2yjg - Revision history

OCA at 07:12, 1 May 2024

OCA — Wed, 01 May 2024 07:12:13 GMT

←Older revision		Revision as of 07:12, 1 May 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/LARA_THETC LARA_THETC] Catalyzes the interconversion between the D- and L-isomers of lactate.<ref>PMID:24710389</ref>		[https://www.uniprot.org/uniprot/LARA_THETC LARA_THETC] Catalyzes the interconversion between the D- and L-isomers of lactate.<ref>PMID:24710389</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Racemases catalyse the inversion of stereochemistry in biological molecules, giving the organism the ability to use both isomers. Among them, lactate racemase remains unexplored due to its intrinsic instability and lack of molecular characterization. Here we determine the genetic basis of lactate racemization in Lactobacillus plantarum. We show that, unexpectedly, the racemase is a nickel-dependent enzyme with a novel alpha/beta fold. In addition, we decipher the process leading to an active enzyme, which involves the activation of the apo-enzyme by a single nickel-containing maturation protein that requires preactivation by two other accessory proteins. Genomic investigations reveal the wide distribution of the lactate racemase system among prokaryotes, showing the high significance of both lactate enantiomers in carbon metabolism. The even broader distribution of the nickel-based maturation system suggests a function beyond activation of the lactate racemase and possibly linked with other undiscovered nickel-dependent enzymes.
-
-	Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system.,Desguin B, Goffin P, Viaene E, Kleerebezem M, Martin-Diaconescu V, Maroney MJ, Declercq JP, Soumillion P, Hols P Nat Commun. 2014 Apr 7;5:3615. doi: 10.1038/ncomms4615. PMID:24710389<ref>PMID:24710389</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2yjg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:14, 23 August 2023

OCA — Wed, 23 Aug 2023 08:14:44 GMT

←Older revision		Revision as of 08:14, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='2yjg' size='340' side='right'caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2yjg' size='340' side='right'caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thermoanaerobacterium_thermosaccharolyticum~~ Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum_DSM_571 Thermoanaerobacterium thermosaccharolyticum DSM 571]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YJG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [https://pdbe.org/2yjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [https://www.ebi.ac.uk/pdbsum/2yjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [https://pdbe.org/2yjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [https://www.ebi.ac.uk/pdbsum/2yjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LARA_THETC LARA_THETC] Catalyzes the interconversion between the D- and L-isomers of lactate.<ref>PMID:24710389</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Lactate racemase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Thermoanaerobacterium thermosaccharolyticum]]	+	[[Category: Thermoanaerobacterium thermosaccharolyticum DSM 571]]
-	[[Category: Declercq~~, J P~~]]	+	[[Category: Declercq JP]]
-	[[Category: Desguin, B]]	+	[[Category: Desguin B]]
-	[[Category: Hols, P]]	+	[[Category: Hols P]]
-	[[Category: Soumillion, P]]	+	[[Category: Soumillion P]]
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Nickel-dependent enzyme~~]]	+

OCA at 15:06, 17 November 2021

OCA — Wed, 17 Nov 2021 15:06:13 GMT

←Older revision		Revision as of 15:06, 17 November 2021
Line 3:		Line 3:
	<StructureSection load='2yjg' size='340' side='right'caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2yjg' size='340' side='right'caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2YJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YJG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [~~http~~://pdbe.org/2yjg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2yjg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [https://pdbe.org/2yjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [https://www.ebi.ac.uk/pdbsum/2yjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:28, 10 May 2019

OCA — Fri, 10 May 2019 12:28:40 GMT

←Older revision		Revision as of 12:28, 10 May 2019
Line 1:		Line 1:

	==Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum==		==Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum==
-	<StructureSection load='2yjg' size='340' side='right' caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='2yjg' size='340' side='right'caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YJG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YJG FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Lactate racemase]]		[[Category: Lactate racemase]]
		+	[[Category: Large Structures]]
	[[Category: Thermoanaerobacterium thermosaccharolyticum]]		[[Category: Thermoanaerobacterium thermosaccharolyticum]]
	[[Category: Declercq, J P]]		[[Category: Declercq, J P]]

OCA at 16:01, 11 August 2016

OCA — Thu, 11 Aug 2016 16:01:56 GMT

←Older revision		Revision as of 16:01, 11 August 2016
Line 1:		Line 1:
		+
	==Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum==		==Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum==
	<StructureSection load='2yjg' size='340' side='right' caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2yjg' size='340' side='right' caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [http://www.ebi.ac.uk/pdbsum/2yjg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [http://pdbe.org/2yjg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [http://www.ebi.ac.uk/pdbsum/2yjg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2yjg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:33, 5 January 2015

OCA — Mon, 05 Jan 2015 12:33:19 GMT

←Older revision		Revision as of 12:33, 5 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YJG FirstGlance]. <br>		<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YJG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [http://www.ebi.ac.uk/pdbsum/2yjg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [http://www.ebi.ac.uk/pdbsum/2yjg PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:
	[[Category: Lactate racemase]]		[[Category: Lactate racemase]]
	[[Category: Thermoanaerobacterium thermosaccharolyticum]]		[[Category: Thermoanaerobacterium thermosaccharolyticum]]
-	[[Category: Declercq, J P.]]	+	[[Category: Declercq, J P]]
-	[[Category: Desguin, B.]]	+	[[Category: Desguin, B]]
-	[[Category: Hols, P.]]	+	[[Category: Hols, P]]
-	[[Category: Soumillion, P.]]	+	[[Category: Soumillion, P]]
	[[Category: Isomerase]]		[[Category: Isomerase]]
	[[Category: Nickel-dependent enzyme]]		[[Category: Nickel-dependent enzyme]]

OCA at 06:40, 30 July 2014

OCA — Wed, 30 Jul 2014 06:40:22 GMT

←Older revision		Revision as of 06:40, 30 July 2014
Line 2:		Line 2:
	<StructureSection load='2yjg' size='340' side='right' caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='2yjg' size='340' side='right' caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. <br>	+	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2YJG FirstGlance]. <br>
	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>		</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/~~Glucokinase Glucokinase~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.7~~.1.2 2.7.1.2] </span></td></tr>	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [http://www.ebi.ac.uk/pdbsum/2yjg PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [http://www.ebi.ac.uk/pdbsum/2yjg PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Racemases catalyse the inversion of stereochemistry in biological molecules, giving the organism the ability to use both isomers. Among them, lactate racemase remains unexplored due to its intrinsic instability and lack of molecular characterization. Here we determine the genetic basis of lactate racemization in Lactobacillus plantarum. We show that, unexpectedly, the racemase is a nickel-dependent enzyme with a novel alpha/beta fold. In addition, we decipher the process leading to an active enzyme, which involves the activation of the apo-enzyme by a single nickel-containing maturation protein that requires preactivation by two other accessory proteins. Genomic investigations reveal the wide distribution of the lactate racemase system among prokaryotes, showing the high significance of both lactate enantiomers in carbon metabolism. The even broader distribution of the nickel-based maturation system suggests a function beyond activation of the lactate racemase and possibly linked with other undiscovered nickel-dependent enzymes.
		+
		+	Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system.,Desguin B, Goffin P, Viaene E, Kleerebezem M, Martin-Diaconescu V, Maroney MJ, Declercq JP, Soumillion P, Hols P Nat Commun. 2014 Apr 7;5:3615. doi: 10.1038/ncomms4615. PMID:24710389<ref>PMID:24710389</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:45, 14 May 2014

OCA — Wed, 14 May 2014 07:45:42 GMT

←Older revision		Revision as of 07:45, 14 May 2014
Line 1:		Line 1:
-	~~[[Image:2yjg.jpg\|left\|200px]]~~	+	==Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum==
-		+	<StructureSection load='2yjg' size='340' side='right' caption='[[2yjg]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~<!--~~	+	== Structural highlights ==
-	~~The line below this paragraph, containing "STRUCTURE_2yjg", creates the "Structure Box" on the page.~~	+	<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. <br>
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
-	~~or leave the SCENE parameter empty for the default display.~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [http://www.ebi.ac.uk/pdbsum/2yjg PDBsum]</span></td></tr>
-	~~-->~~	+	<table>
-	~~{{STRUCTURE_2yjg\| PDB=2yjg \| SCENE= }}~~	+	__TOC__
-		+	</StructureSection>
-	===Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum===	+
-		+
-		+
-	==~~About this Structure~~==	+
-	[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA].	+
	[[Category: Lactate racemase]]		[[Category: Lactate racemase]]
	[[Category: Thermoanaerobacterium thermosaccharolyticum]]		[[Category: Thermoanaerobacterium thermosaccharolyticum]]

OCA at 05:58, 30 May 2012

OCA — Wed, 30 May 2012 05:58:01 GMT

←Older revision		Revision as of 05:58, 30 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2yjg.jpg\|left\|200px]]

-	The ~~entry 2yjg~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2yjg", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2yjg\| PDB=2yjg \| SCENE= }}

-	~~Authors: DECLERCQ, J.P., DESGUIN, B., SOUMILLION, P., HOLS, P.~~	+	===Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum===

-	~~Description:~~ Structure ~~of the lactate racemase apoprotein~~ from Thermoanaerobacterium thermosaccharolyticum	+
		+	==About this Structure==
		+	[[2yjg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA].
		+	[[Category: Lactate racemase]]
		+	[[Category: Thermoanaerobacterium thermosaccharolyticum]]
		+	[[Category: Declercq, J P.]]
		+	[[Category: Desguin, B.]]
		+	[[Category: Hols, P.]]
		+	[[Category: Soumillion, P.]]
		+	[[Category: Isomerase]]
		+	[[Category: Nickel-dependent enzyme]]

OCA at 05:41, 15 June 2011

OCA — Wed, 15 Jun 2011 05:41:44 GMT

←Older revision		Revision as of 05:41, 15 June 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 2yjg is ON HOLD	+	The entry 2yjg is ON HOLD until Paper Publication

	Authors: DECLERCQ, J.P., DESGUIN, B., SOUMILLION, P., HOLS, P.		Authors: DECLERCQ, J.P., DESGUIN, B., SOUMILLION, P., HOLS, P.

	Description: Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum		Description: Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum