2zix - Revision history

OCA at 13:36, 1 November 2023

OCA — Wed, 01 Nov 2023 13:36:59 GMT

←Older revision		Revision as of 13:36, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='2zix' size='340' side='right'caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='2zix' size='340' side='right'caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.5Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">MUS81 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=~~5&~~id=9606 HUMAN]), EME1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [https://pdbe.org/2zix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [https://www.ebi.ac.uk/pdbsum/2zix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zix ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [https://pdbe.org/2zix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [https://www.ebi.ac.uk/pdbsum/2zix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MUS81_HUMAN MUS81_HUMAN]] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:11741546</ref> <ref>PMID:12374758</ref> <ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:15805243</ref> <ref>PMID:17289582</ref> <ref>PMID:19595721</ref> <ref>PMID:19596235</ref> [[https://www.uniprot.org/uniprot/EME1_HUMAN EME1_HUMAN]] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:17289582</ref>	+	[https://www.uniprot.org/uniprot/MUS81_HUMAN MUS81_HUMAN] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:11741546</ref> <ref>PMID:12374758</ref> <ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:15805243</ref> <ref>PMID:17289582</ref> <ref>PMID:19595721</ref> <ref>PMID:19596235</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chang~~, J H~~]]	+	[[Category: Chang JH]]
-	[[Category: Cho, Y]]	+	[[Category: Cho Y]]
-	[[Category: Choi~~, J M~~]]	+	[[Category: Choi JM]]
-	[[Category: Kim~~, J J~~]]	+	[[Category: Kim JJ]]
-	[[Category: Lee~~, J H]]~~	+	[[Category: Lee JH]]
-	~~[[Category: Alternative splicing]]~~	+
-	~~[[Category: Dna damage]]~~	+
-	~~[[Category: Dna recombination]]~~	+
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Endonuclease]]~~	+
-	~~[[Category: Helix-hairpin-helix]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Magnesium]]~~	+
-	~~[[Category: Metal-binding]]~~	+
-	~~[[Category: Nuclease]]~~	+
-	~~[[Category: Nucleus]]~~	+
-	~~[[Category: Phosphoprotein]]~~	+
-	~~[[Category: Polymorphism~~]]	+

OCA at 17:31, 15 December 2021

OCA — Wed, 15 Dec 2021 17:31:07 GMT

←Older revision		Revision as of 17:31, 15 December 2021
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	==Crystal structure of the Mus81-Eme1 complex==		==Crystal structure of the Mus81-Eme1 complex==
-	<StructureSection load='2zix' size='340' side='right' caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>	+	<StructureSection load='2zix' size='340' side='right'caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), EME1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), EME1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [~~http~~://pdbe.org/2zix PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zix PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zix ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [https://pdbe.org/2zix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [https://www.ebi.ac.uk/pdbsum/2zix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MUS81_HUMAN MUS81_HUMAN]] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:11741546</ref> <ref>PMID:12374758</ref> <ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:15805243</ref> <ref>PMID:17289582</ref> <ref>PMID:19595721</ref> <ref>PMID:19596235</ref> [[~~http~~://www.uniprot.org/uniprot/EME1_HUMAN EME1_HUMAN]] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:17289582</ref>	+	[[https://www.uniprot.org/uniprot/MUS81_HUMAN MUS81_HUMAN]] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:11741546</ref> <ref>PMID:12374758</ref> <ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:15805243</ref> <ref>PMID:17289582</ref> <ref>PMID:19595721</ref> <ref>PMID:19596235</ref> [[https://www.uniprot.org/uniprot/EME1_HUMAN EME1_HUMAN]] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:17289582</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Endonuclease\|Endonuclease]]	+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Chang, J H]]		[[Category: Chang, J H]]
	[[Category: Cho, Y]]		[[Category: Cho, Y]]

OCA at 06:51, 18 October 2018

OCA — Thu, 18 Oct 2018 06:51:29 GMT

←Older revision		Revision as of 06:51, 18 October 2018
Line 1:		Line 1:
		+
	==Crystal structure of the Mus81-Eme1 complex==		==Crystal structure of the Mus81-Eme1 complex==
	<StructureSection load='2zix' size='340' side='right' caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='2zix' size='340' side='right' caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), EME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), EME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [http://pdbe.org/2zix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [http://www.ebi.ac.uk/pdbsum/2zix PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [http://pdbe.org/2zix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [http://www.ebi.ac.uk/pdbsum/2zix PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zix ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/2zix_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/2zix_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 41:		Line 42:
	[[Category: Kim, J J]]		[[Category: Kim, J J]]
	[[Category: Lee, J H]]		[[Category: Lee, J H]]
		+	[[Category: Alternative splicing]]
	[[Category: Dna damage]]		[[Category: Dna damage]]
	[[Category: Dna recombination]]		[[Category: Dna recombination]]
Line 52:		Line 54:
	[[Category: Nucleus]]		[[Category: Nucleus]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
		+	[[Category: Polymorphism]]

OCA at 05:33, 9 February 2016

OCA — Tue, 09 Feb 2016 05:33:59 GMT

←Older revision		Revision as of 05:33, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='2zix' size='340' side='right' caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>		<StructureSection load='2zix' size='340' side='right' caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), EME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), EME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [http://www.ebi.ac.uk/pdbsum/2zix PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [http://pdbe.org/2zix PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [http://www.ebi.ac.uk/pdbsum/2zix PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zix ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zix" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Endonuclease\|Endonuclease]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Chang, J H]]		[[Category: Chang, J H]]
	[[Category: Cho, Y]]		[[Category: Cho, Y]]

OCA at 04:46, 25 December 2014

OCA — Thu, 25 Dec 2014 04:46:22 GMT

←Older revision		Revision as of 04:46, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>		<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), EME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), EME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [http://www.ebi.ac.uk/pdbsum/2zix PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [http://www.ebi.ac.uk/pdbsum/2zix PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MUS81_HUMAN MUS81_HUMAN]] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:11741546</ref> <ref>PMID:12374758</ref> <ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:15805243</ref> <ref>PMID:17289582</ref> <ref>PMID:19595721</ref> <ref>PMID:19596235</ref> [[http://www.uniprot.org/uniprot/EME1_HUMAN EME1_HUMAN]] Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:17289582</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Chang, J H.]]	+	[[Category: Chang, J H]]
-	[[Category: Cho, Y.]]	+	[[Category: Cho, Y]]
-	[[Category: Choi, J M.]]	+	[[Category: Choi, J M]]
-	[[Category: Kim, J J.]]	+	[[Category: Kim, J J]]
-	[[Category: Lee, J H.]]	+	[[Category: Lee, J H]]
	[[Category: Dna damage]]		[[Category: Dna damage]]
	[[Category: Dna recombination]]		[[Category: Dna recombination]]

OCA at 04:47, 2 October 2014

OCA — Thu, 02 Oct 2014 04:47:07 GMT

←Older revision		Revision as of 04:47, 2 October 2014
Line 1:		Line 1:
-	[[~~Image:~~2zix.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the Mus81-Eme1 complex==
		+	<StructureSection load='2zix' size='340' side='right' caption='[[2zix]], [[Resolution\|resolution]] 3.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZIX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2ziu\|2ziu]], [[2ziv\|2ziv]], [[2ziw\|2ziw]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MUS81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), EME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zix RCSB], [http://www.ebi.ac.uk/pdbsum/2zix PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zi/2zix_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Mus81-Eme1 complex is a structure-specific endonuclease that plays an important role in rescuing stalled replication forks and resolving the meiotic recombination intermediates in eukaryotes. We have determined the crystal structure of the Mus81-Eme1 complex. Both Mus81 and Eme1 consist of a central nuclease domain, two repeats of the helix-hairpin-helix (HhH) motif at their C-terminal region, and a linker helix. While each domain structure resembles archaeal XPF homologs, the overall structure is significantly different from those due to the structure of a linker helix. We show that a flexible intradomain linker that formed with 36 residues in the nuclease domain of Eme1 is essential for the recognition of DNA. We identified several basic residues lining the outer surface of the active site cleft of Mus81 that are involved in the interaction with a flexible arm of a nicked Holliday junction (HJ). These interactions might contribute to the optimal positioning of the opposite junction across the nick into the catalytic site, which provided the basis for the "nick and counternick" mechanism of Mus81-Eme1 and for the nicked HJ to be the favored in vitro substrate of this enzyme.

-	~~{{STRUCTURE_2zix\| PDB=2zix \| SCENE= }}~~	+	Crystal structure of the Mus81-Eme1 complex.,Chang JH, Kim JJ, Choi JM, Lee JH, Cho Y Genes Dev. 2008 Apr 15;22(8):1093-106. PMID:18413719<ref>PMID:18413719</ref>

-	~~===Crystal structure~~ of the ~~Mus81-Eme1 complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_18413719}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2zix]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:018413719<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Chang, J H.]]		[[Category: Chang, J H.]]

OCA: Protected "2zix" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 20:37:53 GMT

Protected "2zix" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:37, 7 January 2013

OCA at 20:37, 7 January 2013

OCA — Mon, 07 Jan 2013 20:37:50 GMT

←Older revision		Revision as of 20:37, 7 January 2013
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-	{{Seed}}
	[[Image:2zix.png\|left\|200px]]		[[Image:2zix.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2zix", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2zix\| PDB=2zix \| SCENE= }}		{{STRUCTURE_2zix\| PDB=2zix \| SCENE= }}

	===Crystal structure of the Mus81-Eme1 complex===		===Crystal structure of the Mus81-Eme1 complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_18413719}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 18413719 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_18413719}}		{{ABSTRACT_PUBMED_18413719}}

	==About this Structure==		==About this Structure==
-	~~2ZIX~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA].	+	[[2zix]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~18413719~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:018413719</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Chang, J H.]]		[[Category: Chang, J H.]]
Line 30:		Line 18:
	[[Category: Kim, J J.]]		[[Category: Kim, J J.]]
	[[Category: Lee, J H.]]		[[Category: Lee, J H.]]
-	[[Category: Alternative splicing]]
	[[Category: Dna damage]]		[[Category: Dna damage]]
	[[Category: Dna recombination]]		[[Category: Dna recombination]]
Line 42:		Line 29:
	[[Category: Nucleus]]		[[Category: Nucleus]]
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
-	[[Category: Polymorphism]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:52:44 2009''

OCA at 04:52, 17 February 2009

OCA — Tue, 17 Feb 2009 04:52:46 GMT

←Older revision		Revision as of 04:52, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	2ZIX is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA].	+	2ZIX is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZIX OCA].

	==Reference==		==Reference==
-	~~Crystal structure of the Mus81-Eme1 complex., Chang JH, Kim JJ, Choi JM, Lee JH, Cho Y, Genes Dev. 2008 Apr 15;22(8):1093-106.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/18413719 18413719]~~	+	<ref group="xtra">PMID:18413719</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Protein complex]]
	[[Category: Chang, J H.]]		[[Category: Chang, J H.]]
	[[Category: Cho, Y.]]		[[Category: Cho, Y.]]
Line 45:		Line 44:
	[[Category: Polymorphism]]		[[Category: Polymorphism]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 10~~:31:~~05 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:52:44 2009''

OCA at 07:31, 28 July 2008

OCA — Mon, 28 Jul 2008 07:31:12 GMT

←Older revision		Revision as of 07:31, 28 July 2008
Line 1:		Line 1:
-	[[Image:2zix.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:2zix.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_2zix\| PDB=2zix \| SCENE= }}		{{STRUCTURE_2zix\| PDB=2zix \| SCENE= }}

-	~~'''~~Crystal structure of the Mus81-Eme1 complex~~'''~~	+	===Crystal structure of the Mus81-Eme1 complex===


-	~~==Overview==~~	+	<!--
-	The Mus81-Eme1 complex is a structure-specific endonuclease that plays an important role in rescuing stalled replication forks and resolving the meiotic recombination intermediates in eukaryotes. We have determined the crystal structure of the Mus81-Eme1 complex. Both Mus81 and Eme1 consist of a central nuclease domain, ~~two repeats of the helix-hairpin-helix (HhH) motif at their C-terminal region, and a linker helix. While each domain structure resembles archaeal XPF homologs~~, the ~~overall structure is significantly different from those due~~ to the ~~structure of a linker helix~~. ~~We show that a flexible intradomain linker that formed with 36 residues in the nuclease domain of Eme1 is essential for the recognition of DNA~~. ~~We identified several basic residues lining the outer surface of the active site cleft of Mus81 that are involved in the interaction with a flexible arm of a nicked Holliday junction (HJ~~)~~. These interactions might contribute to the optimal positioning of the opposite junction across the nick into the catalytic site~~, ~~which provided~~ the ~~basis for the "nick and counternick" mechanism of Mus81~~-~~Eme1 and for the nicked HJ to be the favored in vitro substrate of this enzyme.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_18413719}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 18413719 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_18413719}}

	==About this Structure==		==About this Structure==
Line 40:		Line 44:
	[[Category: Phosphoprotein]]		[[Category: Phosphoprotein]]
	[[Category: Polymorphism]]		[[Category: Polymorphism]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Apr 30 13~~:32:02 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:31:05 2008''