2xn1 - Revision history

OCA at 08:01, 23 August 2023

OCA — Wed, 23 Aug 2023 08:01:31 GMT

←Older revision		Revision as of 08:01, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='2xn1' size='340' side='right'caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2xn1' size='340' side='right'caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lacac Lacac~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactobacillus_acidophilus_NCFM Lactobacillus acidophilus NCFM]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=TRS:~~2~~-AMINO-2-HYDROXYMETHYL-PROPANE-1,~~3~~-DIOL'>TRS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2xn0\|2xn0]], [[2xn2\|2xn2]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha~~-~~galactosidase Alpha~~-~~galactosidase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.2.~~1~~.22~~ 3~~.2.1.22]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xn1 OCA], [https://pdbe.org/2xn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xn1 RCSB], [https://www.ebi.ac.uk/pdbsum/2xn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xn1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xn1 OCA], [https://pdbe.org/2xn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xn1 RCSB], [https://www.ebi.ac.uk/pdbsum/2xn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xn1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MELA_LACAC MELA_LACAC] Hydrolyzes the short-chain alpha-galactosaccharide raffinose.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alpha-galactosidase]]~~	+	[[Category: Lactobacillus acidophilus NCFM]]
-	~~[[Category: Lacac~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Fredslund, F~~]]	+	[[Category: Abou Hachem M]]
-	[[Category: ~~Hachem, M Abou~~]]	+	[[Category: Fredslund F]]
-	[[Category: Larsen~~, R J~~]]	+	[[Category: Larsen RJ]]
-	[[Category: Leggio, L Lo]]	+	[[Category: Lo Leggio L]]
-	[[Category: Sorensen~~, P G~~]]	+	[[Category: Sorensen PG]]
-	[[Category: Svensson, B]]	+	[[Category: Svensson B]]
-	~~[[Category: Glycosidase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 14:51, 17 November 2021

OCA — Wed, 17 Nov 2021 14:51:30 GMT

←Older revision		Revision as of 14:51, 17 November 2021
Line 3:		Line 3:
	<StructureSection load='2xn1' size='340' side='right'caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2xn1' size='340' side='right'caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lacac Lacac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacac Lacac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xn0\|2xn0]], [[2xn2\|2xn2]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xn0\|2xn0]], [[2xn2\|2xn2]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xn1 OCA], [~~http~~://pdbe.org/2xn1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xn1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xn1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xn1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xn1 OCA], [https://pdbe.org/2xn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xn1 RCSB], [https://www.ebi.ac.uk/pdbsum/2xn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xn1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:55, 25 December 2019

OCA — Wed, 25 Dec 2019 13:55:44 GMT

←Older revision		Revision as of 13:55, 25 December 2019
Line 1:		Line 1:

	==Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS==		==Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS==
-	<StructureSection load='2xn1' size='340' side='right' caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='2xn1' size='340' side='right'caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacac Lacac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacac Lacac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 2xn1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2xn1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galactosidase 3D structures\|Galactosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
	[[Category: Lacac]]		[[Category: Lacac]]
		+	[[Category: Large Structures]]
	[[Category: Fredslund, F]]		[[Category: Fredslund, F]]
	[[Category: Hachem, M Abou]]		[[Category: Hachem, M Abou]]

OCA at 07:32, 17 January 2018

OCA — Wed, 17 Jan 2018 07:32:15 GMT

←Older revision		Revision as of 07:32, 17 January 2018
Line 1:		Line 1:

-	==~~STRUCTURE OF ALPHA~~-~~GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS~~ NCFM ~~WITH~~ TRIS==	+	==Structure of alpha-galactosidase from Lactobacillus acidophilus NCFM with TRIS==
	<StructureSection load='2xn1' size='340' side='right' caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2xn1' size='340' side='right' caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 2xn1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2xn1" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Galactosidase\|Galactosidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:07, 11 August 2016

OCA — Thu, 11 Aug 2016 18:07:43 GMT

←Older revision		Revision as of 18:07, 11 August 2016
Line 1:		Line 1:
		+
	==STRUCTURE OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS NCFM WITH TRIS==		==STRUCTURE OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS NCFM WITH TRIS==
	<StructureSection load='2xn1' size='340' side='right' caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2xn1' size='340' side='right' caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacac Lacac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xn0\|2xn0]], [[2xn2\|2xn2]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xn0\|2xn0]], [[2xn2\|2xn2]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xn1 RCSB], [http://www.ebi.ac.uk/pdbsum/2xn1 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xn1 OCA], [http://pdbe.org/2xn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xn1 RCSB], [http://www.ebi.ac.uk/pdbsum/2xn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xn1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xn1" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
-	[[Category: ~~Lactobacillus acidophilus ncfm~~]]	+	[[Category: Lacac]]
	[[Category: Fredslund, F]]		[[Category: Fredslund, F]]
	[[Category: Hachem, M Abou]]		[[Category: Hachem, M Abou]]

OCA at 07:52, 25 January 2015

OCA — Sun, 25 Jan 2015 07:52:24 GMT

←Older revision		Revision as of 07:52, 25 January 2015
Line 25:		Line 25:
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
	[[Category: Lactobacillus acidophilus ncfm]]		[[Category: Lactobacillus acidophilus ncfm]]
-	[[Category: Fredslund, F.]]	+	[[Category: Fredslund, F]]
-	[[Category: Hachem, M Abou.]]	+	[[Category: Hachem, M Abou]]
-	[[Category: Larsen, R J.]]	+	[[Category: Larsen, R J]]
-	[[Category: Leggio, L Lo.]]	+	[[Category: Leggio, L Lo]]
-	[[Category: Sorensen, P G.]]	+	[[Category: Sorensen, P G]]
-	[[Category: Svensson, B.]]	+	[[Category: Svensson, B]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 12:51, 22 October 2014

OCA — Wed, 22 Oct 2014 12:51:51 GMT

←Older revision		Revision as of 12:51, 22 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2xn1.~~png~~\|~~left~~\|~~200px~~]]	+	==STRUCTURE OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS NCFM WITH TRIS==
		+	<StructureSection load='2xn1' size='340' side='right' caption='[[2xn1]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XN1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xn0\|2xn0]], [[2xn2\|2xn2]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-galactosidase Alpha-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.22 3.2.1.22] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xn1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xn1 RCSB], [http://www.ebi.ac.uk/pdbsum/2xn1 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lactobacillus acidophilus NCFM is a probiotic bacterium known for its beneficial effects on human health. The importance of alpha-galactosidases (alpha-Gals) for growth of probiotic organisms on oligosaccharides of the raffinose family present in many foods is increasingly recognized. Here, the crystal structure of alpha-Gal from L. acidophilus NCFM (LaMel36A) of glycoside hydrolase (GH) family 36 (GH36) is determined by single-wavelength anomalous dispersion. In addition, a 1.58-A-resolution crystallographic complex with alpha-d-galactose at substrate binding subsite -1 was determined. LaMel36A has a large N-terminal twisted beta-sandwich domain, connected by a long alpha-helix to the catalytic (beta/alpha)(8)-barrel domain, and a C-terminal beta-sheet domain. Four identical monomers form a tightly packed tetramer where three monomers contribute to the structural integrity of the active site in each monomer. Structural comparison of LaMel36A with the monomeric Thermotoga maritima alpha-Gal (TmGal36A) reveals that O2 of alpha-d-galactose in LaMel36A interacts with a backbone nitrogen in a glycine-rich loop of the catalytic domain, whereas the corresponding atom in TmGal36A is from a tryptophan side chain belonging to the N-terminal domain. Thus, two distinctly different structural motifs participate in substrate recognition. The tetrameric LaMel36A furthermore has a much deeper active site than the monomeric TmGal36A, which possibly modulates substrate specificity. Sequence analysis of GH36, inspired by the observed structural differences, results in four distinct subgroups having clearly different active-site sequence motifs. This novel subdivision incorporates functional and architectural features and may aid further biochemical and structural analyses within GH36.

-	~~{{STRUCTURE_2xn1\| PDB=2xn1 \| SCENE= }}~~	+	Crystal Structure of alpha-Galactosidase from Lactobacillus acidophilus NCFM: Insight into Tetramer Formation and Substrate Binding.,Fredslund F, Abou Hachem M, Jonsgaard Larsen R, Gerd Sorensen P, Coutinho PM, Lo Leggio L, Svensson B J Mol Biol. 2011 Jul 30. PMID:21827767<ref>PMID:21827767</ref>

-	~~===STRUCTURE OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS NCFM WITH TRIS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21827767}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2xn1]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA].~~	+

	==See Also==		==See Also==
	*[[Galactosidase\|Galactosidase]]		*[[Galactosidase\|Galactosidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021827767<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
	[[Category: Lactobacillus acidophilus ncfm]]		[[Category: Lactobacillus acidophilus ncfm]]

OCA at 12:56, 20 October 2012

OCA — Sat, 20 Oct 2012 12:56:35 GMT

←Older revision		Revision as of 12:56, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2xn1]] is a 4 chain structure ~~of [[Galactosidase]]~~ with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA].	+	[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA].

	==See Also==		==See Also==

OCA at 10:24, 26 July 2012

OCA — Thu, 26 Jul 2012 10:24:27 GMT

←Older revision		Revision as of 10:24, 26 July 2012
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	[[Image:2xn1.png\|left\|200px]]		[[Image:2xn1.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xn1", creates the "Structure Box" on the page.
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	{{STRUCTURE_2xn1\| PDB=2xn1 \| SCENE= }}		{{STRUCTURE_2xn1\| PDB=2xn1 \| SCENE= }}

	===STRUCTURE OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS NCFM WITH TRIS===		===STRUCTURE OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS NCFM WITH TRIS===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21827767}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_21827767}}		{{ABSTRACT_PUBMED_21827767}}

	==About this Structure==		==About this Structure==
-	[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA].	+	[[2xn1]] is a 4 chain structure of [[Galactosidase]] with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA].
		+
		+	==See Also==
		+	*[[Galactosidase\|Galactosidase]]

	==Reference==		==Reference==

OCA at 05:48, 31 August 2011

OCA — Wed, 31 Aug 2011 05:48:10 GMT

←Older revision		Revision as of 05:48, 31 August 2011
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-	[[Image:2xn1.~~jpg~~\|left\|200px]]	+	[[Image:2xn1.png\|left\|200px]]

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	===STRUCTURE OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS NCFM WITH TRIS===		===STRUCTURE OF ALPHA-GALACTOSIDASE FROM LACTOBACILLUS ACIDOPHILUS NCFM WITH TRIS===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21827767}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21827767 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21827767}}

	==About this Structure==		==About this Structure==
	[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA].		[[2xn1]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_acidophilus_ncfm Lactobacillus acidophilus ncfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XN1 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021827767</ref><references group="xtra"/>
	[[Category: Alpha-galactosidase]]		[[Category: Alpha-galactosidase]]
	[[Category: Lactobacillus acidophilus ncfm]]		[[Category: Lactobacillus acidophilus ncfm]]
	[[Category: Fredslund, F.]]		[[Category: Fredslund, F.]]
-	[[Category: Hachem, M A.]]	+	[[Category: Hachem, M Abou.]]
	[[Category: Larsen, R J.]]		[[Category: Larsen, R J.]]
-	[[Category: Leggio, L L.]]	+	[[Category: Leggio, L Lo.]]
	[[Category: Sorensen, P G.]]		[[Category: Sorensen, P G.]]
	[[Category: Svensson, B.]]		[[Category: Svensson, B.]]
	[[Category: Glycosidase]]		[[Category: Glycosidase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]