2xwx - Revision history

OCA at 08:38, 7 February 2024

OCA — Wed, 07 Feb 2024 08:38:19 GMT

←Older revision		Revision as of 08:38, 7 February 2024
Line 3:		Line 3:
	<StructureSection load='2xwx' size='340' side='right'caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2xwx' size='340' side='right'caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XWX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XWX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [https://pdbe.org/2xwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [https://www.ebi.ac.uk/pdbsum/2xwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xwx ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [https://pdbe.org/2xwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [https://www.ebi.ac.uk/pdbsum/2xwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xwx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GBPA_VIBCH GBPA_VIBCH]] Probably interacts with GlcNAc residues. May promote attachment to both epithelial cell surfaces and chitin. This function enhances bacterial colonization in the gastrointestinal tract and may also be important in the environment by augment colonization of chitinous structures, leading to improved survival. Promotes bacterial attachment to, and colonization of, zooplankton in the aquatic ecosystem.<ref>PMID:16341015</ref>	+	[https://www.uniprot.org/uniprot/GBPA_VIBCH GBPA_VIBCH] Probably interacts with GlcNAc residues. May promote attachment to both epithelial cell surfaces and chitin. This function enhances bacterial colonization in the gastrointestinal tract and may also be important in the environment by augment colonization of chitinous structures, leading to improved survival. Promotes bacterial attachment to, and colonization of, zooplankton in the aquatic ecosystem.<ref>PMID:16341015</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Vibrio cholerae is a bacterial pathogen that colonizes the chitinous exoskeleton of zooplankton as well as the human gastrointestinal tract. Colonization of these different niches involves an N-acetylglucosamine binding protein (GbpA) that has been reported to mediate bacterial attachment to both marine chitin and mammalian intestinal mucin through an unknown molecular mechanism. We report structural studies that reveal that GbpA possesses an unusual, elongated, four-domain structure, with domains 1 and 4 showing structural homology to chitin binding domains. A glycan screen revealed that GbpA binds to GlcNAc oligosaccharides. Structure-guided GbpA truncation mutants show that domains 1 and 4 of GbpA interact with chitin in vitro, whereas in vivo complementation studies reveal that domain 1 is also crucial for mucin binding and intestinal colonization. Bacterial binding studies show that domains 2 and 3 bind to the V. cholerae surface. Finally, mouse virulence assays show that only the first three domains of GbpA are required for colonization. These results explain how GbpA provides structural/functional modular interactions between V. cholerae, intestinal epithelium and chitinous exoskeletons.	+
-		+
-	The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces.,Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, Ibrahim AF, Svergun DI, Eijsink VG, Chatterjee NS, van Aalten DM PLoS Pathog. 2012 Jan;8(1):e1002373. Epub 2012 Jan 12. PMID:22253590<ref>PMID:22253590</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 2xwx" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Aalten, D M.F van~~]]	+	[[Category: Vibrio cholerae]]
-	[[Category: Chatterjee~~, N S~~]]	+	[[Category: Chatterjee NS]]
-	[[Category: Eijsink~~, V G.H~~]]	+	[[Category: Eijsink VGH]]
-	[[Category: Ghosh, A]]	+	[[Category: Ghosh A]]
-	[[Category: Guerrero~~, R H~~]]	+	[[Category: Guerrero RH]]
-	[[Category: Ibrahim~~, A F.M~~]]	+	[[Category: Ibrahim AFM]]
-	[[Category: Konarev~~, P V~~]]	+	[[Category: Konarev PV]]
-	[[Category: Svergun~~, D I~~]]	+	[[Category: Svergun DI]]
-	[[Category: Vaaje-Kolstad, G]]	+	[[Category: Vaaje-Kolstad G]]
-	[[Category: Wong, E]]	+	[[Category: Wong E]]
-	[[Category: ~~Chitin-binding protein~~]]	+	[[Category: Van Aalten DMF]]

OCA at 13:21, 4 May 2022

OCA — Wed, 04 May 2022 13:21:47 GMT

←Older revision		Revision as of 13:21, 4 May 2022
Line 3:		Line 3:
	<StructureSection load='2xwx' size='340' side='right'caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2xwx' size='340' side='right'caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2XWX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XWX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [~~http~~://pdbe.org/2xwx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xwx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xwx ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [https://pdbe.org/2xwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [https://www.ebi.ac.uk/pdbsum/2xwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xwx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GBPA_VIBCH GBPA_VIBCH]] Probably interacts with GlcNAc residues. May promote attachment to both epithelial cell surfaces and chitin. This function enhances bacterial colonization in the gastrointestinal tract and may also be important in the environment by augment colonization of chitinous structures, leading to improved survival. Promotes bacterial attachment to, and colonization of, zooplankton in the aquatic ecosystem.<ref>PMID:16341015</ref>	+	[[https://www.uniprot.org/uniprot/GBPA_VIBCH GBPA_VIBCH]] Probably interacts with GlcNAc residues. May promote attachment to both epithelial cell surfaces and chitin. This function enhances bacterial colonization in the gastrointestinal tract and may also be important in the environment by augment colonization of chitinous structures, leading to improved survival. Promotes bacterial attachment to, and colonization of, zooplankton in the aquatic ecosystem.<ref>PMID:16341015</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:53, 29 July 2020

OCA — Wed, 29 Jul 2020 11:53:31 GMT

←Older revision		Revision as of 11:53, 29 July 2020
Line 1:		Line 1:

	==Vibrio cholerae colonization factor GbpA crystal structure==		==Vibrio cholerae colonization factor GbpA crystal structure==
-	<StructureSection load='2xwx' size='340' side='right' caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='2xwx' size='340' side='right'caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XWX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XWX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://pdbe.org/2xwx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xwx ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://pdbe.org/2xwx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xwx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Bacillo virgola del koch trevisan 1884]]		[[Category: Bacillo virgola del koch trevisan 1884]]
		+	[[Category: Large Structures]]
	[[Category: Aalten, D M.F van]]		[[Category: Aalten, D M.F van]]
	[[Category: Chatterjee, N S]]		[[Category: Chatterjee, N S]]

OCA at 17:14, 18 January 2017

OCA — Wed, 18 Jan 2017 17:14:44 GMT

←Older revision		Revision as of 17:14, 18 January 2017
Line 1:		Line 1:
		+
	==Vibrio cholerae colonization factor GbpA crystal structure==		==Vibrio cholerae colonization factor GbpA crystal structure==
	<StructureSection load='2xwx' size='340' side='right' caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2xwx' size='340' side='right' caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XWX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XWX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://pdbe.org/2xwx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xwx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xwx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:17, 15 April 2015

OCA — Wed, 15 Apr 2015 06:17:56 GMT

←Older revision		Revision as of 06:17, 15 April 2015
Line 2:		Line 2:
	<StructureSection load='2xwx' size='340' side='right' caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2xwx' size='340' side='right' caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae Vibrio cholerae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XWX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XWX FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum]</span></td></tr>
	</table>		</table>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio cholerae~~]]	+	[[Category: Bacillo virgola del koch trevisan 1884]]
		+	[[Category: Aalten, D M.F van]]
	[[Category: Chatterjee, N S]]		[[Category: Chatterjee, N S]]
	[[Category: Eijsink, V G.H]]		[[Category: Eijsink, V G.H]]
	[[Category: Ghosh, A]]		[[Category: Ghosh, A]]
	[[Category: Guerrero, R H]]		[[Category: Guerrero, R H]]
		+	[[Category: Ibrahim, A F.M]]
	[[Category: Konarev, P V]]		[[Category: Konarev, P V]]
-	[[Category: Svergun, ~~A F.M Ibrahim~~ D I]]	+	[[Category: Svergun, D I]]
	[[Category: Vaaje-Kolstad, G]]		[[Category: Vaaje-Kolstad, G]]
-	[[Category: Vanaalten, D M.F]]
	[[Category: Wong, E]]		[[Category: Wong, E]]
	[[Category: Chitin-binding protein]]		[[Category: Chitin-binding protein]]

OCA at 13:11, 25 December 2014

OCA — Thu, 25 Dec 2014 13:11:03 GMT

←Older revision		Revision as of 13:11, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XWX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XWX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GBPA_VIBCH GBPA_VIBCH]] Probably interacts with GlcNAc residues. May promote attachment to both epithelial cell surfaces and chitin. This function enhances bacterial colonization in the gastrointestinal tract and may also be important in the environment by augment colonization of chitinous structures, leading to improved survival. Promotes bacterial attachment to, and colonization of, zooplankton in the aquatic ecosystem.<ref>PMID:16341015</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
-	[[Category: Chatterjee, N S.]]	+	[[Category: Chatterjee, N S]]
-	[[Category: Eijsink, V G.H.]]	+	[[Category: Eijsink, V G.H]]
-	[[Category: Ghosh, A.]]	+	[[Category: Ghosh, A]]
-	[[Category: Guerrero, R H.]]	+	[[Category: Guerrero, R H]]
-	[[Category: Konarev, P V.]]	+	[[Category: Konarev, P V]]
-	[[Category: Svergun, A F.M Ibrahim D I.]]	+	[[Category: Svergun, A F.M Ibrahim D I]]
-	[[Category: Vaaje-Kolstad, G.]]	+	[[Category: Vaaje-Kolstad, G]]
-	[[Category: Vanaalten, D M.F.]]	+	[[Category: Vanaalten, D M.F]]
-	[[Category: Wong, E.]]	+	[[Category: Wong, E]]
	[[Category: Chitin-binding protein]]		[[Category: Chitin-binding protein]]

OCA at 06:44, 9 June 2014

OCA — Mon, 09 Jun 2014 06:44:37 GMT

←Older revision		Revision as of 06:44, 9 June 2014
Line 1:		Line 1:
-	[[~~Image~~:2xwx.~~png\|left\|200px~~]]	+	==Vibrio cholerae colonization factor GbpA crystal structure==
		+	<StructureSection load='2xwx' size='340' side='right' caption='[[2xwx]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XWX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xwx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xwx RCSB], [http://www.ebi.ac.uk/pdbsum/2xwx PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vibrio cholerae is a bacterial pathogen that colonizes the chitinous exoskeleton of zooplankton as well as the human gastrointestinal tract. Colonization of these different niches involves an N-acetylglucosamine binding protein (GbpA) that has been reported to mediate bacterial attachment to both marine chitin and mammalian intestinal mucin through an unknown molecular mechanism. We report structural studies that reveal that GbpA possesses an unusual, elongated, four-domain structure, with domains 1 and 4 showing structural homology to chitin binding domains. A glycan screen revealed that GbpA binds to GlcNAc oligosaccharides. Structure-guided GbpA truncation mutants show that domains 1 and 4 of GbpA interact with chitin in vitro, whereas in vivo complementation studies reveal that domain 1 is also crucial for mucin binding and intestinal colonization. Bacterial binding studies show that domains 2 and 3 bind to the V. cholerae surface. Finally, mouse virulence assays show that only the first three domains of GbpA are required for colonization. These results explain how GbpA provides structural/functional modular interactions between V. cholerae, intestinal epithelium and chitinous exoskeletons.

-	~~{{STRUCTURE_2xwx\| PDB=2xwx \| SCENE= }}~~	+	The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces.,Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, Ibrahim AF, Svergun DI, Eijsink VG, Chatterjee NS, van Aalten DM PLoS Pathog. 2012 Jan;8(1):e1002373. Epub 2012 Jan 12. PMID:22253590<ref>PMID:22253590</ref>

-	~~===Vibrio cholerae colonization factor GbpA crystal structure===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22253590}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2xwx]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Vibrio_cholerae Vibrio cholerae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022253590<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Chatterjee, N S.]]		[[Category: Chatterjee, N S.]]

OCA at 05:03, 29 August 2012

OCA — Wed, 29 Aug 2012 05:03:32 GMT

←Older revision		Revision as of 05:03, 29 August 2012
Line 1:		Line 1:
-	[[Image:2xwx.~~jpg~~\|left\|200px]]	+	[[Image:2xwx.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2xwx", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2xwx\| PDB=2xwx \| SCENE= }}		{{STRUCTURE_2xwx\| PDB=2xwx \| SCENE= }}

-	===~~VIBRIO CHOLERAE COLONIZATION FACTOR GBPA CRYSTAL STRUCTURE~~===	+	===Vibrio cholerae colonization factor GbpA crystal structure===

		+	{{ABSTRACT_PUBMED_22253590}}

	==About this Structure==		==About this Structure==
	[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA].		[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022253590</ref><references group="xtra"/>
	[[Category: Vibrio cholerae]]		[[Category: Vibrio cholerae]]
	[[Category: Chatterjee, N S.]]		[[Category: Chatterjee, N S.]]
Line 19:		Line 17:
	[[Category: Ghosh, A.]]		[[Category: Ghosh, A.]]
	[[Category: Guerrero, R H.]]		[[Category: Guerrero, R H.]]
-	[[Category: Ibrahim, A F.M.]]
-	[[Category: Kolstad, G V.]]
	[[Category: Konarev, P V.]]		[[Category: Konarev, P V.]]
-	[[Category: Svergun, D I.]]	+	[[Category: Svergun, A F.M Ibrahim D I.]]
		+	[[Category: Vaaje-Kolstad, G.]]
	[[Category: Vanaalten, D M.F.]]		[[Category: Vanaalten, D M.F.]]
	[[Category: Wong, E.]]		[[Category: Wong, E.]]
	[[Category: Chitin-binding protein]]		[[Category: Chitin-binding protein]]

OCA at 12:24, 16 November 2011

OCA — Wed, 16 Nov 2011 12:24:20 GMT

←Older revision		Revision as of 12:24, 16 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2xwx.jpg\|left\|200px]]

-	The ~~entry 2xwx~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2xwx", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2xwx\| PDB=2xwx \| SCENE= }}

-	~~Authors: Wong, E., Kolstad, G.V., Ghosh, A., Guerrero, R.H., Konarev, P.V., Ibrahim, A.F.M., Svergun, D.I., Eijsink, V.G.H., Chatterjee, N.S., vanAalten, D.M.F.~~	+	===VIBRIO CHOLERAE COLONIZATION FACTOR GBPA CRYSTAL STRUCTURE===

-	~~Description~~: Vibrio cholerae ~~colonization factor GbpA crystal structure~~	+
		+	==About this Structure==
		+	[[2xwx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XWX OCA].
		+	[[Category: Vibrio cholerae]]
		+	[[Category: Chatterjee, N S.]]
		+	[[Category: Eijsink, V G.H.]]
		+	[[Category: Ghosh, A.]]
		+	[[Category: Guerrero, R H.]]
		+	[[Category: Ibrahim, A F.M.]]
		+	[[Category: Kolstad, G V.]]
		+	[[Category: Konarev, P V.]]
		+	[[Category: Svergun, D I.]]
		+	[[Category: Vanaalten, D M.F.]]
		+	[[Category: Wong, E.]]
		+	[[Category: Chitin-binding protein]]

OCA: Protected "2xwx" [edit=sysop:move=sysop]

OCA — Wed, 09 Nov 2011 11:03:45 GMT

Protected "2xwx" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:03, 9 November 2011