2xjt - Revision history

OCA at 07:11, 1 May 2024

OCA — Wed, 01 May 2024 07:11:19 GMT

←Older revision		Revision as of 07:11, 1 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/FLO5_YEAST FLO5_YEAST] Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.		[https://www.uniprot.org/uniprot/FLO5_YEAST FLO5_YEAST] Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In the budding yeast Saccharomyces cerevisiae, self-recognition and the thereby promoted aggregation of thousands of cells into protective flocs is mediated by a family of cell-surface adhesins, the flocculins (Flo). Based on this social behavior FLO genes fulfill the definition of "greenbeard" genes, which direct cooperation toward other carriers of the same gene. The process of flocculation plays an eminent role in the food industry for the production of beer and wine. However, the precise mode of flocculin-mediated surface recognition and the exact structure of cognate ligands have remained elusive. Here, we present structures of the adhesion domain of a flocculin complexed to its cognate ligands derived from yeast high-mannose oligosaccharides at resolutions up to 0.95 A. Besides a PA14-like architecture, the Flo5A domain reveals a previously undescribed lectin fold that utilizes a unique DcisD calcium-binding motif for carbohydrate binding and that is widely spread among pro- and eukaryotes. Given the high abundance of high-mannose oligosaccharides in yeast cell walls, the Flo5A structure suggests a model for recognition, where social non-self- instead of unsocial self-interactions are favored.
-
-	Structural basis of flocculin-mediated social behavior in yeast.,Veelders M, Bruckner S, Ott D, Unverzagt C, Mosch HU, Essen LO Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22511-6. Epub 2010 Dec 13. PMID:21149680<ref>PMID:21149680</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2xjt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Flocculation protein 3D structures\|Flocculation protein 3D structures]]		*[[Flocculation protein 3D structures\|Flocculation protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:46, 17 January 2024

OCA — Wed, 17 Jan 2024 12:46:05 GMT

←Older revision		Revision as of 12:46, 17 January 2024
Line 3:		Line 3:
	<StructureSection load='2xjt' size='340' side='right'caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='2xjt' size='340' side='right'caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL~~</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA~~</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.2Å</td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [https://pdbe.org/2xjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [https://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [https://pdbe.org/2xjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [https://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FLO5_YEAST FLO5_YEAST]] Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.	+	[https://www.uniprot.org/uniprot/FLO5_YEAST FLO5_YEAST] Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 2xjt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2xjt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Flocculation protein 3D structures\|Flocculation protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brueckner, S]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Essen, L O]]	+	[[Category: Brueckner S]]
-	[[Category: Moesch, H U]]	+	[[Category: Essen L-O]]
-	[[Category: Ott, D]]	+	[[Category: Moesch H-U]]
-	[[Category: Unverzagt, C]]	+	[[Category: Ott D]]
-	[[Category: Veelders, M]]	+	[[Category: Unverzagt C]]
-	~~[[Category: Carbohydrate binding]]~~	+	[[Category: Veelders M]]
-	~~[[Category: Cell adhesion]]~~	+
-	~~[[Category: Greenbeard]]~~	+
-	~~[[Category: Pa14-domain]]~~	+
-	~~[[Category: Social interaction~~]]	+

OCA at 10:54, 13 April 2022

OCA — Wed, 13 Apr 2022 10:54:29 GMT

←Older revision		Revision as of 10:54, 13 April 2022
Line 3:		Line 3:
	<StructureSection load='2xjt' size='340' side='right'caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='2xjt' size='340' side='right'caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA-D-MANNOSE~~'>~~MAN~~</scene>, <scene name='pdbligand=NA:~~SODIUM+ION~~'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [~~http~~://pdbe.org/2xjt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [https://pdbe.org/2xjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [https://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FLO5_YEAST FLO5_YEAST]] Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.	+	[[https://www.uniprot.org/uniprot/FLO5_YEAST FLO5_YEAST]] Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 15:16, 8 July 2020

OCA — Wed, 08 Jul 2020 15:16:29 GMT

←Older revision		Revision as of 15:16, 8 July 2020
Line 1:		Line 1:

	==X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and Man5(D1)==		==X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and Man5(D1)==
-	<StructureSection load='2xjt' size='340' side='right' caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>	+	<StructureSection load='2xjt' size='340' side='right'caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://pdbe.org/2xjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://pdbe.org/2xjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Brueckner, S]]		[[Category: Brueckner, S]]
	[[Category: Essen, L O]]		[[Category: Essen, L O]]

OCA at 09:18, 3 October 2018

OCA — Wed, 03 Oct 2018 09:18:36 GMT

←Older revision		Revision as of 09:18, 3 October 2018
Line 1:		Line 1:

-	==X-~~RAY STRUCTURE OF THE~~ N-~~TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MAN5~~(D1)==	+	==X-ray structure of the N-terminal domain of the flocculin Flo5 from Saccharomyces cerevisiae in complex with calcium and Man5(D1)==
	<StructureSection load='2xjt' size='340' side='right' caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='2xjt' size='340' side='right' caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:~~SODIUM+ION~~'>NA</scene>, <scene name='pdbligand=~~MAN~~:~~ALPHA-D-MANNOSE~~'>~~MAN~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://pdbe.org/2xjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://pdbe.org/2xjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>

OCA at 10:55, 5 August 2016

OCA — Fri, 05 Aug 2016 10:55:17 GMT

←Older revision		Revision as of 10:55, 5 August 2016
Line 1:		Line 1:
		+
	==X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MAN5(D1)==		==X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MAN5(D1)==
	<StructureSection load='2xjt' size='340' side='right' caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='2xjt' size='340' side='right' caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://pdbe.org/2xjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xjt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xjt" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Baker's yeast]]
	[[Category: Brueckner, S]]		[[Category: Brueckner, S]]
	[[Category: Essen, L O]]		[[Category: Essen, L O]]

OCA at 18:12, 25 December 2014

OCA — Thu, 25 Dec 2014 18:12:48 GMT

←Older revision		Revision as of 18:12, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FLO5_YEAST FLO5_YEAST]] Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca(2+)-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells. Activity is inhibited by mannose, but not by glucose, maltose, sucrose or galactose.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 08:49, 26 November 2014

OCA — Wed, 26 Nov 2014 08:49:51 GMT

←Older revision		Revision as of 08:49, 26 November 2014
Line 1:		Line 1:
-	[[~~Image:~~2xjt.~~png~~\|~~left~~\|~~200px~~]]	+	==X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MAN5(D1)==
		+	<StructureSection load='2xjt' size='340' side='right' caption='[[2xjt]], [[Resolution\|resolution]] 1.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xjt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xjr\|2xjr]], [[2xju\|2xju]], [[2xjv\|2xjv]], [[2xjq\|2xjq]], [[2xjs\|2xjs]], [[2xjp\|2xjp]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjt RCSB], [http://www.ebi.ac.uk/pdbsum/2xjt PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the budding yeast Saccharomyces cerevisiae, self-recognition and the thereby promoted aggregation of thousands of cells into protective flocs is mediated by a family of cell-surface adhesins, the flocculins (Flo). Based on this social behavior FLO genes fulfill the definition of "greenbeard" genes, which direct cooperation toward other carriers of the same gene. The process of flocculation plays an eminent role in the food industry for the production of beer and wine. However, the precise mode of flocculin-mediated surface recognition and the exact structure of cognate ligands have remained elusive. Here, we present structures of the adhesion domain of a flocculin complexed to its cognate ligands derived from yeast high-mannose oligosaccharides at resolutions up to 0.95 A. Besides a PA14-like architecture, the Flo5A domain reveals a previously undescribed lectin fold that utilizes a unique DcisD calcium-binding motif for carbohydrate binding and that is widely spread among pro- and eukaryotes. Given the high abundance of high-mannose oligosaccharides in yeast cell walls, the Flo5A structure suggests a model for recognition, where social non-self- instead of unsocial self-interactions are favored.

-	~~{{STRUCTURE_2xjt\| PDB=2xjt \| SCENE= }}~~	+	Structural basis of flocculin-mediated social behavior in yeast.,Veelders M, Bruckner S, Ott D, Unverzagt C, Mosch HU, Essen LO Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22511-6. Epub 2010 Dec 13. PMID:21149680<ref>PMID:21149680</ref>

-	~~===X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MAN5(D1)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21149680}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2xjt]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XJT OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021149680<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Brueckner, S.]]	+	[[Category: Brueckner, S]]
-	[[Category: Essen, L O.]]	+	[[Category: Essen, L O]]
-	[[Category: Moesch, H U.]]	+	[[Category: Moesch, H U]]
-	[[Category: Ott, D.]]	+	[[Category: Ott, D]]
-	[[Category: Unverzagt, C.]]	+	[[Category: Unverzagt, C]]
-	[[Category: Veelders, M.]]	+	[[Category: Veelders, M]]
	[[Category: Carbohydrate binding]]		[[Category: Carbohydrate binding]]
	[[Category: Cell adhesion]]		[[Category: Cell adhesion]]

OCA at 14:37, 20 February 2013

OCA — Wed, 20 Feb 2013 14:37:40 GMT

←Older revision		Revision as of 14:37, 20 February 2013
Line 1:		Line 1:
	[[Image:2xjt.png\|left\|200px]]		[[Image:2xjt.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_2xjt\| PDB=2xjt \| SCENE= }}		{{STRUCTURE_2xjt\| PDB=2xjt \| SCENE= }}

	===X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MAN5(D1)===		===X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF THE FLOCCULIN FLO5 FROM SACCHAROMYCES CEREVISIAE IN COMPLEX WITH CALCIUM AND MAN5(D1)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21149680}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21149680 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_21149680}}		{{ABSTRACT_PUBMED_21149680}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21149680~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021149680</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Brueckner, S.]]		[[Category: Brueckner, S.]]
Line 30:		Line 19:
	[[Category: Unverzagt, C.]]		[[Category: Unverzagt, C.]]
	[[Category: Veelders, M.]]		[[Category: Veelders, M.]]
		+	[[Category: Carbohydrate binding]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Greenbeard]]
		+	[[Category: Pa14-domain]]
		+	[[Category: Social interaction]]

OCA: Protected "2xjt" [edit=sysop:move=sysop]

OCA — Wed, 13 Apr 2011 04:30:31 GMT

Protected "2xjt" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:30, 13 April 2011