2x80 - Revision history

OCA at 10:37, 16 August 2023

OCA — Wed, 16 Aug 2023 10:37:18 GMT

←Older revision		Revision as of 10:37, 16 August 2023
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	<StructureSection load='2x80' size='340' side='right'caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2x80' size='340' side='right'caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_14581 Atcc 14581~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X80 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>~~, ~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>[[1p0v\|1p0v]], [[1fag\|1fag]], [[1smi\|1smi]], [[2j1m\|2j1m]], [[1jme\|1jme]], [[2x7y\|2x7y]], [[1smj\|1smj]], [[2j4s\|2j4s]], [[2hpd\|2hpd]], [[1bvy\|1bvy]], [[1bu7\|1bu7]], [[1yqp\|1yqp]], [[2uwh\|2uwh]], [[2bmh\|2bmh]], [[1jpz\|1jpz]], [[1yqo\|1yqo]], [[1p0x\|1p0x]], [[1fah\|1fah]], [[1p0w\|1p0w]]</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [https://pdbe.org/2x80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [https://www.ebi.ac.uk/pdbsum/2x80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x80 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [https://pdbe.org/2x80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [https://www.ebi.ac.uk/pdbsum/2x80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x80 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~CPXB_BACME CPXB_BACME]~~] Functions as a fatty acid monooxygenase. Catalyzes hydroxylation of ~~medium and long-chain~~ fatty acids at omega-1, omega-2 and omega-3 positions, with optimum chain lengths of 12-16 carbons (lauric, myristic, and palmitic acids). ~~The~~ reductase domain ~~is required for~~ electron transfer from ~~NADP~~ to cytochrome P450.	+	[https://www.uniprot.org/uniprot/CPXB_PRIM2 CPXB_PRIM2] Functions as a fatty acid monooxygenase (PubMed:3106359, PubMed:1727637, PubMed:16566047, PubMed:7578081, PubMed:11695892, PubMed:14653735, PubMed:16403573, PubMed:18004886, PubMed:17077084, PubMed:17868686, PubMed:18298086, PubMed:18619466, PubMed:18721129, PubMed:19492389, PubMed:20180779, PubMed:21110374, PubMed:21875028). Catalyzes hydroxylation of fatty acids at omega-1, omega-2 and omega-3 positions (PubMed:1727637, PubMed:21875028). Shows activity toward medium and long-chain fatty acids, with optimum chain lengths of 12, 14 and 16 carbons (lauric, myristic, and palmitic acids). Able to metabolize some of these primary metabolites to secondary and tertiary products (PubMed:1727637). Marginal activity towards short chain lengths of 8-10 carbons (PubMed:1727637, PubMed:18619466). Hydroxylates highly branched fatty acids, which play an essential role in membrane fluidity regulation (PubMed:16566047). Also displays a NADPH-dependent reductase activity in the C-terminal domain, which allows electron transfer from NADPH to the heme iron of the cytochrome P450 N-terminal domain (PubMed:3106359, PubMed:1727637, PubMed:16566047, PubMed:7578081, PubMed:11695892, PubMed:14653735, PubMed:16403573, PubMed:18004886, PubMed:17077084, PubMed:17868686, PubMed:18298086, PubMed:18619466, PubMed:18721129, PubMed:19492389, PubMed:20180779, PubMed:21110374, PubMed:21875028). Involved in inactivation of quorum sensing signals of other competing bacteria by oxidazing efficiently acyl homoserine lactones (AHLs), molecules involved in quorum sensing signaling pathways, and their lactonolysis products acyl homoserines (AHs) (PubMed:18020460).<ref>PMID:11695892</ref> <ref>PMID:14653735</ref> <ref>PMID:16403573</ref> <ref>PMID:16566047</ref> <ref>PMID:17077084</ref> <ref>PMID:1727637</ref> <ref>PMID:17868686</ref> <ref>PMID:18004886</ref> <ref>PMID:18020460</ref> <ref>PMID:18298086</ref> <ref>PMID:18619466</ref> <ref>PMID:18721129</ref> <ref>PMID:19492389</ref> <ref>PMID:20180779</ref> <ref>PMID:21110374</ref> <ref>PMID:21875028</ref> <ref>PMID:3106359</ref> <ref>PMID:7578081</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 14581]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Unspecific monooxygenase~~]]	+	[[Category: Priestia megaterium]]
-	[[Category: Kuper, J]]	+	[[Category: Kuper J]]
-	[[Category: Roccatano, D]]	+	[[Category: Roccatano D]]
-	[[Category: Schwaneberg, U]]	+	[[Category: Schwaneberg U]]
-	[[Category: Wilmanns, M]]	+	[[Category: Wilmanns M]]
-	[[Category: Wong~~, T S]]~~	+	[[Category: Wong TS]]
-	~~[[Category: Dmso-inhibition]]~~	+
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:36, 10 November 2021

OCA — Wed, 10 Nov 2021 08:36:50 GMT

←Older revision		Revision as of 08:36, 10 November 2021
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	<StructureSection load='2x80' size='340' side='right'caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2x80' size='340' side='right'caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2X80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X80 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p0v\|1p0v]], [[1fag\|1fag]], [[1smi\|1smi]], [[2j1m\|2j1m]], [[1jme\|1jme]], [[2x7y\|2x7y]], [[1smj\|1smj]], [[2j4s\|2j4s]], [[2hpd\|2hpd]], [[1bvy\|1bvy]], [[1bu7\|1bu7]], [[1yqp\|1yqp]], [[2uwh\|2uwh]], [[2bmh\|2bmh]], [[1jpz\|1jpz]], [[1yqo\|1yqo]], [[1p0x\|1p0x]], [[1fah\|1fah]], [[1p0w\|1p0w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1p0v\|1p0v]], [[1fag\|1fag]], [[1smi\|1smi]], [[2j1m\|2j1m]], [[1jme\|1jme]], [[2x7y\|2x7y]], [[1smj\|1smj]], [[2j4s\|2j4s]], [[2hpd\|2hpd]], [[1bvy\|1bvy]], [[1bu7\|1bu7]], [[1yqp\|1yqp]], [[2uwh\|2uwh]], [[2bmh\|2bmh]], [[1jpz\|1jpz]], [[1yqo\|1yqo]], [[1p0x\|1p0x]], [[1fah\|1fah]], [[1p0w\|1p0w]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [~~http~~://pdbe.org/2x80 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2x80 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2x80 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [https://pdbe.org/2x80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [https://www.ebi.ac.uk/pdbsum/2x80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x80 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CPXB_BACME CPXB_BACME]] Functions as a fatty acid monooxygenase. Catalyzes hydroxylation of medium and long-chain fatty acids at omega-1, omega-2 and omega-3 positions, with optimum chain lengths of 12-16 carbons (lauric, myristic, and palmitic acids). The reductase domain is required for electron transfer from NADP to cytochrome P450.	+	[[https://www.uniprot.org/uniprot/CPXB_BACME CPXB_BACME]] Functions as a fatty acid monooxygenase. Catalyzes hydroxylation of medium and long-chain fatty acids at omega-1, omega-2 and omega-3 positions, with optimum chain lengths of 12-16 carbons (lauric, myristic, and palmitic acids). The reductase domain is required for electron transfer from NADP to cytochrome P450.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:45, 24 July 2019

OCA — Wed, 24 Jul 2019 07:45:55 GMT

←Older revision		Revision as of 07:45, 24 July 2019
Line 1:		Line 1:

	==P450 BM3 F87A in complex with DMSO==		==P450 BM3 F87A in complex with DMSO==
-	<StructureSection load='2x80' size='340' side='right' caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='2x80' size='340' side='right'caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X80 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X80 FirstGlance]. <br>
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	==See Also==		==See Also==
-	*[[Cytochrome P450\|Cytochrome P450]]	+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 14581]]		[[Category: Atcc 14581]]
		+	[[Category: Large Structures]]
	[[Category: Unspecific monooxygenase]]		[[Category: Unspecific monooxygenase]]
	[[Category: Kuper, J]]		[[Category: Kuper, J]]

OCA at 07:06, 5 August 2016

OCA — Fri, 05 Aug 2016 07:06:47 GMT

←Older revision		Revision as of 07:06, 5 August 2016
Line 1:		Line 1:
		+
	==P450 BM3 F87A in complex with DMSO==		==P450 BM3 F87A in complex with DMSO==
	<StructureSection load='2x80' size='340' side='right' caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2x80' size='340' side='right' caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_megaterium Bacillus megaterium~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14581 Atcc 14581]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X80 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p0v\|1p0v]], [[1fag\|1fag]], [[1smi\|1smi]], [[2j1m\|2j1m]], [[1jme\|1jme]], [[2x7y\|2x7y]], [[1smj\|1smj]], [[2j4s\|2j4s]], [[2hpd\|2hpd]], [[1bvy\|1bvy]], [[1bu7\|1bu7]], [[1yqp\|1yqp]], [[2uwh\|2uwh]], [[2bmh\|2bmh]], [[1jpz\|1jpz]], [[1yqo\|1yqo]], [[1p0x\|1p0x]], [[1fah\|1fah]], [[1p0w\|1p0w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p0v\|1p0v]], [[1fag\|1fag]], [[1smi\|1smi]], [[2j1m\|2j1m]], [[1jme\|1jme]], [[2x7y\|2x7y]], [[1smj\|1smj]], [[2j4s\|2j4s]], [[2hpd\|2hpd]], [[1bvy\|1bvy]], [[1bu7\|1bu7]], [[1yqp\|1yqp]], [[2uwh\|2uwh]], [[2bmh\|2bmh]], [[1jpz\|1jpz]], [[1yqo\|1yqo]], [[1p0x\|1p0x]], [[1fah\|1fah]], [[1p0w\|1p0w]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [http://www.ebi.ac.uk/pdbsum/2x80 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [http://pdbe.org/2x80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [http://www.ebi.ac.uk/pdbsum/2x80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2x80 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2x80" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus megaterium~~]]	+	[[Category: Atcc 14581]]
	[[Category: Unspecific monooxygenase]]		[[Category: Unspecific monooxygenase]]
	[[Category: Kuper, J]]		[[Category: Kuper, J]]

OCA at 00:05, 25 December 2014

OCA — Thu, 25 Dec 2014 00:05:49 GMT

←Older revision		Revision as of 00:05, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X80 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X80 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p0v\|1p0v]], [[1fag\|1fag]], [[1smi\|1smi]], [[2j1m\|2j1m]], [[1jme\|1jme]], [[2x7y\|2x7y]], [[1smj\|1smj]], [[2j4s\|2j4s]], [[2hpd\|2hpd]], [[1bvy\|1bvy]], [[1bu7\|1bu7]], [[1yqp\|1yqp]], [[2uwh\|2uwh]], [[2bmh\|2bmh]], [[1jpz\|1jpz]], [[1yqo\|1yqo]], [[1p0x\|1p0x]], [[1fah\|1fah]], [[1p0w\|1p0w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p0v\|1p0v]], [[1fag\|1fag]], [[1smi\|1smi]], [[2j1m\|2j1m]], [[1jme\|1jme]], [[2x7y\|2x7y]], [[1smj\|1smj]], [[2j4s\|2j4s]], [[2hpd\|2hpd]], [[1bvy\|1bvy]], [[1bu7\|1bu7]], [[1yqp\|1yqp]], [[2uwh\|2uwh]], [[2bmh\|2bmh]], [[1jpz\|1jpz]], [[1yqo\|1yqo]], [[1p0x\|1p0x]], [[1fah\|1fah]], [[1p0w\|1p0w]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [http://www.ebi.ac.uk/pdbsum/2x80 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [http://www.ebi.ac.uk/pdbsum/2x80 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CPXB_BACME CPXB_BACME]] Functions as a fatty acid monooxygenase. Catalyzes hydroxylation of medium and long-chain fatty acids at omega-1, omega-2 and omega-3 positions, with optimum chain lengths of 12-16 carbons (lauric, myristic, and palmitic acids). The reductase domain is required for electron transfer from NADP to cytochrome P450.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 27:
	[[Category: Bacillus megaterium]]		[[Category: Bacillus megaterium]]
	[[Category: Unspecific monooxygenase]]		[[Category: Unspecific monooxygenase]]
-	[[Category: Kuper, J.]]	+	[[Category: Kuper, J]]
-	[[Category: Roccatano, D.]]	+	[[Category: Roccatano, D]]
-	[[Category: Schwaneberg, U.]]	+	[[Category: Schwaneberg, U]]
-	[[Category: Wilmanns, M.]]	+	[[Category: Wilmanns, M]]
-	[[Category: Wong, T S.]]	+	[[Category: Wong, T S]]
	[[Category: Dmso-inhibition]]		[[Category: Dmso-inhibition]]
	[[Category: Electron transport]]		[[Category: Electron transport]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 06:51, 25 June 2014

OCA — Wed, 25 Jun 2014 06:51:28 GMT

←Older revision		Revision as of 06:51, 25 June 2014
Line 1:		Line 1:
-	[[~~Image:~~2x80.~~png~~\|~~left~~\|~~200px~~]]	+	==P450 BM3 F87A in complex with DMSO==
		+	<StructureSection load='2x80' size='340' side='right' caption='[[2x80]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2x80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2X80 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1p0v\|1p0v]], [[1fag\|1fag]], [[1smi\|1smi]], [[2j1m\|2j1m]], [[1jme\|1jme]], [[2x7y\|2x7y]], [[1smj\|1smj]], [[2j4s\|2j4s]], [[2hpd\|2hpd]], [[1bvy\|1bvy]], [[1bu7\|1bu7]], [[1yqp\|1yqp]], [[2uwh\|2uwh]], [[2bmh\|2bmh]], [[1jpz\|1jpz]], [[1yqo\|1yqo]], [[1p0x\|1p0x]], [[1fah\|1fah]], [[1p0w\|1p0w]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2x80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x80 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2x80 RCSB], [http://www.ebi.ac.uk/pdbsum/2x80 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Understanding the effects of organic co-solvents on protein structure and function is pivotal to engineering enzymes for biotransformation in non-aqueous solvents. The effects of DMSO on the catalytic activity of cytochrome P450 BM3 have previously been investigated and the importance of Phe87 in its organic co-solvent tolerance was identified. To probe the DMSO inactivation mechanism and the functional role of Phe87 in modulating the organic co-solvent tolerance of P450 BM3, the haem domain (Thr1-Leu455) of the F87A variant was cocrystallized in the presence of 14%(v/v) and 28%(v/v) DMSO. At both DMSO concentrations the protein retained the canonical structure of the P450 haem domain without any sign of partial or global unfolding. Interestingly, a DMSO molecule was found in the active site of both structures, with its O atom pointing towards the haem iron. The orientation of the DMSO molecule indicated a dynamic coordination process that was in competition with the active-site water molecule. The ability of the DMSO molecule to coordinate the haem iron is plausibly the main reason why P450 BM3 is inactivated at elevated DMSO concentrations. The data allowed an interesting comparison with the wild-type structures reported previously. A DMSO molecule was found when the wild-type protein was placed in 28%(v/v) DMSO, in which the DMSO molecule coordinated the haem iron directly via its S atom. Intriguingly, no DMSO molecule was observed at 14%(v/v) DMSO for the wild-type structure. These results suggested that the bulky phenyl side chain of Phe87 protects the haem from being accessed by the DMSO molecule and explains the higher tolerance of the wild-type enzyme towards organic co-solvents compared with its F87A variant.

-	~~{{STRUCTURE_2x80\| PDB=2x80 \| SCENE= }}~~	+	The role of active-site Phe87 in modulating the organic co-solvent tolerance of cytochrome P450 BM3 monooxygenase.,Kuper J, Tee KL, Wilmanns M, Roccatano D, Schwaneberg U, Wong TS Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Sep;68(Pt 9):1013-7. Epub , 2012 Aug 29. PMID:22949185<ref>PMID:22949185</ref>

-	~~===P450 BM3 F87A in complex with DMSO===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_22949185}}~~	+	==See Also==
-		+	*[[Cytochrome P450\|Cytochrome P450]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~2x80~~]] ~~is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA].~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus megaterium]]		[[Category: Bacillus megaterium]]
	[[Category: Unspecific monooxygenase]]		[[Category: Unspecific monooxygenase]]

OCA at 06:25, 19 September 2012

OCA — Wed, 19 Sep 2012 06:25:09 GMT

←Older revision		Revision as of 06:25, 19 September 2012
Line 5:		Line 5:
	===P450 BM3 F87A in complex with DMSO===		===P450 BM3 F87A in complex with DMSO===

		+	{{ABSTRACT_PUBMED_22949185}}

	==About this Structure==		==About this Structure==

OCA at 05:25, 12 September 2012

OCA — Wed, 12 Sep 2012 05:25:51 GMT

←Older revision		Revision as of 05:25, 12 September 2012
Line 1:		Line 1:
-	[[Image:2x80.~~jpg~~\|left\|200px]]	+	[[Image:2x80.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2x80", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2x80\| PDB=2x80 \| SCENE= }}		{{STRUCTURE_2x80\| PDB=2x80 \| SCENE= }}

-	===P450 BM3 F87A ~~IN COMPLEX WITH~~ DMSO===	+	===P450 BM3 F87A in complex with DMSO===


Line 21:		Line 15:
	[[Category: Wilmanns, M.]]		[[Category: Wilmanns, M.]]
	[[Category: Wong, T S.]]		[[Category: Wong, T S.]]
		+	[[Category: Dmso-inhibition]]
		+	[[Category: Electron transport]]
		+	[[Category: Metal-binding]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "2x80" [edit=sysop:move=sysop]

OCA — Wed, 23 Mar 2011 07:11:59 GMT

Protected "2x80" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:11, 23 March 2011

OCA at 07:11, 23 March 2011

OCA — Wed, 23 Mar 2011 07:11:52 GMT

←Older revision		Revision as of 07:11, 23 March 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2x80.jpg\|left\|200px]]

-	The ~~entry 2x80~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2x80", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2x80\| PDB=2x80 \| SCENE= }}

-	~~Authors: Kuper, J., Tuck-Seng, W., Roccatano, D., Wilmanns, M., Schwaneberg, U.~~	+	===P450 BM3 F87A IN COMPLEX WITH DMSO===

-	Description: P450 BM3 F87A in complex with DMSO

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Mar 17 09~~:06:~~43 2010''~~	+	==About this Structure==
		+	[[2x80]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X80 OCA].
		+	[[Category: Bacillus megaterium]]
		+	[[Category: Unspecific monooxygenase]]
		+	[[Category: Kuper, J.]]
		+	[[Category: Roccatano, D.]]
		+	[[Category: Schwaneberg, U.]]
		+	[[Category: Wilmanns, M.]]
		+	[[Category: Wong, T S.]]