2zyl - Revision history

OCA at 13:59, 13 March 2024

OCA — Wed, 13 Mar 2024 13:59:01 GMT

←Older revision		Revision as of 13:59, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='2zyl' size='340' side='right'caption='[[2zyl]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2zyl' size='340' side='right'caption='[[2zyl]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus~~ tuberculosis~~" (zopf 1883) klein 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [~~http~~://pdbe.org/2zyl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/2zyl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=2zyl ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [https://pdbe.org/2zyl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [https://www.ebi.ac.uk/pdbsum/2zyl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zyl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KSHA_MYCTU KSHA_MYCTU]] Catalyzes the opening of ring B of 1,4-androstadiene-3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage.	+	[https://www.uniprot.org/uniprot/KSHA_MYCTU KSHA_MYCTU] Catalyzes the opening of ring B of 1,4-androstadiene-3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zyl ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zyl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	KshAB (3-Ketosteroid 9alpha-hydroxylase) is a two-component Rieske oxygenase (RO) in the cholesterol catabolic pathway of Mycobacterium tuberculosis. Although the enzyme has been implicated in pathogenesis, it has largely been characterized by bioinformatics and molecular genetics. Purified KshB, the reductase component, was a monomeric protein containing a plant-type [2Fe-2S] cluster and FAD. KshA, the oxygenase, was a homotrimer containing a Rieske [2Fe-2S] cluster and mononuclear ferrous iron. Of two potential substrates, reconstituted KshAB had twice the specificity for 1,4-androstadiene-3,17-dione as for 4-androstene-3,17-dione. The transformation of both substrates was well coupled to the consumption of O(2). Nevertheless, the reactivity of KshAB with O(2) was low in the presence of 1,4-androstadiene-3,17-dione, with a k(cat)/K(m)(O(2)) of 2450 +/- 80 m(-1) s(-1). The crystallographic structure of KshA, determined to 2.3A(,) revealed an overall fold and a head-to-tail subunit arrangement typical of ROs. The central fold of the catalytic domain lacks all insertions found in characterized ROs, consistent with a minimal and perhaps archetypical RO catalytic domain. The structure of KshA is further distinguished by a C-terminal helix, which stabilizes subunit interactions in the functional trimer. Finally, the substrate-binding pocket extends farther into KshA than in other ROs, consistent with the large steroid substrate, and the funnel accessing the active site is differently orientated. This study provides a solid basis for further studies of a key steroid-transforming enzyme of biotechnological and medical importance.
-
-	Characterization of 3-ketosteroid 9{alpha}-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis.,Capyk JK, D'Angelo I, Strynadka NC, Eltis LD J Biol Chem. 2009 Apr 10;284(15):9937-46. Epub 2009 Feb 20. PMID:19234303<ref>PMID:19234303</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 2zyl" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Angelo, I D~~]]	+	[[Category: Mycobacterium tuberculosis]]
-	[[Category: Capyk, J]]	+	[[Category: Capyk J]]
-	[[Category: ~~Eltis, L~~]]	+	[[Category: D'Angelo I]]
-	[[Category: ~~Strynadka, N~~]]	+	[[Category: Eltis L]]
-	[[Category: ~~Cholesterol]]~~	+	[[Category: Strynadka N]]
-	~~[[Category: Ksha]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rieske~~]]	+

OCA at 12:11, 29 July 2020

OCA — Wed, 29 Jul 2020 12:11:49 GMT

←Older revision		Revision as of 12:11, 29 July 2020
Line 1:		Line 1:

	==Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis==		==Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis==
-	<StructureSection load='2zyl' size='340' side='right' caption='[[2zyl]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='2zyl' size='340' side='right'caption='[[2zyl]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [http://pdbe.org/2zyl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [http://www.ebi.ac.uk/pdbsum/2zyl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zyl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [http://pdbe.org/2zyl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [http://www.ebi.ac.uk/pdbsum/2zyl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zyl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Angelo, I D]]		[[Category: Angelo, I D]]
	[[Category: Capyk, J]]		[[Category: Capyk, J]]

OCA at 07:25, 18 October 2018

OCA — Thu, 18 Oct 2018 07:25:49 GMT

←Older revision		Revision as of 07:25, 18 October 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/2zyl_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/2zyl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 07:32, 5 August 2016

OCA — Fri, 05 Aug 2016 07:32:58 GMT

←Older revision		Revision as of 07:32, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis==		==Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis==
	<StructureSection load='2zyl' size='340' side='right' caption='[[2zyl]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='2zyl' size='340' side='right' caption='[[2zyl]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mycobacterium_tuberculosis Mycobacterium~~ tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [http://www.ebi.ac.uk/pdbsum/2zyl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [http://pdbe.org/2zyl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [http://www.ebi.ac.uk/pdbsum/2zyl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zyl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~P71875_MYCTU P71875_MYCTU~~]] Catalyzes the opening of ring B of 1,4-androstadiene-3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage.	+	[[http://www.uniprot.org/uniprot/KSHA_MYCTU KSHA_MYCTU]] Catalyzes the opening of ring B of 1,4-androstadiene-3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zyl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 2zyl" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Mycobacterium tuberculosis]]
	[[Category: Angelo, I D]]		[[Category: Angelo, I D]]
	[[Category: Capyk, J]]		[[Category: Capyk, J]]

OCA at 16:55, 25 December 2014

OCA — Thu, 25 Dec 2014 16:55:45 GMT

←Older revision		Revision as of 16:55, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>		<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [http://www.ebi.ac.uk/pdbsum/2zyl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [http://www.ebi.ac.uk/pdbsum/2zyl PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/P71875_MYCTU P71875_MYCTU]] Catalyzes the opening of ring B of 1,4-androstadiene-3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
-	[[Category: Angelo, I D.]]	+	[[Category: Angelo, I D]]
-	[[Category: Capyk, J.]]	+	[[Category: Capyk, J]]
-	[[Category: Eltis, L.]]	+	[[Category: Eltis, L]]
-	[[Category: Strynadka, N.]]	+	[[Category: Strynadka, N]]
	[[Category: Cholesterol]]		[[Category: Cholesterol]]
	[[Category: Ksha]]		[[Category: Ksha]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rieske]]		[[Category: Rieske]]

OCA at 05:36, 2 October 2014

OCA — Thu, 02 Oct 2014 05:36:01 GMT

←Older revision		Revision as of 05:36, 2 October 2014
Line 1:		Line 1:
-	[[~~Image~~:2zyl.~~png\|left~~\|200px]]	+	==Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis==
		+	<StructureSection load='2zyl' size='340' side='right' caption='[[2zyl]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[2zyl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZYL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zyl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zyl RCSB], [http://www.ebi.ac.uk/pdbsum/2zyl PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zy/2zyl_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	KshAB (3-Ketosteroid 9alpha-hydroxylase) is a two-component Rieske oxygenase (RO) in the cholesterol catabolic pathway of Mycobacterium tuberculosis. Although the enzyme has been implicated in pathogenesis, it has largely been characterized by bioinformatics and molecular genetics. Purified KshB, the reductase component, was a monomeric protein containing a plant-type [2Fe-2S] cluster and FAD. KshA, the oxygenase, was a homotrimer containing a Rieske [2Fe-2S] cluster and mononuclear ferrous iron. Of two potential substrates, reconstituted KshAB had twice the specificity for 1,4-androstadiene-3,17-dione as for 4-androstene-3,17-dione. The transformation of both substrates was well coupled to the consumption of O(2). Nevertheless, the reactivity of KshAB with O(2) was low in the presence of 1,4-androstadiene-3,17-dione, with a k(cat)/K(m)(O(2)) of 2450 +/- 80 m(-1) s(-1). The crystallographic structure of KshA, determined to 2.3A(,) revealed an overall fold and a head-to-tail subunit arrangement typical of ROs. The central fold of the catalytic domain lacks all insertions found in characterized ROs, consistent with a minimal and perhaps archetypical RO catalytic domain. The structure of KshA is further distinguished by a C-terminal helix, which stabilizes subunit interactions in the functional trimer. Finally, the substrate-binding pocket extends farther into KshA than in other ROs, consistent with the large steroid substrate, and the funnel accessing the active site is differently orientated. This study provides a solid basis for further studies of a key steroid-transforming enzyme of biotechnological and medical importance.

-	{~~{STRUCTURE_2zyl\| PDB=2zyl \| SCENE= }~~}	+	Characterization of 3-ketosteroid 9{alpha}-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis.,Capyk JK, D'Angelo I, Strynadka NC, Eltis LD J Biol Chem. 2009 Apr 10;284(15):9937-46. Epub 2009 Feb 20. PMID:19234303<ref>PMID:19234303</ref>

-	~~===Crystal structure~~ of ~~3-ketosteroid-9-alpha-hydroxylase (KshA) from M~~. ~~tuberculosis===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_19234303}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[2zyl]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:019234303<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Angelo, I D.]]		[[Category: Angelo, I D.]]

OCA: Protected "2zyl" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 20:22:54 GMT

Protected "2zyl" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:22, 7 January 2013

OCA at 20:22, 7 January 2013

OCA — Mon, 07 Jan 2013 20:22:52 GMT

←Older revision		Revision as of 20:22, 7 January 2013
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-	{{Seed}}
	[[Image:2zyl.png\|left\|200px]]		[[Image:2zyl.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_2zyl", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_2zyl\| PDB=2zyl \| SCENE= }}		{{STRUCTURE_2zyl\| PDB=2zyl \| SCENE= }}

	===Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis===		===Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_19234303}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 19234303 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_19234303}}		{{ABSTRACT_PUBMED_19234303}}

	==About this Structure==		==About this Structure==
-	~~2ZYL~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA].	+	[[2zyl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~19234303~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:019234303</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Angelo, I D.]]		[[Category: Angelo, I D.]]
Line 33:		Line 21:
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rieske]]		[[Category: Rieske]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 3 09:25:00 2009''

OCA at 06:25, 3 June 2009

OCA — Wed, 03 Jun 2009 06:25:01 GMT

←Older revision		Revision as of 06:25, 3 June 2009
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:2zyl.~~jpg~~\|left\|200px]]	+	[[Image:2zyl.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis===		===Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_19234303}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 19234303 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_19234303}}

	==About this Structure==		==About this Structure==
	2ZYL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA].		2ZYL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:19234303</ref><references group="xtra"/>
	[[Category: Mycobacterium tuberculosis]]		[[Category: Mycobacterium tuberculosis]]
	[[Category: Angelo, I D.]]		[[Category: Angelo, I D.]]
Line 25:		Line 34:
	[[Category: Rieske]]		[[Category: Rieske]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Mar~~ ~~4 14~~:39:39 2009''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 3 09:25:00 2009''

OCA at 12:39, 4 March 2009

OCA — Wed, 04 Mar 2009 12:39:42 GMT

←Older revision		Revision as of 12:39, 4 March 2009
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	{{Seed}}
		+	[[Image:2zyl.jpg\|left\|200px]]

-	The ~~entry 2zyl~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_2zyl", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_2zyl\| PDB=2zyl \| SCENE= }}

-	~~Authors: D'Angelo, I, Capyk, J., Strynadka, N., Eltis, L~~.	+	===Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis===

-	Description: Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (KshA) from M. tuberculosis

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Feb 18 08~~:52:29 2009''	+	==About this Structure==
		+	2ZYL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZYL OCA].
		+	[[Category: Mycobacterium tuberculosis]]
		+	[[Category: Angelo, I D.]]
		+	[[Category: Capyk, J.]]
		+	[[Category: Eltis, L.]]
		+	[[Category: Strynadka, N.]]
		+	[[Category: Cholesterol]]
		+	[[Category: Ksha]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rieske]]
		+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 4 14:39:39 2009''