3aic - Revision history

OCA at 14:00, 13 March 2024

OCA — Wed, 13 Mar 2024 14:00:41 GMT

←Older revision		Revision as of 14:00, 13 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.		[https://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Glucansucrase (GSase) from Streptococcus mutans is an essential agent in dental caries pathogenesis. Here, we report the crystal structure of S. mutans glycosyltransferase (GTF-SI), which synthesizes soluble and insoluble glucans and is a glycoside hydrolase (GH) family 70 GSase in the free enzyme form and in complex with acarbose and maltose. Resolution of the GTF-SI structure confirmed that the domain order of GTF-SI is circularly permuted as compared to that of GH family 13 alpha-amylases. As a result, domains A, B and IV of GTF-SI are each composed of two separate polypeptide chains. Structural comparison of GTF-SI and amylosucrase, which is closely related to GH family 13 amylases, indicated that the two enzymes share a similar transglycosylation mechanism via a glycosyl-enzyme intermediate in subsite -1. On the other hand, novel structural features were revealed in subsites +1 and +2 of GTF-SI. Trp517 provided the platform for glycosyl acceptor binding, while Tyr430, Asn481 and Ser589, which are conserved in family 70 enzymes but not in family 13 enzymes, comprised subsite +1. Based on the structure of GTF-SI and amino acid comparison of GTF-SI, GTF-I and GTF-S, Asp593 in GTF-SI appeared to be the most critical point for acceptor sugar orientation, influencing the transglycosylation specificity of GSases, that is, whether they produced insoluble glucan with alpha(1-3) glycosidic linkages or soluble glucan with alpha(1-6) linkages. The structural information derived from the current study should be extremely useful in the design of novel inhibitors that prevent the biofilm formation by GTF-SI.
-
-	Crystal Structure of Glucansucrase from the Dental Caries Pathogen Streptococcus mutans.,Ito K, Ito S, Shimamura T, Weyand S, Kawarasaki Y, Misaka T, Abe K, Kobayashi T, Cameron AD, Iwata S J Mol Biol. 2011 Feb 25. PMID:21354427<ref>PMID:21354427</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3aic" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 15:46, 4 October 2023

OCA — Wed, 04 Oct 2023 15:46:49 GMT

←Older revision		Revision as of 15:46, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='3aic' size='340' side='right'caption='[[3aic]], [[Resolution\|resolution]] 3.11Å' scene=''>		<StructureSection load='3aic' size='340' side='right'caption='[[3aic]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aic]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_25175 Atcc 25175~~]. The June 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aic]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. The June 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AIC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA~~-~~D-GLUCOSE'>GLC</scene>~~, ~~<scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.11Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PRD_900007:alpha-acarbose'>PRD_900007</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3aib\|3aib]], [[3aie\|3aie]]~~</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">gtfC, SMU_1005~~ (~~[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1309 ATCC 25175]~~)~~</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aic OCA], [https://pdbe.org/3aic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aic RCSB], [https://www.ebi.ac.uk/pdbsum/3aic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aic ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aic OCA], [https://pdbe.org/3aic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aic RCSB], [https://www.ebi.ac.uk/pdbsum/3aic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aic ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU]] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.	+	[https://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 25175]]
-	[[Category: Dextransucrase]]
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: ~~Ito, K~~]]	+	[[Category: Streptococcus mutans]]
-	[[Category: Ito~~, S~~]]	+	[[Category: Ito K]]
-	[[Category: ~~Iwata,~~ S]]	+	[[Category: Ito S]]
-	[[Category: ~~Shimamura, T~~]]	+	[[Category: Iwata S]]
-	[[Category: ~~Beta-alpha-barrel]]~~	+	[[Category: Shimamura T]]
-	~~[[Category: Transferase~~]]	+

OCA at 14:54, 29 December 2021

OCA — Wed, 29 Dec 2021 14:54:01 GMT

←Older revision		Revision as of 14:54, 29 December 2021
Line 1:		Line 1:
-	~~{{Large structure}}~~	+
	==Crystal Structure of Glucansucrase from Streptococcus mutans==		==Crystal Structure of Glucansucrase from Streptococcus mutans==
-	<StructureSection load='3aic' size='340' side='right' caption='[[3aic]], [[Resolution\|resolution]] 3.11Å' scene=''>	+	<StructureSection load='3aic' size='340' side='right'caption='[[3aic]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aic]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_25175 Atcc 25175]. The June 2011 RCSB PDB [~~http~~://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [~~http~~://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3AIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aic]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_25175 Atcc 25175]. The June 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AIC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~ACR~~:~~ALPHA-ACARBOSE~~'>~~ACR~~</scene>, <scene name='pdbligand=CA:~~CALCIUM+ION~~'>CA</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aib\|3aib]], [[3aie\|3aie]]</td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtfC, SMU_1005 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1309 ATCC 25175])</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3aib\|3aib]], [[3aie\|3aie]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtfC, SMU_1005 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1309 ATCC 25175])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3aic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aic OCA], [~~http~~://pdbe.org/3aic PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3aic RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3aic PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3aic ProSAT]</span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aic OCA], [https://pdbe.org/3aic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aic RCSB], [https://www.ebi.ac.uk/pdbsum/3aic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aic ProSAT]</span></td></tr>
	</table>		</table>
-	{{Large structure}}
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU]] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.	+	[[https://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU]] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 29:		Line 29:
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]
		+	[[Category: Large Structures]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Ito, K]]		[[Category: Ito, K]]

OCA at 00:23, 5 August 2016

OCA — Fri, 05 Aug 2016 00:23:47 GMT

←Older revision		Revision as of 00:23, 5 August 2016
Line 1:		Line 1:
		+	{{Large structure}}
	==Crystal Structure of Glucansucrase from Streptococcus mutans==		==Crystal Structure of Glucansucrase from Streptococcus mutans==
	<StructureSection load='3aic' size='340' side='right' caption='[[3aic]], [[Resolution\|resolution]] 3.11Å' scene=''>		<StructureSection load='3aic' size='340' side='right' caption='[[3aic]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3aic]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_mutans Streptococcus mutans~~]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AIC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3aic]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25175 Atcc 25175]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AIC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACR:ALPHA-ACARBOSE'>ACR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACR:ALPHA-ACARBOSE'>ACR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aib\|3aib]], [[3aie\|3aie]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aib\|3aib]], [[3aie\|3aie]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtfC, SMU_1005 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1309 ~~Streptococcus mutans~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtfC, SMU_1005 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1309 ATCC 25175])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aic RCSB], [http://www.ebi.ac.uk/pdbsum/3aic PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aic OCA], [http://pdbe.org/3aic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3aic RCSB], [http://www.ebi.ac.uk/pdbsum/3aic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3aic ProSAT]</span></td></tr>
	</table>		</table>
		+	{{Large structure}}
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU]] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.		[[http://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU]] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
Line 19:		Line 21:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3aic" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 25175]]
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
-	[[Category: Streptococcus mutans]]
	[[Category: Ito, K]]		[[Category: Ito, K]]
	[[Category: Ito, S]]		[[Category: Ito, S]]

OCA at 21:08, 24 December 2014

OCA — Wed, 24 Dec 2014 21:08:35 GMT

←Older revision		Revision as of 21:08, 24 December 2014
Line 1:		Line 1:
-	~~{{Large structure}}~~	+	==Crystal Structure of Glucansucrase from Streptococcus mutans==
-	~~{{STRUCTURE_3aic\| PDB~~=3aic \| ~~SCENE~~= }}	+	<StructureSection load='3aic' size='340' side='right' caption='[[3aic]], [[Resolution\|resolution]] 3.11Å' scene=''>
-	===~~Crystal Structure~~ of Glucansucrase from Streptococcus mutans===	+	== Structural highlights ==
-	~~{{ABSTRACT_PUBMED_21354427}}~~	+	<table><tr><td colspan='2'>[[3aic]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AIC FirstGlance]. <br>
-		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACR:ALPHA-ACARBOSE'>ACR</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-	==Function==	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aib\|3aib]], [[3aie\|3aie]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gtfC, SMU_1005 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1309 Streptococcus mutans])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dextransucrase Dextransucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.5 2.4.1.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aic OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aic RCSB], [http://www.ebi.ac.uk/pdbsum/3aic PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU]] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.		[[http://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU]] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glucansucrase (GSase) from Streptococcus mutans is an essential agent in dental caries pathogenesis. Here, we report the crystal structure of S. mutans glycosyltransferase (GTF-SI), which synthesizes soluble and insoluble glucans and is a glycoside hydrolase (GH) family 70 GSase in the free enzyme form and in complex with acarbose and maltose. Resolution of the GTF-SI structure confirmed that the domain order of GTF-SI is circularly permuted as compared to that of GH family 13 alpha-amylases. As a result, domains A, B and IV of GTF-SI are each composed of two separate polypeptide chains. Structural comparison of GTF-SI and amylosucrase, which is closely related to GH family 13 amylases, indicated that the two enzymes share a similar transglycosylation mechanism via a glycosyl-enzyme intermediate in subsite -1. On the other hand, novel structural features were revealed in subsites +1 and +2 of GTF-SI. Trp517 provided the platform for glycosyl acceptor binding, while Tyr430, Asn481 and Ser589, which are conserved in family 70 enzymes but not in family 13 enzymes, comprised subsite +1. Based on the structure of GTF-SI and amino acid comparison of GTF-SI, GTF-I and GTF-S, Asp593 in GTF-SI appeared to be the most critical point for acceptor sugar orientation, influencing the transglycosylation specificity of GSases, that is, whether they produced insoluble glucan with alpha(1-3) glycosidic linkages or soluble glucan with alpha(1-6) linkages. The structural information derived from the current study should be extremely useful in the design of novel inhibitors that prevent the biofilm formation by GTF-SI.

-	~~==About this~~ Structure==	+	Crystal Structure of Glucansucrase from the Dental Caries Pathogen Streptococcus mutans.,Ito K, Ito S, Shimamura T, Weyand S, Kawarasaki Y, Misaka T, Abe K, Kobayashi T, Cameron AD, Iwata S J Mol Biol. 2011 Feb 25. PMID:21354427<ref>PMID:21354427</ref>
-	~~[[3aic]] is a 8 chain structure with sequence~~ from ~~[http://en.wikipedia.org/wiki/Streptococcus_mutans~~ Streptococcus mutans]. ~~The June~~ 2011 ~~RCSB PDB [http://pdb~~.~~rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http~~:~~//dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:021354427</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]
	[[Category: RCSB PDB Molecule of the Month]]		[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Streptococcus mutans]]		[[Category: Streptococcus mutans]]
-	[[Category: Ito, K.]]	+	[[Category: Ito, K]]
-	[[Category: Ito, S.]]	+	[[Category: Ito, S]]
-	[[Category: Iwata, S.]]	+	[[Category: Iwata, S]]
-	[[Category: Shimamura, T.]]	+	[[Category: Shimamura, T]]
	[[Category: Beta-alpha-barrel]]		[[Category: Beta-alpha-barrel]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 19:26, 7 August 2013

OCA — Wed, 07 Aug 2013 19:26:18 GMT

←Older revision		Revision as of 19:26, 7 August 2013
Line 3:		Line 3:
	===Crystal Structure of Glucansucrase from Streptococcus mutans===		===Crystal Structure of Glucansucrase from Streptococcus mutans===
	{{ABSTRACT_PUBMED_21354427}}		{{ABSTRACT_PUBMED_21354427}}
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/GTFC_STRMU GTFC_STRMU]] Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and food debris.

	==About this Structure==		==About this Structure==
Line 8:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021354427</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021354427</ref><references group="xtra"/><references/>
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
	[[Category: Glucansucrase]]		[[Category: Glucansucrase]]

OCA at 08:05, 11 March 2013

OCA — Mon, 11 Mar 2013 08:05:32 GMT

←Older revision		Revision as of 08:05, 11 March 2013
Line 1:		Line 1:
-	~~[[Image:3aic.png\|left\|200px]]~~	+	{{Large structure}}
-		+
	{{STRUCTURE_3aic\| PDB=3aic \| SCENE= }}		{{STRUCTURE_3aic\| PDB=3aic \| SCENE= }}
-
	===Crystal Structure of Glucansucrase from Streptococcus mutans===		===Crystal Structure of Glucansucrase from Streptococcus mutans===
-
	{{ABSTRACT_PUBMED_21354427}}		{{ABSTRACT_PUBMED_21354427}}

OCA at 13:42, 6 February 2013

OCA — Wed, 06 Feb 2013 13:42:04 GMT

←Older revision		Revision as of 13:42, 6 February 2013
Line 1:		Line 1:
-	[[Image:3aic.~~jpg~~\|left\|200px]]	+	[[Image:3aic.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3aic", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3aic\| PDB=3aic \| SCENE= }}		{{STRUCTURE_3aic\| PDB=3aic \| SCENE= }}

	===Crystal Structure of Glucansucrase from Streptococcus mutans===		===Crystal Structure of Glucansucrase from Streptococcus mutans===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21354427}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21354427 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21354427}}		{{ABSTRACT_PUBMED_21354427}}

	==About this Structure==		==About this Structure==
-	[[3aic]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA].	+	[[3aic]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. The June 2011 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucansucrase'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2011_6 10.2210/rcsb_pdb/mom_2011_6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21354427~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021354427</ref><references group="xtra"/>
	[[Category: Dextransucrase]]		[[Category: Dextransucrase]]
		+	[[Category: Glucansucrase]]
		+	[[Category: RCSB PDB Molecule of the Month]]
	[[Category: Streptococcus mutans]]		[[Category: Streptococcus mutans]]
	[[Category: Ito, K.]]		[[Category: Ito, K.]]
Line 29:		Line 20:
	[[Category: Iwata, S.]]		[[Category: Iwata, S.]]
	[[Category: Shimamura, T.]]		[[Category: Shimamura, T.]]
		+	[[Category: Beta-alpha-barrel]]
		+	[[Category: Transferase]]

OCA at 07:08, 23 March 2011

OCA — Wed, 23 Mar 2011 07:08:08 GMT

←Older revision		Revision as of 07:08, 23 March 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3aic.jpg\|left\|200px]]

-	The ~~entry 3aic~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3aic", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3aic\| PDB=3aic \| SCENE= }}

-	~~Authors: Ito, K., Ito, S., Shimamura, T., Iwata, S.~~	+	===Crystal Structure of Glucansucrase from Streptococcus mutans===

-	~~Description~~: ~~Crystal~~ Structure ~~of Glucansucrase~~ from Streptococcus mutans	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21354427}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21354427 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21354427}}
		+
		+	==About this Structure==
		+	[[3aic]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_mutans Streptococcus mutans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIC OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21354427</ref><references group="xtra"/>
		+	[[Category: Dextransucrase]]
		+	[[Category: Streptococcus mutans]]
		+	[[Category: Ito, K.]]
		+	[[Category: Ito, S.]]
		+	[[Category: Iwata, S.]]
		+	[[Category: Shimamura, T.]]

OCA: Protected "3aic" [edit=sysop:move=sysop]

OCA — Wed, 23 Feb 2011 09:30:43 GMT

Protected "3aic" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:30, 23 February 2011