314d - Revision history

OCA at 08:38, 7 February 2024

OCA — Wed, 07 Feb 2024 08:38:54 GMT

←Older revision		Revision as of 08:38, 7 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=314D FirstGlance]. <br>		<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=314D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [https://pdbe.org/314d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [https://www.ebi.ac.uk/pdbsum/314d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=314d ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [https://pdbe.org/314d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [https://www.ebi.ac.uk/pdbsum/314d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=314d ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	We have solved the structures of the homoduplex d(Gm5CGCGCG)2, and the heteroduplexes d(GCGCGCG)/d(TCGCGCG) and d(GCGCGCG)/d(CCGCGCG). The structures form six base-pairs of identical Z-DNA duplexes with single nucleotides overhanging at the 5'-ends. The overhanging nucleotide from one strand remains stacked and sandwiched between the blunt-ends of two adjacent Z-DNA duplexes, while the overhanging base of the opposing strand is extra-helical. The stacked and the extra-helical bases from adjacent duplexes pair to form a distorted d(G x G) reverse Hoogsteen base-pair in the d(Gm5CGCGCG)2 homoduplex, and d(G x T) reverse wobble and d(G x C) reverse Watson-Crick base-pairs in the d(GCGCGCG)/d(TCGCGCG) and d(GCGCGCG)/d(CCGCGCG) heteroduplexes, respectively. Interestingly, only the d(G,T) and d(G x C) base-pairs were observed in the heteroduplexes, suggesting that both the d(G x T) reverse wobble and d(G x C) reverse Watson-Crick base-pairs are more stable in this crystal environment than the d(G x G) reverse Hoogsteen base-pair. To estimate the relative stability of the three types of reverse base-pairs, crystals were grown using various mixtures of sequences and their strand compositions analyzed by mass spectrometry. The d(G x C) reverse Watson-Crick base-pair was estimated to be more stable by approximately 1.5 kcal/mol and the d(G x T) reverse wobble base-pair more stable by approximately 0.5 kcal/mol than the d(G x G) reverse Hoogsteen base-pair. The step during crystallization responsible for discriminating between the strands in the crystal is highly cooperative, suggesting that it occurs during the initial nucleating event of crystal growth.
-
-	The structures and relative stabilities of d(G x G) reverse Hoogsteen, d(G x T) reverse wobble, and d(G x C) reverse Watson-Crick base-pairs in DNA crystals.,Mooers BH, Eichman BF, Ho PS J Mol Biol. 1997 Jun 27;269(5):796-810. PMID:9223642<ref>PMID:9223642</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 314d" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Z-DNA\|Z-DNA]]		*[[Z-DNA\|Z-DNA]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eichman~~, B F~~]]	+	[[Category: Eichman BF]]
-	[[Category: Ho~~, P S~~]]	+	[[Category: Ho PS]]
-	[[Category: Mooers~~, B H.M]]~~	+	[[Category: Mooers BHM]]
-	~~[[Category: Dna]]~~	+
-	~~[[Category: Double helix]]~~	+
-	~~[[Category: Flipped-out base]]~~	+
-	~~[[Category: Overhanging base]]~~	+
-	~~[[Category: Z-dna~~]]	+

OCA at 13:23, 4 May 2022

OCA — Wed, 04 May 2022 13:23:31 GMT

←Older revision		Revision as of 13:23, 4 May 2022
Line 3:		Line 3:
	<StructureSection load='314d' size='340' side='right'caption='[[314d]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='314d' size='340' side='right'caption='[[314d]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=314D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=314D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [~~http~~://pdbe.org/314d PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/314d PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=314d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [https://pdbe.org/314d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [https://www.ebi.ac.uk/pdbsum/314d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=314d ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:12, 29 July 2020

OCA — Wed, 29 Jul 2020 12:12:59 GMT

←Older revision		Revision as of 12:12, 29 July 2020
Line 1:		Line 1:

	==Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR==		==Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR==
-	<StructureSection load='314d' size='340' side='right' caption='[[314d]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='314d' size='340' side='right'caption='[[314d]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=314D FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=314D FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [http://pdbe.org/314d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [http://www.ebi.ac.uk/pdbsum/314d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=314d ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [http://pdbe.org/314d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [http://www.ebi.ac.uk/pdbsum/314d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=314d ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Eichman, B F]]		[[Category: Eichman, B F]]
	[[Category: Ho, P S]]		[[Category: Ho, P S]]

OCA at 06:57, 18 October 2018

OCA — Thu, 18 Oct 2018 06:57:43 GMT

←Older revision		Revision as of 06:57, 18 October 2018
Line 16:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 314d" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 314d" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Z-DNA\|Z-DNA]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:01, 11 August 2016

OCA — Thu, 11 Aug 2016 16:01:49 GMT

←Older revision		Revision as of 16:01, 11 August 2016
Line 1:		Line 1:
		+
	==Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR==		==Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR==
	<StructureSection load='314d' size='340' side='right' caption='[[314d]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='314d' size='340' side='right' caption='[[314d]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=314D FirstGlance]. <br>		<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=314D FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [http://www.ebi.ac.uk/pdbsum/314d PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [http://pdbe.org/314d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [http://www.ebi.ac.uk/pdbsum/314d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=314d ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 15:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 314d" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:16, 25 January 2015

OCA — Sun, 25 Jan 2015 10:16:50 GMT

←Older revision		Revision as of 10:16, 25 January 2015
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Eichman, B F.]]	+	[[Category: Eichman, B F]]
-	[[Category: Ho, P S.]]	+	[[Category: Ho, P S]]
-	[[Category: Mooers, B H.M.]]	+	[[Category: Mooers, B H.M]]
	[[Category: Dna]]		[[Category: Dna]]
	[[Category: Double helix]]		[[Category: Double helix]]

OCA at 11:55, 29 October 2014

OCA — Wed, 29 Oct 2014 11:55:57 GMT

←Older revision		Revision as of 11:55, 29 October 2014
Line 1:		Line 1:
-	[[~~Image:~~314d.~~png~~\|~~left\|200px~~]]	+	==Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR==
		+	<StructureSection load='314d' size='340' side='right' caption='[[314d]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=314D FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=314d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=314d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=314d RCSB], [http://www.ebi.ac.uk/pdbsum/314d PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have solved the structures of the homoduplex d(Gm5CGCGCG)2, and the heteroduplexes d(GCGCGCG)/d(TCGCGCG) and d(GCGCGCG)/d(CCGCGCG). The structures form six base-pairs of identical Z-DNA duplexes with single nucleotides overhanging at the 5'-ends. The overhanging nucleotide from one strand remains stacked and sandwiched between the blunt-ends of two adjacent Z-DNA duplexes, while the overhanging base of the opposing strand is extra-helical. The stacked and the extra-helical bases from adjacent duplexes pair to form a distorted d(G x G) reverse Hoogsteen base-pair in the d(Gm5CGCGCG)2 homoduplex, and d(G x T) reverse wobble and d(G x C) reverse Watson-Crick base-pairs in the d(GCGCGCG)/d(TCGCGCG) and d(GCGCGCG)/d(CCGCGCG) heteroduplexes, respectively. Interestingly, only the d(G,T) and d(G x C) base-pairs were observed in the heteroduplexes, suggesting that both the d(G x T) reverse wobble and d(G x C) reverse Watson-Crick base-pairs are more stable in this crystal environment than the d(G x G) reverse Hoogsteen base-pair. To estimate the relative stability of the three types of reverse base-pairs, crystals were grown using various mixtures of sequences and their strand compositions analyzed by mass spectrometry. The d(G x C) reverse Watson-Crick base-pair was estimated to be more stable by approximately 1.5 kcal/mol and the d(G x T) reverse wobble base-pair more stable by approximately 0.5 kcal/mol than the d(G x G) reverse Hoogsteen base-pair. The step during crystallization responsible for discriminating between the strands in the crystal is highly cooperative, suggesting that it occurs during the initial nucleating event of crystal growth.

-	~~{{STRUCTURE_314d\| PDB=314d \| SCENE= }}~~	+	The structures and relative stabilities of d(G x G) reverse Hoogsteen, d(G x T) reverse wobble, and d(G x C) reverse Watson-Crick base-pairs in DNA crystals.,Mooers BH, Eichman BF, Ho PS J Mol Biol. 1997 Jun 27;269(5):796-810. PMID:9223642<ref>PMID:9223642</ref>

-	~~===Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9223642}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[314d]] is a 2 chain structure. Full crystallographic information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA].~~	+	</StructureSection>
	[[Category: Eichman, B F.]]		[[Category: Eichman, B F.]]
	[[Category: Ho, P S.]]		[[Category: Ho, P S.]]

OCA: Protected "314d" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 19:18:56 GMT

Protected "314d" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:18, 7 January 2013

OCA at 19:18, 7 January 2013

OCA — Mon, 07 Jan 2013 19:18:54 GMT

←Older revision		Revision as of 19:18, 7 January 2013
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-	{{Seed}}
	[[Image:314d.png\|left\|200px]]		[[Image:314d.png\|left\|200px]]

-	<!--
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-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_314d\| PDB=314d \| SCENE= }}		{{STRUCTURE_314d\| PDB=314d \| SCENE= }}

	===Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR===		===Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9223642}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9223642 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_9223642}}		{{ABSTRACT_PUBMED_9223642}}

	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA].	+	[[314d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=314D OCA].
-		+
-	~~==Reference==~~	+
-	The structures and relative stabilities of d(G x G) reverse Hoogsteen, d(G x T) reverse wobble, and d(G x C) reverse Watson-Crick base-pairs in DNA crystals., Mooers BH, Eichman BF, Ho PS, J Mol Biol. 1997 Jun 27;269(5):796-810. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9223642 9223642]	+
	[[Category: Eichman, B F.]]		[[Category: Eichman, B F.]]
	[[Category: Ho, P S.]]		[[Category: Ho, P S.]]
	[[Category: Mooers, B H.M.]]		[[Category: Mooers, B H.M.]]
		+	[[Category: Dna]]
	[[Category: Double helix]]		[[Category: Double helix]]
	[[Category: Flipped-out base]]		[[Category: Flipped-out base]]
	[[Category: Overhanging base]]		[[Category: Overhanging base]]
	[[Category: Z-dna]]		[[Category: Z-dna]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:50:08 2008''

OCA at 18:50, 28 July 2008

OCA — Mon, 28 Jul 2008 18:50:15 GMT

←Older revision		Revision as of 18:50, 28 July 2008
Line 1:		Line 1:
-	[[Image:314d.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:314d.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_314d\| PDB=314d \| SCENE= }}		{{STRUCTURE_314d\| PDB=314d \| SCENE= }}

-	~~'''~~Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR~~'''~~	+	===Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR===


-	~~==Overview==~~	+	<!--
-	~~We have solved the structures of the homoduplex d(Gm5CGCGCG)2, and the heteroduplexes d(GCGCGCG)/d(TCGCGCG) and d(GCGCGCG)/d(CCGCGCG). The structures form six base~~-~~pairs of identical Z~~-~~DNA duplexes with single nucleotides overhanging at the 5'-ends.~~ The ~~overhanging nucleotide from one strand remains stacked and sandwiched between the blunt-ends of two adjacent Z-DNA duplexes~~, ~~while~~ the ~~overhanging base of the opposing strand is extra-helical. The stacked and the extra-helical bases from adjacent duplexes pair~~ to ~~form a distorted d(G x G) reverse Hoogsteen base-pair in~~ the d(~~Gm5CGCGCG)2 homoduplex, and d(G x T) reverse wobble and d(G x C) reverse Watson-Crick base-pairs in the d(GCGCGCG)~~/~~d(TCGCGCG) and d(GCGCGCG)~~/~~d(CCGCGCG) heteroduplexes, respectively~~. ~~Interestingly, only the d(G,T~~) ~~and d(G x C) base-pairs were observed in the heteroduplexes~~, ~~suggesting that both~~ the ~~d(G x T) reverse wobble and d(G x C) reverse Watson-Crick base-pairs are more stable in this crystal environment than the d(G x G) reverse Hoogsteen base-pair~~. ~~To estimate the relative stability of the three types of reverse base~~-~~pairs, crystals were grown using various mixtures of sequences and their strand compositions analyzed by mass spectrometry. The d(G x C) reverse Watson~~-Crick base-pair was estimated to be more stable by approximately 1.5 kcal/mol and the d(G x T) reverse wobble base-pair more stable by approximately 0.5 kcal/mol than the d(G x G) reverse Hoogsteen base-pair. The step during crystallization responsible for discriminating between the strands in the crystal is highly cooperative, suggesting that it occurs during the initial nucleating event of crystal growth.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9223642}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9223642 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9223642}}

	==About this Structure==		==About this Structure==
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	[[Category: Overhanging base]]		[[Category: Overhanging base]]
	[[Category: Z-dna]]		[[Category: Z-dna]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 20~~:15:18 2008''	+
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