3a4g - Revision history

OCA at 14:10, 1 November 2023

OCA — Wed, 01 Nov 2023 14:10:18 GMT

←Older revision		Revision as of 14:10, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3a4g' size='340' side='right'caption='[[3a4g]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3a4g' size='340' side='right'caption='[[3a4g]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a4g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"streptomyces_autotrophicus"_takamiya_and_tubaki_1956 "streptomyces autotrophicus" takamiya and tubaki 1956~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A4G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a4g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A4G FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~, ~~<scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3a4h\|3a4h]], [[3a4z\|3a4z]], [[3a50\|3a50]], [[3a51\|3a51]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">vdh ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2074 "Streptomyces autotrophicus" Takamiya and Tubaki 1956])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4g OCA], [https://pdbe.org/3a4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a4g RCSB], [https://www.ebi.ac.uk/pdbsum/3a4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4g OCA], [https://pdbe.org/3a4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a4g RCSB], [https://www.ebi.ac.uk/pdbsum/3a4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CPVDH_PSEAH CPVDH_PSEAH]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces autotrophicus takamiya and tubaki 1956]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Arisawa, A]]	+	[[Category: Pseudonocardia autotrophica]]
-	[[Category: Cheon~~, W K~~]]	+	[[Category: Arisawa A]]
-	[[Category: Fujii, Y]]	+	[[Category: Cheon WK]]
-	[[Category: Tamura, T]]	+	[[Category: Fujii Y]]
-	[[Category: Yasutake, Y]]	+	[[Category: Tamura T]]
-	~~[[Category: Cytochrome p450]]~~	+	[[Category: Yasutake Y]]
-	~~[[Category: Hemoprotein]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Vitamin d3 hydroxylase~~]]	+

OCA at 16:16, 22 December 2021

OCA — Wed, 22 Dec 2021 16:16:36 GMT

←Older revision		Revision as of 16:16, 22 December 2021
Line 1:		Line 1:

	==Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)==		==Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)==
-	<StructureSection load='3a4g' size='340' side='right' caption='[[3a4g]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='3a4g' size='340' side='right'caption='[[3a4g]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a4g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"streptomyces_autotrophicus"_takamiya_and_tubaki_1956 "streptomyces autotrophicus" takamiya and tubaki 1956]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3A4G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a4g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_autotrophicus"_takamiya_and_tubaki_1956 "streptomyces autotrophicus" takamiya and tubaki 1956]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A4G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a4h\|3a4h]], [[3a4z\|3a4z]], [[3a50\|3a50]], [[3a51\|3a51]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3a4h\|3a4h]], [[3a4z\|3a4z]], [[3a50\|3a50]], [[3a51\|3a51]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vdh ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2074 "Streptomyces autotrophicus" Takamiya and Tubaki 1956])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vdh ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2074 "Streptomyces autotrophicus" Takamiya and Tubaki 1956])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3a4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4g OCA], [~~http~~://pdbe.org/3a4g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3a4g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3a4g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4g ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4g OCA], [https://pdbe.org/3a4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a4g RCSB], [https://www.ebi.ac.uk/pdbsum/3a4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4g ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Cytochrome P450\|Cytochrome P450]]	+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces autotrophicus takamiya and tubaki 1956]]		[[Category: Streptomyces autotrophicus takamiya and tubaki 1956]]
		+	[[Category: Large Structures]]
	[[Category: Arisawa, A]]		[[Category: Arisawa, A]]
	[[Category: Cheon, W K]]		[[Category: Cheon, W K]]

OCA at 07:51, 24 October 2018

OCA — Wed, 24 Oct 2018 07:51:54 GMT

←Older revision		Revision as of 07:51, 24 October 2018
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/3a4g_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/3a4g_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 04:34, 5 August 2016

OCA — Fri, 05 Aug 2016 04:34:47 GMT

←Older revision		Revision as of 04:34, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)==		==Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)==
	<StructureSection load='3a4g' size='340' side='right' caption='[[3a4g]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3a4g' size='340' side='right' caption='[[3a4g]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3a4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudonocardia_autotrophica Pseudonocardia autotrophica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3a4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_autotrophicus"_takamiya_and_tubaki_1956 "streptomyces autotrophicus" takamiya and tubaki 1956]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a4h\|3a4h]], [[3a4z\|3a4z]], [[3a50\|3a50]], [[3a51\|3a51]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a4h\|3a4h]], [[3a4z\|3a4z]], [[3a50\|3a50]], [[3a51\|3a51]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2074 ~~Pseudonocardia autotrophica~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2074 "Streptomyces autotrophicus" Takamiya and Tubaki 1956])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a4g RCSB], [http://www.ebi.ac.uk/pdbsum/3a4g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4g OCA], [http://pdbe.org/3a4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a4g RCSB], [http://www.ebi.ac.uk/pdbsum/3a4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4g ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a4g ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3a4g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Pseudonocardia autotrophica~~]]	+	[[Category: Streptomyces autotrophicus takamiya and tubaki 1956]]
	[[Category: Arisawa, A]]		[[Category: Arisawa, A]]
	[[Category: Cheon, W K]]		[[Category: Cheon, W K]]

OCA at 23:00, 24 December 2014

OCA — Wed, 24 Dec 2014 23:00:37 GMT

←Older revision		Revision as of 23:00, 24 December 2014
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Cytochrome P450\|Cytochrome P450]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Pseudonocardia autotrophica]]		[[Category: Pseudonocardia autotrophica]]
-	[[Category: Arisawa, A.]]	+	[[Category: Arisawa, A]]
-	[[Category: Cheon, W K.]]	+	[[Category: Cheon, W K]]
-	[[Category: Fujii, Y.]]	+	[[Category: Fujii, Y]]
-	[[Category: Tamura, T.]]	+	[[Category: Tamura, T]]
-	[[Category: Yasutake, Y.]]	+	[[Category: Yasutake, Y]]
	[[Category: Cytochrome p450]]		[[Category: Cytochrome p450]]
	[[Category: Hemoprotein]]		[[Category: Hemoprotein]]

OCA at 11:49, 29 October 2014

OCA — Wed, 29 Oct 2014 11:49:18 GMT

←Older revision		Revision as of 11:49, 29 October 2014
Line 1:		Line 1:
-	[[~~Image:~~3a4g.~~png~~\|~~left~~\|200px]]	+	==Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)==
		+	<StructureSection load='3a4g' size='340' side='right' caption='[[3a4g]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3a4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4G FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3a4h\|3a4h]], [[3a4z\|3a4z]], [[3a50\|3a50]], [[3a51\|3a51]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vdh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2074 Pseudonocardia autotrophica])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3a4g RCSB], [http://www.ebi.ac.uk/pdbsum/3a4g PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/3a4g_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vitamin D(3) hydroxylase (Vdh) isolated from actinomycete Pseudonocardia autotrophica is a cytochrome P450 (CYP) responsible for the biocatalytic conversion of vitamin D(3) (VD(3)) to 1alpha,25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)VD(3)) by P. autotrophica. Although its biological function is unclear, Vdh is capable of catalyzing the two-step hydroxylation of VD(3), i.e. the conversion of VD(3) to 25-hydroxyvitamin D(3) (25(OH)VD(3)) and then of 25(OH)VD(3) to 1alpha,25(OH)(2)VD(3), a hormonal form of VD(3). Here we describe the crystal structures of wild-type Vdh (Vdh-WT) in the substrate-free form and of the highly active quadruple mutant (Vdh-K1) generated by directed evolution in the substrate-free, VD(3)-bound, and 25(OH)VD(3)-bound forms. Vdh-WT exhibits an open conformation with the distal heme pocket exposed to the solvent both in the presence and absence of a substrate, whereas Vdh-K1 exhibits a closed conformation in both the substrate-free and substrate-bound forms. The results suggest that the conformational equilibrium was largely shifted toward the closed conformation by four amino acid substitutions scattered throughout the molecule. The substrate-bound structure of Vdh-K1 accommodates both VD(3) and 25(OH)VD(3) but in an anti-parallel orientation. The occurrence of the two secosteroid binding modes accounts for the regioselective sequential VD(3) hydroxylation activities. Moreover, these structures determined before and after directed evolution, together with biochemical and spectroscopic data, provide insights into how directed evolution has worked for significant enhancement of both the VD(3) 25-hydroxylase and 25(OH)VD(3) 1alpha-hydroxylase activities.

-	~~{{STRUCTURE_3a4g\| PDB=3a4g \| SCENE= }}~~	+	Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase.,Yasutake Y, Fujii Y, Nishioka T, Cheon WK, Arisawa A, Tamura T J Biol Chem. 2010 Oct 8;285(41):31193-201. Epub 2010 Jul 27. PMID:20667833<ref>PMID:20667833</ref>

-	~~===Structure~~ of ~~cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20667833}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3a4g]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA].~~	+	</StructureSection>
-		+
-	==~~See Also==~~	+
-	*[[Cytochrome P450\|Cytochrome P450]]	+
-		+
-	~~==Reference~~==	+
-	<~~ref group="xtra">PMID:020667833<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pseudonocardia autotrophica]]		[[Category: Pseudonocardia autotrophica]]
	[[Category: Arisawa, A.]]		[[Category: Arisawa, A.]]

OCA at 10:00, 20 October 2012

OCA — Sat, 20 Oct 2012 10:00:00 GMT

←Older revision		Revision as of 10:00, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3a4g]] is a 1 chain structure ~~of [[Cytochrome P450]]~~ with sequence from [http://en.wikipedia.org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA].	+	[[3a4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA].

	==See Also==		==See Also==

OCA: Protected "3a4g" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 00:39:33 GMT

Protected "3a4g" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:39, 26 July 2012

OCA at 00:39, 26 July 2012

OCA — Thu, 26 Jul 2012 00:39:32 GMT

←Older revision		Revision as of 00:39, 26 July 2012
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-	{{Seed}}
	[[Image:3a4g.png\|left\|200px]]		[[Image:3a4g.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3a4g", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3a4g\| PDB=3a4g \| SCENE= }}		{{STRUCTURE_3a4g\| PDB=3a4g \| SCENE= }}

	===Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)===		===Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_20667833}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 20667833 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_20667833}}		{{ABSTRACT_PUBMED_20667833}}

	==About this Structure==		==About this Structure==
-	~~3A4G~~ is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA].	+	[[3a4g]] is a 1 chain structure of [[Cytochrome P450]] with sequence from [http://en.wikipedia.org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA].
		+
		+	==See Also==
		+	*[[Cytochrome P450\|Cytochrome P450]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20667833~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020667833</ref><references group="xtra"/>
	[[Category: Pseudonocardia autotrophica]]		[[Category: Pseudonocardia autotrophica]]
	[[Category: Arisawa, A.]]		[[Category: Arisawa, A.]]
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	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Vitamin d3 hydroxylase]]		[[Category: Vitamin d3 hydroxylase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Nov 18 00:16:56 2010''

OCA at 21:05, 17 November 2010

OCA — Wed, 17 Nov 2010 21:05:42 GMT

←Older revision		Revision as of 21:05, 17 November 2010
Line 1:		Line 1:
	{{Seed}}		{{Seed}}
-	[[Image:3a4g.~~jpg~~\|left\|200px]]	+	[[Image:3a4g.png\|left\|200px]]

	<!--		<!--
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	===Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)===		===Structure of cytochrome P450 vdh from Pseudonocardia autotrophica (trigonal crystal form)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_20667833}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 20667833 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_20667833}}

	==About this Structure==		==About this Structure==
	3A4G is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA].		3A4G is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudonocardia_autotrophica Pseudonocardia autotrophica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4G OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:20667833</ref><references group="xtra"/>
	[[Category: Pseudonocardia autotrophica]]		[[Category: Pseudonocardia autotrophica]]
	[[Category: Arisawa, A.]]		[[Category: Arisawa, A.]]
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	[[Category: Vitamin d3 hydroxylase]]		[[Category: Vitamin d3 hydroxylase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 14 16~~:17:46 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Nov 18 00:16:56 2010''