2xt0 - Revision history

OCA at 10:38, 20 December 2023

OCA — Wed, 20 Dec 2023 10:38:41 GMT

←Older revision		Revision as of 10:38, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2xt0' size='340' side='right'caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2xt0' size='340' side='right'caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aj_13960 Aj 13960~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plesiocystis_pacifica Plesiocystis pacifica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [https://pdbe.org/2xt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [https://www.ebi.ac.uk/pdbsum/2xt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xt0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [https://pdbe.org/2xt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [https://www.ebi.ac.uk/pdbsum/2xt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xt0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A6G7B1_9DELT A6G7B1_9DELT]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aj 13960]]
-	[[Category: Haloalkane dehalogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bogdanovic, X~~]]	+	[[Category: Plesiocystis pacifica]]
-	[[Category: ~~Hinrichs, W~~]]	+	[[Category: Bogdanovic X]]
-	[[Category: ~~Palm, G J~~]]	+	[[Category: Hinrichs W]]
-	[[Category: ~~Alpha-beta hydrolase fold]]~~	+	[[Category: Palm GJ]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 12:16, 27 April 2022

OCA — Wed, 27 Apr 2022 12:16:50 GMT

←Older revision		Revision as of 12:16, 27 April 2022
Line 3:		Line 3:
	<StructureSection load='2xt0' size='340' side='right'caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2xt0' size='340' side='right'caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Aj_13960 Aj 13960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aj_13960 Aj 13960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [~~http~~://pdbe.org/2xt0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xt0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xt0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [https://pdbe.org/2xt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [https://www.ebi.ac.uk/pdbsum/2xt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xt0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:19, 22 July 2020

OCA — Wed, 22 Jul 2020 12:19:58 GMT

←Older revision		Revision as of 12:19, 22 July 2020
Line 1:		Line 1:

	==Dehalogenase DPpA from Plesiocystis pacifica SIR-I==		==Dehalogenase DPpA from Plesiocystis pacifica SIR-I==
-	<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='2xt0' size='340' side='right'caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aj_13960 Aj 13960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aj_13960 Aj 13960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [http://pdbe.org/2xt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xt0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [http://pdbe.org/2xt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xt0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:

	==See Also==		==See Also==
-	*[[Dehalogenase\|Dehalogenase]]	+	*[[Dehalogenase 3D structures\|Dehalogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	[[Category: Aj 13960]]		[[Category: Aj 13960]]
	[[Category: Haloalkane dehalogenase]]		[[Category: Haloalkane dehalogenase]]
		+	[[Category: Large Structures]]
	[[Category: Bogdanovic, X]]		[[Category: Bogdanovic, X]]
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]

OCA at 08:56, 10 October 2018

OCA — Wed, 10 Oct 2018 08:56:08 GMT

←Older revision		Revision as of 08:56, 10 October 2018
Line 1:		Line 1:

-	==~~DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA~~ SIR-I==	+	==Dehalogenase DPpA from Plesiocystis pacifica SIR-I==
	<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 11:56, 5 August 2016

OCA — Fri, 05 Aug 2016 11:56:45 GMT

←Older revision		Revision as of 11:56, 5 August 2016
Line 1:		Line 1:
		+
	==DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA SIR-I==		==DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA SIR-I==
	<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Plesiocystis_pacifica Plesiocystis pacifica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aj_13960 Aj 13960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xt0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [http://pdbe.org/2xt0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xt0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xt0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xt0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dehalogenase\|Dehalogenase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aj 13960]]
	[[Category: Haloalkane dehalogenase]]		[[Category: Haloalkane dehalogenase]]
-	[[Category: Plesiocystis pacifica]]
	[[Category: Bogdanovic, X]]		[[Category: Bogdanovic, X]]
	[[Category: Hinrichs, W]]		[[Category: Hinrichs, W]]

OCA at 22:41, 3 January 2015

OCA — Sat, 03 Jan 2015 22:41:02 GMT

←Older revision		Revision as of 22:41, 3 January 2015
Line 2:		Line 2:
	<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plesiocystis_pacifica Plesiocystis pacifica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. <br>	+	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plesiocystis_pacifica Plesiocystis pacifica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XT0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.~~2 2~~.7.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xt0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xt0 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Cloning, functional expression, biochemical characterization, and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1.,Hesseler M, Bogdanovic X, Hidalgo A, Berenguer J, Palm GJ, Hinrichs W, Bornscheuer UT Appl Microbiol Biotechnol. 2011 Aug;91(4):1049-60. Epub 2011 May 21. PMID:21603934<ref>PMID:21603934</ref>		Cloning, functional expression, biochemical characterization, and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1.,Hesseler M, Bogdanovic X, Hidalgo A, Berenguer J, Palm GJ, Hinrichs W, Bornscheuer UT Appl Microbiol Biotechnol. 2011 Aug;91(4):1049-60. Epub 2011 May 21. PMID:21603934<ref>PMID:21603934</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	[[Category: Haloalkane dehalogenase]]		[[Category: Haloalkane dehalogenase]]
	[[Category: Plesiocystis pacifica]]		[[Category: Plesiocystis pacifica]]
-	[[Category: Bogdanovic, X.]]	+	[[Category: Bogdanovic, X]]
-	[[Category: Hinrichs, W.]]	+	[[Category: Hinrichs, W]]
-	[[Category: Palm, G J.]]	+	[[Category: Palm, G J]]
	[[Category: Alpha-beta hydrolase fold]]		[[Category: Alpha-beta hydrolase fold]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:44, 14 May 2014

OCA — Wed, 14 May 2014 07:44:16 GMT

←Older revision		Revision as of 07:44, 14 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2xt0.~~png~~\|~~left\|200px~~]]	+	==DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA SIR-I==
		+	<StructureSection load='2xt0' size='340' side='right' caption='[[2xt0]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plesiocystis_pacifica Plesiocystis pacifica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xt0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xt0 RCSB], [http://www.ebi.ac.uk/pdbsum/2xt0 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A haloalkane dehalogenase (DppA) from Plesiocystis pacifica SIR-1 was identified by sequence comparison in the NCBI database, cloned, functionally expressed in Escherichia coli, purified, and biochemically characterized. The three-dimensional (3D) structure was determined by X-ray crystallography and has been refined at 1.95 A resolution to an R-factor of 21.93%. The enzyme is composed of an alpha/beta-hydrolase fold and a cap domain and the overall fold is similar to other known haloalkane dehalogenases. Active site residues were identified as Asp123, His278, and Asp249 and Trp124 and Trp163 as halide-stabilizing residues. DppA, like DhlA from Xanthobacter autotrophicus GJ10, is a member of the haloalkane dehalogenase subfamily HLD-I. As a consequence, these enzymes have in common the relative position of their catalytic residues within the structure and also show some similarities in the substrate specificity. The enzyme shows high preference for 1-bromobutane and does not accept chlorinated alkanes, halo acids, or halo alcohols. It is a monomeric protein with a molecular mass of 32.6 kDa and exhibits maximum activity between 33 and 37 degrees C with a pH optimum between pH 8 and 9. The K(m) and k(cat) values for 1-bromobutane were 24.0 mM and 8.08 s(-1). Furthermore, from the 3D-structure of DppA, it was found that the enzyme possesses a large and open active site pocket. Docking experiments were performed to explain the experimentally determined substrate preferences.

-	<!--	+	Cloning, functional expression, biochemical characterization, and structural analysis of a haloalkane dehalogenase from Plesiocystis pacifica SIR-1.,Hesseler M, Bogdanovic X, Hidalgo A, Berenguer J, Palm GJ, Hinrichs W, Bornscheuer UT Appl Microbiol Biotechnol. 2011 Aug;91(4):1049-60. Epub 2011 May 21. PMID:21603934<ref>PMID:21603934</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2xt0"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_2xt0\| PDB=2xt0 \| SCENE= }}~~	+

-	~~===DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA SIR-I===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21603934}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21603934 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21603934}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2xt0]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Plesiocystis_pacifica Plesiocystis pacifica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021603934<~~/~~ref~~><~~ref group="xtra">PMID:020606284</ref><references group="xtra"~~/>	+
	[[Category: Haloalkane dehalogenase]]		[[Category: Haloalkane dehalogenase]]
	[[Category: Plesiocystis pacifica]]		[[Category: Plesiocystis pacifica]]

OCA at 05:09, 21 September 2011

OCA — Wed, 21 Sep 2011 05:09:46 GMT

←Older revision		Revision as of 05:09, 21 September 2011
Line 1:		Line 1:
-	[[Image:2xt0.~~jpg~~\|left\|200px]]	+	[[Image:2xt0.png\|left\|200px]]

	<!--		<!--

OCA at 06:02, 10 August 2011

OCA — Wed, 10 Aug 2011 06:02:35 GMT

←Older revision		Revision as of 06:02, 10 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2xt0.jpg\|left\|200px]]

-	The ~~entry 2xt0~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2xt0", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2xt0\| PDB=2xt0 \| SCENE= }}

-	~~Authors: Bogdanovic, X., Palm, G.J., Hinrichs, W.~~	+	===DEHALOGENASE DPPA FROM PLESIOCYSTIS PACIFICA SIR-I===

-	~~Description~~: ~~Dehalogenase DPpA~~ from Plesiocystis pacifica ~~SIR~~-I	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21603934}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21603934 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21603934}}
		+
		+	==About this Structure==
		+	[[2xt0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plesiocystis_pacifica Plesiocystis pacifica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XT0 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021603934</ref><ref group="xtra">PMID:020606284</ref><references group="xtra"/>
		+	[[Category: Haloalkane dehalogenase]]
		+	[[Category: Plesiocystis pacifica]]
		+	[[Category: Bogdanovic, X.]]
		+	[[Category: Hinrichs, W.]]
		+	[[Category: Palm, G J.]]
		+	[[Category: Alpha-beta hydrolase fold]]
		+	[[Category: Hydrolase]]

OCA: Protected "2xt0" [edit=sysop:move=sysop]

OCA — Wed, 06 Apr 2011 08:24:30 GMT

Protected "2xt0" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:24, 6 April 2011