2wjf - Revision history

OCA at 07:10, 1 May 2024

OCA — Wed, 01 May 2024 07:10:11 GMT

←Older revision		Revision as of 07:10, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='2wjf' size='340' side='right'caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>		<StructureSection load='2wjf' size='340' side='right'caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus~~ pneumoniae~~" (klein 1884) weichselbaum 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WJF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.22Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2wjd\|2wjd]], [[2wje\|2wje]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [https://pdbe.org/2wjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [https://www.ebi.ac.uk/pdbsum/2wjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [https://pdbe.org/2wjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [https://www.ebi.ac.uk/pdbsum/2wjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjf ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CPSB_STRPN CPSB_STRPN] Dephosphorylates CpsD. Involved in the regulation of capsular polysaccharide biosynthesis (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wjf ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wjf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Many Gram-positive and Gram-negative bacteria utilize polysaccharide surface layers called capsules to evade the immune system; consequently, the synthesis and export of the capsule are a potential therapeutic target. In Escherichia coli K-30, the integral membrane tyrosine autokinase Wzc and the cognate phosphatase Wzb have been shown to be key for both synthesis and assembly of capsular polysaccharides. In the Gram-positive bacterium Streptococcus pneumoniae, the CpsCD complex is analogous to Wzc and the phosphatase CpsB is the corresponding cognate phosphatase. The phosphatases are known to dephosphorylate their corresponding autokinases, yet despite their functional equivalence, they share no sequence homology. We present the structure of Wzb in complex with phosphate and high-resolution structures of apo-CpsB and a phosphate-complexed CpsB. We show that both proteins are active toward Wzc and thereby demonstrate that CpsB is not specific for CpsCD. CpsB is a novel enzyme and represents the first solved structure of a tyrosine phosphatase from a Gram-positive bacterium. Wzb and CpsB have completely different structures, suggesting that they must operate by very different mechanisms. Although the mechanism of Wzb can be inferred from previous studies, CpsB appears to have a tyrosine phosphatase mechanism not observed before. We propose a chemical mechanism for CpsB based on site-directed mutagenesis and structural data.
-
-	Crystal structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, representatives of two families of tyrosine phosphatases that regulate capsule assembly.,Hagelueken G, Huang H, Mainprize IL, Whitfield C, Naismith JH J Mol Biol. 2009 Sep 25;392(3):678-88. Epub 2009 Jul 16. PMID:19616007<ref>PMID:19616007</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2wjf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Tyrosine phosphatase 3D structures\|Tyrosine phosphatase 3D structures]]		*[[Tyrosine phosphatase 3D structures\|Tyrosine phosphatase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Protein-tyrosine-phosphatase~~]]	+	[[Category: Streptococcus pneumoniae]]
-	[[Category: Hagelueken, G]]	+	[[Category: Hagelueken G]]
-	[[Category: Huang, H]]	+	[[Category: Huang H]]
-	[[Category: Naismith~~, J H]]~~	+	[[Category: Naismith JH]]
-	~~[[Category: Capsule biogenesis/degradation]]~~	+
-	~~[[Category: Exopolysaccharide synthesis]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Manganese]]~~	+
-	~~[[Category: Phosphatase]]~~	+
-	~~[[Category: Protein phosphatase~~]]	+

OCA at 11:08, 6 April 2022

OCA — Wed, 06 Apr 2022 11:08:55 GMT

←Older revision		Revision as of 11:08, 6 April 2022
Line 3:		Line 3:
	<StructureSection load='2wjf' size='340' side='right'caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>		<StructureSection load='2wjf' size='340' side='right'caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WJF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wjd\|2wjd]], [[2wje\|2wje]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [~~http~~://pdbe.org/2wjf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wjf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [https://pdbe.org/2wjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [https://www.ebi.ac.uk/pdbsum/2wjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjf ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 08:48, 1 July 2020

OCA — Wed, 01 Jul 2020 08:48:43 GMT

←Older revision		Revision as of 08:48, 1 July 2020
Line 1:		Line 1:

	==Crystal structure of the tyrosine phosphatase Cps4B from Steptococcus pneumoniae TIGR4 in complex with phosphate.==		==Crystal structure of the tyrosine phosphatase Cps4B from Steptococcus pneumoniae TIGR4 in complex with phosphate.==
-	<StructureSection load='2wjf' size='340' side='right' caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>	+	<StructureSection load='2wjf' size='340' side='right'caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WJF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://pdbe.org/2wjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://pdbe.org/2wjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjf ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Tyrosine phosphatase\|Tyrosine phosphatase]]	+	*[[Tyrosine phosphatase 3D structures\|Tyrosine phosphatase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Protein-tyrosine-phosphatase]]		[[Category: Protein-tyrosine-phosphatase]]
	[[Category: Hagelueken, G]]		[[Category: Hagelueken, G]]

OCA at 09:17, 26 September 2018

OCA — Wed, 26 Sep 2018 09:17:27 GMT

←Older revision		Revision as of 09:17, 26 September 2018
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF THE TYROSINE PHOSPHATASE CPS4B FROM STEPTOCOCCUS PNEUMONIAE~~ TIGR4 ~~IN COMPLEX WITH PHOSPHATE~~.==	+
		+	==Crystal structure of the tyrosine phosphatase Cps4B from Steptococcus pneumoniae TIGR4 in complex with phosphate.==
	<StructureSection load='2wjf' size='340' side='right' caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>		<StructureSection load='2wjf' size='340' side='right' caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://pdbe.org/2wjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://pdbe.org/2wjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjf ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wj/2wjf_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wj/2wjf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:04, 9 February 2016

OCA — Tue, 09 Feb 2016 15:04:00 GMT

←Older revision		Revision as of 15:04, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2wjf' size='340' side='right' caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>		<StructureSection load='2wjf' size='340' side='right' caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_pneumoniae Streptococcus~~ pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://pdbe.org/2wjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wjf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wjf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Protein-tyrosine-phosphatase]]		[[Category: Protein-tyrosine-phosphatase]]
-	[[Category: Streptococcus pneumoniae]]
	[[Category: Hagelueken, G]]		[[Category: Hagelueken, G]]
	[[Category: Huang, H]]		[[Category: Huang, H]]

OCA at 09:21, 20 January 2015

OCA — Tue, 20 Jan 2015 09:21:10 GMT

←Older revision		Revision as of 09:21, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJF FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Protein-tyrosine-phosphatase]]		[[Category: Protein-tyrosine-phosphatase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
-	[[Category: Hagelueken, G.]]	+	[[Category: Hagelueken, G]]
-	[[Category: Huang, H.]]	+	[[Category: Huang, H]]
-	[[Category: Naismith, J H.]]	+	[[Category: Naismith, J H]]
	[[Category: Capsule biogenesis/degradation]]		[[Category: Capsule biogenesis/degradation]]
	[[Category: Exopolysaccharide synthesis]]		[[Category: Exopolysaccharide synthesis]]

OCA at 07:45, 29 September 2014

OCA — Mon, 29 Sep 2014 07:45:41 GMT

←Older revision		Revision as of 07:45, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2wjf.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE TYROSINE PHOSPHATASE CPS4B FROM STEPTOCOCCUS PNEUMONIAE TIGR4 IN COMPLEX WITH PHOSPHATE.==
		+	<StructureSection load='2wjf' size='340' side='right' caption='[[2wjf]], [[Resolution\|resolution]] 2.22Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wjd\|2wjd]], [[2wje\|2wje]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wjf RCSB], [http://www.ebi.ac.uk/pdbsum/2wjf PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wj/2wjf_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many Gram-positive and Gram-negative bacteria utilize polysaccharide surface layers called capsules to evade the immune system; consequently, the synthesis and export of the capsule are a potential therapeutic target. In Escherichia coli K-30, the integral membrane tyrosine autokinase Wzc and the cognate phosphatase Wzb have been shown to be key for both synthesis and assembly of capsular polysaccharides. In the Gram-positive bacterium Streptococcus pneumoniae, the CpsCD complex is analogous to Wzc and the phosphatase CpsB is the corresponding cognate phosphatase. The phosphatases are known to dephosphorylate their corresponding autokinases, yet despite their functional equivalence, they share no sequence homology. We present the structure of Wzb in complex with phosphate and high-resolution structures of apo-CpsB and a phosphate-complexed CpsB. We show that both proteins are active toward Wzc and thereby demonstrate that CpsB is not specific for CpsCD. CpsB is a novel enzyme and represents the first solved structure of a tyrosine phosphatase from a Gram-positive bacterium. Wzb and CpsB have completely different structures, suggesting that they must operate by very different mechanisms. Although the mechanism of Wzb can be inferred from previous studies, CpsB appears to have a tyrosine phosphatase mechanism not observed before. We propose a chemical mechanism for CpsB based on site-directed mutagenesis and structural data.

-	~~{{STRUCTURE_2wjf\| PDB=2wjf \| SCENE= }}~~	+	Crystal structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, representatives of two families of tyrosine phosphatases that regulate capsule assembly.,Hagelueken G, Huang H, Mainprize IL, Whitfield C, Naismith JH J Mol Biol. 2009 Sep 25;392(3):678-88. Epub 2009 Jul 16. PMID:19616007<ref>PMID:19616007</ref>

-	~~===CRYSTAL STRUCTURE OF THE TYROSINE PHOSPHATASE CPS4B FROM STEPTOCOCCUS PNEUMONIAE TIGR4 IN COMPLEX WITH PHOSPHATE.===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19616007}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2wjf]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA].~~	+

	==See Also==		==See Also==
	*[[Tyrosine phosphatase\|Tyrosine phosphatase]]		*[[Tyrosine phosphatase\|Tyrosine phosphatase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019616007<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Protein-tyrosine-phosphatase]]		[[Category: Protein-tyrosine-phosphatase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]

OCA at 14:14, 20 October 2012

OCA — Sat, 20 Oct 2012 14:14:47 GMT

←Older revision		Revision as of 14:14, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2wjf]] is a 1 chain structure ~~of [[Tyrosine phosphatase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA].	+	[[2wjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA].

	==See Also==		==See Also==

OCA: Protected "2wjf" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 14:32:03 GMT

Protected "2wjf" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:32, 26 July 2012

OCA at 14:32, 26 July 2012

OCA — Thu, 26 Jul 2012 14:32:00 GMT

←Older revision		Revision as of 14:32, 26 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2wjf.png\|left\|200px]]		[[Image:2wjf.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2wjf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2wjf\| PDB=2wjf \| SCENE= }}		{{STRUCTURE_2wjf\| PDB=2wjf \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE TYROSINE PHOSPHATASE CPS4B FROM STEPTOCOCCUS PNEUMONIAE TIGR4 IN COMPLEX WITH PHOSPHATE.===		===CRYSTAL STRUCTURE OF THE TYROSINE PHOSPHATASE CPS4B FROM STEPTOCOCCUS PNEUMONIAE TIGR4 IN COMPLEX WITH PHOSPHATE.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19616007}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19616007 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19616007}}		{{ABSTRACT_PUBMED_19616007}}

	==About this Structure==		==About this Structure==
-	~~2WJF~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA].	+	[[2wjf]] is a 1 chain structure of [[Tyrosine phosphatase]] with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJF OCA].
		+
		+	==See Also==
		+	*[[Tyrosine phosphatase\|Tyrosine phosphatase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19616007~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019616007</ref><references group="xtra"/>
	[[Category: Protein-tyrosine-phosphatase]]		[[Category: Protein-tyrosine-phosphatase]]
	[[Category: Streptococcus pneumoniae]]		[[Category: Streptococcus pneumoniae]]
Line 35:		Line 26:
	[[Category: Phosphatase]]		[[Category: Phosphatase]]
	[[Category: Protein phosphatase]]		[[Category: Protein phosphatase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 29 12:36:18 2009''