2yin - Revision history

OCA at 08:14, 23 August 2023

OCA — Wed, 23 Aug 2023 08:14:25 GMT

←Older revision		Revision as of 08:14, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='2yin' size='340' side='right'caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2yin' size='340' side='right'caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YIN FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[2vrw\|2vrw]]~~, [[~~1e96~~\|~~1e96~~]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[1ryf\|1ryf]], [[2wkq\|2wkq]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[2wkr\|2wkr]], [[2wkp\|2wkp]], [[2fju\|2fju]], [[1g4u\|1g4u]], [[1i4t\|1i4t]]</div></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [https://pdbe.org/2yin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [https://www.ebi.ac.uk/pdbsum/2yin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yin ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [https://pdbe.org/2yin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [https://www.ebi.ac.uk/pdbsum/2yin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yin ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DOCK2_HUMAN DOCK2_HUMAN]] Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.<ref>PMID:21613211</ref> [[https://www.uniprot.org/uniprot/RAC1_HUMAN RAC1_HUMAN]] Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref>	+	[https://www.uniprot.org/uniprot/DOCK2_HUMAN DOCK2_HUMAN] Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.<ref>PMID:21613211</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barford, D]]	+	[[Category: Barford D]]
-	[[Category: Kulkarni~~, K A~~]]	+	[[Category: Kulkarni KA]]
-	[[Category: Yang, J]]	+	[[Category: Yang J]]
-	[[Category: Zhang, Z]]	+	[[Category: Zhang Z]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Dock]]~~	+
-	~~[[Category: Dock guanine nucleotide exchange factor]]~~	+
-	~~[[Category: Rho gtpase~~]]	+

OCA at 15:05, 17 November 2021

OCA — Wed, 17 Nov 2021 15:05:56 GMT

←Older revision		Revision as of 15:05, 17 November 2021
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	<StructureSection load='2yin' size='340' side='right'caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2yin' size='340' side='right'caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2YIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YIN FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[1ryf\|1ryf]], [[2wkq\|2wkq]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[2wkr\|2wkr]], [[2wkp\|2wkp]], [[2fju\|2fju]], [[1g4u\|1g4u]], [[1i4t\|1i4t]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[1ryf\|1ryf]], [[2wkq\|2wkq]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[2wkr\|2wkr]], [[2wkp\|2wkp]], [[2fju\|2fju]], [[1g4u\|1g4u]], [[1i4t\|1i4t]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [~~http~~://pdbe.org/2yin PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2yin PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2yin ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [https://pdbe.org/2yin PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [https://www.ebi.ac.uk/pdbsum/2yin PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yin ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DOCK2_HUMAN DOCK2_HUMAN]] Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.<ref>PMID:21613211</ref> [[~~http~~://www.uniprot.org/uniprot/RAC1_HUMAN RAC1_HUMAN]] Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref>	+	[[https://www.uniprot.org/uniprot/DOCK2_HUMAN DOCK2_HUMAN]] Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.<ref>PMID:21613211</ref> [[https://www.uniprot.org/uniprot/RAC1_HUMAN RAC1_HUMAN]] Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Dedicator of cytokinesis protein\|Dedicator of cytokinesis protein]]	+	*[[Dedicator of cytokinesis protein 3D structures\|Dedicator of cytokinesis protein 3D structures]]
-	*[[Rac\|Rac]]	+	*[[Rac 3D structures\|Rac 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:28, 10 May 2019

OCA — Fri, 10 May 2019 12:28:39 GMT

←Older revision		Revision as of 12:28, 10 May 2019
Line 1:		Line 1:

	==STRUCTURE OF THE COMPLEX BETWEEN Dock2 AND Rac1.==		==STRUCTURE OF THE COMPLEX BETWEEN Dock2 AND Rac1.==
-	<StructureSection load='2yin' size='340' side='right' caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='2yin' size='340' side='right'caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YIN FirstGlance]. <br>		<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YIN FirstGlance]. <br>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Kulkarni, K A]]		[[Category: Kulkarni, K A]]

OCA at 09:14, 17 October 2018

OCA — Wed, 17 Oct 2018 09:14:53 GMT

←Older revision		Revision as of 09:14, 17 October 2018
Line 1:		Line 1:

-	==STRUCTURE OF THE COMPLEX BETWEEN ~~DOCK2~~ AND ~~RAC1~~.==	+	==STRUCTURE OF THE COMPLEX BETWEEN Dock2 AND Rac1.==
	<StructureSection load='2yin' size='340' side='right' caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2yin' size='340' side='right' caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 2yin" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2yin" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dedicator of cytokinesis protein\|Dedicator of cytokinesis protein]]
		+	*[[Rac\|Rac]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:08, 4 August 2016

OCA — Thu, 04 Aug 2016 15:08:46 GMT

←Older revision		Revision as of 15:08, 4 August 2016
Line 1:		Line 1:
		+
	==STRUCTURE OF THE COMPLEX BETWEEN DOCK2 AND RAC1.==		==STRUCTURE OF THE COMPLEX BETWEEN DOCK2 AND RAC1.==
	<StructureSection load='2yin' size='340' side='right' caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2yin' size='340' side='right' caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YIN FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[1ryf\|1ryf]], [[2wkq\|2wkq]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[2wkr\|2wkr]], [[2wkp\|2wkp]], [[2fju\|2fju]], [[1g4u\|1g4u]], [[1i4t\|1i4t]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[1ryf\|1ryf]], [[2wkq\|2wkq]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[2wkr\|2wkr]], [[2wkp\|2wkp]], [[2fju\|2fju]], [[1g4u\|1g4u]], [[1i4t\|1i4t]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [http://www.ebi.ac.uk/pdbsum/2yin PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [http://pdbe.org/2yin PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [http://www.ebi.ac.uk/pdbsum/2yin PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yin ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/RAC1_HUMAN RAC1_HUMAN]] Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref>	+	[[http://www.uniprot.org/uniprot/DOCK2_HUMAN DOCK2_HUMAN]] Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.<ref>PMID:21613211</ref> [[http://www.uniprot.org/uniprot/RAC1_HUMAN RAC1_HUMAN]] Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2yin" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Barford, D]]		[[Category: Barford, D]]
	[[Category: Kulkarni, K A]]		[[Category: Kulkarni, K A]]

OCA at 16:08, 24 December 2014

OCA — Wed, 24 Dec 2014 16:08:05 GMT

←Older revision		Revision as of 16:08, 24 December 2014
Line 2:		Line 2:
	<StructureSection load='2yin' size='340' side='right' caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='2yin' size='340' side='right' caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. <br>	+	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YIN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[1ryf\|1ryf]], [[2wkq\|2wkq]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[2wkr\|2wkr]], [[2wkp\|2wkp]], [[2fju\|2fju]], [[1g4u\|1g4u]], [[1i4t\|1i4t]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[1ryf\|1ryf]], [[2wkq\|2wkq]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[2wkr\|2wkr]], [[2wkp\|2wkp]], [[2fju\|2fju]], [[1g4u\|1g4u]], [[1i4t\|1i4t]]</td></tr>
-	<tr~~><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class~~='~~plainlinks~~'~~>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [http://www.ebi.ac.uk/pdbsum/2yin PDBsum]</span></td></tr>
-	~~<tr~~><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [http://www.ebi.ac.uk/pdbsum/2yin PDBsum]</span></td></tr>	+	</table>
-	<table>	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RAC1_HUMAN RAC1_HUMAN]] Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 14:
	Multiple Factors Confer Specific Cdc42 and Rac Protein Activation by Dedicator of Cytokinesis (DOCK) Nucleotide Exchange Factors.,Kulkarni K, Yang J, Zhang Z, Barford D J Biol Chem. 2011 Jul 15;286(28):25341-51. Epub 2011 May 24. PMID:21613211<ref>PMID:21613211</ref>		Multiple Factors Confer Specific Cdc42 and Rac Protein Activation by Dedicator of Cytokinesis (DOCK) Nucleotide Exchange Factors.,Kulkarni K, Yang J, Zhang Z, Barford D J Biol Chem. 2011 Jul 15;286(28):25341-51. Epub 2011 May 24. PMID:21613211<ref>PMID:21613211</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Barford, D.]]	+	[[Category: Barford, D]]
-	[[Category: Kulkarni, K A.]]	+	[[Category: Kulkarni, K A]]
-	[[Category: Yang, J.]]	+	[[Category: Yang, J]]
-	[[Category: Zhang, Z.]]	+	[[Category: Zhang, Z]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Dock]]		[[Category: Dock]]
	[[Category: Dock guanine nucleotide exchange factor]]		[[Category: Dock guanine nucleotide exchange factor]]
	[[Category: Rho gtpase]]		[[Category: Rho gtpase]]

OCA at 07:39, 14 May 2014

OCA — Wed, 14 May 2014 07:39:44 GMT

←Older revision		Revision as of 07:39, 14 May 2014
Line 1:		Line 1:
-	[[~~Image:~~2yin.~~png~~\|~~left~~\|~~200px~~]]	+	==STRUCTURE OF THE COMPLEX BETWEEN DOCK2 AND RAC1.==
		+	<StructureSection load='2yin' size='340' side='right' caption='[[2yin]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2vrw\|2vrw]], [[1e96\|1e96]], [[1i4d\|1i4d]], [[1foe\|1foe]], [[1mh1\|1mh1]], [[1i4l\|1i4l]], [[1he1\|1he1]], [[1ryf\|1ryf]], [[2wkq\|2wkq]], [[1ryh\|1ryh]], [[1hh4\|1hh4]], [[2wkr\|2wkr]], [[2wkp\|2wkp]], [[2fju\|2fju]], [[1g4u\|1g4u]], [[1i4t\|1i4t]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yin FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yin OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yin RCSB], [http://www.ebi.ac.uk/pdbsum/2yin PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DOCK (dedicator of cytokinesis) guanine nucleotide exchange factors (GEFs) activate the Rho-family GTPases Rac and Cdc42 to control cell migration, morphogenesis, and phagocytosis. The DOCK A and B subfamilies activate Rac, whereas the DOCK D subfamily activates Cdc42. Nucleotide exchange is catalyzed by a conserved DHR2 domain (DOCK(DHR2)). Although the molecular basis for DOCK(DHR2)-mediated GTPase activation has been elucidated through structures of a DOCK9(DHR2)-Cdc42 complex, the factors determining recognition of specific GTPases are unknown. To understand the molecular basis for DOCK-GTPase specificity, we have determined the crystal structure of DOCK2(DHR2) in complex with Rac1. DOCK2(DHR2) and DOCK9(DHR2) exhibit similar tertiary structures and homodimer interfaces and share a conserved GTPase-activating mechanism. Multiple structural differences between DOCK2(DHR2) and DOCK9(DHR2) account for their selectivity toward Rac1 and Cdc42. Key determinants of selectivity of Cdc42 and Rac for their cognate DOCK(DHR2) are a Phe or Trp residue within beta3 (residue 56) and the ability of DOCK proteins to exploit differences in the GEF-induced conformational changes of switch 1 dependent on a divergent residue at position 27. DOCK proteins, therefore, differ from DH-PH GEFs that select their cognate GTPases through recognition of structural differences within the beta2/beta3 strands.

-	~~<!--~~	+	Multiple Factors Confer Specific Cdc42 and Rac Protein Activation by Dedicator of Cytokinesis (DOCK) Nucleotide Exchange Factors.,Kulkarni K, Yang J, Zhang Z, Barford D J Biol Chem. 2011 Jul 15;286(28):25341-51. Epub 2011 May 24. PMID:21613211<ref>PMID:21613211</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_2yin"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_2yin\| PDB=2yin \| SCENE= }}~~	+

-	~~===STRUCTURE OF THE COMPLEX BETWEEN DOCK2 AND RAC1.===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21613211}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21613211 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21613211}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2yin]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021613211<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]

OCA at 04:41, 27 July 2011

OCA — Wed, 27 Jul 2011 04:41:01 GMT

←Older revision		Revision as of 04:41, 27 July 2011
Line 1:		Line 1:
-	[[Image:2yin.~~jpg~~\|left\|200px]]	+	[[Image:2yin.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===STRUCTURE OF THE COMPLEX BETWEEN DOCK2 AND RAC1.===		===STRUCTURE OF THE COMPLEX BETWEEN DOCK2 AND RAC1.===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21613211}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21613211 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21613211}}

	==About this Structure==		==About this Structure==
	[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA].		[[2yin]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YIN OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021613211</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Barford, D.]]		[[Category: Barford, D.]]
Line 19:		Line 28:
	[[Category: Yang, J.]]		[[Category: Yang, J.]]
	[[Category: Zhang, Z.]]		[[Category: Zhang, Z.]]
		+	[[Category: Apoptosis]]
		+	[[Category: Dock]]
		+	[[Category: Dock guanine nucleotide exchange factor]]
		+	[[Category: Rho gtpase]]

OCA: Protected "2yin" [edit=sysop:move=sysop]

OCA — Wed, 25 May 2011 05:41:19 GMT

Protected "2yin" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:41, 25 May 2011