2w9x - Revision history

OCA at 11:01, 6 April 2022

OCA — Wed, 06 Apr 2022 11:01:33 GMT

←Older revision		Revision as of 11:01, 6 April 2022
Line 3:		Line 3:
	<StructureSection load='2w9x' size='340' side='right'caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2w9x' size='340' side='right'caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2W9X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W9X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [~~http~~://pdbe.org/2w9x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2w9x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9x ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [https://pdbe.org/2w9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [https://www.ebi.ac.uk/pdbsum/2w9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9x ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:24, 1 July 2020

OCA — Wed, 01 Jul 2020 08:24:29 GMT

←Older revision		Revision as of 08:24, 1 July 2020
Line 1:		Line 1:

	==The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions==		==The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions==
-	<StructureSection load='2w9x' size='340' side='right' caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2w9x' size='340' side='right'caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2W9X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2W9X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://pdbe.org/2w9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9x ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://pdbe.org/2w9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9x ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Cellvibrio cellulosa nagy et al. 2002]]		[[Category: Cellvibrio cellulosa nagy et al. 2002]]
		+	[[Category: Large Structures]]
	[[Category: Benedita, P A]]		[[Category: Benedita, P A]]
	[[Category: Bolam, D N]]		[[Category: Bolam, D N]]

OCA at 09:03, 26 September 2018

OCA — Wed, 26 Sep 2018 09:03:31 GMT

←Older revision		Revision as of 09:03, 26 September 2018
Line 1:		Line 1:
-	==~~THE ACTIVE SITE OF A CARBOHYDRATE ESTERASE DISPLAYS DIVERGENT CATALYTIC AND NON~~-~~CATALYTIC BINDING FUNCTIONS~~==	+
		+	==The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions==
	<StructureSection load='2w9x' size='340' side='right' caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2w9x' size='340' side='right' caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://pdbe.org/2w9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://pdbe.org/2w9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w9x ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/2w9x_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/2w9x_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 14:28, 8 February 2016

OCA — Mon, 08 Feb 2016 14:28:48 GMT

←Older revision		Revision as of 14:28, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='2w9x' size='340' side='right' caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2w9x' size='340' side='right' caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cellvibrio_japonicus Cellvibrio japonicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W9X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"cellvibrio_cellulosa"_nagy_et_al._2002 "cellvibrio cellulosa" nagy et al. 2002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W9X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://pdbe.org/2w9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w9x ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2w9x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cellvibrio ~~japonicus~~]]	+	[[Category: Cellvibrio cellulosa nagy et al. 2002]]
	[[Category: Benedita, P A]]		[[Category: Benedita, P A]]
	[[Category: Bolam, D N]]		[[Category: Bolam, D N]]

OCA at 00:00, 25 December 2014

OCA — Thu, 25 Dec 2014 00:00:38 GMT

←Older revision		Revision as of 00:00, 25 December 2014
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Cellvibrio japonicus]]		[[Category: Cellvibrio japonicus]]
-	[[Category: Benedita, P A.]]	+	[[Category: Benedita, P A]]
-	[[Category: Bolam, D N.]]	+	[[Category: Bolam, D N]]
-	[[Category: Cartmell, A.]]	+	[[Category: Cartmell, A]]
-	[[Category: Davies, G J.]]	+	[[Category: Davies, G J]]
-	[[Category: Dobson, E.]]	+	[[Category: Dobson, E]]
-	[[Category: Flint, J E.]]	+	[[Category: Flint, J E]]
-	[[Category: Fontes, C M.]]	+	[[Category: Fontes, C M]]
-	[[Category: Gilbert, H J.]]	+	[[Category: Gilbert, H J]]
-	[[Category: Goyal, A.]]	+	[[Category: Goyal, A]]
-	[[Category: Izumi, A.]]	+	[[Category: Izumi, A]]
-	[[Category: Kolenova, K.]]	+	[[Category: Kolenova, K]]
-	[[Category: Money, V A.]]	+	[[Category: Money, V A]]
-	[[Category: Montanier, C.]]	+	[[Category: Montanier, C]]
-	[[Category: Morland, C.]]	+	[[Category: Morland, C]]
-	[[Category: Pires, V.]]	+	[[Category: Pires, V]]
-	[[Category: Prates, J A.]]	+	[[Category: Prates, J A]]
-	[[Category: Stalbrand, H.]]	+	[[Category: Stalbrand, H]]
-	[[Category: Topakas, E.]]	+	[[Category: Topakas, E]]
	[[Category: Acetyl xylan esterase]]		[[Category: Acetyl xylan esterase]]
	[[Category: Carbohydrate esterase family 2]]		[[Category: Carbohydrate esterase family 2]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 06:28, 10 October 2014

OCA — Fri, 10 Oct 2014 06:28:10 GMT

←Older revision		Revision as of 06:28, 10 October 2014
Line 2:		Line 2:
	<StructureSection load='2w9x' size='340' side='right' caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2w9x' size='340' side='right' caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. <br>	+	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W9X FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 23:
	The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions.,Montanier C, Money VA, Pires VM, Flint JE, Pinheiro BA, Goyal A, Prates JA, Izumi A, Stalbrand H, Morland C, Cartmell A, Kolenova K, Topakas E, Dodson EJ, Bolam DN, Davies GJ, Fontes CM, Gilbert HJ PLoS Biol. 2009 Mar 31;7(3):e71. PMID:19338387<ref>PMID:19338387</ref>		The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions.,Montanier C, Money VA, Pires VM, Flint JE, Pinheiro BA, Goyal A, Prates JA, Izumi A, Stalbrand H, Morland C, Cartmell A, Kolenova K, Topakas E, Dodson EJ, Bolam DN, Davies GJ, Fontes CM, Gilbert HJ PLoS Biol. 2009 Mar 31;7(3):e71. PMID:19338387<ref>PMID:19338387</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 08:37, 7 May 2014

OCA — Wed, 07 May 2014 08:37:23 GMT

←Older revision		Revision as of 08:37, 7 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2w9x.~~png~~\|~~left~~\|200px]]	+	==THE ACTIVE SITE OF A CARBOHYDRATE ESTERASE DISPLAYS DIVERGENT CATALYTIC AND NON-CATALYTIC BINDING FUNCTIONS==
		+	<StructureSection load='2w9x' size='340' side='right' caption='[[2w9x]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2w9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wab\|2wab]], [[2wao\|2wao]], [[2waa\|2waa]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w9x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w9x RCSB], [http://www.ebi.ac.uk/pdbsum/2w9x PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w9/2w9x_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Multifunctional proteins, which play a critical role in many biological processes, have typically evolved through the recruitment of different domains that have the required functional diversity. Thus the different activities displayed by these proteins are mediated by spatially distinct domains, consistent with the specific chemical requirements of each activity. Indeed, current evolutionary theory argues that the colocalization of diverse activities within an enzyme is likely to be a rare event, because it would compromise the existing activity of the protein. In contrast to this view, a potential example of multifunctional recruitment into a single protein domain is provided by CtCel5C-CE2, which contains an N-terminal module that displays cellulase activity and a C-terminal module, CtCE2, which exhibits a noncatalytic cellulose-binding function but also shares sequence identity with the CE2 family of esterases. Here we show that, unlike other CE2 members, the CtCE2 domain displays divergent catalytic esterase and noncatalytic carbohydrate binding functions. Intriguingly, these diverse activities are housed within the same site on the protein. Thus, a critical component of the active site of CtCE2, the catalytic Ser-His dyad, in harness with inserted aromatic residues, confers noncatalytic binding to cellulose whilst the active site of the domain retains its esterase activity. CtCE2 catalyses deacetylation of noncellulosic plant structural polysaccharides to deprotect these substrates for attack by other enzymes. Yet it also acts as a cellulose-binding domain, which promotes the activity of the appended cellulase on recalcitrant substrates. The CE2 family encapsulates the requirement for multiple activities by biocatalysts that attack challenging macromolecular substrates, including the grafting of a second, powerful and discrete noncatalytic binding functionality into the active site of an enzyme. This article provides a rare example of "gene sharing," where the introduction of a second functionality into the active site of an enzyme does not compromise the original activity of the biocatalyst.

-	~~<!--~~	+	The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions.,Montanier C, Money VA, Pires VM, Flint JE, Pinheiro BA, Goyal A, Prates JA, Izumi A, Stalbrand H, Morland C, Cartmell A, Kolenova K, Topakas E, Dodson EJ, Bolam DN, Davies GJ, Fontes CM, Gilbert HJ PLoS Biol. 2009 Mar 31;7(3):e71. PMID:19338387<ref>PMID:19338387</ref>
-	The ~~line below this paragraph~~, ~~containing "STRUCTURE_2w9x"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2w9x\| PDB=2w9x \| SCENE= }}~~	+

-	~~===THE ACTIVE SITE OF A CARBOHYDRATE ESTERASE DISPLAYS DIVERGENT CATALYTIC AND NON-CATALYTIC BINDING FUNCTIONS===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_19338387}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 19338387 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_19338387}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2w9x]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019338387<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Cellvibrio japonicus]]		[[Category: Cellvibrio japonicus]]
	[[Category: Benedita, P A.]]		[[Category: Benedita, P A.]]

OCA: Protected "2w9x" [edit=sysop:move=sysop]

OCA — Wed, 24 Aug 2011 05:17:19 GMT

Protected "2w9x" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 24 August 2011

OCA at 05:17, 24 August 2011

OCA — Wed, 24 Aug 2011 05:17:18 GMT

←Older revision		Revision as of 05:17, 24 August 2011
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-	{{Seed}}
	[[Image:2w9x.png\|left\|200px]]		[[Image:2w9x.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~2W9X~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA].	+	[[2w9x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19338387~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019338387</ref><references group="xtra"/>
	[[Category: Cellvibrio japonicus]]		[[Category: Cellvibrio japonicus]]
	[[Category: Benedita, P A.]]		[[Category: Benedita, P A.]]
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	[[Category: Carbohydrate esterase family 2]]		[[Category: Carbohydrate esterase family 2]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 15 09:24:11 2009''

OCA at 06:24, 15 April 2009

OCA — Wed, 15 Apr 2009 06:24:11 GMT

←Older revision		Revision as of 06:24, 15 April 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:2w9x.~~jpg~~\|left\|200px]]	+	[[Image:2w9x.png\|left\|200px]]

	<!--		<!--
Line 12:		Line 12:
	===THE ACTIVE SITE OF A CARBOHYDRATE ESTERASE DISPLAYS DIVERGENT CATALYTIC AND NON-CATALYTIC BINDING FUNCTIONS===		===THE ACTIVE SITE OF A CARBOHYDRATE ESTERASE DISPLAYS DIVERGENT CATALYTIC AND NON-CATALYTIC BINDING FUNCTIONS===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19338387}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19338387 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19338387}}

	==About this Structure==		==About this Structure==
	2W9X is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA].		2W9X is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W9X OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19338387</ref><references group="xtra"/>
	[[Category: Cellvibrio japonicus]]		[[Category: Cellvibrio japonicus]]
	[[Category: Benedita, P A.]]		[[Category: Benedita, P A.]]
Line 23:		Line 32:
	[[Category: Flint, J E.]]		[[Category: Flint, J E.]]
	[[Category: Fontes, C M.]]		[[Category: Fontes, C M.]]
-	[[Category: Gilbert, H.]]	+	[[Category: Gilbert, H J.]]
	[[Category: Goyal, A.]]		[[Category: Goyal, A.]]
	[[Category: Izumi, A.]]		[[Category: Izumi, A.]]
Line 38:		Line 47:
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar 25 12~~:11:44 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 15 09:24:11 2009''