2we2 - Revision history

OCA at 15:53, 13 December 2023

OCA — Wed, 13 Dec 2023 15:53:26 GMT

←Older revision		Revision as of 15:53, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2we2' size='340' side='right'caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2we2' size='340' side='right'caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ebvb9 Ebvb9~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_herpesvirus_4_strain_B95-8 Human herpesvirus 4 strain B95-8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~, ~~<scene name='pdbligand=UMP:2-DEOXYURIDINE+~~5~~-MONOPHOSPHATE'>UMP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [https://pdbe.org/2we2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [https://www.ebi.ac.uk/pdbsum/2we2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2we2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [https://pdbe.org/2we2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [https://www.ebi.ac.uk/pdbsum/2we2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2we2 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DUT_EBVB9 DUT_EBVB9] Involved in nucleotide metabolism: produces dUMP, the immediate precursor of thymidine nucleotides and decreases the intracellular concentration of dUTP to avoid uracil incorporation into DNA. Induces immune dysregulation that contributes to the pathophysiology of the virus infection.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ebvb9~~]]	+	[[Category: Human herpesvirus 4 strain B95-8]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: DUTP diphosphatase]]~~	+	[[Category: Buisson M]]
-	[[Category: Buisson, M]]	+	[[Category: Burmeister WP]]
-	[[Category: Burmeister~~, W P~~]]	+	[[Category: Freeman L]]
-	[[Category: Freeman, L]]	+	[[Category: Tarbouriech N]]
-	[[Category: Tarbouriech, N]]	+
-	~~[[Category: Dutpase]]~~	+
-	~~[[Category: Epstein-barr virus]]~~	+
-	~~[[Category: Herpes virus]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Monomer]]~~	+
-	~~[[Category: Nucleotide metabolism~~]]	+

OCA at 11:05, 6 April 2022

OCA — Wed, 06 Apr 2022 11:05:16 GMT

←Older revision		Revision as of 11:05, 6 April 2022
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	<StructureSection load='2we2' size='340' side='right'caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2we2' size='340' side='right'caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ebvb9 Ebvb9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebvb9 Ebvb9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [~~http~~://pdbe.org/2we2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2we2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2we2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [https://pdbe.org/2we2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [https://www.ebi.ac.uk/pdbsum/2we2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2we2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:37, 1 July 2020

OCA — Wed, 01 Jul 2020 08:37:24 GMT

←Older revision		Revision as of 08:37, 1 July 2020
Line 1:		Line 1:

	==EBV dUTPase double mutant Gly78Asp-Asp131Ser with dUMP==		==EBV dUTPase double mutant Gly78Asp-Asp131Ser with dUMP==
-	<StructureSection load='2we2' size='340' side='right' caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='2we2' size='340' side='right'caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebvb9 Ebvb9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebvb9 Ebvb9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://pdbe.org/2we2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2we2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://pdbe.org/2we2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2we2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[~~Deoxyuridine 5'-triphosphate nucleotidohydrolase~~\|~~Deoxyuridine 5'-triphosphate nucleotidohydrolase~~]]	+	*[[DUTPase 3D structures\|DUTPase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Ebvb9]]		[[Category: Ebvb9]]
		+	[[Category: Large Structures]]
	[[Category: DUTP diphosphatase]]		[[Category: DUTP diphosphatase]]
	[[Category: Buisson, M]]		[[Category: Buisson, M]]

OCA at 09:02, 26 September 2018

OCA — Wed, 26 Sep 2018 09:02:48 GMT

←Older revision		Revision as of 09:02, 26 September 2018
Line 1:		Line 1:
-	==EBV ~~DUTPASE DOUBLE MUTANT GLY78ASP~~-~~ASP131SER WITH DUMP~~==	+
		+	==EBV dUTPase double mutant Gly78Asp-Asp131Ser with dUMP==
	<StructureSection load='2we2' size='340' side='right' caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2we2' size='340' side='right' caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://pdbe.org/2we2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://pdbe.org/2we2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2we2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/we/2we2_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/we/2we2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 05:46, 9 February 2016

OCA — Tue, 09 Feb 2016 05:46:21 GMT

←Older revision		Revision as of 05:46, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2we2' size='340' side='right' caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2we2' size='340' side='right' caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Human_herpesvirus_4 Human herpesvirus 4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ebvb9 Ebvb9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://pdbe.org/2we2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2we2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2we2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human herpesvirus 4~~]]	+	[[Category: Ebvb9]]
	[[Category: DUTP diphosphatase]]		[[Category: DUTP diphosphatase]]
	[[Category: Buisson, M]]		[[Category: Buisson, M]]

OCA at 07:50, 20 January 2015

OCA — Tue, 20 Jan 2015 07:50:31 GMT

←Older revision		Revision as of 07:50, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Human herpesvirus 4]]		[[Category: Human herpesvirus 4]]
	[[Category: DUTP diphosphatase]]		[[Category: DUTP diphosphatase]]
-	[[Category: Buisson, M.]]	+	[[Category: Buisson, M]]
-	[[Category: Burmeister, W P.]]	+	[[Category: Burmeister, W P]]
-	[[Category: Freeman, L.]]	+	[[Category: Freeman, L]]
-	[[Category: Tarbouriech, N.]]	+	[[Category: Tarbouriech, N]]
	[[Category: Dutpase]]		[[Category: Dutpase]]
	[[Category: Epstein-barr virus]]		[[Category: Epstein-barr virus]]

OCA at 06:26, 29 September 2014

OCA — Mon, 29 Sep 2014 06:26:19 GMT

←Older revision		Revision as of 06:26, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2we2.~~png~~\|~~left~~\|200px]]	+	==EBV DUTPASE DOUBLE MUTANT GLY78ASP-ASP131SER WITH DUMP==
		+	<StructureSection load='2we2' size='340' side='right' caption='[[2we2]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WE2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UMP:2-DEOXYURIDINE+5-MONOPHOSPHATE'>UMP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2bsy\|2bsy]], [[2bt1\|2bt1]], [[2we1\|2we1]], [[2we3\|2we3]], [[2we0\|2we0]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2we2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2we2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2we2 RCSB], [http://www.ebi.ac.uk/pdbsum/2we2 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/we/2we2_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Deoxyuridine 5'-triphosphate pyrophosphatases (dUTPases) are ubiquitous enzymes essential for hydrolysis of dUTP, thus preventing its incorporation into DNA. Although Epstein-Barr virus (EBV) dUTPase is monomeric, it has a high degree of similarity with the more frequent trimeric form of the enzyme. In both cases, the active site is composed of five conserved sequence motifs. Structural and functional studies of mutants based on the structure of EBV dUTPase gave new insight into the mechanism of the enzyme. A first mutant allowed us to exclude a role in enzymatic activity for the disulfide bridge involving the beginning of the disordered C terminus. Sequence alignments revealed two groups of dUTPases, based on the position in sequence of a conserved aspartic acid residue close to the active site. Single mutants of this residue in EBV dUTPase showed a highly impaired catalytic activity, which could be partially restored by a second mutation, making EBV dUTPase more similar to the second group of enzymes. Deletion of the flexible C-terminal tail carrying motif V resulted in a protein completely devoid of enzymatic activity, crystallizing with unhydrolyzed Mg(2+)-dUTP complex in the active site. Point mutations inside motif V highlighted the essential role of lid residue Phe(273). Magnesium appears to play a role mainly in substrate binding, since in absence of Mg(2+), the K(m) of the enzyme is reduced, whereas the k(cat) is less affected.

-	~~{{STRUCTURE_2we2\| PDB=2we2 \| SCENE= }}~~	+	The flexible motif V of Epstein-Barr virus deoxyuridine 5'-triphosphate pyrophosphatase is essential for catalysis.,Freeman L, Buisson M, Tarbouriech N, Van der Heyden A, Labbe P, Burmeister WP J Biol Chem. 2009 Sep 11;284(37):25280-9. Epub 2009 Jul 7. PMID:19586911<ref>PMID:19586911</ref>

-	~~===EBV DUTPASE DOUBLE MUTANT GLY78ASP-ASP131SER WITH DUMP===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19586911}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2we2]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Human_herpesvirus_4 Human herpesvirus 4]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA].~~	+

	==See Also==		==See Also==
	*[[Deoxyuridine 5'-triphosphate nucleotidohydrolase\|Deoxyuridine 5'-triphosphate nucleotidohydrolase]]		*[[Deoxyuridine 5'-triphosphate nucleotidohydrolase\|Deoxyuridine 5'-triphosphate nucleotidohydrolase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019586911<~~/~~ref~~><~~ref group="xtra">PMID:016154087</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Human herpesvirus 4]]		[[Category: Human herpesvirus 4]]
	[[Category: DUTP diphosphatase]]		[[Category: DUTP diphosphatase]]

OCA at 12:50, 20 October 2012

OCA — Sat, 20 Oct 2012 12:50:50 GMT

←Older revision		Revision as of 12:50, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2we2]] is a 1 chain structure ~~of [[Deoxyuridine 5'-triphosphate nucleotidohydrolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA].	+	[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA].

	==See Also==		==See Also==

OCA at 10:04, 26 July 2012

OCA — Thu, 26 Jul 2012 10:04:46 GMT

←Older revision		Revision as of 10:04, 26 July 2012
Line 1:		Line 1:
	[[Image:2we2.png\|left\|200px]]		[[Image:2we2.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2we2", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2we2\| PDB=2we2 \| SCENE= }}		{{STRUCTURE_2we2\| PDB=2we2 \| SCENE= }}

	===EBV DUTPASE DOUBLE MUTANT GLY78ASP-ASP131SER WITH DUMP===		===EBV DUTPASE DOUBLE MUTANT GLY78ASP-ASP131SER WITH DUMP===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19586911}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19586911 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19586911}}		{{ABSTRACT_PUBMED_19586911}}

	==About this Structure==		==About this Structure==
-	[[2we2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA].	+	[[2we2]] is a 1 chain structure of [[Deoxyuridine 5'-triphosphate nucleotidohydrolase]] with sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WE2 OCA].
		+
		+	==See Also==
		+	*[[Deoxyuridine 5'-triphosphate nucleotidohydrolase\|Deoxyuridine 5'-triphosphate nucleotidohydrolase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19586911~~</ref><ref group="xtra">PMID:~~16154087~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019586911</ref><ref group="xtra">PMID:016154087</ref><references group="xtra"/>
	[[Category: Human herpesvirus 4]]		[[Category: Human herpesvirus 4]]
	[[Category: DUTP diphosphatase]]		[[Category: DUTP diphosphatase]]

OCA: Protected "2we2" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 18:05:25 GMT

Protected "2we2" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:05, 14 March 2011