2zgw - Revision history

OCA at 13:36, 1 November 2023

OCA — Wed, 01 Nov 2023 13:36:00 GMT

←Older revision		Revision as of 13:36, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='2zgw' size='340' side='right'caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2zgw' size='340' side='right'caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pyrococcus_horikoshii~~ Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>~~, ~~<scene name='pdbligand=BTN:BIOTIN'>BTN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~bpl ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=70601 Pyrococcus horikoshii])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [https://pdbe.org/2zgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [https://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgw ProSAT], [https://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [https://pdbe.org/2zgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [https://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgw ProSAT], [https://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O57883_PYRHO O57883_PYRHO]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pyrococcus horikoshii]]	+	[[Category: Pyrococcus horikoshii OT3]]
-	[[Category: Bagautdinov, B]]	+	[[Category: Bagautdinov B]]
-	[[Category: Bagautdinova, S]]	+	[[Category: Bagautdinova S]]
-	[[Category: Kunishima, N]]	+	[[Category: Kunishima N]]
-	[[Category: Matsuura, Y]]	+	[[Category: Matsuura Y]]
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Biotin biosynthesis]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: National project on protein structural and functional analyse]]~~	+
-	~~[[Category: Nppsfa]]~~	+
-	~~[[Category: Rsgi~~]]	+

OCA at 07:15, 10 November 2021

OCA — Wed, 10 Nov 2021 07:15:57 GMT

←Older revision		Revision as of 07:15, 10 November 2021
Line 1:		Line 1:

	==Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A==		==Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A==
-	<StructureSection load='2zgw' size='340' side='right' caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='2zgw' size='340' side='right'caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [~~http~~://pdbe.org/2zgw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgw ProSAT], [~~http~~://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [https://pdbe.org/2zgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [https://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgw ProSAT], [https://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 29:		Line 29:

	==See Also==		==See Also==
-	*[[Biotin Protein Ligase\|Biotin Protein Ligase]]	+	*[[Biotin Protein Ligase 3D structures\|Biotin Protein Ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Bagautdinov, B]]		[[Category: Bagautdinov, B]]

OCA at 07:23, 18 October 2018

OCA — Thu, 18 Oct 2018 07:23:19 GMT

←Older revision		Revision as of 07:23, 18 October 2018
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/2zgw_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/2zgw_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:12, 18 October 2016

OCA — Tue, 18 Oct 2016 07:12:05 GMT

←Older revision		Revision as of 07:12, 18 October 2016
Line 3:		Line 3:
	<StructureSection load='2zgw' size='340' side='right' caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2zgw' size='340' side='right' caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrho Pyrho~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 ~~PYRHO~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [http://pdbe.org/2zgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [http://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgw ProSAT], [http://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [http://pdbe.org/2zgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [http://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgw ProSAT], [http://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>
	</table>		</table>
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pyrho~~]]	+	[[Category: Pyrococcus horikoshii]]
	[[Category: Bagautdinov, B]]		[[Category: Bagautdinov, B]]
	[[Category: Bagautdinova, S]]		[[Category: Bagautdinova, S]]

OCA at 12:59, 11 August 2016

OCA — Thu, 11 Aug 2016 12:59:59 GMT

←Older revision		Revision as of 12:59, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A==		==Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A==
	<StructureSection load='2zgw' size='340' side='right' caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='2zgw' size='340' side='right' caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_horikoshii Pyrococcus horikoshii~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrho Pyrho]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~53953 Pyrococcus horikoshii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 PYRHO])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [http://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [http://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [http://pdbe.org/2zgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [http://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zgw ProSAT], [http://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zgw ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zgw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pyrococcus horikoshii~~]]	+	[[Category: Pyrho]]
	[[Category: Bagautdinov, B]]		[[Category: Bagautdinov, B]]
	[[Category: Bagautdinova, S]]		[[Category: Bagautdinova, S]]

OCA at 10:41, 20 January 2015

OCA — Tue, 20 Jan 2015 10:41:04 GMT

←Older revision		Revision as of 10:41, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>		<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [http://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [http://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [http://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [http://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
-	[[Category: Bagautdinov, B.]]	+	[[Category: Bagautdinov, B]]
-	[[Category: Bagautdinova, S.]]	+	[[Category: Bagautdinova, S]]
-	[[Category: Kunishima, N.]]	+	[[Category: Kunishima, N]]
-	[[Category: Matsuura, Y.]]	+	[[Category: Matsuura, Y]]
-	[[Category: ~~RSGI, RIKEN~~ Structural ~~Genomics/Proteomics Initiative.~~]]	+	[[Category: Structural genomic]]
	[[Category: Biotin biosynthesis]]		[[Category: Biotin biosynthesis]]
	[[Category: Dimer]]		[[Category: Dimer]]
Line 43:		Line 43:
	[[Category: National project on protein structural and functional analyse]]		[[Category: National project on protein structural and functional analyse]]
	[[Category: Nppsfa]]		[[Category: Nppsfa]]
-	[[Category: Riken structural genomics/proteomics initiative]]
	[[Category: Rsgi]]		[[Category: Rsgi]]
-	[[Category: Structural genomic]]

OCA at 09:09, 29 September 2014

OCA — Mon, 29 Sep 2014 09:09:42 GMT

←Older revision		Revision as of 09:09, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2zgw.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A==
		+	<StructureSection load='2zgw' size='340' side='right' caption='[[2zgw]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZGW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zgw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zgw RCSB], [http://www.ebi.ac.uk/pdbsum/2zgw PDBsum], [http://www.topsan.org/Proteins/RSGI/2zgw TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/2zgw_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Biotin protein ligase (BPL) catalyzes the biotinylation of the biotin carboxyl carrier protein (BCCP) only at a special lysine residue. Here we report the first structure of BPL.BCCP complex crystals, which are prepared using two BPL mutants: R48A and R48A/K111A. From a detailed structural characterization, it is likely that the mutants retain functionality as enzymes but have a reduced activity to produce the reaction intermediate biotinyl-5'-AMP. The observed biotin and partly disordered ATP in the mutant structures may act as a non-reactive analog of the substrates or biotinyl-5'-AMP, thereby providing the complex crystals. The four crystallographically independent BPL.BCCP complexes obtained can be classified structurally into three groups: the formation stages 1 and 2 with apo-BCCP and the product stage with biotinylated holo-BCCP. Residues responsible for the complex formation as well as for the biotinylation reaction have been identified. The C-terminal domain of BPL shows especially large conformational changes to accommodate BCCP, suggesting its functional importance. The formation stage 1 complex shows the closest distance between the carboxyl carbon of biotin and the special lysine of BCCP, suggesting its relevance to the unobserved reaction stage. Interestingly, bound ATP and biotin are also seen in the product stage, indicating that the substrates may be recruited into the product stage complex before the release of holo-BCCP, probably for the next reaction cycle. The existence of formation and product stages before and after the reaction stage would be favorable to ensure both the reaction efficiency and the extreme substrate specificity of the biotinylation reaction.

-	~~{{STRUCTURE_2zgw\| PDB=2zgw \| SCENE= }}~~	+	Protein biotinylation visualized by a complex structure of biotin protein ligase with a substrate.,Bagautdinov B, Matsuura Y, Bagautdinova S, Kunishima N J Biol Chem. 2008 May 23;283(21):14739-50. Epub 2008 Mar 26. PMID:18372281<ref>PMID:18372281</ref>

-	~~===Crystal Structure Of Biotin Protein Ligase~~ From ~~Pyrococcus Horikoshii Complexed with Adenosine and Biotin~~, ~~Mutations R48A and K111A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18372281}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2zgw]] is~~ a ~~2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes~~ the ~~now removed PDB entry [http://oca~~.~~weizmann~~.ac.il/~~oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA].~~	+

	==See Also==		==See Also==
	*[[Biotin Protein Ligase\|Biotin Protein Ligase]]		*[[Biotin Protein Ligase\|Biotin Protein Ligase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:018372281<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Bagautdinov, B.]]		[[Category: Bagautdinov, B.]]

OCA at 19:56, 20 October 2012

OCA — Sat, 20 Oct 2012 19:56:52 GMT

←Older revision		Revision as of 19:56, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2zgw]] is a 2 chain structure ~~of [[Biotin Protein Ligase]]~~ with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA].	+	[[2zgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA].

	==See Also==		==See Also==

OCA: Protected "2zgw" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 08:58:08 GMT

Protected "2zgw" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:58, 27 July 2012

OCA at 08:58, 27 July 2012

OCA — Fri, 27 Jul 2012 08:58:06 GMT

←Older revision		Revision as of 08:58, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2zgw.png\|left\|200px]]		[[Image:2zgw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2zgw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2zgw\| PDB=2zgw \| SCENE= }}		{{STRUCTURE_2zgw\| PDB=2zgw \| SCENE= }}

	===Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A===		===Crystal Structure Of Biotin Protein Ligase From Pyrococcus Horikoshii Complexed with Adenosine and Biotin, Mutations R48A and K111A===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18372281}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18372281 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18372281}}		{{ABSTRACT_PUBMED_18372281}}

	==About this Structure==		==About this Structure==
-	~~2ZGW~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA].	+	[[2zgw]] is a 2 chain structure of [[Biotin Protein Ligase]] with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2e3y 2e3y]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZGW OCA].
		+
		+	==See Also==
		+	*[[Biotin Protein Ligase\|Biotin Protein Ligase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18372281~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018372281</ref><references group="xtra"/>
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
	[[Category: Bagautdinov, B.]]		[[Category: Bagautdinov, B.]]
Line 38:		Line 29:
	[[Category: Rsgi]]		[[Category: Rsgi]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
-	[[Category: X-ray diffraction]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:13:52 2009''