2zit - Revision history

OCA at 06:33, 3 April 2024

OCA — Wed, 03 Apr 2024 06:33:16 GMT

←Older revision		Revision as of 06:33, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='2zit' size='340' side='right'caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2zit' size='340' side='right'caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~] and [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAD:NICOTINAMIDE~~-~~ADENINE-DINUCLEOTIDE'>NAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;~~'>~~[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[3b8h\|3b8h]], [[3b78\|3b78]], [[3b82\|3b82]]~~</~~div~~>~~</td></tr>~~	+
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ToxA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [https://pdbe.org/2zit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [https://www.ebi.ac.uk/pdbsum/2zit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zit ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [https://pdbe.org/2zit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [https://www.ebi.ac.uk/pdbsum/2zit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zit ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~TOXA_PSEAE TOXA_PSEAE~~]] An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.<ref>PMID:18276581</ref>	+	[https://www.uniprot.org/uniprot/EF2_YEAST EF2_YEAST]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zit ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zit ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Exotoxin A (ExoA) from Pseudomonas aeruginosa is an important virulence factor that belongs to a class of exotoxins that are secreted by pathogenic bacteria which cause human diseases such as cholera, diphtheria, pneumonia and whooping cough. We present the first crystal structures, to our knowledge, of ExoA in complex with elongation factor 2 (eEF2) and intact NAD(+), which indicate a direct role of two active-site loops in ExoA during the catalytic cycle. One loop moves to form a solvent cover for the active site of the enzyme and reaches towards the target residue (diphthamide) in eEF2 forming an important hydrogen bond. The NAD(+) substrate adopts a conformation remarkably different from that of the NAD(+) analogue, betaTAD, observed in previous structures, and fails to trigger any loop movements. Mutational studies of the two loops in the toxin identify several residues important for catalytic activity, in particular Glu 546 and Arg 551, clearly supporting the new complex structures. On the basis of these data, we propose a transition-state model for the toxin-catalysed reaction.
-
-	The nature and character of the transition state for the ADP-ribosyltransferase reaction.,Jorgensen R, Wang Y, Visschedyk D, Merrill AR EMBO Rep. 2008 Aug;9(8):802-9. Epub 2008 Jun 27. PMID:18583986<ref>PMID:18583986</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2zit" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]		*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
	*[[Exotoxin 3D structures\|Exotoxin 3D structures]]		*[[Exotoxin 3D structures\|Exotoxin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Jorgensen, R~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Merrill, A R~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Adp-ribosylation~~]]	+	[[Category: Jorgensen R]]
-	[[Category: ~~Biosynthetic protein-transferase complex]]~~	+	[[Category: Merrill AR]]
-	~~[[Category: Cytoplasm]]~~	+
-	~~[[Category: Elongation factor]]~~	+
-	~~[[Category: Glycosyltransferase]]~~	+
-	~~[[Category: Gtp-binding]]~~	+
-	~~[[Category: Nad]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Protein biosynthesis]]~~	+
-	~~[[Category: Rna-binding]]~~	+
-	~~[[Category: Rrna-binding]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Toxin-substrate complex]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 17:30, 15 December 2021

OCA — Wed, 15 Dec 2021 17:30:56 GMT

←Older revision		Revision as of 17:30, 15 December 2021
Line 1:		Line 1:

	==Structure of the eEF2-ExoA-NAD+ complex==		==Structure of the eEF2-ExoA-NAD+ complex==
-	<StructureSection load='2zit' size='340' side='right' caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='2zit' size='340' side='right'caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[3b8h\|3b8h]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[3b8h\|3b8h]], [[3b78\|3b78]], [[3b82\|3b82]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ToxA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ToxA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [~~http~~://pdbe.org/2zit PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zit PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zit ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [https://pdbe.org/2zit PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [https://www.ebi.ac.uk/pdbsum/2zit PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zit ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TOXA_PSEAE TOXA_PSEAE]] An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.<ref>PMID:18276581</ref>	+	[[https://www.uniprot.org/uniprot/TOXA_PSEAE TOXA_PSEAE]] An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.<ref>PMID:18276581</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/2zit_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/2zit_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 31:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 2zit" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2zit" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
		+	*[[Exotoxin 3D structures\|Exotoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 40:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Jorgensen, R]]		[[Category: Jorgensen, R]]
	[[Category: Merrill, A R]]		[[Category: Merrill, A R]]

OCA at 15:47, 12 October 2017

OCA — Thu, 12 Oct 2017 15:47:17 GMT

←Older revision		Revision as of 15:47, 12 October 2017
Line 1:		Line 1:
		+
	==Structure of the eEF2-ExoA-NAD+ complex==		==Structure of the eEF2-ExoA-NAD+ complex==
	<StructureSection load='2zit' size='340' side='right' caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2zit' size='340' side='right' caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[3b8h\|3b8h]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[3b8h\|3b8h]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ToxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ToxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [http://pdbe.org/2zit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [http://www.ebi.ac.uk/pdbsum/2zit PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [http://pdbe.org/2zit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [http://www.ebi.ac.uk/pdbsum/2zit PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zit ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 31:
	</div>		</div>
	<div class="pdbe-citations 2zit" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 2zit" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Elongation factor\|Elongation factor]]
-	*[[Exotoxin\|Exotoxin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Jorgensen, R]]		[[Category: Jorgensen, R]]
	[[Category: Merrill, A R]]		[[Category: Merrill, A R]]
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
	[[Category: Biosynthetic protein-transferase complex]]		[[Category: Biosynthetic protein-transferase complex]]
		+	[[Category: Cytoplasm]]
	[[Category: Elongation factor]]		[[Category: Elongation factor]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]

OCA at 04:36, 9 February 2016

OCA — Tue, 09 Feb 2016 04:36:32 GMT

←Older revision		Revision as of 04:36, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2zit' size='340' side='right' caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2zit' size='340' side='right' caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[3b8h\|3b8h]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[3b8h\|3b8h]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ToxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 ~~Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ToxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [http://www.ebi.ac.uk/pdbsum/2zit PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [http://pdbe.org/2zit PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [http://www.ebi.ac.uk/pdbsum/2zit PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zit ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zit" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Jorgensen, R]]		[[Category: Jorgensen, R]]

OCA at 04:27, 25 December 2014

OCA — Thu, 25 Dec 2014 04:27:52 GMT

←Older revision		Revision as of 04:27, 25 December 2014
Line 1:		Line 1:
-	[[~~Image:~~2zit.~~png~~\|~~left~~\|200px]]	+	==Structure of the eEF2-ExoA-NAD+ complex==
		+	<StructureSection load='2zit' size='340' side='right' caption='[[2zit]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zit]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1zm2\|1zm2]], [[1zm3\|1zm3]], [[1zm4\|1zm4]], [[3b8h\|3b8h]], [[3b78\|3b78]], [[3b82\|3b82]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ToxA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zit FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zit OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zit RCSB], [http://www.ebi.ac.uk/pdbsum/2zit PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TOXA_PSEAE TOXA_PSEAE]] An NAD-dependent ADP-ribosyltransferase (ADPRT). Catalyzes the transfer of the ADP ribosyl moiety of oxidized NAD (NAD(+)) onto eukaryotic elongation factor 2 (eEF-2) thus arresting protein synthesis. Has an LD(50) of 65 ng/ml against the human lung epithelial cell line C38.<ref>PMID:18276581</ref>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/2zit_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Exotoxin A (ExoA) from Pseudomonas aeruginosa is an important virulence factor that belongs to a class of exotoxins that are secreted by pathogenic bacteria which cause human diseases such as cholera, diphtheria, pneumonia and whooping cough. We present the first crystal structures, to our knowledge, of ExoA in complex with elongation factor 2 (eEF2) and intact NAD(+), which indicate a direct role of two active-site loops in ExoA during the catalytic cycle. One loop moves to form a solvent cover for the active site of the enzyme and reaches towards the target residue (diphthamide) in eEF2 forming an important hydrogen bond. The NAD(+) substrate adopts a conformation remarkably different from that of the NAD(+) analogue, betaTAD, observed in previous structures, and fails to trigger any loop movements. Mutational studies of the two loops in the toxin identify several residues important for catalytic activity, in particular Glu 546 and Arg 551, clearly supporting the new complex structures. On the basis of these data, we propose a transition-state model for the toxin-catalysed reaction.

-	~~{{STRUCTURE_2zit\| PDB=2zit \| SCENE= }}~~	+	The nature and character of the transition state for the ADP-ribosyltransferase reaction.,Jorgensen R, Wang Y, Visschedyk D, Merrill AR EMBO Rep. 2008 Aug;9(8):802-9. Epub 2008 Jun 27. PMID:18583986<ref>PMID:18583986</ref>

-	~~===Structure~~ of the ~~eEF2-ExoA-NAD+ complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18583986}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2zit]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en~~.~~wikipedia.org~~/~~wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA].~~	+

	==See Also==		==See Also==
	*[[Elongation factor\|Elongation factor]]		*[[Elongation factor\|Elongation factor]]
-		+	*[[Exotoxin\|Exotoxin]]
-	==~~Reference~~==	+	== References ==
-	<~~ref group="xtra">PMID:018583986<~~/~~ref~~><~~references group="xtra"~~/>	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Jorgensen, R.]]	+	[[Category: Jorgensen, R]]
-	[[Category: Merrill, A R.]]	+	[[Category: Merrill, A R]]
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
	[[Category: Biosynthetic protein-transferase complex]]		[[Category: Biosynthetic protein-transferase complex]]

OCA: Protected "2zit" [edit=sysop:move=sysop]

OCA — Sat, 20 Oct 2012 12:39:56 GMT

Protected "2zit" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:39, 20 October 2012

OCA at 12:39, 20 October 2012

OCA — Sat, 20 Oct 2012 12:39:54 GMT

←Older revision		Revision as of 12:39, 20 October 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:2zit.png\|left\|200px]]		[[Image:2zit.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2zit", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2zit\| PDB=2zit \| SCENE= }}		{{STRUCTURE_2zit\| PDB=2zit \| SCENE= }}

	===Structure of the eEF2-ExoA-NAD+ complex===		===Structure of the eEF2-ExoA-NAD+ complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18583986}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18583986 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18583986}}		{{ABSTRACT_PUBMED_18583986}}

	==About this Structure==		==About this Structure==
-	~~2ZIT~~ is a 6 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA].	+	[[2zit]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA].
		+
		+	==See Also==
		+	*[[Elongation factor\|Elongation factor]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18583986~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018583986</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
Line 29:		Line 20:
	[[Category: Merrill, A R.]]		[[Category: Merrill, A R.]]
	[[Category: Adp-ribosylation]]		[[Category: Adp-ribosylation]]
-	[[Category: Biosynthetic protein/transferase complex]]	+	[[Category: Biosynthetic protein-transferase complex]]
-	~~[[Category: Cytoplasm~~]]	+
	[[Category: Elongation factor]]		[[Category: Elongation factor]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
Line 43:		Line 33:
	[[Category: Toxin-substrate complex]]		[[Category: Toxin-substrate complex]]
	[[Category: Transferase]]		[[Category: Transferase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 09:10:34 2009''

OCA at 06:10, 6 May 2009

OCA — Wed, 06 May 2009 06:10:35 GMT

←Older revision		Revision as of 06:10, 6 May 2009
Line 12:		Line 12:
	===Structure of the eEF2-ExoA-NAD+ complex===		===Structure of the eEF2-ExoA-NAD+ complex===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_18583986}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18583986 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18583986}}

	==About this Structure==		==About this Structure==
	2ZIT is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA].		2ZIT is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:18583986</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
Line 35:		Line 44:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Feb 17 06~~:31:35 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 6 09:10:34 2009''

OCA at 04:31, 17 February 2009

OCA — Tue, 17 Feb 2009 04:31:37 GMT

←Older revision		Revision as of 04:31, 17 February 2009
Line 1:		Line 1:
-	[[Image:2zit.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2zit.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2zit\| PDB=2zit \| SCENE= }}		{{STRUCTURE_2zit\| PDB=2zit \| SCENE= }}

-	~~'''~~Structure of the eEF2-ExoA-NAD+ complex~~'''~~	+	===Structure of the eEF2-ExoA-NAD+ complex===


	==About this Structure==		==About this Structure==
-	2ZIT is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA].	+	2ZIT is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA].
-	~~[[Category: Protein complex]]~~	+
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
Line 35:		Line 35:
	[[Category: Transferase]]		[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jun 25 18~~:55:~~20 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:31:35 2009''

OCA at 15:55, 25 June 2008

OCA — Wed, 25 Jun 2008 15:55:23 GMT

←Older revision		Revision as of 15:55, 25 June 2008
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:2zit.jpg\|left\|200px]]

-	The ~~entry 2zit~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2zit", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2zit\| PDB=2zit \| SCENE= }}

-	~~Authors: Jorgensen, R., Wang, Y., Visschedyk, D., Merrill, A.R.~~	+	'''Structure of the eEF2-ExoA-NAD+ complex'''

-	Description: Structure of the eEF2-ExoA-NAD+ complex

		+	==About this Structure==
		+	2ZIT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIT OCA].
		+	[[Category: Protein complex]]
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Jorgensen, R.]]
		+	[[Category: Merrill, A R.]]
		+	[[Category: Adp-ribosylation]]
		+	[[Category: Biosynthetic protein/transferase complex]]
		+	[[Category: Cytoplasm]]
		+	[[Category: Elongation factor]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Gtp-binding]]
		+	[[Category: Nad]]
		+	[[Category: Nucleotide-binding]]
		+	[[Category: Phosphoprotein]]
		+	[[Category: Protein biosynthesis]]
		+	[[Category: Rna-binding]]
		+	[[Category: Rrna-binding]]
		+	[[Category: Toxin]]
		+	[[Category: Toxin-substrate complex]]
		+	[[Category: Transferase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun ~~11 09~~:10:00 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 18:55:20 2008''