2xzc - Revision history

OCA at 18:45, 20 September 2023

OCA — Wed, 20 Sep 2023 18:45:23 GMT

←Older revision		Revision as of 18:45, 20 September 2023
Line 3:		Line 3:
	<StructureSection load='2xzc' size='340' side='right'caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>		<StructureSection load='2xzc' size='340' side='right'caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XZC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XZC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO~~[3.~~2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.36Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~PCA~~:~~PYROGLUTAMIC~~+~~ACID~~'>~~PCA~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO[3.2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max~~-~~height: 3em;'>~~[~~[2xza\|2xza]~~]</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [https://pdbe.org/2xzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [https://www.ebi.ac.uk/pdbsum/2xzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xzc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [https://pdbe.org/2xzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [https://www.ebi.ac.uk/pdbsum/2xzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xzc ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Carletti, E]]	+	[[Category: Carletti E]]
-	[[Category: Friboulet, A]]	+	[[Category: Friboulet A]]
-	[[Category: Gabibov, A]]	+	[[Category: Gabibov A]]
-	[[Category: Kurkova, I]]	+	[[Category: Kurkova I]]
-	[[Category: Masson, P]]	+	[[Category: Masson P]]
-	[[Category: Nachon, F]]	+	[[Category: Nachon F]]
-	[[Category: Nicolet, Y]]	+	[[Category: Nicolet Y]]
-	[[Category: Smirnov, I]]	+	[[Category: Smirnov I]]
-	[[Category: Tramontano, A]]	+	[[Category: Tramontano A]]
-	~~[[Category: Immune system~~]]	+

OCA at 17:29, 15 December 2021

OCA — Wed, 15 Dec 2021 17:29:07 GMT

←Older revision		Revision as of 17:29, 15 December 2021
Line 3:		Line 3:
	<StructureSection load='2xzc' size='340' side='right'caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>		<StructureSection load='2xzc' size='340' side='right'caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2XZC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XZC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO[3.2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO[3.2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xza\|2xza]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2xza\|2xza]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [~~http~~://pdbe.org/2xzc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2xzc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2xzc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [https://pdbe.org/2xzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [https://www.ebi.ac.uk/pdbsum/2xzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xzc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:40, 11 March 2020

OCA — Wed, 11 Mar 2020 07:40:59 GMT

←Older revision		Revision as of 07:40, 11 March 2020
Line 1:		Line 1:

	==Crystal Structure of phosphonate-modified recombinant A.17 antibody FAB fragment==		==Crystal Structure of phosphonate-modified recombinant A.17 antibody FAB fragment==
-	<StructureSection load='2xzc' size='340' side='right' caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>	+	<StructureSection load='2xzc' size='340' side='right'caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XZC FirstGlance]. <br>		<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XZC FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Carletti, E]]		[[Category: Carletti, E]]
	[[Category: Friboulet, A]]		[[Category: Friboulet, A]]

OCA at 09:20, 10 October 2018

OCA — Wed, 10 Oct 2018 09:20:34 GMT

←Older revision		Revision as of 09:20, 10 October 2018
Line 1:		Line 1:

-	==~~CRYSTAL STRUCTURE OF PHOSPHONATE~~-~~MODIFIED RECOMBINANT~~ A.17 ~~ANTIBODY~~ FAB ~~FRAGMENT~~==	+	==Crystal Structure of phosphonate-modified recombinant A.17 antibody FAB fragment==
	<StructureSection load='2xzc' size='340' side='right' caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>		<StructureSection load='2xzc' size='340' side='right' caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 15:00, 5 August 2016

OCA — Fri, 05 Aug 2016 15:00:42 GMT

←Older revision		Revision as of 15:00, 5 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF PHOSPHONATE-MODIFIED RECOMBINANT A.17 ANTIBODY FAB FRAGMENT==		==CRYSTAL STRUCTURE OF PHOSPHONATE-MODIFIED RECOMBINANT A.17 ANTIBODY FAB FRAGMENT==
	<StructureSection load='2xzc' size='340' side='right' caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>		<StructureSection load='2xzc' size='340' side='right' caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XZC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XZC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO[3.2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO[3.2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xza\|2xza]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xza\|2xza]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [http://www.ebi.ac.uk/pdbsum/2xzc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [http://pdbe.org/2xzc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [http://www.ebi.ac.uk/pdbsum/2xzc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xzc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2xzc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Carletti, E]]		[[Category: Carletti, E]]
	[[Category: Friboulet, A]]		[[Category: Friboulet, A]]

OCA at 23:06, 3 January 2015

OCA — Sat, 03 Jan 2015 23:06:08 GMT

←Older revision		Revision as of 23:06, 3 January 2015
Line 2:		Line 2:
	<StructureSection load='2xzc' size='340' side='right' caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>		<StructureSection load='2xzc' size='340' side='right' caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. <br>	+	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XZC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO[3.2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO[3.2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xza\|2xza]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xza\|2xza]]</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [http://www.ebi.ac.uk/pdbsum/2xzc PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [http://www.ebi.ac.uk/pdbsum/2xzc PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 14:
	Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics.,Smirnov I, Carletti E, Kurkova I, Nachon F, Nicolet Y, Mitkevich VA, Debat H, Avalle B, Belogurov AA Jr, Kuznetsov N, Reshetnyak A, Masson P, Tonevitsky AG, Ponomarenko N, Makarov AA, Friboulet A, Tramontano A, Gabibov A Proc Natl Acad Sci U S A. 2011 Sep 6. PMID:21896761<ref>PMID:21896761</ref>		Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics.,Smirnov I, Carletti E, Kurkova I, Nachon F, Nicolet Y, Mitkevich VA, Debat H, Avalle B, Belogurov AA Jr, Kuznetsov N, Reshetnyak A, Masson P, Tonevitsky AG, Ponomarenko N, Makarov AA, Friboulet A, Tramontano A, Gabibov A Proc Natl Acad Sci U S A. 2011 Sep 6. PMID:21896761<ref>PMID:21896761</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Carletti, E.]]	+	[[Category: Carletti, E]]
-	[[Category: Friboulet, A.]]	+	[[Category: Friboulet, A]]
-	[[Category: Gabibov, A.]]	+	[[Category: Gabibov, A]]
-	[[Category: Kurkova, I.]]	+	[[Category: Kurkova, I]]
-	[[Category: Masson, P.]]	+	[[Category: Masson, P]]
-	[[Category: Nachon, F.]]	+	[[Category: Nachon, F]]
-	[[Category: Nicolet, Y.]]	+	[[Category: Nicolet, Y]]
-	[[Category: Smirnov, I.]]	+	[[Category: Smirnov, I]]
-	[[Category: Tramontano, A.]]	+	[[Category: Tramontano, A]]
	[[Category: Immune system]]		[[Category: Immune system]]

OCA at 07:41, 14 May 2014

OCA — Wed, 14 May 2014 07:41:45 GMT

←Older revision		Revision as of 07:41, 14 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2xzc.~~png~~\|~~left~~\|~~200px~~]]	+	==CRYSTAL STRUCTURE OF PHOSPHONATE-MODIFIED RECOMBINANT A.17 ANTIBODY FAB FRAGMENT==
		+	<StructureSection load='2xzc' size='340' side='right' caption='[[2xzc]], [[Resolution\|resolution]] 1.36Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2xzc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=XOP:8-METHYL-8-AZABICYCLO[3.2.1]OCTAN-3-YL+PHENYLPHOSPHONATE'>XOP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2xza\|2xza]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xzc RCSB], [http://www.ebi.ac.uk/pdbsum/2xzc PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Igs offer a versatile template for combinatorial and rational design approaches to the de novo creation of catalytically active proteins. We have used a covalent capture selection strategy to identify biocatalysts from within a human semisynthetic antibody variable fragment library that uses a nucleophilic mechanism. Specific phosphonylation at a single tyrosine within the variable light-chain framework was confirmed in a recombinant IgG construct. High-resolution crystallographic structures of unmodified and phosphonylated Fabs display a 15-A-deep two-chamber cavity at the interface of variable light (V(L)) and variable heavy (V(H)) fragments having a nucleophilic tyrosine at the base of the site. The depth and structure of the pocket are atypical of antibodies in general but can be compared qualitatively with the catalytic site of cholinesterases. A structurally disordered heavy chain complementary determining region 3 loop, constituting a wall of the cleft, is stabilized after covalent modification by hydrogen bonding to the phosphonate tropinol moiety. These features and presteady state kinetics analysis indicate that an induced fit mechanism operates in this reaction. Mutations of residues located in this stabilized loop do not interfere with direct contacts to the organophosphate ligand but can interrogate second shell interactions, because the H3 loop has a conformation adjusted for binding. Kinetic and thermodynamic parameters along with computational docking support the active site model, including plasticity and simple catalytic components. Although relatively uncomplicated, this catalytic machinery displays both stereo- and chemical selectivity. The organophosphate pesticide paraoxon is hydrolyzed by covalent catalysis with rate-limiting dephosphorylation. This reactibody is, therefore, a kinetically selected protein template that has enzyme-like catalytic attributes.

-	~~<!--~~	+	Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics.,Smirnov I, Carletti E, Kurkova I, Nachon F, Nicolet Y, Mitkevich VA, Debat H, Avalle B, Belogurov AA Jr, Kuznetsov N, Reshetnyak A, Masson P, Tonevitsky AG, Ponomarenko N, Makarov AA, Friboulet A, Tramontano A, Gabibov A Proc Natl Acad Sci U S A. 2011 Sep 6. PMID:21896761<ref>PMID:21896761</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2xzc"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2xzc\| PDB=2xzc \| SCENE= }}~~	+

-	~~===CRYSTAL STRUCTURE OF PHOSPHONATE-MODIFIED RECOMBINANT A.17 ANTIBODY FAB FRAGMENT===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21896761}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21896761 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21896761}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2xzc]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021896761<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Carletti, E.]]		[[Category: Carletti, E.]]

OCA at 05:40, 5 October 2011

OCA — Wed, 05 Oct 2011 05:40:43 GMT

←Older revision		Revision as of 05:40, 5 October 2011
Line 1:		Line 1:
-	[[Image:2xzc.~~jpg~~\|left\|200px]]	+	[[Image:2xzc.png\|left\|200px]]

	<!--		<!--

OCA: Protected "2xzc" [edit=sysop:move=sysop]

OCA — Wed, 21 Sep 2011 05:54:32 GMT

Protected "2xzc" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:54, 21 September 2011

OCA at 05:54, 21 September 2011

OCA — Wed, 21 Sep 2011 05:54:27 GMT

←Older revision		Revision as of 05:54, 21 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2xzc.jpg\|left\|200px]]

-	The ~~entry 2xzc~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2xzc", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2xzc\| PDB=2xzc \| SCENE= }}

-	~~Authors: Carletti, E., Nachon, F., Nicolet, Y., Masson, P~~.	+	===CRYSTAL STRUCTURE OF PHOSPHONATE-MODIFIED RECOMBINANT A.17 ANTIBODY FAB FRAGMENT===

-	Description: Crystal Structure of phosphonate-modified recombinant A.17 antibody FAB fragment

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Dec 8 11~~:25:~~14 2010''~~	+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21896761}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21896761 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21896761}}
		+
		+	==About this Structure==
		+	[[2xzc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZC OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021896761</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Carletti, E.]]
		+	[[Category: Friboulet, A.]]
		+	[[Category: Gabibov, A.]]
		+	[[Category: Kurkova, I.]]
		+	[[Category: Masson, P.]]
		+	[[Category: Nachon, F.]]
		+	[[Category: Nicolet, Y.]]
		+	[[Category: Smirnov, I.]]
		+	[[Category: Tramontano, A.]]
		+	[[Category: Immune system]]