2zat - Revision history

OCA at 13:28, 1 November 2023

OCA — Wed, 01 Nov 2023 13:28:52 GMT

←Older revision		Revision as of 13:28, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='2zat' size='340' side='right'caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2zat' size='340' side='right'caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pig Pig~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAP:NADP+NICOTINAMIDE~~-~~ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [~~http~~://pdbe.org/2zat PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zat PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zat ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [https://pdbe.org/2zat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [https://www.ebi.ac.uk/pdbsum/2zat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zat ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DHRS4_PIG DHRS4_PIG]] Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones.<ref>PMID:12604222</ref>	+	[https://www.uniprot.org/uniprot/DHRS4_PIG DHRS4_PIG] Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones.<ref>PMID:12604222</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pig~~]]	+	[[Category: Sus scrofa]]
-	[[Category: Aoki, K]]	+	[[Category: Aoki K]]
-	[[Category: Nakamura~~, K T~~]]	+	[[Category: Nakamura KT]]
-	[[Category: Tanaka, N]]	+	[[Category: Tanaka N]]
-	~~[[Category: Alpha/beta]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 12:01, 29 July 2020

OCA — Wed, 29 Jul 2020 12:01:09 GMT

←Older revision		Revision as of 12:01, 29 July 2020
Line 1:		Line 1:

	==Crystal structure of a mammalian reductase==		==Crystal structure of a mammalian reductase==
-	<StructureSection load='2zat' size='340' side='right' caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='2zat' size='340' side='right'caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://pdbe.org/2zat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zat ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://pdbe.org/2zat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zat ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pig]]		[[Category: Pig]]
	[[Category: Aoki, K]]		[[Category: Aoki, K]]

OCA at 09:10, 17 October 2018

OCA — Wed, 17 Oct 2018 09:10:18 GMT

←Older revision		Revision as of 09:10, 17 October 2018
Line 1:		Line 1:
		+
	==Crystal structure of a mammalian reductase==		==Crystal structure of a mammalian reductase==
	<StructureSection load='2zat' size='340' side='right' caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2zat' size='340' side='right' caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://pdbe.org/2zat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://pdbe.org/2zat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zat ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/za/2zat_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/za/2zat_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:01, 7 February 2016

OCA — Sun, 07 Feb 2016 11:01:58 GMT

←Older revision		Revision as of 11:01, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='2zat' size='340' side='right' caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2zat' size='340' side='right' caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sus_scrofa Sus scrofa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://pdbe.org/2zat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zat ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zat" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sus scrofa~~]]	+	[[Category: Pig]]
	[[Category: Aoki, K]]		[[Category: Aoki, K]]
	[[Category: Nakamura, K T]]		[[Category: Nakamura, K T]]

OCA at 14:35, 24 December 2014

OCA — Wed, 24 Dec 2014 14:35:27 GMT

←Older revision		Revision as of 14:35, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>		<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DHRS4_PIG DHRS4_PIG]] Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones.<ref>PMID:12604222</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Aoki, K.]]	+	[[Category: Aoki, K]]
-	[[Category: Nakamura, K T.]]	+	[[Category: Nakamura, K T]]
-	[[Category: Tanaka, N.]]	+	[[Category: Tanaka, N]]
	[[Category: Alpha/beta]]		[[Category: Alpha/beta]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 04:57, 2 October 2014

OCA — Thu, 02 Oct 2014 04:57:18 GMT

←Older revision		Revision as of 04:57, 2 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2zat.~~png\|left~~\|200px]]	+	==Crystal structure of a mammalian reductase==
		+	<StructureSection load='2zat' size='340' side='right' caption='[[2zat]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zat]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZAT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carbonyl_reductase_(NADPH) Carbonyl reductase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.184 1.1.1.184] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zat OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zat RCSB], [http://www.ebi.ac.uk/pdbsum/2zat PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/za/2zat_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pig heart peroxisomal carbonyl reductase (PerCR) belongs to the short-chain dehydrogenase/reductase family, and its sequence comprises a C-terminal SRL tripeptide, which is a variant of the type 1 peroxisomal targeting signal (PTS1) Ser-Lys-Leu. PerCR is imported into peroxisomes of HeLa cells when the cells are transfected with vectors expressing the enzyme. However, PerCR does not show specific targeting when introduced into the cells with a protein transfection reagent. To understand the structural basis for peroxisomal localization of PerCR, we determined the crystal structure of PerCR. Our data revealed that the C-terminal PTS1 of each subunit of PerCR was involved in intersubunit interactions and was buried in the interior of the tetrameric molecule. These findings indicate that the PTS1 receptor Pex5p in the cytosol recognizes the monomeric form of PerCR whose C-terminal PTS1 is exposed, and that this PerCR is targeted into the peroxisome, thereby forming a tetramer.

-	~~{{STRUCTURE_2zat\| PDB=2zat \| SCENE= }}~~	+	Molecular basis for peroxisomal localization of tetrameric carbonyl reductase.,Tanaka N, Aoki K, Ishikura S, Nagano M, Imamura Y, Hara A, Nakamura KT Structure. 2008 Mar;16(3):388-97. PMID:18334214<ref>PMID:18334214</ref>

-	~~===Crystal structure~~ of ~~a mammalian reductase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18334214}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2zat]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sus_scrofa Sus scrofa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018334214<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Aoki, K.]]		[[Category: Aoki, K.]]

OCA: Protected "2zat" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 20:52:24 GMT

Protected "2zat" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:52, 7 January 2013

OCA at 20:52, 7 January 2013

OCA — Mon, 07 Jan 2013 20:52:21 GMT

←Older revision		Revision as of 20:52, 7 January 2013
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-	{{Seed}}
	[[Image:2zat.png\|left\|200px]]		[[Image:2zat.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2zat", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2zat\| PDB=2zat \| SCENE= }}		{{STRUCTURE_2zat\| PDB=2zat \| SCENE= }}

	===Crystal structure of a mammalian reductase===		===Crystal structure of a mammalian reductase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18334214}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18334214 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18334214}}		{{ABSTRACT_PUBMED_18334214}}

	==About this Structure==		==About this Structure==
-	~~2ZAT~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA].	+	[[2zat]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18334214~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018334214</ref><references group="xtra"/>
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Aoki, K.]]		[[Category: Aoki, K.]]
Line 30:		Line 18:
	[[Category: Alpha/beta]]		[[Category: Alpha/beta]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:21:42 2009''

OCA at 11:21, 16 February 2009

OCA — Mon, 16 Feb 2009 11:21:44 GMT

←Older revision		Revision as of 11:21, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2ZAT is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA].	+	2ZAT is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZAT OCA].

	==Reference==		==Reference==
-	~~Molecular basis for peroxisomal localization of tetrameric carbonyl reductase., Tanaka N, Aoki K, Ishikura S, Nagano M, Imamura Y, Hara A, Nakamura KT, Structure. 2008 Mar;16(3):388-97.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18334214 18334214]~~	+	<ref group="xtra">PMID:18334214</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Aoki, K.]]		[[Category: Aoki, K.]]
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	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 15~~:46:~~12 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:21:42 2009''

OCA at 12:46, 27 July 2008

OCA — Sun, 27 Jul 2008 12:46:14 GMT

←Older revision		Revision as of 12:46, 27 July 2008
Line 1:		Line 1:
-	[[Image:2zat.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2zat.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_2zat\| PDB=2zat \| SCENE= }}		{{STRUCTURE_2zat\| PDB=2zat \| SCENE= }}

-	~~'''~~Crystal structure of a mammalian reductase~~'''~~	+	===Crystal structure of a mammalian reductase===


-	~~==Overview==~~	+	<!--
-	~~Pig heart peroxisomal carbonyl reductase (PerCR) belongs to the short~~-~~chain dehydrogenase/reductase family, and its sequence comprises a C~~-~~terminal SRL tripeptide~~, ~~which is a variant of~~ the ~~type 1 peroxisomal targeting signal~~ (~~PTS1) Ser-Lys-Leu~~. ~~PerCR is imported into peroxisomes of HeLa cells when the cells are transfected with vectors expressing the enzyme~~. ~~However~~, ~~PerCR does not show specific targeting when introduced into~~ the ~~cells with a protein transfection reagent~~. ~~To understand the structural basis for peroxisomal localization of PerCR, we determined the crystal structure of PerCR. Our data revealed that the C~~-terminal PTS1 of each subunit of PerCR was involved in intersubunit interactions and was buried in the interior of the tetrameric molecule. These findings indicate that the PTS1 receptor Pex5p in the cytosol recognizes the monomeric form of PerCR whose C-~~terminal PTS1 is exposed, and that this PerCR is targeted into the peroxisome, thereby forming a tetramer.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_18334214}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18334214 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18334214}}

	==About this Structure==		==About this Structure==
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	[[Category: Alpha/beta]]		[[Category: Alpha/beta]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Apr 30 13~~:30:41 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 15:46:12 2008''