2wgs - Revision history

OCA at 15:55, 13 December 2023

OCA — Wed, 13 Dec 2023 15:55:35 GMT

←Older revision		Revision as of 15:55, 13 December 2023
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	<StructureSection load='2wgs' size='340' side='right'caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='2wgs' size='340' side='right'caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WGS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WGS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [https://pdbe.org/2wgs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [https://www.ebi.ac.uk/pdbsum/2wgs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [https://pdbe.org/2wgs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [https://www.ebi.ac.uk/pdbsum/2wgs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GLN1B_MYCTU GLN1B_MYCTU] Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia (PubMed:7937767, PubMed:12819079). Also able to use GTP (PubMed:7937767). D-glutamate is a poor substrate, and DL-glutamate shows about 50% of the standard specific activity (PubMed:7937767). Also plays a key role in controlling the ammonia levels within infected host cells and so contributes to the pathogens capacity to inhibit phagosome acidification and phagosome-lysosome fusion (PubMed:7937767, PubMed:12819079). Involved in cell wall biosynthesis via the production of the major component poly-L-glutamine (PLG) (PubMed:7937767, PubMed:10618433). PLG synthesis in the cell wall occurs only in nitrogen limiting conditions and on the contrary high nitrogen conditions inhibit PLG synthesis (Probable).<ref>PMID:10618433</ref> <ref>PMID:12819079</ref> <ref>PMID:7937767</ref> <ref>PMID:9278431</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Glutamate--ammonia ligase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Jones~~, T A~~]]	+	[[Category: Jones TA]]
-	[[Category: Krajewski~~, W W~~]]	+	[[Category: Krajewski WW]]
-	[[Category: Mowbray~~, S L~~]]	+	[[Category: Mowbray SL]]
-	[[Category: Nilsson~~, M T]]~~	+	[[Category: Nilsson MT]]
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Glna1]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Mt2278]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Purine analogue]]~~	+
-	~~[[Category: Relaxed state]]~~	+
-	~~[[Category: Rv2220]]~~	+
-	~~[[Category: Synthetase~~]]	+

OCA at 10:54, 7 July 2021

OCA — Wed, 07 Jul 2021 10:54:45 GMT

←Older revision		Revision as of 10:54, 7 July 2021
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	<StructureSection load='2wgs' size='340' side='right'caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='2wgs' size='340' side='right'caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WGS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WGS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [~~http~~://pdbe.org/2wgs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wgs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [https://pdbe.org/2wgs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [https://www.ebi.ac.uk/pdbsum/2wgs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgs ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[Glutamine synthetase\|Glutamine synthetase]]	+	*[[Glutamine synthetase 3D structures\|Glutamine synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:18, 10 May 2019

OCA — Fri, 10 May 2019 12:18:29 GMT

←Older revision		Revision as of 12:18, 10 May 2019
Line 1:		Line 1:

	==Crystal structure of Mycobacterium Tuberculosis Glutamine Synthetase in complex with a purine analogue inhibitor.==		==Crystal structure of Mycobacterium Tuberculosis Glutamine Synthetase in complex with a purine analogue inhibitor.==
-	<StructureSection load='2wgs' size='340' side='right' caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='2wgs' size='340' side='right'caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WGS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WGS FirstGlance]. <br>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
		+	[[Category: Large Structures]]
	[[Category: Myctu]]		[[Category: Myctu]]
	[[Category: Jones, T A]]		[[Category: Jones, T A]]

OCA at 09:16, 26 September 2018

OCA — Wed, 26 Sep 2018 09:16:54 GMT

←Older revision		Revision as of 09:16, 26 September 2018
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH A PURINE ANALOGUE INHIBITOR~~.==	+
		+	==Crystal structure of Mycobacterium Tuberculosis Glutamine Synthetase in complex with a purine analogue inhibitor.==
	<StructureSection load='2wgs' size='340' side='right' caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='2wgs' size='340' side='right' caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [http://pdbe.org/2wgs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [http://www.ebi.ac.uk/pdbsum/2wgs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [http://pdbe.org/2wgs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [http://www.ebi.ac.uk/pdbsum/2wgs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wgs ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wgs_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wgs_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	==See Also==		==See Also==
-	*[[Glutamine ~~Synthase~~\|Glutamine ~~Synthase~~]]	+	*[[Glutamine synthetase\|Glutamine synthetase]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Mowbray, S L]]		[[Category: Mowbray, S L]]
	[[Category: Nilsson, M T]]		[[Category: Nilsson, M T]]
		+	[[Category: Cytoplasm]]
	[[Category: Glna1]]		[[Category: Glna1]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 15:03, 9 February 2016

OCA — Tue, 09 Feb 2016 15:03:03 GMT

←Older revision		Revision as of 15:03, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2wgs' size='340' side='right' caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='2wgs' size='340' side='right' caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium tuberculosis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WGS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WGS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [http://www.ebi.ac.uk/pdbsum/2wgs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [http://pdbe.org/2wgs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [http://www.ebi.ac.uk/pdbsum/2wgs PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wgs ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wgs" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
-	[[Category: ~~Mycobacterium tuberculosis~~]]	+	[[Category: Myctu]]
	[[Category: Jones, T A]]		[[Category: Jones, T A]]
	[[Category: Krajewski, W W]]		[[Category: Krajewski, W W]]

OCA at 09:20, 20 January 2015

OCA — Tue, 20 Jan 2015 09:20:28 GMT

←Older revision		Revision as of 09:20, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WGS FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WGS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [http://www.ebi.ac.uk/pdbsum/2wgs PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [http://www.ebi.ac.uk/pdbsum/2wgs PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
-	[[Category: Jones, T A.]]	+	[[Category: Jones, T A]]
-	[[Category: Krajewski, W W.]]	+	[[Category: Krajewski, W W]]
-	[[Category: Mowbray, S L.]]	+	[[Category: Mowbray, S L]]
-	[[Category: Nilsson, M T.]]	+	[[Category: Nilsson, M T]]
	[[Category: Glna1]]		[[Category: Glna1]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA at 07:45, 29 September 2014

OCA — Mon, 29 Sep 2014 07:45:22 GMT

←Older revision		Revision as of 07:45, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2wgs.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH A PURINE ANALOGUE INHIBITOR.==
		+	<StructureSection load='2wgs' size='340' side='right' caption='[[2wgs]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WGS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1AZ:1-(3,4-DICHLOROBENZYL)-3,7-DIMETHYL-8-MORPHOLIN-4-YL-3,7-DIHYDRO-1H-PURINE-2,6-DIONE'>1AZ</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hto\|1hto]], [[1htq\|1htq]], [[2whi\|2whi]], [[2bvc\|2bvc]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wgs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wgs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wgs RCSB], [http://www.ebi.ac.uk/pdbsum/2wgs PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wg/2wgs_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutamine synthetase (GS, EC 6.3.1.2; also known as gamma-glutamyl:ammonia ligase) catalyzes the ATP-dependent condensation of glutamate and ammonia to form glutamine. The enzyme has essential roles in different tissues and species, which have led to its consideration as a drug or an herbicide target. In this article, we describe studies aimed at the discovery of new antimicrobial agents targeting Mycobacterium tuberculosis, the causative pathogen of tuberculosis. A number of distinct classes of GS inhibitors with an IC(50) of micromolar value or better were identified via high-throughput screening. A commercially available purine analogue similar to one of the clusters identified (the diketopurines), 1-[(3,4-dichlorophenyl)methyl]-3,7-dimethyl-8-morpholin-4-yl-purine-2,6-di one, was also shown to inhibit the enzyme, with a measured IC(50) of 2.5+/-0.4 microM. Two X-ray structures are presented: one is a complex of the enzyme with the purine analogue alone (2.55-A resolution), and the other includes the compound together with methionine sulfoximine phosphate, magnesium and phosphate (2.2-A resolution). The former represents a relaxed, inactive conformation of the enzyme, while the latter is a taut, active one. These structures show that the compound binds at the same position in the nucleotide site, regardless of the conformational state. The ATP-binding site of the human enzyme differs substantially, explaining why it has an approximately 60-fold lower affinity for this compound than the bacterial GS. As part of this work, we devised a new synthetic procedure for generating l-(SR)-methionine sulfoximine phosphate from l-(SR)-methionine sulfoximine, which will facilitate future investigations of novel GS inhibitors.

-	~~{{STRUCTURE_2wgs\| PDB=2wgs \| SCENE= }}~~	+	Structural basis for the inhibition of Mycobacterium tuberculosis glutamine synthetase by novel ATP-competitive inhibitors.,Nilsson MT, Krajewski WW, Yellagunda S, Prabhumurthy S, Chamarahally GN, Siddamadappa C, Srinivasa BR, Yahiaoui S, Larhed M, Karlen A, Jones TA, Mowbray SL J Mol Biol. 2009 Oct 23;393(2):504-13. Epub 2009 Aug 18. PMID:19695264<ref>PMID:19695264</ref>

-	~~===CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH A PURINE ANALOGUE INHIBITOR.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19695264}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2wgs]] is~~ a ~~12 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA].~~	+

	==See Also==		==See Also==
	*[[Glutamine Synthase\|Glutamine Synthase]]		*[[Glutamine Synthase\|Glutamine Synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019695264<~~/~~ref~~><~~ref group="xtra">PMID:016027359</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]

OCA at 14:14, 20 October 2012

OCA — Sat, 20 Oct 2012 14:14:32 GMT

←Older revision		Revision as of 14:14, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2wgs]] is a 12 chain structure ~~of [[Glutamine Synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA].	+	[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA].

	==See Also==		==See Also==

OCA at 14:31, 26 July 2012

OCA — Thu, 26 Jul 2012 14:31:22 GMT

←Older revision		Revision as of 14:31, 26 July 2012
Line 1:		Line 1:
	[[Image:2wgs.png\|left\|200px]]		[[Image:2wgs.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_2wgs\| PDB=2wgs \| SCENE= }}		{{STRUCTURE_2wgs\| PDB=2wgs \| SCENE= }}

	===CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH A PURINE ANALOGUE INHIBITOR.===		===CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH A PURINE ANALOGUE INHIBITOR.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19695264}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19695264 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19695264}}		{{ABSTRACT_PUBMED_19695264}}

	==About this Structure==		==About this Structure==
-	[[2wgs]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA].	+	[[2wgs]] is a 12 chain structure of [[Glutamine Synthase]] with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WGS OCA].
		+
		+	==See Also==
		+	*[[Glutamine Synthase\|Glutamine Synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19695264~~</ref><ref group="xtra">PMID:~~16027359~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019695264</ref><ref group="xtra">PMID:016027359</ref><references group="xtra"/>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
Line 29:		Line 21:
	[[Category: Mowbray, S L.]]		[[Category: Mowbray, S L.]]
	[[Category: Nilsson, M T.]]		[[Category: Nilsson, M T.]]
-	[[Category: Cytoplasm]]
	[[Category: Glna1]]		[[Category: Glna1]]
	[[Category: Ligase]]		[[Category: Ligase]]

OCA: Protected "2wgs" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 20:18:06 GMT

Protected "2wgs" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:18, 14 March 2011