32c2 - Revision history

OCA at 12:00, 30 August 2023

OCA — Wed, 30 Aug 2023 12:00:24 GMT

←Older revision		Revision as of 12:00, 30 August 2023
Line 3:		Line 3:
	<StructureSection load='32c2' size='340' side='right'caption='[[32c2]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='32c2' size='340' side='right'caption='[[32c2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=32C2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=32C2 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [~~http~~://pdbe.org/32c2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/32c2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=32c2 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [https://pdbe.org/32c2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [https://www.ebi.ac.uk/pdbsum/32c2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=32c2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 34:		Line 35:
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Burkhart, B]]	+	[[Category: Burkhart B]]
-	[[Category: Ghosh, D]]	+	[[Category: Ghosh D]]
-	[[Category: Higashiyama, T]]	+	[[Category: Higashiyama T]]
-	[[Category: Ng~~, P C~~]]	+	[[Category: Ng PC]]
-	[[Category: Osawa, Y]]	+	[[Category: Osawa Y]]
-	[[Category: Pletnev, V]]	+	[[Category: Pletnev V]]
-	[[Category: Sawicki~~, M W]]~~	+	[[Category: Sawicki MW]]
-	~~[[Category: Antibody]]~~	+
-	~~[[Category: Aromatase]]~~	+
-	~~[[Category: Fab]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: P450~~]]	+

OCA at 12:13, 29 July 2020

OCA — Wed, 29 Jul 2020 12:13:32 GMT

←Older revision		Revision as of 12:13, 29 July 2020
Line 1:		Line 1:

	==STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE==		==STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE==
-	<StructureSection load='32c2' size='340' side='right' caption='[[32c2]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='32c2' size='340' side='right'caption='[[32c2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=32C2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=32C2 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [http://pdbe.org/32c2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [http://www.ebi.ac.uk/pdbsum/32c2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=32c2 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [http://pdbe.org/32c2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [http://www.ebi.ac.uk/pdbsum/32c2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=32c2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Burkhart, B]]		[[Category: Burkhart, B]]

OCA at 07:18, 18 October 2018

OCA — Thu, 18 Oct 2018 07:18:58 GMT

←Older revision		Revision as of 07:18, 18 October 2018
Line 10:		Line 10:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/2c/32c2_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/2c/32c2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:

	==See Also==		==See Also==
-	*[[3D structures ~~of monoclonal antibody~~\|3D structures ~~of monoclonal antibody~~]]	+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:40, 11 August 2016

OCA — Thu, 11 Aug 2016 14:40:26 GMT

←Older revision		Revision as of 14:40, 11 August 2016
Line 1:		Line 1:
		+
	==STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE==		==STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE==
	<StructureSection load='32c2' size='340' side='right' caption='[[32c2]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='32c2' size='340' side='right' caption='[[32c2]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32C2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32C2 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [http://www.ebi.ac.uk/pdbsum/32c2 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [http://pdbe.org/32c2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [http://www.ebi.ac.uk/pdbsum/32c2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=32c2 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=32c2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 32c2" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[~~Monoclonal Antibody~~\|~~Monoclonal Antibody~~]]	+	*[[3D structures of monoclonal antibody\|3D structures of monoclonal antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:20, 20 January 2015

OCA — Tue, 20 Jan 2015 08:20:49 GMT

←Older revision		Revision as of 08:20, 20 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32C2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32C2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [http://www.ebi.ac.uk/pdbsum/32c2 PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [http://www.ebi.ac.uk/pdbsum/32c2 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Burkhart, B.]]	+	[[Category: Burkhart, B]]
-	[[Category: Ghosh, D.]]	+	[[Category: Ghosh, D]]
-	[[Category: Higashiyama, T.]]	+	[[Category: Higashiyama, T]]
-	[[Category: Ng, P C.]]	+	[[Category: Ng, P C]]
-	[[Category: Osawa, Y.]]	+	[[Category: Osawa, Y]]
-	[[Category: Pletnev, V.]]	+	[[Category: Pletnev, V]]
-	[[Category: Sawicki, M W.]]	+	[[Category: Sawicki, M W]]
	[[Category: Antibody]]		[[Category: Antibody]]
	[[Category: Aromatase]]		[[Category: Aromatase]]

OCA at 05:01, 2 October 2014

OCA — Thu, 02 Oct 2014 05:01:21 GMT

←Older revision		Revision as of 05:01, 2 October 2014
Line 1:		Line 1:
-	[[~~Image~~:32c2.~~png\|left~~\|200px]]	+	==STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE==
		+	<StructureSection load='32c2' size='340' side='right' caption='[[32c2]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[32c2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32C2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=32c2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=32c2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=32c2 RCSB], [http://www.ebi.ac.uk/pdbsum/32c2 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/2c/32c2_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of a Fab fragment (Fab3-2C2) of a monoclonal antibody raised against aromatase cytochrome P450 P450arom) has been determined at 3.0 A resolution. P450arom is a membrane bound enzyme responsible for the catalysis of indrogens to estrogens, the process of aromatization, and hence has been implicated in hormone-dependent breast cancer. The Fab fragment of MAb3-2C2 IgG suppresses P450arom activity in a dose dependent manner. The Fab3-2C2 molecule crystallizes n the space group P2(1)2(1)2(1) with a unit cell of a= 154.89 A, b = 73.51 A, and c= 36.90 A. The crystal structure consists of a light and a heavy chain in the asymmetric unit, each characterized by the greek-key antiparallel beta barrel folding seen in all Fab structures. The average elbow angle between the two domains is 143 degrees. Modeling of the interactions between the variable domains of the antibody and a known model of P450arom maps the epitope to a region of the enzyme that is consistent with the available biochemical data and the activity-suppressing function of the antibody. The epitope mapping result is further supported by the inability of MAb3-2C2 IgG to suppress the activity of, or to interact with placental porcine P450arom, which is 81% identical (86% similar) to human P450arom but has a few key substitutions in the putative epitope region.

-	~~{{STRUCTURE_32c2\| PDB=32c2 \| SCENE= }}~~	+	Structure of an activity suppressing Fab fragment to cytochrome P450 aromatase: insights into the antibody-antigen interactions.,Sawicki MW, Ng PC, Burkhart BM, Pletnev VZ, Higashiyama T, Osawa Y, Ghosh D Mol Immunol. 1999 May;36(7):423-32. PMID:10449095<ref>PMID:10449095</ref>

-	~~===STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_10449095}}~~	+	==See Also==
-		+	*[[Monoclonal Antibody\|Monoclonal Antibody]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~32c2~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:010449095<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Burkhart, B.]]		[[Category: Burkhart, B.]]

OCA: Protected "32c2" [edit=sysop:move=sysop]

OCA — Mon, 07 Jan 2013 18:11:42 GMT

Protected "32c2" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:11, 7 January 2013

OCA at 18:11, 7 January 2013

OCA — Mon, 07 Jan 2013 18:11:38 GMT

←Older revision		Revision as of 18:11, 7 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:32c2.png\|left\|200px]]		[[Image:32c2.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_32c2", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_32c2\| PDB=32c2 \| SCENE= }}		{{STRUCTURE_32c2\| PDB=32c2 \| SCENE= }}

	===STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE===		===STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10449095}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10449095 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10449095}}		{{ABSTRACT_PUBMED_10449095}}

	==About this Structure==		==About this Structure==
-	~~32C2~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA].	+	[[32c2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10449095~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010449095</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Burkhart, B.]]		[[Category: Burkhart, B.]]
Line 35:		Line 23:
	[[Category: Aromatase]]		[[Category: Aromatase]]
	[[Category: Fab]]		[[Category: Fab]]
		+	[[Category: Immune system]]
	[[Category: P450]]		[[Category: P450]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:14:22 2009''

OCA at 02:14, 18 February 2009

OCA — Wed, 18 Feb 2009 02:14:23 GMT

←Older revision		Revision as of 02:14, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA].	+	32C2 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=32C2 OCA].

	==Reference==		==Reference==
-	Structure of an activity suppressing Fab fragment to cytochrome P450 aromatase: insights into the antibody-antigen interactions., Sawicki MW, Ng PC, Burkhart BM, Pletnev VZ, Higashiyama T, Osawa Y, Ghosh D, Mol Immunol. 1999 May;36(7):423-32. PMID:[~~http~~:~~//www.ncbi.nlm.nih.gov/pubmed/10449095 10449095~~]	+	<ref group="xtra">PMID:10449095</ref><references group="xtra"/>
		+	[[Category: Mus musculus]]
	[[Category: Burkhart, B.]]		[[Category: Burkhart, B.]]
	[[Category: Ghosh, D.]]		[[Category: Ghosh, D.]]
Line 36:		Line 37:
	[[Category: P450]]		[[Category: P450]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 10~~:49:~~43 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:14:22 2009''

OCA at 07:49, 29 July 2008

OCA — Tue, 29 Jul 2008 07:49:55 GMT

←Older revision		Revision as of 07:49, 29 July 2008
Line 1:		Line 1:
-	[[Image:32c2.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:32c2.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_32c2\| PDB=32c2 \| SCENE= }}		{{STRUCTURE_32c2\| PDB=32c2 \| SCENE= }}

-	~~'''~~STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE~~'''~~	+	===STRUCTURE OF AN ACTIVITY SUPPRESSING FAB FRAGMENT TO CYTOCHROME P450 AROMATASE===


-	~~==Overview==~~	+	<!--
-	The crystal structure of a Fab fragment (Fab3-2C2) of a monoclonal antibody raised against aromatase cytochrome P450 P450arom) has been determined at 3.0 A resolution. P450arom is a membrane bound enzyme responsible for the ~~catalysis of indrogens~~ to ~~estrogens,~~ the ~~process of aromatization, and hence has been implicated in hormone-dependent breast cancer~~. ~~The Fab fragment of MAb3-2C2 IgG suppresses P450arom activity in a dose dependent manner~~. ~~The Fab3-2C2 molecule crystallizes n the space group P2(1~~)~~2(1)2(1) with a unit cell of a= 154.89 A~~, b = 73.51 A, and c= 36.90 A. The crystal structure consists of a light and a heavy chain in the asymmetric unit, each characterized by the greek-key antiparallel beta barrel folding seen in all Fab structures. The average elbow angle between the two domains is ~~143 degrees. Modeling of~~ the interactions between the variable domains of the antibody and a known model of P450arom maps the epitope to a region of the enzyme that is consistent with the available biochemical data and the activity-suppressing function of the antibody. ~~The epitope mapping result is further supported by the inability of MAb3~~-2C2 IgG to suppress the activity of, or to interact with placental porcine P450arom, which is 81% identical (86% similar) to human P450arom but has a few key substitutions in the putative epitope region.	+	The line below this paragraph, {{ABSTRACT_PUBMED_10449095}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 10449095 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_10449095}}

	==About this Structure==		==About this Structure==
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	[[Category: Fab]]		[[Category: Fab]]
	[[Category: P450]]		[[Category: P450]]
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