2xhz - Revision history

OCA at 10:32, 20 December 2023

OCA — Wed, 20 Dec 2023 10:32:10 GMT

←Older revision		Revision as of 10:32, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2xhz' size='340' side='right'caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2xhz' size='340' side='right'caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>~~[https://en.wikipedia.org/wiki/Arabinose~~-~~5-phosphate_isomerase Arabinose-5-phosphate isomerase]~~, ~~with EC number~~ [~~https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [https://pdbe.org/2xhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [https://www.ebi.ac.uk/pdbsum/2xhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [https://pdbe.org/2xhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [https://www.ebi.ac.uk/pdbsum/2xhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/KDSD_ECOLI KDSD_ECOLI]] Involved in the biosynthesis of 3-deoxy-D-manno-octulosonate (KDO), a unique 8-carbon sugar component of lipopolysaccharides (LPSs). KdsD is not essential in the KDO biosynthesis and can be substituted by GutQ. Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P).<ref>PMID:12805358</ref> <ref>PMID:16199563</ref> <ref>PMID:16765569</ref>	+	[https://www.uniprot.org/uniprot/KDSD_ECOLI KDSD_ECOLI] Involved in the biosynthesis of 3-deoxy-D-manno-octulosonate (KDO), a unique 8-carbon sugar component of lipopolysaccharides (LPSs). KdsD is not essential in the KDO biosynthesis and can be substituted by GutQ. Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P).<ref>PMID:12805358</ref> <ref>PMID:16199563</ref> <ref>PMID:16765569</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Arabinose-5-phosphate isomerase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bolognesi, M]]	+	[[Category: Bolognesi M]]
-	[[Category: Deho, G]]	+	[[Category: Deho G]]
-	[[Category: Gourlay~~, L J~~]]	+	[[Category: Gourlay LJ]]
-	[[Category: Nardini, M]]	+	[[Category: Nardini M]]
-	[[Category: Polissi, A]]	+	[[Category: Polissi A]]
-	[[Category: Sommaruga, S]]	+	[[Category: Sommaruga S]]
-	[[Category: Sperandeo, P]]	+	[[Category: Sperandeo P]]
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Lipopolysaccharide biogenesis~~]]	+

OCA at 10:53, 13 April 2022

OCA — Wed, 13 Apr 2022 10:53:20 GMT

←Older revision		Revision as of 10:53, 13 April 2022
Line 3:		Line 3:
	<StructureSection load='2xhz' size='340' side='right'caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2xhz' size='340' side='right'caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Arabinose-5-phosphate_isomerase Arabinose-5-phosphate isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Arabinose-5-phosphate_isomerase Arabinose-5-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [~~http~~://pdbe.org/2xhz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xhz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [https://pdbe.org/2xhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [https://www.ebi.ac.uk/pdbsum/2xhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/KDSD_ECOLI KDSD_ECOLI]] Involved in the biosynthesis of 3-deoxy-D-manno-octulosonate (KDO), a unique 8-carbon sugar component of lipopolysaccharides (LPSs). KdsD is not essential in the KDO biosynthesis and can be substituted by GutQ. Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P).<ref>PMID:12805358</ref> <ref>PMID:16199563</ref> <ref>PMID:16765569</ref>	+	[[https://www.uniprot.org/uniprot/KDSD_ECOLI KDSD_ECOLI]] Involved in the biosynthesis of 3-deoxy-D-manno-octulosonate (KDO), a unique 8-carbon sugar component of lipopolysaccharides (LPSs). KdsD is not essential in the KDO biosynthesis and can be substituted by GutQ. Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P).<ref>PMID:12805358</ref> <ref>PMID:16199563</ref> <ref>PMID:16765569</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:15, 8 July 2020

OCA — Wed, 08 Jul 2020 15:15:07 GMT

←Older revision		Revision as of 15:15, 8 July 2020
Line 1:		Line 1:

	==Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography==		==Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography==
-	<StructureSection load='2xhz' size='340' side='right' caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='2xhz' size='340' side='right'caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinose-5-phosphate_isomerase Arabinose-5-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinose-5-phosphate_isomerase Arabinose-5-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [http://pdbe.org/2xhz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [http://www.ebi.ac.uk/pdbsum/2xhz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [http://pdbe.org/2xhz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [http://www.ebi.ac.uk/pdbsum/2xhz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 24:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Arabinose-5-phosphate isomerase]]		[[Category: Arabinose-5-phosphate isomerase]]
		+	[[Category: Large Structures]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
	[[Category: Deho, G]]		[[Category: Deho, G]]

OCA at 09:33, 3 October 2018

OCA — Wed, 03 Oct 2018 09:33:09 GMT

←Older revision		Revision as of 09:33, 3 October 2018
Line 1:		Line 1:

-	==~~PROBING THE ACTIVE SITE OF THE SUGAR ISOMERASE DOMAIN FROM~~ E. ~~COLI ARABINOSE~~-5-~~PHOSPHATE ISOMERASE VIA~~ X-~~RAY CRYSTALLOGRAPHY~~==	+	==Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography==
	<StructureSection load='2xhz' size='340' side='right' caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2xhz' size='340' side='right' caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 19:36, 4 August 2016

OCA — Thu, 04 Aug 2016 19:36:49 GMT

←Older revision		Revision as of 19:36, 4 August 2016
Line 1:		Line 1:
		+
	==PROBING THE ACTIVE SITE OF THE SUGAR ISOMERASE DOMAIN FROM E. COLI ARABINOSE-5-PHOSPHATE ISOMERASE VIA X-RAY CRYSTALLOGRAPHY==		==PROBING THE ACTIVE SITE OF THE SUGAR ISOMERASE DOMAIN FROM E. COLI ARABINOSE-5-PHOSPHATE ISOMERASE VIA X-RAY CRYSTALLOGRAPHY==
	<StructureSection load='2xhz' size='340' side='right' caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='2xhz' size='340' side='right' caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinose-5-phosphate_isomerase Arabinose-5-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinose-5-phosphate_isomerase Arabinose-5-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [http://www.ebi.ac.uk/pdbsum/2xhz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [http://pdbe.org/2xhz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [http://www.ebi.ac.uk/pdbsum/2xhz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xhz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Arabinose-5-phosphate isomerase]]		[[Category: Arabinose-5-phosphate isomerase]]
-	[[Category: Escherichia coli]]
	[[Category: Bolognesi, M]]		[[Category: Bolognesi, M]]
	[[Category: Deho, G]]		[[Category: Deho, G]]

OCA at 18:50, 24 December 2014

OCA — Wed, 24 Dec 2014 18:50:57 GMT

←Older revision		Revision as of 18:50, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [http://www.ebi.ac.uk/pdbsum/2xhz PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [http://www.ebi.ac.uk/pdbsum/2xhz PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KDSD_ECOLI KDSD_ECOLI]] Involved in the biosynthesis of 3-deoxy-D-manno-octulosonate (KDO), a unique 8-carbon sugar component of lipopolysaccharides (LPSs). KdsD is not essential in the KDO biosynthesis and can be substituted by GutQ. Catalyzes the reversible aldol-ketol isomerization between D-ribulose 5-phosphate (Ru5P) and D-arabinose 5-phosphate (A5P).<ref>PMID:12805358</ref> <ref>PMID:16199563</ref> <ref>PMID:16765569</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:38, 26 November 2014

OCA — Wed, 26 Nov 2014 08:38:03 GMT

←Older revision		Revision as of 08:38, 26 November 2014
Line 1:		Line 1:
-	[[~~Image~~:2xhz.~~png\|left\|200px~~]]	+	==PROBING THE ACTIVE SITE OF THE SUGAR ISOMERASE DOMAIN FROM E. COLI ARABINOSE-5-PHOSPHATE ISOMERASE VIA X-RAY CRYSTALLOGRAPHY==
		+	<StructureSection load='2xhz' size='340' side='right' caption='[[2xhz]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xhz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XHZ FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arabinose-5-phosphate_isomerase Arabinose-5-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.13 5.3.1.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xhz RCSB], [http://www.ebi.ac.uk/pdbsum/2xhz PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lipopolysaccharide (LPS) biosynthesis represents an underexploited target pathway for novel antimicrobial development to combat the emergence of multidrug-resistant bacteria. A key player in LPS synthesis is the enzyme D-arabinose-5-phosphate isomerase (API), which catalyzes the reversible isomerization of D-ribulose-5-phosphate to D-arabinose-5-phosphate, a precursor of 3-deoxy-D-manno-octulosonate that is an essential residue of the LPS inner core. API is composed of two main domains: an N-terminal sugar isomerase domain (SIS) and a pair of cystathionine-beta-synthase domains of unknown function. As the three-dimensional structure of an enzyme is a prerequisite for the rational development of novel inhibitors, we present here the crystal structure of the SIS domain of a catalytic mutant (K59A) of E. coli D-arabinose-5-phosphate isomerase at 2.6-A resolution. Our structural analyses and comparisons made with other SIS domains highlight several potentially important active site residues. In particular, the crystal structure allowed us to identify a previously unpredicted His residue (H88) located at the mouth of the active site cavity as a possible catalytic residue. On the basis of such structural data, subsequently supported by biochemical and mutational experiments, we confirm the catalytic role of H88, which appears to be a generally conserved residue among two-domain isomerases.

-	~~{{STRUCTURE_2xhz\| PDB=2xhz \| SCENE= }}~~	+	Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography.,Gourlay LJ, Sommaruga S, Nardini M, Sperandeo P, Deho G, Polissi A, Bolognesi M Protein Sci. 2010 Dec;19(12):2430-9. doi: 10.1002/pro.525. PMID:20954237<ref>PMID:20954237</ref>

-	~~===PROBING THE ACTIVE SITE OF THE SUGAR ISOMERASE DOMAIN FROM E. COLI ARABINOSE-5-PHOSPHATE ISOMERASE VIA X-RAY CRYSTALLOGRAPHY===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20954237}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2xhz]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XHZ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:020954237<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arabinose-5-phosphate isomerase]]		[[Category: Arabinose-5-phosphate isomerase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Bolognesi, M.]]	+	[[Category: Bolognesi, M]]
-	[[Category: Deho, G.]]	+	[[Category: Deho, G]]
-	[[Category: Gourlay, L J.]]	+	[[Category: Gourlay, L J]]
-	[[Category: Nardini, M.]]	+	[[Category: Nardini, M]]
-	[[Category: Polissi, A.]]	+	[[Category: Polissi, A]]
-	[[Category: Sommaruga, S.]]	+	[[Category: Sommaruga, S]]
-	[[Category: Sperandeo, P.]]	+	[[Category: Sperandeo, P]]
	[[Category: Isomerase]]		[[Category: Isomerase]]
	[[Category: Lipopolysaccharide biogenesis]]		[[Category: Lipopolysaccharide biogenesis]]

OCA at 14:22, 20 February 2013

OCA — Wed, 20 Feb 2013 14:22:07 GMT

←Older revision		Revision as of 14:22, 20 February 2013
Line 1:		Line 1:
	[[Image:2xhz.png\|left\|200px]]		[[Image:2xhz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xhz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xhz\| PDB=2xhz \| SCENE= }}		{{STRUCTURE_2xhz\| PDB=2xhz \| SCENE= }}

	===PROBING THE ACTIVE SITE OF THE SUGAR ISOMERASE DOMAIN FROM E. COLI ARABINOSE-5-PHOSPHATE ISOMERASE VIA X-RAY CRYSTALLOGRAPHY===		===PROBING THE ACTIVE SITE OF THE SUGAR ISOMERASE DOMAIN FROM E. COLI ARABINOSE-5-PHOSPHATE ISOMERASE VIA X-RAY CRYSTALLOGRAPHY===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20954237}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20954237 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20954237}}		{{ABSTRACT_PUBMED_20954237}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20954237~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020954237</ref><references group="xtra"/>
	[[Category: Arabinose-5-phosphate isomerase]]		[[Category: Arabinose-5-phosphate isomerase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
Line 32:		Line 21:
	[[Category: Sommaruga, S.]]		[[Category: Sommaruga, S.]]
	[[Category: Sperandeo, P.]]		[[Category: Sperandeo, P.]]
		+	[[Category: Isomerase]]
		+	[[Category: Lipopolysaccharide biogenesis]]

OCA at 04:31, 13 April 2011

OCA — Wed, 13 Apr 2011 04:31:13 GMT

←Older revision		Revision as of 04:31, 13 April 2011
Line 1:		Line 1:
-	[[Image:2xhz.~~jpg~~\|left\|200px]]	+	[[Image:2xhz.png\|left\|200px]]

	<!--		<!--

OCA: Protected "2xhz" [edit=sysop:move=sysop]

OCA — Wed, 26 Jan 2011 12:59:28 GMT

Protected "2xhz" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:59, 26 January 2011