2wp1 - Revision history

OCA at 10:12, 20 December 2023

OCA — Wed, 20 Dec 2023 10:12:52 GMT

←Older revision		Revision as of 10:12, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2wp1' size='340' side='right'caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2wp1' size='340' side='right'caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=ALY:N(6)~~-~~ACETYLLYSINE'>ALY</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2wp2\|2wp2]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [https://pdbe.org/2wp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [https://www.ebi.ac.uk/pdbsum/2wp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wp1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [https://pdbe.org/2wp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [https://www.ebi.ac.uk/pdbsum/2wp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wp1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BRDT_MOUSE BRDT_MOUSE]] Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.<ref>PMID:12861021</ref> <ref>PMID:22901802</ref> <ref>PMID:22922464</ref> <ref>PMID:22570411</ref> <ref>PMID:19794495</ref>	+	[https://www.uniprot.org/uniprot/BRDT_MOUSE BRDT_MOUSE] Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.<ref>PMID:12861021</ref> <ref>PMID:22901802</ref> <ref>PMID:22922464</ref> <ref>PMID:22570411</ref> <ref>PMID:19794495</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Curtet, S]]	+	[[Category: Curtet S]]
-	[[Category: Gaucher, J]]	+	[[Category: Gaucher J]]
-	[[Category: Govin, J]]	+	[[Category: Govin J]]
-	[[Category: Hart~~, D J~~]]	+	[[Category: Hart DJ]]
-	[[Category: Khochbin, S]]	+	[[Category: Khochbin S]]
-	[[Category: Krijgsveld, J]]	+	[[Category: Krijgsveld J]]
-	[[Category: Moriniere, J]]	+	[[Category: Moriniere J]]
-	[[Category: Mueller~~, C W~~]]	+	[[Category: Mueller CW]]
-	[[Category: Petosa, C]]	+	[[Category: Petosa C]]
-	[[Category: Rousseaux, S]]	+	[[Category: Rousseaux S]]
-	[[Category: Sadoul, K]]	+	[[Category: Sadoul K]]
-	[[Category: Soler-Lopez, M]]	+	[[Category: Soler-Lopez M]]
-	[[Category: Steuerwald, U]]	+	[[Category: Steuerwald U]]
-	[[Category: Vitte, A L]]	+	[[Category: Vitte A-L]]
-	~~[[Category: Acetyllysine brdt]]~~	+
-	~~[[Category: Chromosomal protein]]~~	+
-	~~[[Category: Coiled coil]]~~	+
-	~~[[Category: Nucleosome]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Transcription peptide complex]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Transcription-peptide complex~~]]	+

OCA at 10:57, 7 July 2021

OCA — Wed, 07 Jul 2021 10:57:26 GMT

←Older revision		Revision as of 10:57, 7 July 2021
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	<StructureSection load='2wp1' size='340' side='right'caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2wp1' size='340' side='right'caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wp2\|2wp2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2wp2\|2wp2]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [~~http~~://pdbe.org/2wp1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2wp1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2wp1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [https://pdbe.org/2wp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [https://www.ebi.ac.uk/pdbsum/2wp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wp1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BRDT_MOUSE BRDT_MOUSE]] Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.<ref>PMID:12861021</ref> <ref>PMID:22901802</ref> <ref>PMID:22922464</ref> <ref>PMID:22570411</ref> <ref>PMID:19794495</ref>	+	[[https://www.uniprot.org/uniprot/BRDT_MOUSE BRDT_MOUSE]] Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.<ref>PMID:12861021</ref> <ref>PMID:22901802</ref> <ref>PMID:22922464</ref> <ref>PMID:22570411</ref> <ref>PMID:19794495</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:19, 10 May 2019

OCA — Fri, 10 May 2019 12:19:48 GMT

←Older revision		Revision as of 12:19, 10 May 2019
Line 1:		Line 1:

	==Structure of Brdt bromodomain 2 bound to an acetylated histone H3 peptide==		==Structure of Brdt bromodomain 2 bound to an acetylated histone H3 peptide==
-	<StructureSection load='2wp1' size='340' side='right' caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='2wp1' size='340' side='right'caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Curtet, S]]		[[Category: Curtet, S]]

OCA at 09:05, 26 September 2018

OCA — Wed, 26 Sep 2018 09:05:29 GMT

←Older revision		Revision as of 09:05, 26 September 2018
Line 1:		Line 1:
		+
	==Structure of Brdt bromodomain 2 bound to an acetylated histone H3 peptide==		==Structure of Brdt bromodomain 2 bound to an acetylated histone H3 peptide==
	<StructureSection load='2wp1' size='340' side='right' caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2wp1' size='340' side='right' caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wp2\|2wp2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wp2\|2wp2]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://pdbe.org/2wp1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://pdbe.org/2wp1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2wp1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/2wp1_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/2wp1_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	[[Category: Acetyllysine brdt]]		[[Category: Acetyllysine brdt]]
	[[Category: Chromosomal protein]]		[[Category: Chromosomal protein]]
		+	[[Category: Coiled coil]]
	[[Category: Nucleosome]]		[[Category: Nucleosome]]
	[[Category: Nucleus]]		[[Category: Nucleus]]

OCA at 06:29, 7 February 2016

OCA — Sun, 07 Feb 2016 06:29:59 GMT

←Older revision		Revision as of 06:29, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='2wp1' size='340' side='right' caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2wp1' size='340' side='right' caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wp2\|2wp2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wp2\|2wp2]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://pdbe.org/2wp1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wp1 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2wp1" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Curtet, S]]		[[Category: Curtet, S]]
	[[Category: Gaucher, J]]		[[Category: Gaucher, J]]

OCA at 08:38, 24 December 2014

OCA — Wed, 24 Dec 2014 08:38:15 GMT

←Older revision		Revision as of 08:38, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BRDT_MOUSE BRDT_MOUSE]] Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids. Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.<ref>PMID:12861021</ref> <ref>PMID:22901802</ref> <ref>PMID:22922464</ref> <ref>PMID:22570411</ref> <ref>PMID:19794495</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Curtet, S.]]	+	[[Category: Curtet, S]]
-	[[Category: Gaucher, J.]]	+	[[Category: Gaucher, J]]
-	[[Category: Govin, J.]]	+	[[Category: Govin, J]]
-	[[Category: Hart, D J.]]	+	[[Category: Hart, D J]]
-	[[Category: Khochbin, S.]]	+	[[Category: Khochbin, S]]
-	[[Category: Krijgsveld, J.]]	+	[[Category: Krijgsveld, J]]
-	[[Category: Moriniere, J.]]	+	[[Category: Moriniere, J]]
-	[[Category: Mueller, C W.]]	+	[[Category: Mueller, C W]]
-	[[Category: Petosa, C.]]	+	[[Category: Petosa, C]]
-	[[Category: Rousseaux, S.]]	+	[[Category: Rousseaux, S]]
-	[[Category: Sadoul, K.]]	+	[[Category: Sadoul, K]]
-	[[Category: Soler-Lopez, M.]]	+	[[Category: Soler-Lopez, M]]
-	[[Category: Steuerwald, U.]]	+	[[Category: Steuerwald, U]]
-	[[Category: Vitte, A L.]]	+	[[Category: Vitte, A L]]
	[[Category: Acetyllysine brdt]]		[[Category: Acetyllysine brdt]]
	[[Category: Chromosomal protein]]		[[Category: Chromosomal protein]]

OCA at 05:56, 10 October 2014

OCA — Fri, 10 Oct 2014 05:56:37 GMT

←Older revision		Revision as of 05:56, 10 October 2014
Line 2:		Line 2:
	<StructureSection load='2wp1' size='340' side='right' caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2wp1' size='340' side='right' caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. <br>	+	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WP1 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wp2\|2wp2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wp2\|2wp2]]</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 23:
	Cooperative binding of two acetylation marks on a histone tail by a single bromodomain.,Moriniere J, Rousseaux S, Steuerwald U, Soler-Lopez M, Curtet S, Vitte AL, Govin J, Gaucher J, Sadoul K, Hart DJ, Krijgsveld J, Khochbin S, Muller CW, Petosa C Nature. 2009 Oct 1;461(7264):664-8. PMID:19794495<ref>PMID:19794495</ref>		Cooperative binding of two acetylation marks on a histone tail by a single bromodomain.,Moriniere J, Rousseaux S, Steuerwald U, Soler-Lopez M, Curtet S, Vitte AL, Govin J, Gaucher J, Sadoul K, Hart DJ, Krijgsveld J, Khochbin S, Muller CW, Petosa C Nature. 2009 Oct 1;461(7264):664-8. PMID:19794495<ref>PMID:19794495</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==

OCA at 08:38, 7 May 2014

OCA — Wed, 07 May 2014 08:38:20 GMT

←Older revision		Revision as of 08:38, 7 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2wp1.~~png~~\|~~left~~\|200px]]	+	==Structure of Brdt bromodomain 2 bound to an acetylated histone H3 peptide==
		+	<StructureSection load='2wp1' size='340' side='right' caption='[[2wp1]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2wp1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2wp2\|2wp2]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wp1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wp1 RCSB], [http://www.ebi.ac.uk/pdbsum/2wp1 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/2wp1_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A key step in many chromatin-related processes is the recognition of histone post-translational modifications by effector modules such as bromodomains and chromo-like domains of the Royal family. Whereas effector-mediated recognition of single post-translational modifications is well characterized, how the cell achieves combinatorial readout of histones bearing multiple modifications is poorly understood. One mechanism involves multivalent binding by linked effector modules. For example, the tandem bromodomains of human TATA-binding protein-associated factor-1 (TAF1) bind better to a diacetylated histone H4 tail than to monoacetylated tails, a cooperative effect attributed to each bromodomain engaging one acetyl-lysine mark. Here we report a distinct mechanism of combinatorial readout for the mouse TAF1 homologue Brdt, a testis-specific member of the BET protein family. Brdt associates with hyperacetylated histone H4 (ref. 7) and is implicated in the marked chromatin remodelling that follows histone hyperacetylation during spermiogenesis, the stage of spermatogenesis in which post-meiotic germ cells mature into fully differentiated sperm. Notably, we find that a single bromodomain (BD1) of Brdt is responsible for selectively recognizing histone H4 tails bearing two or more acetylation marks. The crystal structure of BD1 bound to a diacetylated H4 tail shows how two acetyl-lysine residues cooperate to interact with one binding pocket. Structure-based mutagenesis that reduces the selectivity of BD1 towards diacetylated tails destabilizes the association of Brdt with acetylated chromatin in vivo. Structural analysis suggests that other chromatin-associated proteins may be capable of a similar mode of ligand recognition, including yeast Bdf1, human TAF1 and human CBP/p300 (also known as CREBBP and EP300, respectively). Our findings describe a new mechanism for the combinatorial readout of histone modifications in which a single effector module engages two marks on a histone tail as a composite binding epitope.

-	<!--	+	Cooperative binding of two acetylation marks on a histone tail by a single bromodomain.,Moriniere J, Rousseaux S, Steuerwald U, Soler-Lopez M, Curtet S, Vitte AL, Govin J, Gaucher J, Sadoul K, Hart DJ, Krijgsveld J, Khochbin S, Muller CW, Petosa C Nature. 2009 Oct 1;461(7264):664-8. PMID:19794495<ref>PMID:19794495</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2wp1"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_2wp1\| PDB=2wp1 \| SCENE= }}~~	+

-	~~===Structure~~ of ~~Brdt bromodomain 2 bound to an acetylated histone H3 peptide===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_19794495}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 19794495 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_19794495}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2wp1]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019794495<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Curtet, S.]]		[[Category: Curtet, S.]]

OCA: Protected "2wp1" [edit=sysop:move=sysop]

OCA — Wed, 28 Mar 2012 06:44:11 GMT

Protected "2wp1" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:44, 28 March 2012

OCA at 06:44, 28 March 2012

OCA — Wed, 28 Mar 2012 06:44:09 GMT

←Older revision		Revision as of 06:44, 28 March 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2wp1.png\|left\|200px]]		[[Image:2wp1.png\|left\|200px]]

Line 10:		Line 9:
	{{STRUCTURE_2wp1\| PDB=2wp1 \| SCENE= }}		{{STRUCTURE_2wp1\| PDB=2wp1 \| SCENE= }}

-	===~~STRUCTURE OF BRDT BROMODOMAIN~~ 2 ~~BOUND TO AN ACETYLATED HISTONE~~ H3 ~~PEPTIDE~~===	+	===Structure of Brdt bromodomain 2 bound to an acetylated histone H3 peptide===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19794495}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19794495 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19794495}}

	==About this Structure==		==About this Structure==
-	~~2WP1~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA].	+	[[2wp1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WP1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:019794495</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Curtet, S.]]		[[Category: Curtet, S.]]
Line 30:		Line 38:
	[[Category: Steuerwald, U.]]		[[Category: Steuerwald, U.]]
	[[Category: Vitte, A L.]]		[[Category: Vitte, A L.]]
-	[[Category: Acetyllysine]]	+	[[Category: Acetyllysine brdt]]
-	~~[[Category: Brdt]]~~	+
-	~~[[Category: Bromodomain~~]]	+
	[[Category: Chromosomal protein]]		[[Category: Chromosomal protein]]
-	[[Category: Coiled coil]]
	[[Category: Nucleosome]]		[[Category: Nucleosome]]
	[[Category: Nucleus]]		[[Category: Nucleus]]
	[[Category: Transcription peptide complex]]		[[Category: Transcription peptide complex]]
	[[Category: Transcription regulation]]		[[Category: Transcription regulation]]
-	[[Category: Transcription/peptide complex]]	+	[[Category: Transcription-peptide complex]]
-		+
-	~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 7 13:23:22 2009''~~	+