2yfr - Revision history

OCA at 10:51, 20 December 2023

OCA — Wed, 20 Dec 2023 10:51:44 GMT

←Older revision		Revision as of 10:51, 20 December 2023
Line 3:		Line 3:
	<StructureSection load='2yfr' size='340' side='right'caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2yfr' size='340' side='right'caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_33200 Atcc 33200~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YFR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YFR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2yfs\|2yfs]], [[2yft\|2yft]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Inulosucrase Inulosucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.9 2.4.1.9] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [https://pdbe.org/2yfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [https://www.ebi.ac.uk/pdbsum/2yfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yfr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [https://pdbe.org/2yfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [https://www.ebi.ac.uk/pdbsum/2yfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yfr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/INUS_LACJO INUS_LACJO] Fructosyltransferase that catalyzes the polymerization of the fructose moiety of sucrose to produce inulin polymer and inulin oligosaccharides such as 1-kestose and nystose.<ref>PMID:18408060</ref> <ref>PMID:21801732</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 33200]]~~	+	[[Category: Lactobacillus johnsonii]]
-	~~[[Category: Inulosucrase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Anwar~~, M A~~]]	+	[[Category: Anwar MA]]
-	[[Category: Dijkhuizen, L]]	+	[[Category: Dijkhuizen L]]
-	[[Category: Dijkstra~~, B W~~]]	+	[[Category: Dijkstra BW]]
-	[[Category: Kralj, S]]	+	[[Category: Kralj S]]
-	[[Category: Leemhuis, H]]	+	[[Category: Leemhuis H]]
-	[[Category: Pijning, T]]	+	[[Category: Pijning T]]
-	~~[[Category: Fructosyltransferase]]~~	+
-	~~[[Category: Glycoside hydrolase family gh68]]~~	+
-	~~[[Category: Sugar utilization]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:28, 27 April 2022

OCA — Wed, 27 Apr 2022 12:28:48 GMT

←Older revision		Revision as of 12:28, 27 April 2022
Line 3:		Line 3:
	<StructureSection load='2yfr' size='340' side='right'caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2yfr' size='340' side='right'caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_33200 Atcc 33200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2YFR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33200 Atcc 33200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YFR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yfs\|2yfs]], [[2yft\|2yft]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2yfs\|2yfs]], [[2yft\|2yft]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Inulosucrase Inulosucrase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.9 2.4.1.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Inulosucrase Inulosucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.9 2.4.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [~~http~~://pdbe.org/2yfr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2yfr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2yfr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [https://pdbe.org/2yfr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [https://www.ebi.ac.uk/pdbsum/2yfr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yfr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:48, 22 July 2020

OCA — Wed, 22 Jul 2020 12:48:35 GMT

←Older revision		Revision as of 12:48, 22 July 2020
Line 1:		Line 1:

	==Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533==		==Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533==
-	<StructureSection load='2yfr' size='340' side='right' caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='2yfr' size='340' side='right'caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33200 Atcc 33200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2YFR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33200 Atcc 33200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2YFR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yfs\|2yfs]], [[2yft\|2yft]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yfs\|2yfs]], [[2yft\|2yft]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inulosucrase Inulosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.9 2.4.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inulosucrase Inulosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.9 2.4.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [http://pdbe.org/2yfr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [http://www.ebi.ac.uk/pdbsum/2yfr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yfr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [http://pdbe.org/2yfr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [http://www.ebi.ac.uk/pdbsum/2yfr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yfr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 24:		Line 24:
	[[Category: Atcc 33200]]		[[Category: Atcc 33200]]
	[[Category: Inulosucrase]]		[[Category: Inulosucrase]]
		+	[[Category: Large Structures]]
	[[Category: Anwar, M A]]		[[Category: Anwar, M A]]
	[[Category: Dijkhuizen, L]]		[[Category: Dijkhuizen, L]]

OCA at 09:06, 10 October 2018

OCA — Wed, 10 Oct 2018 09:06:58 GMT

←Older revision		Revision as of 09:06, 10 October 2018
Line 1:		Line 1:

-	==~~CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII~~ NCC533==	+	==Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533==
	<StructureSection load='2yfr' size='340' side='right' caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2yfr' size='340' side='right' caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 08:21, 5 August 2016

OCA — Fri, 05 Aug 2016 08:21:16 GMT

←Older revision		Revision as of 08:21, 5 August 2016
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII NCC533==		==CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII NCC533==
	<StructureSection load='2yfr' size='340' side='right' caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2yfr' size='340' side='right' caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Lactobacillus_johnsonii Lactobacillus johnsonii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YFR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33200 Atcc 33200]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YFR FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yfs\|2yfs]], [[2yft\|2yft]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yfs\|2yfs]], [[2yft\|2yft]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inulosucrase Inulosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.9 2.4.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inulosucrase Inulosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.9 2.4.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [http://www.ebi.ac.uk/pdbsum/2yfr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [http://pdbe.org/2yfr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [http://www.ebi.ac.uk/pdbsum/2yfr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yfr ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2yfr" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33200]]
	[[Category: Inulosucrase]]		[[Category: Inulosucrase]]
-	[[Category: Lactobacillus johnsonii]]
	[[Category: Anwar, M A]]		[[Category: Anwar, M A]]
	[[Category: Dijkhuizen, L]]		[[Category: Dijkhuizen, L]]

OCA at 21:30, 3 January 2015

OCA — Sat, 03 Jan 2015 21:30:02 GMT

←Older revision		Revision as of 21:30, 3 January 2015
Line 2:		Line 2:
	<StructureSection load='2yfr' size='340' side='right' caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='2yfr' size='340' side='right' caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. <br>	+	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YFR FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yfs\|2yfs]], [[2yft\|2yft]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yfs\|2yfs]], [[2yft\|2yft]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Inulosucrase Inulosucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.9 2.4.1.9] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [http://www.ebi.ac.uk/pdbsum/2yfr PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [http://www.ebi.ac.uk/pdbsum/2yfr PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Crystal Structure of Inulosucrase from Lactobacillus: Insights into the Substrate Specificity and Product Specificity of GH68 Fructansucrases.,Pijning T, Anwar MA, Boger M, Dobruchowska JM, Leemhuis H, Kralj S, Dijkhuizen L, Dijkstra BW J Mol Biol. 2011 Sep 9;412(1):80-93. Epub 2011 Jul 23. PMID:21801732<ref>PMID:21801732</ref>		Crystal Structure of Inulosucrase from Lactobacillus: Insights into the Substrate Specificity and Product Specificity of GH68 Fructansucrases.,Pijning T, Anwar MA, Boger M, Dobruchowska JM, Leemhuis H, Kralj S, Dijkhuizen L, Dijkstra BW J Mol Biol. 2011 Sep 9;412(1):80-93. Epub 2011 Jul 23. PMID:21801732<ref>PMID:21801732</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 22:
	[[Category: Inulosucrase]]		[[Category: Inulosucrase]]
	[[Category: Lactobacillus johnsonii]]		[[Category: Lactobacillus johnsonii]]
-	[[Category: Anwar, M A.]]	+	[[Category: Anwar, M A]]
-	[[Category: Dijkhuizen, L.]]	+	[[Category: Dijkhuizen, L]]
-	[[Category: Dijkstra, B W.]]	+	[[Category: Dijkstra, B W]]
-	[[Category: Kralj, S.]]	+	[[Category: Kralj, S]]
-	[[Category: Leemhuis, H.]]	+	[[Category: Leemhuis, H]]
-	[[Category: Pijning, T.]]	+	[[Category: Pijning, T]]
	[[Category: Fructosyltransferase]]		[[Category: Fructosyltransferase]]
	[[Category: Glycoside hydrolase family gh68]]		[[Category: Glycoside hydrolase family gh68]]
	[[Category: Sugar utilization]]		[[Category: Sugar utilization]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 07:49, 14 May 2014

OCA — Wed, 14 May 2014 07:49:15 GMT

←Older revision		Revision as of 07:49, 14 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2yfr.~~png~~\|~~left~~\|~~200px~~]]	+	==CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII NCC533==
		+	<StructureSection load='2yfr' size='340' side='right' caption='[[2yfr]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2yfs\|2yfs]], [[2yft\|2yft]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yfr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yfr RCSB], [http://www.ebi.ac.uk/pdbsum/2yfr PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fructansucrases (FSs) catalyze a transfructosylation reaction with sucrose as substrate to produce fructo-oligosaccharides and fructan polymers that contain either beta-2,1 glycosidic linkages (inulin) or beta-2,6 linkages (levan). Levan-synthesizing FSs (levansucrases) have been most extensively investigated, while detailed information on inulosucrases is limited. Importantly, the molecular basis of the different product specificities of levansucrases and inulosucrases is poorly understood. We have elucidated the three-dimensional structure of a truncated active bacterial GH68 inulosucrase, InuJ of Lactobacillus johnsonii NCC533 (residues 145-708), in its apo form, with a bound substrate (sucrose), and with a transfructosylation product. The sucrose binding pocket and the sucrose binding mode are virtually identical with those of GH68 levansucrases, confirming that both enzyme types use the same fully conserved structural framework for the binding and cleavage of the donor substrate sucrose in the active site. The binding mode of the first transfructosylation product 1-kestose (Fru-beta(2-1)-Fru-alpha(2-1)-Glc, where Fru=fructose and Glc=glucose) in subsites -1 to +2 shows for the first time how inulin-type fructo-oligosaccharide bind in GH68 FS and how an inulin-type linkage can be formed. Surprisingly, observed interactions with the sugar in subsites +1 and +2 are provided by residues that are also present in levansucrases. The binding mode of 1-kestose and the presence of a more distant sucrose binding site suggest that residues beyond the +2 subsite, in particular residues from the nonconserved 1B-1C loop, determine product linkage type specificity in GH68 FSs.

-	~~<!--~~	+	Crystal Structure of Inulosucrase from Lactobacillus: Insights into the Substrate Specificity and Product Specificity of GH68 Fructansucrases.,Pijning T, Anwar MA, Boger M, Dobruchowska JM, Leemhuis H, Kralj S, Dijkhuizen L, Dijkstra BW J Mol Biol. 2011 Sep 9;412(1):80-93. Epub 2011 Jul 23. PMID:21801732<ref>PMID:21801732</ref>
-	~~The line below this paragraph, containing "STRUCTURE_2yfr", creates the "~~Structure ~~Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_2yfr\| PDB=2yfr \| SCENE= }}~~	+

-	~~===CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII NCC533===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21801732}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21801732 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21801732}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2yfr]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021801732<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Inulosucrase]]		[[Category: Inulosucrase]]
	[[Category: Lactobacillus johnsonii]]		[[Category: Lactobacillus johnsonii]]

OCA at 05:53, 31 August 2011

OCA — Wed, 31 Aug 2011 05:53:32 GMT

←Older revision		Revision as of 05:53, 31 August 2011
Line 1:		Line 1:
-	[[Image:2yfr.~~jpg~~\|left\|200px]]	+	[[Image:2yfr.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII NCC533===		===CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII NCC533===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21801732}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21801732 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21801732}}

	==About this Structure==		==About this Structure==
	[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA].		[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021801732</ref><references group="xtra"/>
	[[Category: Inulosucrase]]		[[Category: Inulosucrase]]
	[[Category: Lactobacillus johnsonii]]		[[Category: Lactobacillus johnsonii]]

OCA at 08:53, 3 August 2011

OCA — Wed, 03 Aug 2011 08:53:39 GMT

←Older revision		Revision as of 08:53, 3 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2yfr.jpg\|left\|200px]]

-	The ~~entry 2yfr~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2yfr", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2yfr\| PDB=2yfr \| SCENE= }}

-	~~Authors: Pijning, T., Anwar, M.A., Leemhuis, H., Kralj, S., Dijkhuizen, L., Dijkstra, B.W.~~	+	===CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII NCC533===

-	~~Description: Crystal~~ structure ~~of inulosucrase~~ from Lactobacillus johnsonii ~~NCC533~~	+
		+	==About this Structure==
		+	[[2yfr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFR OCA].
		+	[[Category: Inulosucrase]]
		+	[[Category: Lactobacillus johnsonii]]
		+	[[Category: Anwar, M A.]]
		+	[[Category: Dijkhuizen, L.]]
		+	[[Category: Dijkstra, B W.]]
		+	[[Category: Kralj, S.]]
		+	[[Category: Leemhuis, H.]]
		+	[[Category: Pijning, T.]]
		+	[[Category: Fructosyltransferase]]
		+	[[Category: Glycoside hydrolase family gh68]]
		+	[[Category: Sugar utilization]]
		+	[[Category: Transferase]]

OCA at 10:09, 21 April 2011

OCA — Thu, 21 Apr 2011 10:09:41 GMT

←Older revision		Revision as of 10:09, 21 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2yfr is ON HOLD	+	The entry 2yfr is ON HOLD until Paper Publication

	Authors: Pijning, T., Anwar, M.A., Leemhuis, H., Kralj, S., Dijkhuizen, L., Dijkstra, B.W.		Authors: Pijning, T., Anwar, M.A., Leemhuis, H., Kralj, S., Dijkhuizen, L., Dijkstra, B.W.

	Description: Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533		Description: Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533