2wio - Revision history

OCA at 15:58, 13 December 2023

OCA — Wed, 13 Dec 2023 15:58:45 GMT

←Older revision		Revision as of 15:58, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2wio' size='340' side='right'caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wio' size='340' side='right'caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WIO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WIO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [https://pdbe.org/2wio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [https://www.ebi.ac.uk/pdbsum/2wio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wio ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [https://pdbe.org/2wio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [https://www.ebi.ac.uk/pdbsum/2wio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wio ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ERYK_SACEN ERYK_SACEN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gianni, S]]	+	[[Category: Saccharopolyspora erythraea]]
-	[[Category: Kedrew~~, S G~~]]	+	[[Category: Gianni S]]
-	[[Category: Miele~~, A E~~]]	+	[[Category: Kedrew SG]]
-	[[Category: Montemiglio~~, L C~~]]	+	[[Category: Miele AE]]
-	[[Category: Savino, C]]	+	[[Category: Montemiglio LC]]
-	[[Category: Sciara, G]]	+	[[Category: Savino C]]
-	[[Category: Vallone, B]]	+	[[Category: Sciara G]]
-	~~[[Category: Antibiotic biosynthesis]]~~	+	[[Category: Vallone B]]
-	~~[[Category: Cytochrome p450]]~~	+
-	~~[[Category: Erythromicyn]]~~	+
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Iron]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Substrate specificity~~]]	+

OCA at 11:08, 6 April 2022

OCA — Wed, 06 Apr 2022 11:08:28 GMT

←Older revision		Revision as of 11:08, 6 April 2022
Line 3:		Line 3:
	<StructureSection load='2wio' size='340' side='right'caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wio' size='340' side='right'caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WIO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WIO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [~~http~~://pdbe.org/2wio PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wio PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wio ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [https://pdbe.org/2wio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [https://www.ebi.ac.uk/pdbsum/2wio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wio ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:47, 1 July 2020

OCA — Wed, 01 Jul 2020 08:47:34 GMT

←Older revision		Revision as of 08:47, 1 July 2020
Line 1:		Line 1:

	==Structure of the histidine tagged, open cytochrome P450 Eryk from S. erythraea==		==Structure of the histidine tagged, open cytochrome P450 Eryk from S. erythraea==
-	<StructureSection load='2wio' size='340' side='right' caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='2wio' size='340' side='right'caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WIO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WIO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://pdbe.org/2wio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wio ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://pdbe.org/2wio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wio ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gianni, S]]		[[Category: Gianni, S]]
	[[Category: Kedrew, S G]]		[[Category: Kedrew, S G]]

OCA at 08:52, 26 September 2018

OCA — Wed, 26 Sep 2018 08:52:17 GMT

←Older revision		Revision as of 08:52, 26 September 2018
Line 1:		Line 1:
-	==~~STRUCTURE OF THE HISTIDINE TAGGED~~, ~~OPEN CYTOCHROME~~ P450 ~~ERYK FROM~~ S. ~~ERYTHRAEA~~==	+
		+	==Structure of the histidine tagged, open cytochrome P450 Eryk from S. erythraea==
	<StructureSection load='2wio' size='340' side='right' caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wio' size='340' side='right' caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://pdbe.org/2wio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://pdbe.org/2wio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wio ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wi/2wio_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wi/2wio_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:36, 8 February 2016

OCA — Mon, 08 Feb 2016 10:36:26 GMT

←Older revision		Revision as of 10:36, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='2wio' size='340' side='right' caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='2wio' size='340' side='right' caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharopolyspora_erythraea Saccharopolyspora erythraea~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WIO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_erythreus"_(sic)_waksman_1923 "actinomyces erythreus" (sic) waksman 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WIO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://pdbe.org/2wio PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wio ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wio" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Saccharopolyspora erythraea]]
	[[Category: Gianni, S]]		[[Category: Gianni, S]]
	[[Category: Kedrew, S G]]		[[Category: Kedrew, S G]]

OCA at 08:51, 20 January 2015

OCA — Tue, 20 Jan 2015 08:51:25 GMT

←Older revision		Revision as of 08:51, 20 January 2015
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Saccharopolyspora erythraea]]		[[Category: Saccharopolyspora erythraea]]
-	[[Category: Gianni, S.]]	+	[[Category: Gianni, S]]
-	[[Category: Kedrew, S G.]]	+	[[Category: Kedrew, S G]]
-	[[Category: Miele, A E.]]	+	[[Category: Miele, A E]]
-	[[Category: Montemiglio, L C.]]	+	[[Category: Montemiglio, L C]]
-	[[Category: Savino, C.]]	+	[[Category: Savino, C]]
-	[[Category: Sciara, G.]]	+	[[Category: Sciara, G]]
-	[[Category: Vallone, B.]]	+	[[Category: Vallone, B]]
	[[Category: Antibiotic biosynthesis]]		[[Category: Antibiotic biosynthesis]]
	[[Category: Cytochrome p450]]		[[Category: Cytochrome p450]]

OCA at 06:22, 10 October 2014

OCA — Fri, 10 Oct 2014 06:22:13 GMT

←Older revision		Revision as of 06:22, 10 October 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WIO FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WIO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 23:		Line 23:
	Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.,Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B J Biol Chem. 2009 Oct 16;284(42):29170-9. Epub 2009 Jul 22. PMID:19625248<ref>PMID:19625248</ref>		Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.,Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B J Biol Chem. 2009 Oct 16;284(42):29170-9. Epub 2009 Jul 22. PMID:19625248<ref>PMID:19625248</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA: Protected "2wio" [edit=sysop:move=sysop]

OCA — Wed, 28 May 2014 10:23:19 GMT

Protected "2wio" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:23, 28 May 2014

OCA at 10:23, 28 May 2014

OCA — Wed, 28 May 2014 10:23:15 GMT

←Older revision		Revision as of 10:23, 28 May 2014
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-	~~{{Seed}}~~	+	==STRUCTURE OF THE HISTIDINE TAGGED, OPEN CYTOCHROME P450 ERYK FROM S. ERYTHRAEA==
-	[[~~Image:~~2wio.~~png~~\|~~left~~\|200px]]	+	<StructureSection load='2wio' size='340' side='right' caption='[[2wio]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WIO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vru\|2vru]], [[2jjn\|2jjn]], [[2vrv\|2vrv]], [[2jjp\|2jjp]], [[2jjo\|2jjo]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wio OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wio RCSB], [http://www.ebi.ac.uk/pdbsum/2wio PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wi/2wio_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytochrome P450s are heme-containing proteins that catalyze the oxidative metabolism of many physiological endogenous compounds. Because of their unique oxygen chemistry and their key role in drug and xenobiotic metabolism, particular attention has been devoted in elucidating their mechanism of substrate recognition. In this work, we analyzed the three-dimensional structures of a monomeric cytochrome P450 from Saccharopolyspora erythraea, commonly called EryK, and the binding kinetics to its physiological ligand, erythromycin D. Three different structures of EryK were obtained: two ligand-free forms and one in complex with its substrate. Analysis of the substrate-bound structure revealed the key structural determinants involved in substrate recognition and selectivity. Interestingly, the ligand-free structures of EryK suggested that the protein may explore an open and a closed conformation in the absence of substrate. In an effort to validate this hypothesis and to investigate the energetics between such alternative conformations, we performed stopped-flow absorbance experiments. Data demonstrated that EryK binds erythromycin D via a mechanism involving at least two steps. Contrary to previously characterized cytochrome P450s, analysis of double jump mixing experiments confirmed that this complex scenario arises from a pre-existing equilibrium between the open and closed subpopulations of EryK, rather than from an induced-fit type mechanism.

-	<!--	+	Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.,Savino C, Montemiglio LC, Sciara G, Miele AE, Kendrew SG, Jemth P, Gianni S, Vallone B J Biol Chem. 2009 Oct 16;284(42):29170-9. Epub 2009 Jul 22. PMID:19625248<ref>PMID:19625248</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2wio"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2wio\| PDB=2wio \| SCENE= }}~~	+

-	~~===STRUCTURE OF THE HISTIDINE TAGGED~~, ~~OPEN CYTOCHROME P450 ERYK FROM S. ERYTHRAEA===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_19625248}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 19625248 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_19625248}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~2WIO is a 1 chain structure of sequence from [http:~~/~~/en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:19625248<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharopolyspora erythraea]]		[[Category: Saccharopolyspora erythraea]]
	[[Category: Gianni, S.]]		[[Category: Gianni, S.]]
Line 41:		Line 46:
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Substrate specificity]]		[[Category: Substrate specificity]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 5 08:55:10 2009''

OCA at 05:55, 5 August 2009

OCA — Wed, 05 Aug 2009 05:55:11 GMT

←Older revision		Revision as of 05:55, 5 August 2009
Line 12:		Line 12:
	===STRUCTURE OF THE HISTIDINE TAGGED, OPEN CYTOCHROME P450 ERYK FROM S. ERYTHRAEA===		===STRUCTURE OF THE HISTIDINE TAGGED, OPEN CYTOCHROME P450 ERYK FROM S. ERYTHRAEA===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19625248}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19625248 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19625248}}

	==About this Structure==		==About this Structure==
	2WIO is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA].		2WIO is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharopolyspora_erythraea Saccharopolyspora erythraea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WIO OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19625248</ref><references group="xtra"/>
	[[Category: Saccharopolyspora erythraea]]		[[Category: Saccharopolyspora erythraea]]
	[[Category: Gianni, S.]]		[[Category: Gianni, S.]]
Line 33:		Line 42:
	[[Category: Substrate specificity]]		[[Category: Substrate specificity]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Jul 29 15~~:56:23 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 5 08:55:10 2009''