2xh4 - Revision history

OCA at 10:31, 20 December 2023

OCA — Wed, 20 Dec 2023 10:31:24 GMT

←Older revision		Revision as of 10:31, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2xh4' size='340' side='right'caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2xh4' size='340' side='right'caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=2PG:2~~-~~PHOSPHOGLYCERIC+ACID'>2PG</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [https://pdbe.org/2xh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [https://www.ebi.ac.uk/pdbsum/2xh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xh4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [https://pdbe.org/2xh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [https://www.ebi.ac.uk/pdbsum/2xh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xh4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ENO1_YEAST ENO1_YEAST]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phosphopyruvate hydratase~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Hocker, B]]	+	[[Category: Hocker B]]
-	[[Category: Schreier, B]]	+	[[Category: Schreier B]]
-	~~[[Category: Enolase superfamily]]~~	+
-	~~[[Category: Gluconeogenesis]]~~	+
-	~~[[Category: Glycolysis]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Metal binding]]~~	+
-	~~[[Category: Tim-barrel~~]]	+

OCA at 10:52, 13 April 2022

OCA — Wed, 13 Apr 2022 10:52:44 GMT

←Older revision		Revision as of 10:52, 13 April 2022
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	<StructureSection load='2xh4' size='340' side='right'caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2xh4' size='340' side='right'caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [~~http~~://pdbe.org/2xh4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xh4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xh4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [https://pdbe.org/2xh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [https://www.ebi.ac.uk/pdbsum/2xh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xh4 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 15:14, 8 July 2020

OCA — Wed, 08 Jul 2020 15:14:18 GMT

←Older revision		Revision as of 15:14, 8 July 2020
Line 1:		Line 1:

	==Engineering the enolase active site pocket: Crystal structure of the S39A D321A mutant of yeast enolase 1==		==Engineering the enolase active site pocket: Crystal structure of the S39A D321A mutant of yeast enolase 1==
-	<StructureSection load='2xh4' size='340' side='right' caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='2xh4' size='340' side='right'caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [http://pdbe.org/2xh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [http://www.ebi.ac.uk/pdbsum/2xh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xh4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [http://pdbe.org/2xh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [http://www.ebi.ac.uk/pdbsum/2xh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xh4 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Enolase\|Enolase]]	+	*[[Enolase 3D structures\|Enolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
	[[Category: Hocker, B]]		[[Category: Hocker, B]]

OCA at 09:07, 3 October 2018

OCA — Wed, 03 Oct 2018 09:07:30 GMT

←Older revision		Revision as of 09:07, 3 October 2018
Line 1:		Line 1:

-	==~~ENGINEERING THE ENOLASE ACTIVE SITE POCKET~~: ~~CRYSTAL STRUCTURE OF THE~~ S39A D321A ~~MUTANT OF YEAST ENOLASE~~ 1==	+	==Engineering the enolase active site pocket: Crystal structure of the S39A D321A mutant of yeast enolase 1==
	<StructureSection load='2xh4' size='340' side='right' caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2xh4' size='340' side='right' caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xh/2xh4_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xh/2xh4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 16:49, 11 August 2016

OCA — Thu, 11 Aug 2016 16:49:58 GMT

←Older revision		Revision as of 16:49, 11 August 2016
Line 1:		Line 1:
		+
	==ENGINEERING THE ENOLASE ACTIVE SITE POCKET: CRYSTAL STRUCTURE OF THE S39A D321A MUTANT OF YEAST ENOLASE 1==		==ENGINEERING THE ENOLASE ACTIVE SITE POCKET: CRYSTAL STRUCTURE OF THE S39A D321A MUTANT OF YEAST ENOLASE 1==
	<StructureSection load='2xh4' size='340' side='right' caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='2xh4' size='340' side='right' caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [http://www.ebi.ac.uk/pdbsum/2xh4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [http://pdbe.org/2xh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [http://www.ebi.ac.uk/pdbsum/2xh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xh4 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xh4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xh4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
-	[[Category: Saccharomyces cerevisiae]]
	[[Category: Hocker, B]]		[[Category: Hocker, B]]
	[[Category: Schreier, B]]		[[Category: Schreier, B]]

OCA at 11:03, 20 January 2015

OCA — Tue, 20 Jan 2015 11:03:24 GMT

←Older revision		Revision as of 11:03, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [http://www.ebi.ac.uk/pdbsum/2xh4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [http://www.ebi.ac.uk/pdbsum/2xh4 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Hocker, B.]]	+	[[Category: Hocker, B]]
-	[[Category: Schreier, B.]]	+	[[Category: Schreier, B]]
	[[Category: Enolase superfamily]]		[[Category: Enolase superfamily]]
	[[Category: Gluconeogenesis]]		[[Category: Gluconeogenesis]]

OCA at 00:54, 2 October 2014

OCA — Thu, 02 Oct 2014 00:54:29 GMT

←Older revision		Revision as of 00:54, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2xh4.~~png~~\|~~left~~\|200px]]	+	==ENGINEERING THE ENOLASE ACTIVE SITE POCKET: CRYSTAL STRUCTURE OF THE S39A D321A MUTANT OF YEAST ENOLASE 1==
		+	<StructureSection load='2xh4' size='340' side='right' caption='[[2xh4]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XH4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PG:2-PHOSPHOGLYCERIC+ACID'>2PG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xh2\|2xh2]], [[2xgz\|2xgz]], [[1p48\|1p48]], [[1els\|1els]], [[1ebg\|1ebg]], [[3enl\|3enl]], [[1l8p\|1l8p]], [[4enl\|4enl]], [[2xh0\|2xh0]], [[1p43\|1p43]], [[5enl\|5enl]], [[1nel\|1nel]], [[2one\|2one]], [[2xh7\|2xh7]], [[6enl\|6enl]], [[1one\|1one]], [[7enl\|7enl]], [[2al2\|2al2]], [[1ebh\|1ebh]], [[2al1\|2al1]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xh4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xh4 RCSB], [http://www.ebi.ac.uk/pdbsum/2xh4 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xh/2xh4_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The glycolytic enzyme enolase catalyzes the reversible elimination of water from 2-phosphoglycerate (2-PGA) to form phosphoenolpyruvate (PEP). Two magnesium ions in the active site are thought to facilitate the reaction by activation of the C2 proton of 2-PGA and charge stabilization of the intermediate. The initial abstraction of a proton from a carboxylic acid is common to all members of the enolase superfamily, yet in all other known members of this superfamily, only one magnesium ion (MgI) per active site is sufficient to promote catalysis. We wanted to further investigate the importance of the second magnesium ion (MgII) for the catalytic mechanism of yeast enolase 1. Toward this end, we removed all MgII coordinating residues and replaced substrate-MgII interactions by introducing positively charged side chains. High-resolution crystal structures and activity assays show that the introduced positively charged side chains effectively prohibit MgII binding but fail to promote catalysis. We conclude that enolase is inactive without MgII, yet control mutants without additional positively charged side chains retain basal enolase activity through binding of magnesium to 2-PGA in an open active site without the help of MgII coordinating residues. Thus, we believe that ancestral enolase activity might have evolved in a member of the enolase superfamily that provides only the necessary catalytic residues and the binding site for MgI. Additionally, precatalytic binding of 2-PGA to the apo state of enolase was observed.

-	~~{{STRUCTURE_2xh4\| PDB=2xh4 \| SCENE= }}~~	+	Engineering the Enolase Magnesium II Binding Site: Implications for Its Evolution.,Schreier B, Hocker B Biochemistry. 2010 Aug 17. PMID:20690637<ref>PMID:20690637</ref>

-	~~===ENGINEERING THE ENOLASE ACTIVE SITE POCKET: CRYSTAL STRUCTURE OF THE S39A D321A MUTANT OF YEAST ENOLASE 1===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20690637}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2xh4]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA].~~	+

	==See Also==		==See Also==
	*[[Enolase\|Enolase]]		*[[Enolase\|Enolase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020690637<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]

OCA at 12:32, 20 October 2012

OCA — Sat, 20 Oct 2012 12:32:57 GMT

←Older revision		Revision as of 12:32, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2xh4]] is a 4 chain structure ~~of [[Enolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA].	+	[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA].

	==See Also==		==See Also==

OCA at 09:05, 26 July 2012

OCA — Thu, 26 Jul 2012 09:05:02 GMT

←Older revision		Revision as of 09:05, 26 July 2012
Line 1:		Line 1:
	[[Image:2xh4.png\|left\|200px]]		[[Image:2xh4.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xh4", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xh4\| PDB=2xh4 \| SCENE= }}		{{STRUCTURE_2xh4\| PDB=2xh4 \| SCENE= }}

	===ENGINEERING THE ENOLASE ACTIVE SITE POCKET: CRYSTAL STRUCTURE OF THE S39A D321A MUTANT OF YEAST ENOLASE 1===		===ENGINEERING THE ENOLASE ACTIVE SITE POCKET: CRYSTAL STRUCTURE OF THE S39A D321A MUTANT OF YEAST ENOLASE 1===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20690637}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20690637 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20690637}}		{{ABSTRACT_PUBMED_20690637}}

	==About this Structure==		==About this Structure==
-	[[2xh4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA].	+	[[2xh4]] is a 4 chain structure of [[Enolase]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XH4 OCA].
		+
		+	==See Also==
		+	*[[Enolase\|Enolase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20690637~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020690637</ref><references group="xtra"/>
	[[Category: Phosphopyruvate hydratase]]		[[Category: Phosphopyruvate hydratase]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Hocker, B.]]		[[Category: Hocker, B.]]
	[[Category: Schreier, B.]]		[[Category: Schreier, B.]]
		+	[[Category: Enolase superfamily]]
		+	[[Category: Gluconeogenesis]]
		+	[[Category: Glycolysis]]
		+	[[Category: Lyase]]
		+	[[Category: Metal binding]]
		+	[[Category: Tim-barrel]]

OCA: Protected "2xh4" [edit=sysop:move=sysop]

OCA — Thu, 21 Apr 2011 09:52:12 GMT

Protected "2xh4" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:52, 21 April 2011