2zr0 - Revision history

OCA at 13:50, 1 November 2023

OCA — Wed, 01 Nov 2023 13:50:17 GMT

←Older revision		Revision as of 13:50, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='2zr0' size='340' side='right'caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2zr0' size='340' side='right'caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Mycs2 Mycs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1ubc\|1ubc]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [https://pdbe.org/2zr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [https://www.ebi.ac.uk/pdbsum/2zr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zr0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [https://pdbe.org/2zr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [https://www.ebi.ac.uk/pdbsum/2zr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zr0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RECA_MYCS2 RECA_MYCS2]] Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.<ref>PMID:17360246</ref> <ref>PMID:17496093</ref> <ref>PMID:21219454</ref>	+	[https://www.uniprot.org/uniprot/RECA_MYCS2 RECA_MYCS2] Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.<ref>PMID:17360246</ref> <ref>PMID:17496093</ref> <ref>PMID:21219454</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 31:

	==See Also==		==See Also==
-	*[[~~Recombinase~~ A\|~~Recombinase~~ A]]	+	*[[3D structures of recombinase A\|3D structures of recombinase A]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Deleted entry]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mycs2~~]]	+	[[Category: Mycolicibacterium smegmatis MC2 155]]
-	[[Category: Chandra~~, N R~~]]	+	[[Category: Chandra NR]]
-	[[Category: Manjunath~~, G P~~]]	+	[[Category: Manjunath GP]]
-	[[Category: Muniyappa, K]]	+	[[Category: Muniyappa K]]
-	[[Category: Prabu~~, J R~~]]	+	[[Category: Prabu JR]]
-	[[Category: Vijayan, M]]	+	[[Category: Vijayan M]]
-	~~[[Category: Atp-binding]]~~	+
-	~~[[Category: Dna damage]]~~	+
-	~~[[Category: Dna recombination]]~~	+
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Dna-repair]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Nucleotide-binding]]~~	+
-	~~[[Category: Reca mutant]]~~	+
-	~~[[Category: Recombination]]~~	+
-	~~[[Category: Sos response~~]]	+

OCA at 07:19, 10 November 2021

OCA — Wed, 10 Nov 2021 07:19:19 GMT

←Older revision		Revision as of 07:19, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='2zr0' size='340' side='right'caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2zr0' size='340' side='right'caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ubc\|1ubc]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [~~http~~://pdbe.org/2zr0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zr0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zr0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [https://pdbe.org/2zr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [https://www.ebi.ac.uk/pdbsum/2zr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zr0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RECA_MYCS2 RECA_MYCS2]] Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.<ref>PMID:17360246</ref> <ref>PMID:17496093</ref> <ref>PMID:21219454</ref>	+	[[https://www.uniprot.org/uniprot/RECA_MYCS2 RECA_MYCS2]] Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.<ref>PMID:17360246</ref> <ref>PMID:17496093</ref> <ref>PMID:21219454</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:08, 29 July 2020

OCA — Wed, 29 Jul 2020 12:08:13 GMT

←Older revision		Revision as of 12:08, 29 July 2020
Line 1:		Line 1:

	==MSRECA-Q196E mutant==		==MSRECA-Q196E mutant==
-	<StructureSection load='2zr0' size='340' side='right' caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='2zr0' size='340' side='right'caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://pdbe.org/2zr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zr0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://pdbe.org/2zr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zr0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Deleted entry]]		[[Category: Deleted entry]]
		+	[[Category: Large Structures]]
	[[Category: Mycs2]]		[[Category: Mycs2]]
	[[Category: Chandra, N R]]		[[Category: Chandra, N R]]

OCA at 07:30, 18 October 2018

OCA — Thu, 18 Oct 2018 07:30:43 GMT

←Older revision		Revision as of 07:30, 18 October 2018
Line 1:		Line 1:
		+
	==MSRECA-Q196E mutant==		==MSRECA-Q196E mutant==
	<StructureSection load='2zr0' size='340' side='right' caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2zr0' size='340' side='right' caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://pdbe.org/2zr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://pdbe.org/2zr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zr0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zr/2zr0_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zr/2zr0_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 15:41, 7 February 2016

OCA — Sun, 07 Feb 2016 15:41:11 GMT

←Older revision		Revision as of 15:41, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='2zr0' size='340' side='right' caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='2zr0' size='340' side='right' caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://pdbe.org/2zr0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zr0 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zr0" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Deleted entry]]		[[Category: Deleted entry]]
-	[[Category: ~~Mycobacterium smegmatis str. mc2 155~~]]	+	[[Category: Mycs2]]
	[[Category: Chandra, N R]]		[[Category: Chandra, N R]]
	[[Category: Manjunath, G P]]		[[Category: Manjunath, G P]]

OCA at 16:23, 24 December 2014

OCA — Wed, 24 Dec 2014 16:23:07 GMT

←Older revision		Revision as of 16:23, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RECA_MYCS2 RECA_MYCS2]] Required for homologous recombination (HR) and the bypass of mutagenic DNA lesions (double strand breaks, DSB) by the SOS response. Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. Numerous X-ray crystals have been resolved under different conditions which indicate the flexibility of the protein, essential to its function. Gln-196 contributes to this plasticity by acting as a switch residue, which transmits the effect of nucleotide binding to the DNA-binding region.<ref>PMID:17360246</ref> <ref>PMID:17496093</ref> <ref>PMID:21219454</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Deleted entry]]		[[Category: Deleted entry]]
	[[Category: Mycobacterium smegmatis str. mc2 155]]		[[Category: Mycobacterium smegmatis str. mc2 155]]
-	[[Category: Chandra, N R.]]	+	[[Category: Chandra, N R]]
-	[[Category: Manjunath, G P.]]	+	[[Category: Manjunath, G P]]
-	[[Category: Muniyappa, K.]]	+	[[Category: Muniyappa, K]]
-	[[Category: Prabu, J R.]]	+	[[Category: Prabu, J R]]
-	[[Category: Vijayan, M.]]	+	[[Category: Vijayan, M]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
	[[Category: Dna damage]]		[[Category: Dna damage]]

OCA at 09:16, 29 September 2014

OCA — Mon, 29 Sep 2014 09:16:19 GMT

←Older revision		Revision as of 09:16, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~2zr0.~~png~~\|~~left~~\|200px]]	+	==MSRECA-Q196E mutant==
		+	<StructureSection load='2zr0' size='340' side='right' caption='[[2zr0]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZR0 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ubc\|1ubc]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deleted_entry Deleted entry], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.99.37 3.4.99.37] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/2zr0 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zr/2zr0_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structures of mutants of Mycobacterium smegmatis RecA (MsRecA) involving changes of Gln196 from glutamine to alanine, asparagine and glutamic acid, wild-type MsRecA and several of their nucleotide complexes have been determined using mostly low-temperature and partly room-temperature X-ray data. At both temperatures, nucleotide binding results in a movement of Gln196 towards the bound nucleotide in the wild-type protein. This movement is abolished in the mutants, thus establishing the structural basis for the triggering action of the residue in terms of the size, shape and the chemical nature of the side chain. The 19 crystal structures reported here, together with 11 previously reported MsRecA structures, provide further elaboration of the relation between the pitch of the ;inactive' RecA filament, the orientation of the C-terminal domain with respect to the main domain and the location of the switch residue. The low-temperature structures define one extreme of the range of positions the C-terminal domain can occupy. The movement of the C-terminal domain is correlated with those of the LexA-binding loop and the loop that connects the main and the N-terminal domains. These elements of molecular plasticity are made use of in the transition to the ;active' filament, as evidenced by the recently reported structures of RecA-DNA complexes. The available structures of RecA resulting from X-ray and electron-microscopic studies appear to represent different stages in the trajectory of the allosteric transformations of the RecA filament. The work reported here contributes to the description of the early stages of this trajectory and provides insight into structures relevant to the later stages.

-	~~{{STRUCTURE_2zr0\| PDB=2zr0 \| SCENE= }}~~	+	Functionally important movements in RecA molecules and filaments: studies involving mutation and environmental changes.,Prabu JR, Manjunath GP, Chandra NR, Muniyappa K, Vijayan M Acta Crystallogr D Biol Crystallogr. 2008 Nov;64(Pt 11):1146-57. Epub 2008, Oct 18. PMID:19020353<ref>PMID:19020353</ref>

-	~~===MSRECA-Q196E mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19020353}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[2zr0]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mycobacterium_smegmatis_str~~.~~_mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA].~~	+

	==See Also==		==See Also==
	*[[Recombinase A\|Recombinase A]]		*[[Recombinase A\|Recombinase A]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:019020353<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Deleted entry]]		[[Category: Deleted entry]]
	[[Category: Mycobacterium smegmatis str. mc2 155]]		[[Category: Mycobacterium smegmatis str. mc2 155]]

OCA at 07:06, 20 October 2012

OCA — Sat, 20 Oct 2012 07:06:04 GMT

←Older revision		Revision as of 07:06, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[2zr0]] is a 1 chain structure ~~of [[Recombinase A]]~~ with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA].	+	[[2zr0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA].

	==See Also==		==See Also==

OCA: Protected "2zr0" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 15:16:37 GMT

Protected "2zr0" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:16, 25 July 2012

OCA at 15:16, 25 July 2012

OCA — Wed, 25 Jul 2012 15:16:33 GMT

←Older revision		Revision as of 15:16, 25 July 2012
Line 1:		Line 1:
-	~~{{Seed}}~~	+	[[Image:2zr0.png\|left\|200px]]
-	[[Image:2zr0.~~jpg~~\|left\|200px]]	+

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2zr0", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2zr0\| PDB=2zr0 \| SCENE= }}		{{STRUCTURE_2zr0\| PDB=2zr0 \| SCENE= }}

	===MSRECA-Q196E mutant===		===MSRECA-Q196E mutant===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19020353}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19020353 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19020353}}		{{ABSTRACT_PUBMED_19020353}}

	==About this Structure==		==About this Structure==
-	~~2ZR0~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA].	+	[[2zr0]] is a 1 chain structure of [[Recombinase A]] with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis_str._mc2_155 Mycobacterium smegmatis str. mc2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZR0 OCA].
		+
		+	==See Also==
		+	*[[Recombinase A\|Recombinase A]]

	==Reference==		==Reference==
-	Functionally important movements in RecA molecules and filaments: studies involving mutation and environmental changes., Prabu JR, Manjunath GP, Chandra NR, Muniyappa K, Vijayan M, Acta Crystallogr D Biol Crystallogr. 2008 Nov;64(Pt 11):1146-57. Epub 2008, Oct 18. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~19020353 19020353]~~	+	<ref group="xtra">PMID:019020353</ref><references group="xtra"/>
	[[Category: Deleted entry]]		[[Category: Deleted entry]]
	[[Category: Mycobacterium smegmatis str. mc2 155]]		[[Category: Mycobacterium smegmatis str. mc2 155]]
Line 32:		Line 23:
	[[Category: Vijayan, M.]]		[[Category: Vijayan, M.]]
	[[Category: Atp-binding]]		[[Category: Atp-binding]]
-	[[Category: Cytoplasm]]
	[[Category: Dna damage]]		[[Category: Dna damage]]
	[[Category: Dna recombination]]		[[Category: Dna recombination]]
Line 43:		Line 33:
	[[Category: Recombination]]		[[Category: Recombination]]
	[[Category: Sos response]]		[[Category: Sos response]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 10 14:58:08 2008''