3apt - Revision history

OCA at 15:51, 4 October 2023

OCA — Wed, 04 Oct 2023 15:51:40 GMT

←Older revision		Revision as of 15:51, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='3apt' size='340' side='right'caption='[[3apt]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3apt' size='340' side='right'caption='[[3apt]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3APT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3APT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~, ~~<scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3apy\|3apy]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>TTHA0327 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~300852 THET8])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[https://en.wikipedia.org/wiki/Methylenetetrahydrofolate_reductase_(NAD(P)H) Methylenetetrahydrofolate reductase (NAD(P)H)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.20 1.5.1.20] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [https://pdbe.org/3apt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [https://www.ebi.ac.uk/pdbsum/3apt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3apt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [https://pdbe.org/3apt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [https://www.ebi.ac.uk/pdbsum/3apt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3apt ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5SLG6_THET8 Q5SLG6_THET8]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3apt" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3apt" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Methylenetetrahydrofolate reductase 3D structures\|Methylenetetrahydrofolate reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thet8~~]]	+	[[Category: Thermus thermophilus HB8]]
-	[[Category: Yamada, K]]	+	[[Category: Yamada K]]
-	~~[[Category: Flavin]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 15:00, 29 December 2021

OCA — Wed, 29 Dec 2021 15:00:30 GMT

←Older revision		Revision as of 15:00, 29 December 2021
Line 1:		Line 1:

	==properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8==		==properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8==
-	<StructureSection load='3apt' size='340' side='right' caption='[[3apt]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3apt' size='340' side='right'caption='[[3apt]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3APT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3APT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3apy\|3apy]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3apy\|3apy]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0327 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0327 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Methylenetetrahydrofolate_reductase_(NAD(P)H) Methylenetetrahydrofolate reductase (NAD(P)H)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.20 1.5.1.20] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Methylenetetrahydrofolate_reductase_(NAD(P)H) Methylenetetrahydrofolate reductase (NAD(P)H)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.20 1.5.1.20] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [~~http~~://pdbe.org/3apt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3apt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3apt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [https://pdbe.org/3apt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [https://www.ebi.ac.uk/pdbsum/3apt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3apt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Thet8]]		[[Category: Thet8]]
	[[Category: Yamada, K]]		[[Category: Yamada, K]]

OCA at 17:25, 4 August 2016

OCA — Thu, 04 Aug 2016 17:25:59 GMT

←Older revision		Revision as of 17:25, 4 August 2016
Line 1:		Line 1:
		+
	==properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8==		==properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8==
	<StructureSection load='3apt' size='340' side='right' caption='[[3apt]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3apt' size='340' side='right' caption='[[3apt]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus Thermus thermophilus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3APT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3APT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3apy\|3apy]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3apy\|3apy]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0327 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~274 Thermus thermophilus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0327 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_reductase_(NAD(P)H) Methylenetetrahydrofolate reductase (NAD(P)H)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.20 1.5.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_reductase_(NAD(P)H) Methylenetetrahydrofolate reductase (NAD(P)H)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.20 1.5.1.20] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [http://www.ebi.ac.uk/pdbsum/3apt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [http://pdbe.org/3apt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [http://www.ebi.ac.uk/pdbsum/3apt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3apt ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3apt" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermus thermophilus~~]]	+	[[Category: Thet8]]
	[[Category: Yamada, K]]		[[Category: Yamada, K]]
	[[Category: Flavin]]		[[Category: Flavin]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 21:41, 3 January 2015

OCA — Sat, 03 Jan 2015 21:41:51 GMT

←Older revision		Revision as of 21:41, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3APT FirstGlance]. <br>		<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3APT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3apy\|3apy]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3apy\|3apy]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0327 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0327 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_reductase_(NAD(P)H) Methylenetetrahydrofolate reductase (NAD(P)H)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.20 1.5.1.20] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_reductase_(NAD(P)H) Methylenetetrahydrofolate reductase (NAD(P)H)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.20 1.5.1.20] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [http://www.ebi.ac.uk/pdbsum/3apt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [http://www.ebi.ac.uk/pdbsum/3apt PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	Properties and Crystal Structure of Methylenetetrahydrofolate Reductase from Thermus thermophilus HB8.,Igari S, Ohtaki A, Yamanaka Y, Sato Y, Yohda M, Odaka M, Noguchi K, Yamada K PLoS One. 2011;6(8):e23716. Epub 2011 Aug 15. PMID:21858212<ref>PMID:21858212</ref>		Properties and Crystal Structure of Methylenetetrahydrofolate Reductase from Thermus thermophilus HB8.,Igari S, Ohtaki A, Yamanaka Y, Sato Y, Yohda M, Odaka M, Noguchi K, Yamada K PLoS One. 2011;6(8):e23716. Epub 2011 Aug 15. PMID:21858212<ref>PMID:21858212</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
-	[[Category: Yamada, K.]]	+	[[Category: Yamada, K]]
	[[Category: Flavin]]		[[Category: Flavin]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 11:03, 14 May 2014

OCA — Wed, 14 May 2014 11:03:16 GMT

←Older revision		Revision as of 11:03, 14 May 2014
Line 1:		Line 1:
-	[[~~Image:~~3apt.~~jpg~~\|~~left~~\|~~200px~~]]	+	==properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8==
		+	<StructureSection load='3apt' size='340' side='right' caption='[[3apt]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3apt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3APT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3apy\|3apy]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0327 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methylenetetrahydrofolate_reductase_(NAD(P)H) Methylenetetrahydrofolate reductase (NAD(P)H)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.20 1.5.1.20] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3apt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3apt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3apt RCSB], [http://www.ebi.ac.uk/pdbsum/3apt PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Methylenetetrahydrofolate reductase (MTHFR) is one of the enzymes involved in homocysteine metabolism. Despite considerable genetic and clinical attention, the reaction mechanism and regulation of this enzyme are not fully understood because of difficult production and poor stability. While recombinant enzymes from thermophilic organisms are often stable and easy to prepare, properties of thermostable MTHFRs have not yet been reported. METHODOLOGY/PRINCIPAL FINDINGS: MTHFR from Thermus thermophilus HB8, a homologue of Escherichia coli MetF, has been expressed in E. coli and purified. The purified MTHFR was chiefly obtained as a heterodimer of apo- and holo-subunits, that is, one flavin adenine dinucleotide (FAD) prosthetic group bound per dimer. The crystal structure of the holo-subunit was quite similar to the beta(8)alpha(8) barrel of E. coli MTHFR, while that of the apo-subunit was a previously unobserved closed form. In addition, the intersubunit interface of the dimer in the crystals was different from any of the subunit interfaces of the tetramer of E. coli MTHFR. Free FAD could be incorporated into the apo-subunit of the purified Thermus enzyme after purification, forming a homodimer of holo-subunits. Comparison of the crystal structures of the heterodimer and the homodimer revealed different intersubunit interfaces, indicating a large conformational change upon FAD binding. Most of the biochemical properties of the heterodimer and the homodimer were the same, except that the homodimer showed approximately 50% activity per FAD-bound subunit in folate-dependent reactions. CONCLUSIONS/SIGNIFICANCE: The different intersubunit interfaces and rearrangement of subunits of Thermus MTHFR may be related to human enzyme properties, such as the allosteric regulation by S-adenosylmethionine and the enhanced instability of the Ala222Val mutant upon loss of FAD. Whereas E. coli MTHFR was the only structural model for human MTHFR to date, our findings suggest that Thermus MTHFR will be another useful model for this important enzyme.

-	~~<!--~~	+	Properties and Crystal Structure of Methylenetetrahydrofolate Reductase from Thermus thermophilus HB8.,Igari S, Ohtaki A, Yamanaka Y, Sato Y, Yohda M, Odaka M, Noguchi K, Yamada K PLoS One. 2011;6(8):e23716. Epub 2011 Aug 15. PMID:21858212<ref>PMID:21858212</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3apt"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3apt\| PDB=3apt \| SCENE= }}~~	+

-	~~===properties and crystal structure~~ of ~~methylenetetrahydrofolate reductase from Thermus thermophilus HB8===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21858212}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21858212 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21858212}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3apt]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021858212<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Yamada, K.]]		[[Category: Yamada, K.]]

OCA at 06:22, 14 September 2011

OCA — Wed, 14 Sep 2011 06:22:34 GMT

←Older revision		Revision as of 06:22, 14 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3apt.jpg\|left\|200px]]

-	The ~~entry 3apt~~ is ~~ON HOLD until sometime in~~ the ~~future~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3apt", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3apt\| PDB=3apt \| SCENE= }}

-	~~Authors: Yamada, K.~~	+	===properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8===

-	~~Description~~: ~~properties and crystal~~ structure ~~of methylenetetrahydrofolate reductase~~ from Thermus thermophilus ~~HB8~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21858212}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21858212 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21858212}}
		+
		+	==About this Structure==
		+	[[3apt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3APT OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021858212</ref><references group="xtra"/>
		+	[[Category: Thermus thermophilus]]
		+	[[Category: Yamada, K.]]
		+	[[Category: Flavin]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Tim barrel]]

OCA: Protected "3apt" [edit=sysop:move=sysop]

OCA — Wed, 05 Jan 2011 15:48:09 GMT

Protected "3apt" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:48, 5 January 2011

OCA at 15:48, 5 January 2011

OCA — Wed, 05 Jan 2011 15:48:08 GMT

←Older revision		Revision as of 15:48, 5 January 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3apt is ON HOLD	+	The entry 3apt is ON HOLD until sometime in the future

	Authors: Yamada, K.		Authors: Yamada, K.

	Description: properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8		Description: properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Nov 18 00:27:58 2010''

OCA at 21:16, 17 November 2010

OCA — Wed, 17 Nov 2010 21:16:43 GMT

←Older revision		Revision as of 21:16, 17 November 2010
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	The entry 3apt is ON HOLD		The entry 3apt is ON HOLD

-	Authors: ~~YAMADA~~, K	+	Authors: Yamada, K.

	Description: properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8		Description: properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed~~ Nov ~~3 09~~:58:35 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Nov 18 00:27:58 2010''

OCA: New page: '''Unreleased structure''' The entry 3apt is ON HOLD Authors: YAMADA, K Description: properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus H...

OCA — Wed, 03 Nov 2010 06:47:56 GMT

New page: '''Unreleased structure''' The entry 3apt is ON HOLD Authors: YAMADA, K Description: properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus H...

New page

'''Unreleased structure'''

The entry 3apt is ON HOLD

Authors: YAMADA, K

Description: properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 3 09:58:35 2010''