2xkg - Revision history

OCA at 10:40, 16 August 2023

OCA — Wed, 16 Aug 2023 10:40:05 GMT

←Older revision		Revision as of 10:40, 16 August 2023
Line 3:		Line 3:
	<StructureSection load='2xkg' size='340' side='right'caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2xkg' size='340' side='right'caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cerla Cerla~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XKG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XKG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vyz\|2vyz]], [[1v07\|1v07]], [[2vyy\|2vyy]], [[1kr7\|1kr7]], [[2xkh\|2xkh]], [[2xki\|2xki]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [https://pdbe.org/2xkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [https://www.ebi.ac.uk/pdbsum/2xkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [https://pdbe.org/2xkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [https://www.ebi.ac.uk/pdbsum/2xkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GLBN_CERLA GLBN_CERLA]] Acts as an oxygen store capable of sustaining neuronal activity in an anoxic environment for 5 to 30 min.	+	[https://www.uniprot.org/uniprot/GLBN_CERLA GLBN_CERLA] Acts as an oxygen store capable of sustaining neuronal activity in an anoxic environment for 5 to 30 min.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cerla~~]]	+	[[Category: Cerebratulus lacteus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ascenzi, P]]	+	[[Category: Ascenzi P]]
-	[[Category: Blouin~~, G C~~]]	+	[[Category: Blouin GC]]
-	[[Category: Bolognesi, M]]	+	[[Category: Bolognesi M]]
-	[[Category: Capece, L]]	+	[[Category: Capece L]]
-	[[Category: Congia, S]]	+	[[Category: Congia S]]
-	[[Category: Dewilde, S]]	+	[[Category: Dewilde S]]
-	[[Category: Estrin~~, D A~~]]	+	[[Category: Estrin DA]]
-	[[Category: Marti~~, M A~~]]	+	[[Category: Marti MA]]
-	[[Category: Moens, L]]	+	[[Category: Moens L]]
-	[[Category: Nardini, M]]	+	[[Category: Nardini M]]
-	[[Category: Olson~~, J S~~]]	+	[[Category: Olson JS]]
-	[[Category: Pesce, A]]	+	[[Category: Pesce A]]
-	[[Category: Salter~~, M D]]~~	+	[[Category: Salter MD]]
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Oxygen storage~~]]	+

OCA at 08:41, 10 November 2021

OCA — Wed, 10 Nov 2021 08:41:24 GMT

←Older revision		Revision as of 08:41, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='2xkg' size='340' side='right'caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2xkg' size='340' side='right'caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cerla Cerla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XKG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerla Cerla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XKG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vyz\|2vyz]], [[1v07\|1v07]], [[2vyy\|2vyy]], [[1kr7\|1kr7]], [[2xkh\|2xkh]], [[2xki\|2xki]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vyz\|2vyz]], [[1v07\|1v07]], [[2vyy\|2vyy]], [[1kr7\|1kr7]], [[2xkh\|2xkh]], [[2xki\|2xki]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [~~http~~://pdbe.org/2xkg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xkg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [https://pdbe.org/2xkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [https://www.ebi.ac.uk/pdbsum/2xkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GLBN_CERLA GLBN_CERLA]] Acts as an oxygen store capable of sustaining neuronal activity in an anoxic environment for 5 to 30 min.	+	[[https://www.uniprot.org/uniprot/GLBN_CERLA GLBN_CERLA]] Acts as an oxygen store capable of sustaining neuronal activity in an anoxic environment for 5 to 30 min.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:51, 21 May 2019

OCA — Tue, 21 May 2019 07:51:15 GMT

←Older revision		Revision as of 07:51, 21 May 2019
Line 1:		Line 1:

	==C.lacteus mini-Hb Leu86Ala mutant==		==C.lacteus mini-Hb Leu86Ala mutant==
-	<StructureSection load='2xkg' size='340' side='right' caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='2xkg' size='340' side='right'caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerla Cerla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XKG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerla Cerla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XKG FirstGlance]. <br>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Cerla]]		[[Category: Cerla]]
		+	[[Category: Large Structures]]
	[[Category: Ascenzi, P]]		[[Category: Ascenzi, P]]
	[[Category: Blouin, G C]]		[[Category: Blouin, G C]]

OCA at 09:31, 3 October 2018

OCA — Wed, 03 Oct 2018 09:31:29 GMT

←Older revision		Revision as of 09:31, 3 October 2018
Line 1:		Line 1:

-	==C.~~LACTEUS MINI~~-~~HB LEU86ALA MUTANT~~==	+	==C.lacteus mini-Hb Leu86Ala mutant==
	<StructureSection load='2xkg' size='340' side='right' caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2xkg' size='340' side='right' caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 11:11, 5 August 2016

OCA — Fri, 05 Aug 2016 11:11:49 GMT

←Older revision		Revision as of 11:11, 5 August 2016
Line 1:		Line 1:
		+
	==C.LACTEUS MINI-HB LEU86ALA MUTANT==		==C.LACTEUS MINI-HB LEU86ALA MUTANT==
	<StructureSection load='2xkg' size='340' side='right' caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='2xkg' size='340' side='right' caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Cerebratulus_lacteus Cerebratulus lacteus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XKG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerla Cerla]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XKG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vyz\|2vyz]], [[1v07\|1v07]], [[2vyy\|2vyy]], [[1kr7\|1kr7]], [[2xkh\|2xkh]], [[2xki\|2xki]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vyz\|2vyz]], [[1v07\|1v07]], [[2vyy\|2vyy]], [[1kr7\|1kr7]], [[2xkh\|2xkh]], [[2xki\|2xki]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [http://www.ebi.ac.uk/pdbsum/2xkg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [http://pdbe.org/2xkg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [http://www.ebi.ac.uk/pdbsum/2xkg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xkg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cerebratulus lacteus~~]]	+	[[Category: Cerla]]
	[[Category: Ascenzi, P]]		[[Category: Ascenzi, P]]
	[[Category: Blouin, G C]]		[[Category: Blouin, G C]]

OCA at 18:20, 25 December 2014

OCA — Thu, 25 Dec 2014 18:20:10 GMT

←Older revision		Revision as of 18:20, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [http://www.ebi.ac.uk/pdbsum/2xkg PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [http://www.ebi.ac.uk/pdbsum/2xkg PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLBN_CERLA GLBN_CERLA]] Acts as an oxygen store capable of sustaining neuronal activity in an anoxic environment for 5 to 30 min.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Cerebratulus lacteus]]		[[Category: Cerebratulus lacteus]]
-	[[Category: Ascenzi, P.]]	+	[[Category: Ascenzi, P]]
-	[[Category: Blouin, G C.]]	+	[[Category: Blouin, G C]]
-	[[Category: Bolognesi, M.]]	+	[[Category: Bolognesi, M]]
-	[[Category: Capece, L.]]	+	[[Category: Capece, L]]
-	[[Category: Congia, S.]]	+	[[Category: Congia, S]]
-	[[Category: Dewilde, S.]]	+	[[Category: Dewilde, S]]
-	[[Category: Estrin, D A.]]	+	[[Category: Estrin, D A]]
-	[[Category: Marti, M A.]]	+	[[Category: Marti, M A]]
-	[[Category: Moens, L.]]	+	[[Category: Moens, L]]
-	[[Category: Nardini, M.]]	+	[[Category: Nardini, M]]
-	[[Category: Olson, J S.]]	+	[[Category: Olson, J S]]
-	[[Category: Pesce, A.]]	+	[[Category: Pesce, A]]
-	[[Category: Salter, M D.]]	+	[[Category: Salter, M D]]
	[[Category: Metal-binding]]		[[Category: Metal-binding]]
	[[Category: Oxygen storage]]		[[Category: Oxygen storage]]

OCA at 12:51, 22 October 2014

OCA — Wed, 22 Oct 2014 12:51:34 GMT

←Older revision		Revision as of 12:51, 22 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2xkg.~~png~~\|~~left~~\|~~200px~~]]	+	==C.LACTEUS MINI-HB LEU86ALA MUTANT==
		+	<StructureSection load='2xkg' size='340' side='right' caption='[[2xkg]], [[Resolution\|resolution]] 1.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xkg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XKG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vyz\|2vyz]], [[1v07\|1v07]], [[2vyy\|2vyy]], [[1kr7\|1kr7]], [[2xkh\|2xkh]], [[2xki\|2xki]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xkg RCSB], [http://www.ebi.ac.uk/pdbsum/2xkg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The large apolar tunnel traversing the mini-hemoglobin from Cerebratulus lacteus (CerHb) has been examined by X-ray crystallography, ligand binding kinetics, and molecular dynamic simulations. The addition of 10 atm of Xe causes loss of diffraction in wild-type (wt) CerHbO2 crystals, but Leu86(G12)Ala CerHbO2, which has an increased tunnel volume, stably accommodates two discrete Xe atoms: one adjacent to Leu86(G12) and another near Ala55(E18). Molecular dynamics simulations of ligand migration in wt CerHb show a low energy pathway through the apolar tunnel when Leu or Ala, but not Phe or Trp, is present at the 86(G12) position. Addition of 10-15 atm of Xe to solutions of wt CerHbCO and L86A CerHbCO causes 2 to 3-fold increases in the fraction of geminate ligand recombination indicating that the bound Xe blocks CO escape. This idea was confirmed by L86F and L86W mutations, which cause even larger increases in the fraction of geminate CO rebinding, 2 to 5-fold decreases in the bimolecular rate constants for ligand entry, and large increases in the computed energy barriers for ligand movement through the apolar tunnel. Both the addition of Xe to the L86A mutant and oxidation of wt CerHb heme iron cause the appearance of an out Gln44(E7) conformer, in which the amide side chain points out toward the solvent and appears to lower the barrier for ligand escape through the E7 gate. However, the observed kinetics suggest little entry and escape (</= 25%) through the E7 pathway, presumably because the in Gln44(E7) conformer is thermodynamically favored.

-	~~{{STRUCTURE_2xkg\| PDB=2xkg \| SCENE= }}~~	+	Ligand migration in the apolar tunnel of cerebratulus lacteus mini-hemoglobin.,Pesce A, Nardini M, Dewilde S, Capece L, Marti MA, Congia S, Salter MD, Blouin GC, Estrin DA, Ascenzi P, Moens L, Bolognesi M, Olson JS J Biol Chem. 2010 Dec 7. PMID:21147768<ref>PMID:21147768</ref>

-	~~===C.LACTEUS MINI-HB LEU86ALA MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21147768}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2xkg]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA].~~	+

	==See Also==		==See Also==
-	*[[Hemoglobin\|Hemoglobin]]	+	*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021147768<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Cerebratulus lacteus]]		[[Category: Cerebratulus lacteus]]
	[[Category: Ascenzi, P.]]		[[Category: Ascenzi, P.]]

OCA at 19:54, 20 October 2012

OCA — Sat, 20 Oct 2012 19:54:16 GMT

←Older revision		Revision as of 19:54, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2xkg]] is a 1 chain structure ~~of [[Hemoglobin]]~~ with sequence from [http://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA].	+	[[2xkg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA].

	==See Also==		==See Also==

OCA at 08:50, 27 July 2012

OCA — Fri, 27 Jul 2012 08:50:39 GMT

←Older revision		Revision as of 08:50, 27 July 2012
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	[[Image:2xkg.png\|left\|200px]]		[[Image:2xkg.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xkg", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xkg\| PDB=2xkg \| SCENE= }}		{{STRUCTURE_2xkg\| PDB=2xkg \| SCENE= }}

	===C.LACTEUS MINI-HB LEU86ALA MUTANT===		===C.LACTEUS MINI-HB LEU86ALA MUTANT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21147768}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21147768 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_21147768}}		{{ABSTRACT_PUBMED_21147768}}

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	==See Also==		==See Also==
-	*[[Hemoglobin]]	+	*[[Hemoglobin\|Hemoglobin]]

	==Reference==		==Reference==

OCA at 07:58, 13 July 2011

OCA — Wed, 13 Jul 2011 07:58:43 GMT

←Older revision		Revision as of 07:58, 13 July 2011
Line 19:		Line 19:

	==About this Structure==		==About this Structure==
-	[[2xkg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA].	+	[[2xkg]] is a 1 chain structure of [[Hemoglobin]] with sequence from [http://en.wikipedia.org/wiki/Cerebratulus_lacteus Cerebratulus lacteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKG OCA].
		+
		+	==See Also==
		+	*[[Hemoglobin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21147768~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021147768</ref><references group="xtra"/>
	[[Category: Cerebratulus lacteus]]		[[Category: Cerebratulus lacteus]]
	[[Category: Ascenzi, P.]]		[[Category: Ascenzi, P.]]