2xjf - Revision history

OCA at 10:32, 20 December 2023

OCA — Wed, 20 Dec 2023 10:32:51 GMT

←Older revision		Revision as of 10:32, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2xjf' size='340' side='right'caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2xjf' size='340' side='right'caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DG2:(2R)~~-2~~,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene>, <scene name='pdbligand=DG2:(2R)-2,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;~~'>~~[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2jcm\|2jcm]], [[2xcx\|2xcx]], [[2xje\|2xje]], [[2xcw\|2xcw]], [[2xjb\|2xjb]], [[2xjc\|2xjc]]~~</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [https://pdbe.org/2xjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [https://www.ebi.ac.uk/pdbsum/2xjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [https://pdbe.org/2xjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [https://www.ebi.ac.uk/pdbsum/2xjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjf ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>	+	[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.	+	[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~5'-nucleotidase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nordlund, P]]	+	[[Category: Nordlund P]]
-	[[Category: Wallden, K]]	+	[[Category: Wallden K]]
-	~~[[Category: Allosteric enzyme]]~~	+
-	~~[[Category: Cn-ii]]~~	+
-	~~[[Category: Gmp-imp specific nucleotidase]]~~	+
-	~~[[Category: High km 5-prime nucleotidase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Nt5c2]]~~	+
-	~~[[Category: Nucleotide metabolism]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Phosphoprotein~~]]	+

OCA at 10:54, 13 April 2022

OCA — Wed, 13 Apr 2022 10:54:12 GMT

OCA at 15:16, 8 July 2020

OCA — Wed, 08 Jul 2020 15:16:11 GMT

←Older revision		Revision as of 15:16, 8 July 2020
Line 1:		Line 1:

	==Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II with a covalently modified Asn52==		==Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II with a covalently modified Asn52==
-	<StructureSection load='2xjf' size='340' side='right' caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='2xjf' size='340' side='right'caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XJF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DG2:(2R)-2,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DG2:(2R)-2,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2jcm\|2jcm]], [[2xcx\|2xcx]], [[2xje\|2xje]], [[2xcw\|2xcw]], [[2xjb\|2xjb]], [[2xjc\|2xjc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2jcm\|2jcm]], [[2xcx\|2xcx]], [[2xje\|2xje]], [[2xcw\|2xcw]], [[2xjb\|2xjb]], [[2xjc\|2xjc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [http://pdbe.org/2xjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [http://www.ebi.ac.uk/pdbsum/2xjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [http://pdbe.org/2xjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [http://www.ebi.ac.uk/pdbsum/2xjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjf ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 29:		Line 29:
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Nordlund, P]]		[[Category: Nordlund, P]]
	[[Category: Wallden, K]]		[[Category: Wallden, K]]

OCA at 06:26, 26 July 2016

OCA — Tue, 26 Jul 2016 06:26:49 GMT

←Older revision		Revision as of 06:26, 26 July 2016
Line 1:		Line 1:
-	==~~CRYSTAL STRUCTURE OF THE~~ D52N ~~VARIANT OF CYTOSOLIC~~ 5'-~~NUCLEOTIDASE~~ II ~~WITH A COVALENTLY MODIFIED ASN52~~==	+
		+	==Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II with a covalently modified Asn52==
	<StructureSection load='2xjf' size='340' side='right' caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2xjf' size='340' side='right' caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DG2:2,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DG2:(2R)-2,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2jcm\|2jcm]], [[2xcx\|2xcx]], [[2xje\|2xje]], [[2xcw\|2xcw]], [[2xjb\|2xjb]], [[2xjc\|2xjc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2jcm\|2jcm]], [[2xcx\|2xcx]], [[2xje\|2xje]], [[2xcw\|2xcw]], [[2xjb\|2xjb]], [[2xjc\|2xjc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [http://www.ebi.ac.uk/pdbsum/2xjf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [http://pdbe.org/2xjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [http://www.ebi.ac.uk/pdbsum/2xjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjf ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 21:		Line 22:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xjf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Nordlund, P]]		[[Category: Nordlund, P]]
	[[Category: Wallden, K]]		[[Category: Wallden, K]]

OCA at 13:52, 17 December 2014

OCA — Wed, 17 Dec 2014 13:52:40 GMT

←Older revision		Revision as of 13:52, 17 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_2xjf\| PDB=2xjf \| SCENE= }}~~	+	==CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II WITH A COVALENTLY MODIFIED ASN52==
-	===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II WITH A COVALENTLY MODIFIED ASN52===	+	<StructureSection load='2xjf' size='340' side='right' caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21396942}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DG2:2,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2jcm\|2jcm]], [[2xcx\|2xcx]], [[2xje\|2xje]], [[2xcw\|2xcw]], [[2xjb\|2xjb]], [[2xjc\|2xjc]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [http://www.ebi.ac.uk/pdbsum/2xjf PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytosolic 5'-nucleotidase II (cN-II) catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates and participates in the regulation of purine nucleotide pools within the cell. It interferes with the phosphorylation-dependent activation of nucleoside analogues used in the treatment of cancer and viral diseases. It is allosterically activated by a number of phosphate-containing cellular metabolites such as ATP, diadenosine polyphosphates, and 2,3-bisphosphoglycerate, which couple its activity with the metabolic state of the cell. We present seven high-resolution structures of human cN-II, including a ligand-free form and complexes with various substrates and effectors. These structures reveal the structural basis for the allosteric activation of cN-II, uncovering a mechanism where an effector-induced disorder-to-order transition generates rearrangements within the catalytic site and the subsequent coordination of the catalytically essential magnesium. Central to the activation is the large transition of the catalytically essential Asp356. This study also provides the structural basis for the substrate specificity of cN-II, where Arg202, Asp206, and Phe157 seem to be important residues for purine/pyrimidine selectivity. These structures provide a comprehensive structural basis for the design of cN-II inhibitors. They also contribute to the understanding of how the nucleotide salvage pathway is regulated at a molecular level.

-	~~==About this Structure==~~	+	Structural Basis for the Allosteric Regulation and Substrate Recognition of Human Cytosolic 5'-Nucleotidase II.,Wallden K, Nordlund P J Mol Biol. 2011 May 13;408(4):684-96. Epub 2011 Mar 17. PMID:21396942<ref>PMID:21396942</ref>
-	~~[[2xjf]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http~~:~~//oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=2XJF OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	<~~ref group="xtra">PMID:021396942<~~/~~ref~~><~~references group="xtra"~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Nordlund, P.]]	+	[[Category: Nordlund, P]]
-	[[Category: Wallden, K.]]	+	[[Category: Wallden, K]]
	[[Category: Allosteric enzyme]]		[[Category: Allosteric enzyme]]
	[[Category: Cn-ii]]		[[Category: Cn-ii]]

OCA at 10:46, 13 March 2013

OCA — Wed, 13 Mar 2013 10:46:57 GMT

←Older revision		Revision as of 10:46, 13 March 2013
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-	[[Image:2xjf.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xjf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xjf\| PDB=2xjf \| SCENE= }}		{{STRUCTURE_2xjf\| PDB=2xjf \| SCENE= }}
-
	===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II WITH A COVALENTLY MODIFIED ASN52===		===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II WITH A COVALENTLY MODIFIED ASN52===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21396942}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21396942 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21396942}}		{{ABSTRACT_PUBMED_21396942}}

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	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21396942~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021396942</ref><references group="xtra"/>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Nordlund, P.]]		[[Category: Nordlund, P.]]
	[[Category: Wallden, K.]]		[[Category: Wallden, K.]]
		+	[[Category: Allosteric enzyme]]
		+	[[Category: Cn-ii]]
		+	[[Category: Gmp-imp specific nucleotidase]]
		+	[[Category: High km 5-prime nucleotidase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Metal-binding]]
		+	[[Category: Nt5c2]]
		+	[[Category: Nucleotide metabolism]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Phosphoprotein]]

OCA at 06:30, 27 April 2011

OCA — Wed, 27 Apr 2011 06:30:26 GMT

←Older revision		Revision as of 06:30, 27 April 2011
Line 1:		Line 1:
-	[[Image:2xjf.~~jpg~~\|left\|200px]]	+	[[Image:2xjf.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II WITH A COVALENTLY MODIFIED ASN52===		===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II WITH A COVALENTLY MODIFIED ASN52===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21396942}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21396942 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21396942}}

	==About this Structure==		==About this Structure==
	[[2xjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA].		[[2xjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21396942</ref><references group="xtra"/>
	[[Category: 5'-nucleotidase]]		[[Category: 5'-nucleotidase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Nordlund, P.]]		[[Category: Nordlund, P.]]
	[[Category: Wallden, K.]]		[[Category: Wallden, K.]]

OCA at 08:27, 16 March 2011

OCA — Wed, 16 Mar 2011 08:27:23 GMT

←Older revision		Revision as of 08:27, 16 March 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:2xjf.jpg\|left\|200px]]

-	The ~~entry 2xjf~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2xjf", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2xjf\| PDB=2xjf \| SCENE= }}

-	~~Authors: Wallden, K., Nordlund, P.~~	+	===CRYSTAL STRUCTURE OF THE D52N VARIANT OF CYTOSOLIC 5'-NUCLEOTIDASE II WITH A COVALENTLY MODIFIED ASN52===

-	~~Description: Crystal~~ structure ~~of the D52N variant of cytosolic~~ 5'-nucleotidase ~~II with a covalently modified Asn52~~	+
		+	==About this Structure==
		+	[[2xjf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA].
		+	[[Category: 5'-nucleotidase]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Nordlund, P.]]
		+	[[Category: Wallden, K.]]

OCA: Protected "2xjf" [edit=sysop:move=sysop]

OCA — Wed, 12 Jan 2011 04:40:15 GMT

Protected "2xjf" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:40, 12 January 2011

OCA at 04:40, 12 January 2011

OCA — Wed, 12 Jan 2011 04:40:14 GMT

←Older revision		Revision as of 04:40, 12 January 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2xjf is ON HOLD	+	The entry 2xjf is ON HOLD until Paper Publication

	Authors: Wallden, K., Nordlund, P.		Authors: Wallden, K., Nordlund, P.

	Description: Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II with a covalently modified Asn52		Description: Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II with a covalently modified Asn52
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 16:01:22 2010''

←Older revision		Revision as of 10:54, 13 April 2022
Line 3:		Line 3:
	<StructureSection load='2xjf' size='340' side='right'caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='2xjf' size='340' side='right'caption='[[2xjf]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DG2:(2R)-2,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DG2:(2R)-2,3-DIPHOSPHOGLYCERIC+ACID'>DG2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=A5N:O-CARBOXY-4-IMINO-L-HOMOSERINE'>A5N</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2jcm\|2jcm]], [[2xcx\|2xcx]], [[2xje\|2xje]], [[2xcw\|2xcw]], [[2xjb\|2xjb]], [[2xjc\|2xjc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2jc9\|2jc9]], [[2xcv\|2xcv]], [[2xjd\|2xjd]], [[2j2c\|2j2c]], [[2jcm\|2jcm]], [[2xcx\|2xcx]], [[2xje\|2xje]], [[2xcw\|2xcw]], [[2xjb\|2xjb]], [[2xjc\|2xjc]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/5'-nucleotidase 5'-nucleotidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.5 3.1.3.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [~~http~~://pdbe.org/2xjf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xjf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjf OCA], [https://pdbe.org/2xjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjf RCSB], [https://www.ebi.ac.uk/pdbsum/2xjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjf ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>	+	[[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] Autosomal recessive spastic paraplegia type 45. The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:24482476</ref>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.	+	[[https://www.uniprot.org/uniprot/5NTC_HUMAN 5NTC_HUMAN]] May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==