3ain - Revision history

OCA at 15:46, 4 October 2023

OCA — Wed, 04 Oct 2023 15:46:58 GMT

←Older revision		Revision as of 15:46, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='3ain' size='340' side='right'caption='[[3ain]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3ain' size='340' side='right'caption='[[3ain]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Dsm_16993 Dsm 16993~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii Sulfurisphaera tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AIN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3aik\|3aik]], [[3ail\|3ail]], [[3aim\|3aim]], [[3aio\|3aio]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST0071 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 DSM 16993])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [https://pdbe.org/3ain PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [https://www.ebi.ac.uk/pdbsum/3ain PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ain ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [https://pdbe.org/3ain PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [https://www.ebi.ac.uk/pdbsum/3ain PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ain ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q976W8_SULTO Q976W8_SULTO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Carboxylesterase]]
-	[[Category: Dsm 16993]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Angkawidjaja, C~~]]	+	[[Category: Sulfurisphaera tokodaii]]
-	[[Category: ~~Kanaya, S~~]]	+	[[Category: Angkawidjaja C]]
-	[[Category: ~~Archaea]]~~	+	[[Category: Kanaya S]]
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: R267g]]~~	+
-	~~[[Category: Thermophilic~~]]	+

OCA at 14:54, 29 December 2021

OCA — Wed, 29 Dec 2021 14:54:12 GMT

←Older revision		Revision as of 14:54, 29 December 2021
Line 1:		Line 1:

	==R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii==		==R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii==
-	<StructureSection load='3ain' size='340' side='right' caption='[[3ain]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='3ain' size='340' side='right'caption='[[3ain]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dsm_16993 Dsm 16993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3AIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Dsm_16993 Dsm 16993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AIN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aik\|3aik]], [[3ail\|3ail]], [[3aim\|3aim]], [[3aio\|3aio]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3aik\|3aik]], [[3ail\|3ail]], [[3aim\|3aim]], [[3aio\|3aio]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST0071 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 DSM 16993])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST0071 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 DSM 16993])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [~~http~~://pdbe.org/3ain PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ain PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ain ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [https://pdbe.org/3ain PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [https://www.ebi.ac.uk/pdbsum/3ain PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ain ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
	[[Category: Dsm 16993]]		[[Category: Dsm 16993]]
		+	[[Category: Large Structures]]
	[[Category: Angkawidjaja, C]]		[[Category: Angkawidjaja, C]]
	[[Category: Kanaya, S]]		[[Category: Kanaya, S]]

OCA at 23:09, 5 August 2016

OCA — Fri, 05 Aug 2016 23:09:48 GMT

←Older revision		Revision as of 23:09, 5 August 2016
Line 1:		Line 1:
		+
	==R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii==		==R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii==
	<StructureSection load='3ain' size='340' side='right' caption='[[3ain]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3ain' size='340' side='right' caption='[[3ain]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_tokodaii Sulfolobus tokodaii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AIN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_16993 Dsm 16993]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AIN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aik\|3aik]], [[3ail\|3ail]], [[3aim\|3aim]], [[3aio\|3aio]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aik\|3aik]], [[3ail\|3ail]], [[3aim\|3aim]], [[3aio\|3aio]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST0071 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 ~~Sulfolobus tokodaii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST0071 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 DSM 16993])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [http://www.ebi.ac.uk/pdbsum/3ain PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [http://pdbe.org/3ain PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [http://www.ebi.ac.uk/pdbsum/3ain PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ain ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ain" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
-	[[Category: ~~Sulfolobus tokodaii~~]]	+	[[Category: Dsm 16993]]
	[[Category: Angkawidjaja, C]]		[[Category: Angkawidjaja, C]]
	[[Category: Kanaya, S]]		[[Category: Kanaya, S]]

OCA at 23:13, 3 January 2015

OCA — Sat, 03 Jan 2015 23:13:00 GMT

←Older revision		Revision as of 23:13, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AIN FirstGlance]. <br>		<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AIN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aik\|3aik]], [[3ail\|3ail]], [[3aim\|3aim]], [[3aio\|3aio]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aik\|3aik]], [[3ail\|3ail]], [[3aim\|3aim]], [[3aio\|3aio]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST0071 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 Sulfolobus tokodaii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST0071 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 Sulfolobus tokodaii])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [http://www.ebi.ac.uk/pdbsum/3ain PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [http://www.ebi.ac.uk/pdbsum/3ain PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
	[[Category: Sulfolobus tokodaii]]		[[Category: Sulfolobus tokodaii]]
-	[[Category: Angkawidjaja, C.]]	+	[[Category: Angkawidjaja, C]]
-	[[Category: Kanaya, S.]]	+	[[Category: Kanaya, S]]
	[[Category: Archaea]]		[[Category: Archaea]]
-	[[Category: Carboxylesterase]]
	[[Category: Dimer]]		[[Category: Dimer]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: R267g]]		[[Category: R267g]]
	[[Category: Thermophilic]]		[[Category: Thermophilic]]

OCA at 06:55, 25 June 2014

OCA — Wed, 25 Jun 2014 06:55:53 GMT

←Older revision		Revision as of 06:55, 25 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3ain.~~png~~\|~~left~~\|~~200px~~]]	+	==R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii==
		+	<StructureSection load='3ain' size='340' side='right' caption='[[3ain]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ain]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AIN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3aik\|3aik]], [[3ail\|3ail]], [[3aim\|3aim]], [[3aio\|3aio]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST0071 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=111955 Sulfolobus tokodaii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxylesterase Carboxylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.1 3.1.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ain FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ain OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ain RCSB], [http://www.ebi.ac.uk/pdbsum/3ain PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The hormone-sensitive lipase (HSL) family is comprised of carboxylesterases and lipases with similarity to mammalian HSL. Thermophilic enzymes of this family have a high potential for use in biocatalysis. We prepared and crystallized a carboxylesterase of the HSL family from Sulfolobus tokodaii (Sto-Est), and determined its structures in the presence and absence of an inhibitor. Sto-Est forms a dimer in solution and the crystal structure suggests the presence of a stable biological dimer. We identified a residue close to the dimer interface, R267, which is conserved in archaeal enzymes of HSL family and is in close proximity to the same residue from the other monomer. Mutations of R267 to Glu, Gly and Lys were conducted and the resultant R267 mutants were characterized and crystallized. The structures of R267E, R267G and R267K are highly similar to that of Sto-Est with only slight differences in atomic coordinates. The dimerized states of R267E and R267G are unstable under denaturing conditions or at high temperature, as shown by a urea-induced dimer dissociation experiment and molecular dynamics simulation. R267E is the most unstable mutant protein, followed by R267G and R267K, as shown by the thermal denaturation curve and optimum temperature for activity. From the data, we discuss the importance of R267 in maintaining the dimer integrity of Sto-Est. Database The atomic coordinates and structural factors have been deposited in the Protein Data Bank with accession numbers of PDB: 3AIK for noninhibited Sto-Est, PDB: 3AIL for DEP-bound, PDB: 3AIM for R267E, PDB: 3AIN for R267G, and PDB: 3AIO for R267K Structured digital abstract * Sto-Es and Sto-Es bind by comigration in gel electrophoresis (View Interaction: 1, 2) * Sto-Es and Sto-Es bind by x-ray crystallography (View interaction).

-	~~{{STRUCTURE_3ain\| PDB=3ain \| SCENE= }}~~	+	Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii.,Angkawidjaja C, Koga Y, Takano K, Kanaya S FEBS J. 2012 Sep;279(17):3071-84. doi: 10.1111/j.1742-4658.2012.08687.x. Epub, 2012 Jul 23. PMID:22748144<ref>PMID:22748144</ref>

-	~~===R267G mutant~~ of ~~a HSL-like carboxylesterase from Sulfolobus tokodaii===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22748144}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3ain]] is a 4 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA].~~	+	</StructureSection>
	[[Category: Carboxylesterase]]		[[Category: Carboxylesterase]]
	[[Category: Sulfolobus tokodaii]]		[[Category: Sulfolobus tokodaii]]

OCA at 05:25, 5 September 2012

OCA — Wed, 05 Sep 2012 05:25:47 GMT

←Older revision		Revision as of 05:25, 5 September 2012
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-	[[Image:3ain.~~jpg~~\|left\|200px]]	+	[[Image:3ain.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ain", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ain\| PDB=3ain \| SCENE= }}		{{STRUCTURE_3ain\| PDB=3ain \| SCENE= }}

	===R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii===		===R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii===

		+	{{ABSTRACT_PUBMED_22748144}}

	==About this Structure==		==About this Structure==
Line 18:		Line 13:
	[[Category: Angkawidjaja, C.]]		[[Category: Angkawidjaja, C.]]
	[[Category: Kanaya, S.]]		[[Category: Kanaya, S.]]
		+	[[Category: Archaea]]
		+	[[Category: Carboxylesterase]]
		+	[[Category: Dimer]]
		+	[[Category: Hydrolase]]
		+	[[Category: R267g]]
		+	[[Category: Thermophilic]]

OCA: Protected "3ain" [edit=sysop:move=sysop]

OCA — Wed, 08 Jun 2011 05:17:15 GMT

Protected "3ain" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 8 June 2011

OCA at 05:17, 8 June 2011

OCA — Wed, 08 Jun 2011 05:17:09 GMT

←Older revision		Revision as of 05:17, 8 June 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3ain.jpg\|left\|200px]]

-	The ~~entry 3ain~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ain", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ain\| PDB=3ain \| SCENE= }}

-	~~Authors: Angkawidjaja, C., Kanaya, S.~~	+	===R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii===

-	Description: R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Jun 16 08~~:27:~~07 2010''~~	+	==About this Structure==
		+	[[3ain]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AIN OCA].
		+	[[Category: Carboxylesterase]]
		+	[[Category: Sulfolobus tokodaii]]
		+	[[Category: Angkawidjaja, C.]]
		+	[[Category: Kanaya, S.]]

OCA at 04:22, 16 June 2010

OCA — Wed, 16 Jun 2010 04:22:05 GMT

←Older revision		Revision as of 04:22, 16 June 2010
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ain is ON HOLD	+	The entry 3ain is ON HOLD until Paper Publication

	Authors: Angkawidjaja, C., Kanaya, S.		Authors: Angkawidjaja, C., Kanaya, S.
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	Description: R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii		Description: R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Jun ~~10 10~~:56:22 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 16 08:27:07 2010''

OCA at 06:51, 10 June 2010

OCA — Thu, 10 Jun 2010 06:51:35 GMT

←Older revision		Revision as of 06:51, 10 June 2010
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	The entry 3ain is ON HOLD		The entry 3ain is ON HOLD

-	Authors: Angkawidjaja, C, Kanaya, S	+	Authors: Angkawidjaja, C., Kanaya, S.

	Description: R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii		Description: R267G mutant of a HSL-like carboxylesterase from Sulfolobus tokodaii

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed May 26 08~~:13:47 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 10 10:56:22 2010''