2wjz - Revision history

OCA at 15:59, 13 December 2023

OCA — Wed, 13 Dec 2023 15:59:33 GMT

←Older revision		Revision as of 15:59, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='2wjz' size='340' side='right'caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2wjz' size='340' side='right'caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_43589 Atcc 43589~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.601Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [https://pdbe.org/2wjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [https://www.ebi.ac.uk/pdbsum/2wjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [https://pdbe.org/2wjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [https://www.ebi.ac.uk/pdbsum/2wjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HIS6_THEMA HIS6_THEMA]] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013] [[https://www.uniprot.org/uniprot/HIS5_THEMA HIS5_THEMA]] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.[HAMAP-Rule:MF_00278]	+	[https://www.uniprot.org/uniprot/HIS6_THEMA HIS6_THEMA] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[~~Indole-3-glycerol phosphate synthase~~\|~~Indole-3-glycerol phosphate synthase~~]]	+	*[[IGPS 3D structures\|IGPS 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 43589]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Babinger, K]]	+	[[Category: Thermotoga maritima]]
-	[[Category: Haeger~~, M C~~]]	+	[[Category: Babinger K]]
-	[[Category: Kuper, J]]	+	[[Category: Haeger MC]]
-	[[Category: List, F]]	+	[[Category: Kuper J]]
-	[[Category: Razeto, A]]	+	[[Category: List F]]
-	[[Category: Sterner, R]]	+	[[Category: Razeto A]]
-	[[Category: Vega~~, M C~~]]	+	[[Category: Sterner R]]
-	[[Category: Wilmanns, M]]	+	[[Category: Vega MC]]
-	~~[[Category: Amino-acid biosynthesis]]~~	+	[[Category: Wilmanns M]]
-	~~[[Category: Ammonia channel]]~~	+
-	~~[[Category: Cyclase]]~~	+
-	~~[[Category: Glutaminase]]~~	+
-	~~[[Category: Histidine biosynthesis]]~~	+
-	~~[[Category: Lyase-transferase complex~~]]	+

OCA at 11:09, 6 April 2022

OCA — Wed, 06 Apr 2022 11:09:20 GMT

←Older revision		Revision as of 11:09, 6 April 2022
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	<StructureSection load='2wjz' size='340' side='right'caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2wjz' size='340' side='right'caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [~~http~~://pdbe.org/2wjz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wjz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [https://pdbe.org/2wjz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [https://www.ebi.ac.uk/pdbsum/2wjz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HIS6_THEMA HIS6_THEMA]] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013] [[~~http~~://www.uniprot.org/uniprot/HIS5_THEMA HIS5_THEMA]] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.[HAMAP-Rule:MF_00278]	+	[[https://www.uniprot.org/uniprot/HIS6_THEMA HIS6_THEMA]] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013] [[https://www.uniprot.org/uniprot/HIS5_THEMA HIS5_THEMA]] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.[HAMAP-Rule:MF_00278]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</div>		</div>
	<div class="pdbe-citations 2wjz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2wjz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Indole-3-glycerol phosphate synthase\|Indole-3-glycerol phosphate synthase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:50, 1 July 2020

OCA — Wed, 01 Jul 2020 08:50:16 GMT

←Older revision		Revision as of 08:50, 1 July 2020
Line 1:		Line 1:

	==Crystal structure of (HisH) K181A Y138A mutant of imidazoleglycerolphosphate synthase (HisH HisF) which displays constitutive glutaminase activity==		==Crystal structure of (HisH) K181A Y138A mutant of imidazoleglycerolphosphate synthase (HisH HisF) which displays constitutive glutaminase activity==
-	<StructureSection load='2wjz' size='340' side='right' caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='2wjz' size='340' side='right'caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [http://pdbe.org/2wjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [http://www.ebi.ac.uk/pdbsum/2wjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [http://pdbe.org/2wjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [http://www.ebi.ac.uk/pdbsum/2wjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 43589]]		[[Category: Atcc 43589]]
		+	[[Category: Large Structures]]
	[[Category: Babinger, K]]		[[Category: Babinger, K]]
	[[Category: Haeger, M C]]		[[Category: Haeger, M C]]

OCA at 09:20, 26 September 2018

OCA — Wed, 26 Sep 2018 09:20:49 GMT

←Older revision		Revision as of 09:20, 26 September 2018
Line 3:		Line 3:
	<StructureSection load='2wjz' size='340' side='right' caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2wjz' size='340' side='right' caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589~~] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</td></tr>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wj/2wjz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wj/2wjz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 43589]]		[[Category: Atcc 43589]]
-	[[Category: Thermotoga maritima]]
	[[Category: Babinger, K]]		[[Category: Babinger, K]]
	[[Category: Haeger, M C]]		[[Category: Haeger, M C]]

OCA at 09:02, 4 August 2016

OCA — Thu, 04 Aug 2016 09:02:05 GMT

←Older revision		Revision as of 09:02, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of (HisH) K181A Y138A mutant of imidazoleglycerolphosphate synthase (HisH HisF) which displays constitutive glutaminase activity==		==Crystal structure of (HisH) K181A Y138A mutant of imidazoleglycerolphosphate synthase (HisH HisF) which displays constitutive glutaminase activity==
	<StructureSection load='2wjz' size='340' side='right' caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2wjz' size='340' side='right' caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589] and [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [http://www.ebi.ac.uk/pdbsum/2wjz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [http://pdbe.org/2wjz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [http://www.ebi.ac.uk/pdbsum/2wjz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wjz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wjz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wjz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 43589]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Babinger, K]]		[[Category: Babinger, K]]

OCA at 07:37, 25 December 2014

OCA — Thu, 25 Dec 2014 07:37:34 GMT

←Older revision		Revision as of 07:37, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [http://www.ebi.ac.uk/pdbsum/2wjz PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [http://www.ebi.ac.uk/pdbsum/2wjz PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HIS6_THEMA HIS6_THEMA]] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.[HAMAP-Rule:MF_01013] [[http://www.uniprot.org/uniprot/HIS5_THEMA HIS5_THEMA]] IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.[HAMAP-Rule:MF_00278]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
-	[[Category: Babinger, K.]]	+	[[Category: Babinger, K]]
-	[[Category: Haeger, M C.]]	+	[[Category: Haeger, M C]]
-	[[Category: Kuper, J.]]	+	[[Category: Kuper, J]]
-	[[Category: List, F.]]	+	[[Category: List, F]]
-	[[Category: Razeto, A.]]	+	[[Category: Razeto, A]]
-	[[Category: Sterner, R.]]	+	[[Category: Sterner, R]]
-	[[Category: Vega, M C.]]	+	[[Category: Vega, M C]]
-	[[Category: Wilmanns, M.]]	+	[[Category: Wilmanns, M]]
	[[Category: Amino-acid biosynthesis]]		[[Category: Amino-acid biosynthesis]]
	[[Category: Ammonia channel]]		[[Category: Ammonia channel]]

OCA at 11:35, 20 October 2014

OCA — Mon, 20 Oct 2014 11:35:06 GMT

←Older revision		Revision as of 11:35, 20 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2wjz.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of (HisH) K181A Y138A mutant of imidazoleglycerolphosphate synthase (HisH HisF) which displays constitutive glutaminase activity==
		+	<StructureSection load='2wjz' size='340' side='right' caption='[[2wjz]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WJZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2a0n\|2a0n]], [[1thf\|1thf]], [[1kxj\|1kxj]], [[1gpw\|1gpw]], [[1k9v\|1k9v]], [[1vh7\|1vh7]], [[2w6r\|2w6r]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wjz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wjz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wjz RCSB], [http://www.ebi.ac.uk/pdbsum/2wjz PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wj/2wjz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nitrogen is incorporated into various metabolites by multifunctional glutamine amidotransferases via reactive ammonia generated by glutaminase hydrolysis of glutamine. Although this process is generally tightly regulated by subsequent synthase activity, little is known about how the glutaminase is inhibited in the absence of an activating signal. Here, we use imidazoleglycerolphosphate synthase as a model to investigate the mechanism of glutaminase regulation. A structure of the bienzyme-glutamine complex reveals that the glutaminase active site is in a catalysis-competent conformation but the ammonia pathway toward the synthase active site is blocked. Mutation of two residues blocking the pathway leads to a complete uncoupling of the two reactions and to a 2800-fold amplification of glutaminase activity. Our data advance the understanding of coupling enzymatic activities in glutamine amidotransferases and raise hypotheses of the underlying molecular mechanism.

-	~~{{STRUCTURE_2wjz\| PDB=2wjz \| SCENE= }}~~	+	Catalysis uncoupling in a glutamine amidotransferase bienzyme by unblocking the glutaminase active site.,List F, Vega MC, Razeto A, Hager MC, Sterner R, Wilmanns M Chem Biol. 2012 Dec 21;19(12):1589-99. doi: 10.1016/j.chembiol.2012.10.012. PMID:23261602<ref>PMID:23261602</ref>

-	~~===Crystal structure~~ of ~~(HisH) K181A Y138A mutant~~ of ~~imidazoleglycerolphosphate synthase (HisH HisF) which displays constitutive glutaminase activity===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23261602}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[2wjz]] is a 6 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA].~~	+	</StructureSection>
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Babinger, K.]]		[[Category: Babinger, K.]]

OCA at 08:18, 9 January 2013

OCA — Wed, 09 Jan 2013 08:18:12 GMT

←Older revision		Revision as of 08:18, 9 January 2013
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	{{STRUCTURE_2wjz\| PDB=2wjz \| SCENE= }}		{{STRUCTURE_2wjz\| PDB=2wjz \| SCENE= }}

-	===~~CRYSTAL STRUCTURE OF~~ (~~HISH~~) K181A Y138A ~~MUTANT OF IMIDAZOLEGLYCEROLPHOSPHATE SYNTHASE~~ (~~HISH HISF~~) ~~WHICH DISPLAYS CONSTITUTIVE GLUTAMINASE ACTIVITY~~===	+	===Crystal structure of (HisH) K181A Y138A mutant of imidazoleglycerolphosphate synthase (HisH HisF) which displays constitutive glutaminase activity===

		+	{{ABSTRACT_PUBMED_23261602}}

	==About this Structure==		==About this Structure==

OCA: Protected "2wjz" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 18:47:41 GMT

Protected "2wjz" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:47, 7 January 2013

OCA at 18:47, 7 January 2013

OCA — Mon, 07 Jan 2013 18:47:38 GMT

←Older revision		Revision as of 18:47, 7 January 2013
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:2wjz.png\|left\|200px]]
-	[[Image:2wjz.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2wjz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2wjz\| PDB=2wjz \| SCENE= }}		{{STRUCTURE_2wjz\| PDB=2wjz \| SCENE= }}

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	==About this Structure==		==About this Structure==
-	~~2WJZ~~ is a 6 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA].	+	[[2wjz]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WJZ OCA].
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
	[[Category: Babinger, K.]]		[[Category: Babinger, K.]]
Line 30:		Line 23:
	[[Category: Histidine biosynthesis]]		[[Category: Histidine biosynthesis]]
	[[Category: Lyase-transferase complex]]		[[Category: Lyase-transferase complex]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 25 08:48:44 2010''