2x3c - Revision history

OCA at 07:17, 9 August 2023

OCA — Wed, 09 Aug 2023 07:17:36 GMT

←Older revision		Revision as of 07:17, 9 August 2023
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	<StructureSection load='2x3c' size='340' side='right'caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='2x3c' size='340' side='right'caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"necromonas_achromogenes"_smith_1963 "necromonas~~ achromogenes~~" smith 1963~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X3C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_salmonicida_subsp._achromogenes Aeromonas salmonicida subsp. achromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X3C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.99Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2x3b\|2x3b]], [[2x3a\|2x3a]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3c OCA], [https://pdbe.org/2x3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x3c RCSB], [https://www.ebi.ac.uk/pdbsum/2x3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3c OCA], [https://pdbe.org/2x3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x3c RCSB], [https://www.ebi.ac.uk/pdbsum/2x3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3c ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8GMV9_AERSA Q8GMV9_AERSA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Necromonas~~ achromogenes ~~smith 1963]]~~	+	[[Category: Aeromonas salmonicida subsp. achromogenes]]
-	~~[[Category: Deuterolysin~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bogdanovic, X]]	+	[[Category: Bogdanovic X]]
-	[[Category: Hinrichs, W]]	+	[[Category: Hinrichs W]]
-	[[Category: Palm~~, G J~~]]	+	[[Category: Palm GJ]]
-	[[Category: Singh~~, R K]]~~	+	[[Category: Singh RK]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 16:49, 3 November 2021

OCA — Wed, 03 Nov 2021 16:49:03 GMT

←Older revision		Revision as of 16:49, 3 November 2021
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	<StructureSection load='2x3c' size='340' side='right'caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='2x3c' size='340' side='right'caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"necromonas_achromogenes"_smith_1963 "necromonas achromogenes" smith 1963]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2X3C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"necromonas_achromogenes"_smith_1963 "necromonas achromogenes" smith 1963]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X3C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x3b\|2x3b]], [[2x3a\|2x3a]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2x3b\|2x3b]], [[2x3a\|2x3a]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2x3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3c OCA], [~~http~~://pdbe.org/2x3c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2x3c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2x3c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3c OCA], [https://pdbe.org/2x3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x3c RCSB], [https://www.ebi.ac.uk/pdbsum/2x3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3c ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:45, 24 July 2019

OCA — Wed, 24 Jul 2019 07:45:32 GMT

←Older revision		Revision as of 07:45, 24 July 2019
Line 1:		Line 1:

	==AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase==		==AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase==
-	<StructureSection load='2x3c' size='340' side='right' caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>	+	<StructureSection load='2x3c' size='340' side='right'caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"necromonas_achromogenes"_smith_1963 "necromonas achromogenes" smith 1963]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X3C FirstGlance]. <br>		<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"necromonas_achromogenes"_smith_1963 "necromonas achromogenes" smith 1963]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X3C FirstGlance]. <br>
Line 24:		Line 24:
	[[Category: Necromonas achromogenes smith 1963]]		[[Category: Necromonas achromogenes smith 1963]]
	[[Category: Deuterolysin]]		[[Category: Deuterolysin]]
		+	[[Category: Large Structures]]
	[[Category: Bogdanovic, X]]		[[Category: Bogdanovic, X]]
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]

OCA at 06:38, 21 September 2016

OCA — Wed, 21 Sep 2016 06:38:10 GMT

←Older revision		Revision as of 06:38, 21 September 2016
Line 1:		Line 1:

-	==~~ASAP1 INACTIVE MUTANT~~ E294Q, ~~AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE~~==	+	==AsaP1 inactive mutant E294Q, an extracellular toxic zinc metalloendopeptidase==
	<StructureSection load='2x3c' size='340' side='right' caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='2x3c' size='340' side='right' caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 11:		Line 11:
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~Two mutants of the toxic extracellular zinc endopeptidase AsaP1 (AsaP1_E294Q and AsaP1_E294A) of~~ Aeromonas salmonicida subsp. achromogenes ~~were expressed in Escherichia coli and crystallized by~~ the ~~vapour-diffusion method~~. ~~Crystals were obtained using several precipitants and different protein concentrations. Protein crystals were found in a monoclinic (C2) as well as an orthorhombic (P2(1)~~2~~(1)2(1)) space group~~. ~~The crystals belonging to~~ the ~~monoclinic space group C2 had unit-cell parameters a = 103.4, b = 70.9, c = 54.9 A, beta = 109.3 degrees for AsaP1_E294A,~~ and ~~a = 98~~.~~5, b = 74.5, c = 54.7 A, beta = 112.4 degrees for AsaP1_E294Q. The unit-cell parameters~~ of ~~the orthorhombic crystal obtained for AsaP1_E294A were~~ a ~~= 57.9~~, ~~b = 60~~.~~2, c = 183.6~~ A~~. The crystals~~ of the ~~two different mutants diffracted X~~-~~rays beyond 2~~.~~0 A resolution~~.	+	The Gram-negative bacterium Aeromonas salmonicida is a fish pathogen for various fish species worldwide. Aeromonas salmonicida subsp. achromogenes produces the extracellular, toxic zinc endopeptidase AsaP1. Crystal structure analyses at 2.0 A resolution of two proteolytically inactive AsaP1 variants show the polypeptide folding of the protease domain and the propeptide domain. These first crystal structure analyses of a precursor of a deuterolysin-like aspzincin protease provide insights into propeptide function, and specific substrate binding. A lysine side chain of the propeptide binds in the hydrophobic S1'-pocket interacting with three carboxylate side chains. An AsaP1 variant with a lysine to alanine exchange identifies the chaperone function of the propeptide.

-	~~Crystallization and preliminary X-ray diffraction studies~~ of ~~AsaP1_E294A and AsaP1_E294Q, two inactive mutants~~ of the ~~toxic zinc metallopeptidase~~ AsaP1 ~~from Aeromonas salmonicida subsp. achromogenes~~.,Bogdanovic X, Singh RK~~, Hentschke J~~, Gudmundsdottir BK, Hinrichs W ~~Acta Crystallogr Sect F Struct Biol Cryst Commun~~. ~~2009 Jul 1;65(Pt, 7)~~:~~695~~-7. ~~Epub 2009 Jun 27~~. PMID:~~19574642~~<ref>PMID:~~19574642~~</ref>	+	Structural evidence of intramolecular propeptide inhibition of the aspzincin metalloendopeptidase AsaP1.,Bogdanovic X, Palm GJ, Schwenteit J, Singh RK, Gudmundsdottir BK, Hinrichs W FEBS Lett. 2016 Aug 16. doi: 10.1002/1873-3468.12356. PMID:27528449<ref>PMID:27528449</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 11:03, 11 August 2016

OCA — Thu, 11 Aug 2016 11:03:37 GMT

←Older revision		Revision as of 11:03, 11 August 2016
Line 1:		Line 1:
		+
	==ASAP1 INACTIVE MUTANT E294Q, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE==		==ASAP1 INACTIVE MUTANT E294Q, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE==
	<StructureSection load='2x3c' size='340' side='right' caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>		<StructureSection load='2x3c' size='340' side='right' caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aeromonas_salmonicida_subsp._achromogenes Aeromonas salmonicida subsp.~~ achromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X3C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"necromonas_achromogenes"_smith_1963 "necromonas achromogenes" smith 1963]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X3C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x3b\|2x3b]], [[2x3a\|2x3a]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x3b\|2x3b]], [[2x3a\|2x3a]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x3c RCSB], [http://www.ebi.ac.uk/pdbsum/2x3c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3c OCA], [http://pdbe.org/2x3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x3c RCSB], [http://www.ebi.ac.uk/pdbsum/2x3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3c ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2x3c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeromonas salmonicida subsp.~~ achromogenes]]	+	[[Category: Necromonas achromogenes smith 1963]]
	[[Category: Deuterolysin]]		[[Category: Deuterolysin]]
	[[Category: Bogdanovic, X]]		[[Category: Bogdanovic, X]]

OCA at 09:10, 25 January 2015

OCA — Sun, 25 Jan 2015 09:10:36 GMT

←Older revision		Revision as of 09:10, 25 January 2015
Line 22:		Line 22:
	[[Category: Aeromonas salmonicida subsp. achromogenes]]		[[Category: Aeromonas salmonicida subsp. achromogenes]]
	[[Category: Deuterolysin]]		[[Category: Deuterolysin]]
-	[[Category: Bogdanovic, X.]]	+	[[Category: Bogdanovic, X]]
-	[[Category: Hinrichs, W.]]	+	[[Category: Hinrichs, W]]
-	[[Category: Palm, G J.]]	+	[[Category: Palm, G J]]
-	[[Category: Singh, R K.]]	+	[[Category: Singh, R K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 09:32, 5 November 2014

OCA — Wed, 05 Nov 2014 09:32:12 GMT

←Older revision		Revision as of 09:32, 5 November 2014
Line 1:		Line 1:
-	[[~~Image:~~2x3c.~~png~~\|~~left~~\|~~200px~~]]	+	==ASAP1 INACTIVE MUTANT E294Q, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE==
		+	<StructureSection load='2x3c' size='340' side='right' caption='[[2x3c]], [[Resolution\|resolution]] 1.99Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2x3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_salmonicida_subsp._achromogenes Aeromonas salmonicida subsp. achromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X3C FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2x3b\|2x3b]], [[2x3a\|2x3a]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deuterolysin Deuterolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.39 3.4.24.39] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x3c RCSB], [http://www.ebi.ac.uk/pdbsum/2x3c PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Two mutants of the toxic extracellular zinc endopeptidase AsaP1 (AsaP1_E294Q and AsaP1_E294A) of Aeromonas salmonicida subsp. achromogenes were expressed in Escherichia coli and crystallized by the vapour-diffusion method. Crystals were obtained using several precipitants and different protein concentrations. Protein crystals were found in a monoclinic (C2) as well as an orthorhombic (P2(1)2(1)2(1)) space group. The crystals belonging to the monoclinic space group C2 had unit-cell parameters a = 103.4, b = 70.9, c = 54.9 A, beta = 109.3 degrees for AsaP1_E294A, and a = 98.5, b = 74.5, c = 54.7 A, beta = 112.4 degrees for AsaP1_E294Q. The unit-cell parameters of the orthorhombic crystal obtained for AsaP1_E294A were a = 57.9, b = 60.2, c = 183.6 A. The crystals of the two different mutants diffracted X-rays beyond 2.0 A resolution.

-	~~{{STRUCTURE_2x3c\| PDB=2x3c \| SCENE= }}~~	+	Crystallization and preliminary X-ray diffraction studies of AsaP1_E294A and AsaP1_E294Q, two inactive mutants of the toxic zinc metallopeptidase AsaP1 from Aeromonas salmonicida subsp. achromogenes.,Bogdanovic X, Singh RK, Hentschke J, Gudmundsdottir BK, Hinrichs W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt, 7):695-7. Epub 2009 Jun 27. PMID:19574642<ref>PMID:19574642</ref>

-	~~===ASAP1 INACTIVE MUTANT E294Q~~, ~~AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19574642}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2x3c]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aeromonas_salmonicida_subsp~~.~~_achromogenes Aeromonas salmonicida subsp. achromogenes]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2X3C OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019574642<~~/~~ref~~><~~ref group="xtra">PMID:018952802</ref><references group="xtra"~~/>	+
	[[Category: Aeromonas salmonicida subsp. achromogenes]]		[[Category: Aeromonas salmonicida subsp. achromogenes]]
	[[Category: Deuterolysin]]		[[Category: Deuterolysin]]

OCA at 13:21, 30 January 2013

OCA — Wed, 30 Jan 2013 13:21:34 GMT

←Older revision		Revision as of 13:21, 30 January 2013
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	[[Image:2x3c.png\|left\|200px]]		[[Image:2x3c.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_2x3c\| PDB=2x3c \| SCENE= }}		{{STRUCTURE_2x3c\| PDB=2x3c \| SCENE= }}

	===ASAP1 INACTIVE MUTANT E294Q, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE===		===ASAP1 INACTIVE MUTANT E294Q, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19574642}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19574642 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_19574642}}		{{ABSTRACT_PUBMED_19574642}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19574642~~</ref><ref group="xtra">PMID:~~18952802~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019574642</ref><ref group="xtra">PMID:018952802</ref><references group="xtra"/>
	[[Category: Aeromonas salmonicida subsp. achromogenes]]		[[Category: Aeromonas salmonicida subsp. achromogenes]]
	[[Category: Deuterolysin]]		[[Category: Deuterolysin]]
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	[[Category: Palm, G J.]]		[[Category: Palm, G J.]]
	[[Category: Singh, R K.]]		[[Category: Singh, R K.]]
		+	[[Category: Hydrolase]]

OCA at 18:34, 14 March 2011

OCA — Mon, 14 Mar 2011 18:34:47 GMT

←Older revision		Revision as of 18:34, 14 March 2011
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-	[[Image:2x3c.~~jpg~~\|left\|200px]]	+	[[Image:2x3c.png\|left\|200px]]

	<!--		<!--
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	===ASAP1 INACTIVE MUTANT E294Q, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE===		===ASAP1 INACTIVE MUTANT E294Q, AN EXTRACELLULAR TOXIC ZINC METALLOENDOPEPTIDASE===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19574642}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19574642 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19574642}}

	==About this Structure==		==About this Structure==

OCA: Protected "2x3c" [edit=sysop:move=sysop]

OCA — Wed, 02 Feb 2011 06:26:00 GMT

Protected "2x3c" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:26, 2 February 2011