tab5]]
Line 37:		Line 28:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Psychrophile]]		[[Category: Psychrophile]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 25 09:31:33 2010''