3a3p - Revision history

OCA at 14:09, 1 November 2023

OCA — Wed, 01 Nov 2023 14:09:42 GMT

←Older revision		Revision as of 14:09, 1 November 2023
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	<StructureSection load='3a3p' size='340' side='right'caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3a3p' size='340' side='right'caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A3P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis Thermococcus kodakarensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A3P FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[2z30\|2z30]]~~, ~~[[3a3n\|3a3n]], [[3a3o\|3a3o]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [https://pdbe.org/3a3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [https://www.ebi.ac.uk/pdbsum/3a3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a3p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [https://pdbe.org/3a3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [https://www.ebi.ac.uk/pdbsum/3a3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a3p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TKSU_THEKO TKSU_THEKO]] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.	+	[https://www.uniprot.org/uniprot/TKSU_THEKO TKSU_THEKO] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Thermococcus kodakaraensis atomi et al. 2004]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kanaya, S]]	+	[[Category: Thermococcus kodakarensis]]
-	[[Category: Koga, Y]]	+	[[Category: Kanaya S]]
-	[[Category: Matsumura, H]]	+	[[Category: Koga Y]]
-	[[Category: Takano, K]]	+	[[Category: Matsumura H]]
-	[[Category: Tanaka, S]]	+	[[Category: Takano K]]
-	~~[[Category: Hydrolase]]~~	+	[[Category: Tanaka S]]
-	~~[[Category: Propeptide]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Serine protease]]~~	+
-	~~[[Category: Subtilisin]]~~	+
-	~~[[Category: Thermococcus kodakaraensis]]~~	+
-	~~[[Category: Zymogen~~]]	+

OCA at 07:22, 10 November 2021

OCA — Wed, 10 Nov 2021 07:22:39 GMT

←Older revision		Revision as of 07:22, 10 November 2021
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	<StructureSection load='3a3p' size='340' side='right'caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3a3p' size='340' side='right'caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=3A3P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A3P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [~~http~~://pdbe.org/3a3p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3a3p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3a3p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [https://pdbe.org/3a3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [https://www.ebi.ac.uk/pdbsum/3a3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a3p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TKSU_THEKO TKSU_THEKO]] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.	+	[[https://www.uniprot.org/uniprot/TKSU_THEKO TKSU_THEKO]] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:16, 29 July 2020

OCA — Wed, 29 Jul 2020 12:16:13 GMT

←Older revision		Revision as of 12:16, 29 July 2020
Line 1:		Line 1:

	==Crystal structure of complex between E201A/SA-subtilisin and Tk-propeptide==		==Crystal structure of complex between E201A/SA-subtilisin and Tk-propeptide==
-	<StructureSection load='3a3p' size='340' side='right' caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3a3p' size='340' side='right'caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3A3P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3A3P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://pdbe.org/3a3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3a3p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://pdbe.org/3a3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3a3p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Subtilisin\|Subtilisin]]	+	*[[Subtilisin 3D structures\|Subtilisin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Thermococcus kodakaraensis atomi et al. 2004]]		[[Category: Thermococcus kodakaraensis atomi et al. 2004]]
		+	[[Category: Large Structures]]
	[[Category: Kanaya, S]]		[[Category: Kanaya, S]]
	[[Category: Koga, Y]]		[[Category: Koga, Y]]

OCA at 07:24, 18 October 2018

OCA — Thu, 18 Oct 2018 07:24:50 GMT

←Older revision		Revision as of 07:24, 18 October 2018
Line 1:		Line 1:
		+
	==Crystal structure of complex between E201A/SA-subtilisin and Tk-propeptide==		==Crystal structure of complex between E201A/SA-subtilisin and Tk-propeptide==
	<StructureSection load='3a3p' size='340' side='right' caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3a3p' size='340' side='right' caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://pdbe.org/3a3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://pdbe.org/3a3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3a3p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/3a3p_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/3a3p_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:48, 10 February 2016

OCA — Wed, 10 Feb 2016 06:48:14 GMT

←Older revision		Revision as of 06:48, 10 February 2016
Line 5:		Line 5:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://pdbe.org/3a3p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a3p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3a3p" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 12:32, 25 December 2014

OCA — Thu, 25 Dec 2014 12:32:42 GMT

←Older revision		Revision as of 12:32, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A3P FirstGlance]. <br>		<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A3P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TKSU_THEKO TKSU_THEKO]] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Thermococcus kodakaraensis atomi et al. 2004]]		[[Category: Thermococcus kodakaraensis atomi et al. 2004]]
-	[[Category: Kanaya, S.]]	+	[[Category: Kanaya, S]]
-	[[Category: Koga, Y.]]	+	[[Category: Koga, Y]]
-	[[Category: Matsumura, H.]]	+	[[Category: Matsumura, H]]
-	[[Category: Takano, K.]]	+	[[Category: Takano, K]]
-	[[Category: Tanaka, S.]]	+	[[Category: Tanaka, S]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Propeptide]]		[[Category: Propeptide]]

OCA at 05:32, 3 October 2014

OCA — Fri, 03 Oct 2014 05:32:24 GMT

←Older revision		Revision as of 05:32, 3 October 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3a3p\| PDB=3a3p \| SCENE= }}~~	+	==Crystal structure of complex between E201A/SA-subtilisin and Tk-propeptide==
-	===Crystal structure of complex between E201A/SA-subtilisin and Tk-propeptide===	+	<StructureSection load='3a3p' size='340' side='right' caption='[[3a3p]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_19766655}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3a3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A3P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2z30\|2z30]], [[3a3n\|3a3n]], [[3a3o\|3a3o]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a3p RCSB], [http://www.ebi.ac.uk/pdbsum/3a3p PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/3a3p_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tk-subtilisin requires Ca(2+) for folding. This folding is accelerated by the chaperone function of its propeptide (Tkpro). Several Tkpro and Tk-subtilisin derivatives were constructed to examine whether the interactions between the C-terminal extended region of Tkpro and Tk-subtilisin and Glu61/Asp63- and Glu201-mediated hydrogen bonds at the domain interface are important for the chaperone function of Tkpro. The Tkpro derivatives with a series of C-terminal truncations and double mutations at Glu61 and Asp63 exhibited weaker chaperone functions than Tkpro for SA-subtilisin (active-site mutant of Tk-subtilisin). Good correlation was observed between their chaperone functions and binding abilities to the folded SA-subtilisin protein. These results suggest that the C-terminal extended region, Glu61, and Asp63 of Tkpro are not critical for folding of Tk-subtilisin but accelerate it by binding to a folding intermediate of Tk-subtilisin with a native-like structure at their binding sites. In contrast, Tkpro exhibited little chaperone function for E201A/SA-subtilisin. It could bind to the folded E201A/SA-subtilisin protein with a lower association constant than that for SA-subtilisin. These results suggest a loop of Tkpro, which interacts with Glu201 of Tk-subtilisin through hydrogen bonds and is required for folding of Tk-subtilisin by binding to a folding intermediate of Tk-subtilisin with a nonnative structure. Because this loop is fairly hydrophobic and tightly packs to the surface parallel helices of the central alphabetaalpha substructure of Tk-subtilisin, binding of this loop to Glu201 may induce association of these two helices and thereby formation of the alphabetaalpha substructure. We propose that Glu201-mediated interactions are critical for initiation of Tkpro-catalyzed folding of Tk-subtilisin.

-	~~==Function==~~	+	Identification of the interactions critical for propeptide-catalyzed folding of Tk-subtilisin.,Tanaka S, Matsumura H, Koga Y, Takano K, Kanaya S J Mol Biol. 2009 Nov 27;394(2):306-19. doi: 10.1016/j.jmb.2009.09.028. Epub 2009 , Sep 18. PMID:19766655<ref>PMID:19766655</ref>
-	~~[[http~~:~~//www~~.~~uniprot~~.~~org~~/~~uniprot/TKSU_THEKO TKSU_THEKO]] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3a3p]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/"thermococcus_kodakaraensis"_atomi_et_al~~.~~_2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA].~~	+	</div>

	==See Also==		==See Also==
	*[[Subtilisin\|Subtilisin]]		*[[Subtilisin\|Subtilisin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:019766655</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Thermococcus kodakaraensis atomi et al. 2004]]		[[Category: Thermococcus kodakaraensis atomi et al. 2004]]
	[[Category: Kanaya, S.]]		[[Category: Kanaya, S.]]

OCA at 06:00, 22 January 2014

OCA — Wed, 22 Jan 2014 06:00:45 GMT

←Older revision		Revision as of 06:00, 22 January 2014
Line 1:		Line 1:
-	[[Image:3a3p.png\|left\|200px]]
-
	{{STRUCTURE_3a3p\| PDB=3a3p \| SCENE= }}		{{STRUCTURE_3a3p\| PDB=3a3p \| SCENE= }}
-
	===Crystal structure of complex between E201A/SA-subtilisin and Tk-propeptide===		===Crystal structure of complex between E201A/SA-subtilisin and Tk-propeptide===
		+	{{ABSTRACT_PUBMED_19766655}}

		+	==Function==
		+	[[http://www.uniprot.org/uniprot/TKSU_THEKO TKSU_THEKO]] Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position.

	==About this Structure==		==About this Structure==
-	[[3a3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermococcus_kodakarensis Thermococcus kodakarensis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA].	+	[[3a3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermococcus_kodakaraensis"_atomi_et_al._2004 "thermococcus kodakaraensis" atomi et al. 2004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA].

	==See Also==		==See Also==
	*[[Subtilisin\|Subtilisin]]		*[[Subtilisin\|Subtilisin]]
-	[[Category: Thermococcus ~~kodakarensis~~]]	+
		+	==Reference==
		+	<ref group="xtra">PMID:019766655</ref><references group="xtra"/><references/>
		+	[[Category: Thermococcus kodakaraensis atomi et al. 2004]]
	[[Category: Kanaya, S.]]		[[Category: Kanaya, S.]]
	[[Category: Koga, Y.]]		[[Category: Koga, Y.]]

OCA at 16:42, 20 October 2012

OCA — Sat, 20 Oct 2012 16:42:31 GMT

←Older revision		Revision as of 16:42, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3a3p]] is a 2 chain structure ~~of [[Subtilisin]]~~ with sequence from [http://en.wikipedia.org/wiki/Thermococcus_kodakarensis Thermococcus kodakarensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA].	+	[[3a3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_kodakarensis Thermococcus kodakarensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A3P OCA].

	==See Also==		==See Also==

OCA at 22:35, 26 July 2012

OCA — Thu, 26 Jul 2012 22:35:17 GMT

←Older revision		Revision as of 22:35, 26 July 2012
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	[[Image:3a3p.png\|left\|200px]]		[[Image:3a3p.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3a3p", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3a3p\| PDB=3a3p \| SCENE= }}		{{STRUCTURE_3a3p\| PDB=3a3p \| SCENE= }}

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	==See Also==		==See Also==
-	*[[Subtilisin]]	+	*[[Subtilisin\|Subtilisin]]
	[[Category: Thermococcus kodakarensis]]		[[Category: Thermococcus kodakarensis]]
	[[Category: Kanaya, S.]]		[[Category: Kanaya, S.]]
Line 23:		Line 17:
	[[Category: Takano, K.]]		[[Category: Takano, K.]]
	[[Category: Tanaka, S.]]		[[Category: Tanaka, S.]]
-	[[Category: Calcium]]
-	[[Category: Crystal structure]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Propeptide]]		[[Category: Propeptide]]